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Protein

U6 snRNA-associated Sm-like protein LSm8

Gene

LSM8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed:28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed:10523320).2 Publications

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • U6 snRNA binding Source: UniProtKB

GO - Biological processi

  • mRNA splicing, via spliceosome Source: UniProtKB
  • RNA metabolic process Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
U6 snRNA-associated Sm-like protein LSm8
Gene namesi
Name:LSM8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000128534.7
HGNCiHGNC:20471 LSM8
MIMi607288 gene
neXtProtiNX_O95777

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000128534
PharmGKBiPA165618166

Polymorphism and mutation databases

BioMutaiNAA38

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001255822 – 96U6 snRNA-associated Sm-like protein LSm8Add BLAST95

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO95777
MaxQBiO95777
PaxDbiO95777
PeptideAtlasiO95777
PRIDEiO95777
ProteomicsDBi51040
TopDownProteomicsiO95777

PTM databases

iPTMnetiO95777
PhosphoSitePlusiO95777

Expressioni

Gene expression databases

BgeeiENSG00000128534 Expressed in 221 organ(s), highest expression level in testis
CleanExiHS_LSM8
ExpressionAtlasiO95777 baseline and differential
GenevisibleiO95777 HS

Organism-specific databases

HPAiHPA020116

Interactioni

Subunit structurei

Component of the precatalytic spliceosome (spliceosome B complex) (PubMed:28781166). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex) (PubMed:10523320, PubMed:28781166, PubMed:26912367). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 (PubMed:26912367). LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome (PubMed:10523320, PubMed:26912367, PubMed:28781166).3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi119679, 70 interactors
CORUMiO95777
DIPiDIP-31195N
IntActiO95777, 54 interactors
MINTiO95777
STRINGi9606.ENSP00000249299

Structurei

3D structure databases

ProteinModelPortaliO95777
SMRiO95777
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the snRNP Sm proteins family.Curated

Phylogenomic databases

eggNOGiKOG1784 Eukaryota
ENOG41124H7 LUCA
GeneTreeiENSGT00730000111212
HOVERGENiHBG105578
InParanoidiO95777
KOiK12627
OMAiRGDNVCS
OrthoDBiEOG091G126L
PhylomeDBiO95777
TreeFamiTF314555

Family and domain databases

CDDicd01727 LSm8, 1 hit
InterProiView protein in InterPro
IPR034103 Lsm8
IPR001163 LSM_dom_euk/arc
IPR010920 LSM_dom_sf
PfamiView protein in Pfam
PF01423 LSM, 1 hit
SMARTiView protein in SMART
SM00651 Sm, 1 hit
SUPFAMiSSF50182 SSF50182, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O95777-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSALENYIN RTVAVITSDG RMIVGTLKGF DQTINLILDE SHERVFSSSQ
60 70 80 90
GVEQVVLGLY IVRGDNVAVI GEIDEETDSA LDLGNIRAEP LNSVAH
Length:96
Mass (Da):10,403
Last modified:January 23, 2007 - v3
Checksum:i15D26E6B75AA2EE1
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JIZ0C9JIZ0_HUMAN
U6 snRNA-associated Sm-like protein...
LSM8 hCG_14869
75Annotation score:
F2Z2Y6F2Z2Y6_HUMAN
U6 snRNA-associated Sm-like protein...
LSM8
67Annotation score:
C9JNV3C9JNV3_HUMAN
U6 snRNA-associated Sm-like protein...
LSM8
77Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182294 mRNA Translation: AAD56232.1
AC006389 Genomic DNA Translation: AAD15542.1
BC002742 mRNA Translation: AAH02742.1
BC022440 mRNA Translation: AAH22440.1
CCDSiCCDS5775.1
RefSeqiNP_057284.1, NM_016200.4
UniGeneiHs.655046

Genome annotation databases

EnsembliENST00000249299; ENSP00000249299; ENSG00000128534
GeneIDi51691
KEGGihsa:51691
UCSCiuc003vjg.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182294 mRNA Translation: AAD56232.1
AC006389 Genomic DNA Translation: AAD15542.1
BC002742 mRNA Translation: AAH02742.1
BC022440 mRNA Translation: AAH22440.1
CCDSiCCDS5775.1
RefSeqiNP_057284.1, NM_016200.4
UniGeneiHs.655046

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCRelectron microscopy7.0081-96[»]
5O9Zelectron microscopy4.50u1-96[»]
ProteinModelPortaliO95777
SMRiO95777
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119679, 70 interactors
CORUMiO95777
DIPiDIP-31195N
IntActiO95777, 54 interactors
MINTiO95777
STRINGi9606.ENSP00000249299

PTM databases

iPTMnetiO95777
PhosphoSitePlusiO95777

Polymorphism and mutation databases

BioMutaiNAA38

Proteomic databases

EPDiO95777
MaxQBiO95777
PaxDbiO95777
PeptideAtlasiO95777
PRIDEiO95777
ProteomicsDBi51040
TopDownProteomicsiO95777

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249299; ENSP00000249299; ENSG00000128534
GeneIDi51691
KEGGihsa:51691
UCSCiuc003vjg.4 human

Organism-specific databases

CTDi51691
EuPathDBiHostDB:ENSG00000128534.7
GeneCardsiLSM8
HGNCiHGNC:20471 LSM8
HPAiHPA020116
MIMi607288 gene
neXtProtiNX_O95777
OpenTargetsiENSG00000128534
PharmGKBiPA165618166
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1784 Eukaryota
ENOG41124H7 LUCA
GeneTreeiENSGT00730000111212
HOVERGENiHBG105578
InParanoidiO95777
KOiK12627
OMAiRGDNVCS
OrthoDBiEOG091G126L
PhylomeDBiO95777
TreeFamiTF314555

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

ChiTaRSiLSM8 human
GeneWikiiLSM8
GenomeRNAii51691
PROiPR:O95777
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000128534 Expressed in 221 organ(s), highest expression level in testis
CleanExiHS_LSM8
ExpressionAtlasiO95777 baseline and differential
GenevisibleiO95777 HS

Family and domain databases

CDDicd01727 LSm8, 1 hit
InterProiView protein in InterPro
IPR034103 Lsm8
IPR001163 LSM_dom_euk/arc
IPR010920 LSM_dom_sf
PfamiView protein in Pfam
PF01423 LSM, 1 hit
SMARTiView protein in SMART
SM00651 Sm, 1 hit
SUPFAMiSSF50182 SSF50182, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLSM8_HUMAN
AccessioniPrimary (citable) accession number: O95777
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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