UniProtKB - O95721 (SNP29_HUMAN)
Protein
Synaptosomal-associated protein 29
Gene
SNAP29
Organism
Homo sapiens (Human)
Status
Functioni
SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Plays also a role in ciliogenesis by regulating membrane fusions.3 Publications
GO - Molecular functioni
- SNAP receptor activity Source: GO_Central
- syntaxin binding Source: GO_Central
GO - Biological processi
- autophagosome maturation Source: UniProtKB
- autophagosome membrane docking Source: GO_Central
- cilium assembly Source: UniProtKB
- exocytosis Source: GO_Central
- membrane fusion Source: ProtInc
- neutrophil degranulation Source: Reactome
- protein transport Source: UniProtKB-KW
- synaptic vesicle fusion to presynaptic active zone membrane Source: GO_Central
- synaptic vesicle priming Source: GO_Central
- vesicle fusion Source: GO_Central
- vesicle targeting Source: ProtInc
Keywordsi
Biological process | Autophagy, Cilium biogenesis/degradation, Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-HSA-6798695 Neutrophil degranulation R-HSA-6811438 Intra-Golgi traffic |
Names & Taxonomyi
Protein namesi | Recommended name: Synaptosomal-associated protein 29ImportedShort name: SNAP-29Imported Alternative name(s): Soluble 29 kDa NSF attachment proteinImported Vesicle-membrane fusion protein SNAP-29 |
Gene namesi | Name:SNAP29Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000099940.11 |
HGNCi | HGNC:11133 SNAP29 |
MIMi | 604202 gene |
neXtProti | NX_O95721 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Peripheral membrane protein Curated
Plasma membrane
- cilium membrane 1 Publication; Peripheral membrane protein Curated
Other locations
- Cytoplasm 1 Publication
- autophagosome membrane 1 Publication; Peripheral membrane protein Curated
Note: Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic. Localizes to the ciliary pocket from where the cilium protrudes.2 Publications
Cytoskeleton
- centrosome Source: HPA
Cytosol
- cytosol Source: HPA
Golgi apparatus
- Golgi membrane Source: Reactome
Lysosome
- azurophil granule membrane Source: Reactome
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- ciliary pocket membrane Source: UniProtKB
- plasma membrane Source: GO_Central
Other locations
- autophagosome Source: UniProtKB
- autophagosome membrane Source: UniProtKB-SubCell
- cytoplasm Source: BHF-UCL
- presynapse Source: GOC
- SNARE complex Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, MembranePathology & Biotechi
Involvement in diseasei
Cerebral dysgenesis, neuropathy, ichthyosis, and palmoplantar keratoderma syndrome (CEDNIK)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma.
See also OMIM:609528Keywords - Diseasei
Ichthyosis, Neuropathy, Palmoplantar keratodermaOrganism-specific databases
DisGeNETi | 9342 |
MalaCardsi | SNAP29 |
MIMi | 609528 phenotype |
OpenTargetsi | ENSG00000099940 |
Orphaneti | 66631 CEDNIK syndrome |
PharmGKBi | PA35981 |
Polymorphism and mutation databases
BioMutai | SNAP29 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213601 | 1 – 258 | Synaptosomal-associated protein 29Add BLAST | 258 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 77 | PhosphoserineCombined sources | 1 | |
Modified residuei | 78 | PhosphoserineCombined sources | 1 | |
Modified residuei | 114 | PhosphoserineCombined sources | 1 | |
Modified residuei | 130 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 137 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 163 | PhosphoserineCombined sources | 1 | |
Modified residuei | 182 | PhosphoserineCombined sources | 1 | |
Modified residuei | 185 | PhosphoserineCombined sources | 1 | |
Modified residuei | 204 | PhosphoserineCombined sources | 1 | |
Modified residuei | 210 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O95721 |
jPOSTi | O95721 |
MaxQBi | O95721 |
PaxDbi | O95721 |
PeptideAtlasi | O95721 |
PRIDEi | O95721 |
ProteomicsDBi | 51014 |
PTM databases
iPTMneti | O95721 |
PhosphoSitePlusi | O95721 |
Expressioni
Tissue specificityi
Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.1 Publication
Gene expression databases
Bgeei | ENSG00000099940 Expressed in 229 organ(s), highest expression level in testis |
ExpressionAtlasi | O95721 baseline and differential |
Genevisiblei | O95721 HS |
Organism-specific databases
HPAi | CAB037092 HPA031823 HPA056492 |
Interactioni
Subunit structurei
Forms a SNARE complex, composed of VAMP8, SNAP29 and STX17, involved in fusion of autophagosome with lysosome (PubMed:23217709, PubMed:25686604). Interacts with multiple syntaxins including STX6 (By similarity). Interacts with EIPR1 (PubMed:27440922).By similarity3 Publications
Binary interactionsi
GO - Molecular functioni
- SNAP receptor activity Source: GO_Central
- syntaxin binding Source: GO_Central
Protein-protein interaction databases
BioGridi | 114748, 86 interactors |
CORUMi | O95721 |
DIPi | DIP-56475N |
IntActi | O95721, 60 interactors |
MINTi | O95721 |
STRINGi | 9606.ENSP00000215730 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4WY4 | X-ray | 1.40 | C | 39-116 | [»] | |
D | 194-258 | [»] | ||||
ProteinModelPortali | O95721 | |||||
SMRi | O95721 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 196 – 258 | t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST | 63 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 76 – 107 | Sequence analysisAdd BLAST | 32 |
Sequence similaritiesi
Belongs to the SNAP-25 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3065 Eukaryota ENOG410Y3Y0 LUCA |
GeneTreei | ENSGT00940000153618 |
HOGENOMi | HOG000046806 |
HOVERGENi | HBG057045 |
InParanoidi | O95721 |
KOi | K08509 |
OMAi | PRSYNPF |
OrthoDBi | 1358184at2759 |
PhylomeDBi | O95721 |
TreeFami | TF320226 |
Family and domain databases
InterProi | View protein in InterPro IPR000727 T_SNARE_dom |
SMARTi | View protein in SMART SM00397 t_SNARE, 2 hits |
PROSITEi | View protein in PROSITE PS50192 T_SNARE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O95721-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAYPKSYNP FDDDGEDEGA RPAPWRDARD LPDGPDAPAD RQQYLRQEVL
60 70 80 90 100
RRAEATAAST SRSLALMYES EKVGVASSEE LARQRGVLER TEKMVDKMDQ
110 120 130 140 150
DLKISQKHIN SIKSVFGGLV NYFKSKPVET PPEQNGTLTS QPNNRLKEAI
160 170 180 190 200
STSKEQEAKY QASHPNLRKL DDTDPVPRGA GSAMSTDAYP KNPHLRAYHQ
210 220 230 240 250
KIDSNLDELS MGLGRLKDIA LGMQTEIEEQ DDILDRLTTK VDKLDVNIKS
TERKVRQL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JAF7 | C9JAF7_HUMAN | Synaptosomal-associated protein 29 | SNAP29 | 160 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF115436 mRNA Translation: AAD11436.1 AF278704 Genomic DNA Translation: AAF91421.1 CR456582 mRNA Translation: CAG30468.1 BT007357 mRNA Translation: AAP36021.1 BC009715 mRNA Translation: AAH09715.1 |
CCDSi | CCDS13784.1 |
RefSeqi | NP_004773.1, NM_004782.3 |
UniGenei | Hs.108002 |
Genome annotation databases
Ensembli | ENST00000215730; ENSP00000215730; ENSG00000099940 |
GeneIDi | 9342 |
KEGGi | hsa:9342 |
UCSCi | uc011ahw.3 human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF115436 mRNA Translation: AAD11436.1 AF278704 Genomic DNA Translation: AAF91421.1 CR456582 mRNA Translation: CAG30468.1 BT007357 mRNA Translation: AAP36021.1 BC009715 mRNA Translation: AAH09715.1 |
CCDSi | CCDS13784.1 |
RefSeqi | NP_004773.1, NM_004782.3 |
UniGenei | Hs.108002 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4WY4 | X-ray | 1.40 | C | 39-116 | [»] | |
D | 194-258 | [»] | ||||
ProteinModelPortali | O95721 | |||||
SMRi | O95721 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 114748, 86 interactors |
CORUMi | O95721 |
DIPi | DIP-56475N |
IntActi | O95721, 60 interactors |
MINTi | O95721 |
STRINGi | 9606.ENSP00000215730 |
PTM databases
iPTMneti | O95721 |
PhosphoSitePlusi | O95721 |
Polymorphism and mutation databases
BioMutai | SNAP29 |
Proteomic databases
EPDi | O95721 |
jPOSTi | O95721 |
MaxQBi | O95721 |
PaxDbi | O95721 |
PeptideAtlasi | O95721 |
PRIDEi | O95721 |
ProteomicsDBi | 51014 |
Protocols and materials databases
DNASUi | 9342 |
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000215730; ENSP00000215730; ENSG00000099940 |
GeneIDi | 9342 |
KEGGi | hsa:9342 |
UCSCi | uc011ahw.3 human |
Organism-specific databases
CTDi | 9342 |
DisGeNETi | 9342 |
EuPathDBi | HostDB:ENSG00000099940.11 |
GeneCardsi | SNAP29 |
HGNCi | HGNC:11133 SNAP29 |
HPAi | CAB037092 HPA031823 HPA056492 |
MalaCardsi | SNAP29 |
MIMi | 604202 gene 609528 phenotype |
neXtProti | NX_O95721 |
OpenTargetsi | ENSG00000099940 |
Orphaneti | 66631 CEDNIK syndrome |
PharmGKBi | PA35981 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3065 Eukaryota ENOG410Y3Y0 LUCA |
GeneTreei | ENSGT00940000153618 |
HOGENOMi | HOG000046806 |
HOVERGENi | HBG057045 |
InParanoidi | O95721 |
KOi | K08509 |
OMAi | PRSYNPF |
OrthoDBi | 1358184at2759 |
PhylomeDBi | O95721 |
TreeFami | TF320226 |
Enzyme and pathway databases
Reactomei | R-HSA-6798695 Neutrophil degranulation R-HSA-6811438 Intra-Golgi traffic |
Miscellaneous databases
ChiTaRSi | SNAP29 human |
GeneWikii | SNAP29 |
GenomeRNAii | 9342 |
PROi | PR:O95721 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000099940 Expressed in 229 organ(s), highest expression level in testis |
ExpressionAtlasi | O95721 baseline and differential |
Genevisiblei | O95721 HS |
Family and domain databases
InterProi | View protein in InterPro IPR000727 T_SNARE_dom |
SMARTi | View protein in SMART SM00397 t_SNARE, 2 hits |
PROSITEi | View protein in PROSITE PS50192 T_SNARE, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | SNP29_HUMAN | |
Accessioni | O95721Primary (citable) accession number: O95721 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 13, 2019 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot