UniProtKB - O95600 (KLF8_HUMAN)
Protein
Krueppel-like factor 8
Gene
KLF8
Organism
Homo sapiens (Human)
Status
Functioni
Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G1 phase as a downstream target of focal adhesion kinase (FAK).3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 274 – 298 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 304 – 328 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 334 – 356 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O95600 |
SIGNORi | O95600 |
Names & Taxonomyi
Protein namesi | Recommended name: Krueppel-like factor 8Alternative name(s): Basic krueppel-like factor 3 Zinc finger protein 741 |
Gene namesi | Name:KLF8 Synonyms:BKLF3, ZNF741 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000102349.15 |
HGNCi | HGNC:6351, KLF8 |
MIMi | 300286, gene |
neXtProti | NX_O95600 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleoplasm Source: HPA
Other locations
- aggresome Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 67 | K → R: Abolishes sumoylation. No change in nuclear location. Increases transcriptional activity and cell cycle progression. Abolishes sumoylation; when associated with R-217. 1 Publication | 1 | |
Mutagenesisi | 217 | K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-67. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 11279 |
OpenTargetsi | ENSG00000102349 |
PharmGKBi | PA30141 |
Miscellaneous databases
Pharosi | O95600, Tbio |
Polymorphism and mutation databases
BioMutai | KLF8 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047176 | 1 – 359 | Krueppel-like factor 8Add BLAST | 359 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 67 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) |
Post-translational modificationi
Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G1 phase. Has no effect on subcellular location.1 Publication
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
jPOSTi | O95600 |
MassIVEi | O95600 |
PaxDbi | O95600 |
PeptideAtlasi | O95600 |
PRIDEi | O95600 |
ProteomicsDBi | 17622 50947 [O95600-1] |
PTM databases
iPTMneti | O95600 |
PhosphoSitePlusi | O95600 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000102349, Expressed in forebrain and 206 other tissues |
ExpressionAtlasi | O95600, baseline and differential |
Genevisiblei | O95600, HS |
Organism-specific databases
HPAi | ENSG00000102349, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with corepressor CtBP2.
Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 116435, 16 interactors |
ELMi | O95600 |
IntActi | O95600, 6 interactors |
MINTi | O95600 |
STRINGi | 9606.ENSP00000417303 |
Miscellaneous databases
RNActi | O95600, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 85 – 93 | 9aaTAD; inactive1 Publication | 9 |
Domaini
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF8, the motif is inactive.1 Publication
Sequence similaritiesi
Belongs to the Sp1 C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 274 – 298 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 304 – 328 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 334 – 356 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000161062 |
HOGENOMi | CLU_002678_33_0_1 |
InParanoidi | O95600 |
OMAi | KMTSPPF |
PhylomeDBi | O95600 |
TreeFami | TF350556 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O95600-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVDMDKLINN LEVQLNSEGG SMQVFKQVTA SVRNRDPPEI EYRSNMTSPT
60 70 80 90 100
LLDANPMENP ALFNDIKIEP PEELLASDFS LPQVEPVDLS FHKPKAPLQP
110 120 130 140 150
ASMLQAPIRP PKPQSSPQTL VVSTSTSDMS TSANIPTVLT PGSVLTSSQS
160 170 180 190 200
TGSQQILHVI HTIPSVSLPN KMGGLKTIPV VVQSLPMVYT TLPADGGPAA
210 220 230 240 250
ITVPLIGGDG KNAGSVKVDP TSMSPLEIPS DSEESTIESG SSALQSLQGL
260 270 280 290 300
QQEPAAMAQM QGEESLDLKR RRIHQCDFAG CSKVYTKSSH LKAHRRIHTG
310 320 330 340 350
EKPYKCTWDG CSWKFARSDE LTRHFRKHTG IKPFRCTDCN RSFSRSDHLS
LHRRRHDTM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ05BZ3 | Q05BZ3_HUMAN | KLF8 protein | KLF8 hCG_17979 | 228 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 167 | S → I in BAG58895 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 263 | E → G in AAC99849 (Ref. 1) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047480 | 1 – 27 | MVDMD…QVFKQ → MSLPEDGMSSGHFRSPQLVT WS in isoform 3. 1 PublicationAdd BLAST | 27 | |
Alternative sequenceiVSP_047481 | 216 – 299 | Missing in isoform 4. 1 PublicationAdd BLAST | 84 | |
Alternative sequenceiVSP_045460 | 254 – 359 | PAAMA…RHDTM → REAL in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 106 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28282 mRNA Translation: AAC99849.1 HF546207 mRNA Translation: CCO02793.1 HF546208 mRNA Translation: CCO02794.1 HF546209 mRNA Translation: CCO02795.1 AK296156 mRNA Translation: BAG58895.1 AB209004 mRNA Translation: BAD92241.1 Sequence problems. BX641066 mRNA Translation: CAE46033.1 Sequence problems. AL050309 Genomic DNA No translation available. BX322609 Genomic DNA No translation available. BC105130 mRNA Translation: AAI05131.1 BC112109 mRNA Translation: AAI12110.1 |
CCDSi | CCDS14373.1 [O95600-1] CCDS55428.1 [O95600-3] CCDS87749.1 [O95600-4] |
RefSeqi | NP_001152768.1, NM_001159296.2 [O95600-3] NP_001311028.1, NM_001324099.1 [O95600-4] NP_001311029.1, NM_001324100.1 [O95600-5] NP_001311031.1, NM_001324102.1 [O95600-1] NP_009181.2, NM_007250.5 [O95600-1] XP_005262034.1, XM_005261977.2 [O95600-1] XP_005262036.1, XM_005261979.3 XP_006724638.1, XM_006724575.2 [O95600-3] |
Genome annotation databases
Ensembli | ENST00000374928; ENSP00000364063; ENSG00000102349 [O95600-3] ENST00000468660; ENSP00000417303; ENSG00000102349 [O95600-1] ENST00000640927; ENSP00000492126; ENSG00000102349 [O95600-4] |
GeneIDi | 11279 |
KEGGi | hsa:11279 |
UCSCi | uc004dur.4, human [O95600-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28282 mRNA Translation: AAC99849.1 HF546207 mRNA Translation: CCO02793.1 HF546208 mRNA Translation: CCO02794.1 HF546209 mRNA Translation: CCO02795.1 AK296156 mRNA Translation: BAG58895.1 AB209004 mRNA Translation: BAD92241.1 Sequence problems. BX641066 mRNA Translation: CAE46033.1 Sequence problems. AL050309 Genomic DNA No translation available. BX322609 Genomic DNA No translation available. BC105130 mRNA Translation: AAI05131.1 BC112109 mRNA Translation: AAI12110.1 |
CCDSi | CCDS14373.1 [O95600-1] CCDS55428.1 [O95600-3] CCDS87749.1 [O95600-4] |
RefSeqi | NP_001152768.1, NM_001159296.2 [O95600-3] NP_001311028.1, NM_001324099.1 [O95600-4] NP_001311029.1, NM_001324100.1 [O95600-5] NP_001311031.1, NM_001324102.1 [O95600-1] NP_009181.2, NM_007250.5 [O95600-1] XP_005262034.1, XM_005261977.2 [O95600-1] XP_005262036.1, XM_005261979.3 XP_006724638.1, XM_006724575.2 [O95600-3] |
3D structure databases
SMRi | O95600 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116435, 16 interactors |
ELMi | O95600 |
IntActi | O95600, 6 interactors |
MINTi | O95600 |
STRINGi | 9606.ENSP00000417303 |
PTM databases
iPTMneti | O95600 |
PhosphoSitePlusi | O95600 |
Polymorphism and mutation databases
BioMutai | KLF8 |
Proteomic databases
jPOSTi | O95600 |
MassIVEi | O95600 |
PaxDbi | O95600 |
PeptideAtlasi | O95600 |
PRIDEi | O95600 |
ProteomicsDBi | 17622 50947 [O95600-1] |
Protocols and materials databases
Antibodypediai | 13002, 216 antibodies |
DNASUi | 11279 |
Genome annotation databases
Ensembli | ENST00000374928; ENSP00000364063; ENSG00000102349 [O95600-3] ENST00000468660; ENSP00000417303; ENSG00000102349 [O95600-1] ENST00000640927; ENSP00000492126; ENSG00000102349 [O95600-4] |
GeneIDi | 11279 |
KEGGi | hsa:11279 |
UCSCi | uc004dur.4, human [O95600-1] |
Organism-specific databases
CTDi | 11279 |
DisGeNETi | 11279 |
EuPathDBi | HostDB:ENSG00000102349.15 |
GeneCardsi | KLF8 |
HGNCi | HGNC:6351, KLF8 |
HPAi | ENSG00000102349, Low tissue specificity |
MIMi | 300286, gene |
neXtProti | NX_O95600 |
OpenTargetsi | ENSG00000102349 |
PharmGKBi | PA30141 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000161062 |
HOGENOMi | CLU_002678_33_0_1 |
InParanoidi | O95600 |
OMAi | KMTSPPF |
PhylomeDBi | O95600 |
TreeFami | TF350556 |
Enzyme and pathway databases
PathwayCommonsi | O95600 |
SIGNORi | O95600 |
Miscellaneous databases
BioGRID-ORCSi | 11279, 2 hits in 490 CRISPR screens |
ChiTaRSi | KLF8, human |
GeneWikii | KLF8 |
GenomeRNAii | 11279 |
Pharosi | O95600, Tbio |
PROi | PR:O95600 |
RNActi | O95600, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000102349, Expressed in forebrain and 206 other tissues |
ExpressionAtlasi | O95600, baseline and differential |
Genevisiblei | O95600, HS |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KLF8_HUMAN | |
Accessioni | O95600Primary (citable) accession number: O95600 Secondary accession number(s): B4DJN3 Q9UGC4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2001 |
Last sequence update: | November 2, 2001 | |
Last modified: | December 2, 2020 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM