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Protein

Krueppel-like factor 8

Gene

KLF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G1 phase as a downstream target of focal adhesion kinase (FAK).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri274 – 298C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri304 – 328C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri334 – 356C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: NTNU_SB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

SIGNORiO95600

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 8
Alternative name(s):
Basic krueppel-like factor 3
Zinc finger protein 741
Gene namesi
Name:KLF8
Synonyms:BKLF3, ZNF741
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000102349.15
HGNCiHGNC:6351 KLF8
MIMi300286 gene
neXtProtiNX_O95600

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67K → R: Abolishes sumoylation. No change in nuclear location. Increases transcriptional activity and cell cycle progression. Abolishes sumoylation; when associated with R-217. 1 Publication1
Mutagenesisi217K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-67. 1 Publication1

Organism-specific databases

DisGeNETi11279
OpenTargetsiENSG00000102349
PharmGKBiPA30141

Polymorphism and mutation databases

BioMutaiKLF8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471761 – 359Krueppel-like factor 8Add BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki67Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G1 phase. Has no effect on subcellular location.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO95600
PeptideAtlasiO95600
PRIDEiO95600
ProteomicsDBi50947

PTM databases

iPTMnetiO95600
PhosphoSitePlusiO95600

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000102349 Expressed in 189 organ(s), highest expression level in forebrain
CleanExiHS_KLF8
ExpressionAtlasiO95600 baseline and differential
GenevisibleiO95600 HS

Organism-specific databases

HPAiHPA071740

Interactioni

Subunit structurei

Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8.2 Publications

Protein-protein interaction databases

BioGridi116435, 14 interactors
ELMiO95600
IntActiO95600, 6 interactors
MINTiO95600
STRINGi9606.ENSP00000417303

Structurei

3D structure databases

ProteinModelPortaliO95600
SMRiO95600
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri274 – 298C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri304 – 328C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri334 – 356C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118998
HOVERGENiHBG003941
InParanoidiO95600
KOiK09205
OMAiPAAMTQM
OrthoDBiEOG091G1BN0
PhylomeDBiO95600
TreeFamiTF350556

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O95600-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVDMDKLINN LEVQLNSEGG SMQVFKQVTA SVRNRDPPEI EYRSNMTSPT
60 70 80 90 100
LLDANPMENP ALFNDIKIEP PEELLASDFS LPQVEPVDLS FHKPKAPLQP
110 120 130 140 150
ASMLQAPIRP PKPQSSPQTL VVSTSTSDMS TSANIPTVLT PGSVLTSSQS
160 170 180 190 200
TGSQQILHVI HTIPSVSLPN KMGGLKTIPV VVQSLPMVYT TLPADGGPAA
210 220 230 240 250
ITVPLIGGDG KNAGSVKVDP TSMSPLEIPS DSEESTIESG SSALQSLQGL
260 270 280 290 300
QQEPAAMAQM QGEESLDLKR RRIHQCDFAG CSKVYTKSSH LKAHRRIHTG
310 320 330 340 350
EKPYKCTWDG CSWKFARSDE LTRHFRKHTG IKPFRCTDCN RSFSRSDHLS

LHRRRHDTM
Length:359
Mass (Da):39,314
Last modified:November 2, 2001 - v2
Checksum:iF8FDCC1FD477C04F
GO
Isoform 2 (identifier: O95600-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-359: PAAMAQMQGE...SLHRRRHDTM → REAL

Show »
Length:257
Mass (Da):27,242
Checksum:i86CF13DBD7494270
GO
Isoform 3 (identifier: O95600-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: MVDMDKLINNLEVQLNSEGGSMQVFKQ → MSLPEDGMSSGHFRSPQLVTWS
     254-359: PAAMAQMQGE...SLHRRRHDTM → REAL

Show »
Length:252
Mass (Da):26,623
Checksum:i0B68B09E33AC87A8
GO
Isoform 4 (identifier: O95600-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     216-299: Missing.

Show »
Length:275
Mass (Da):30,050
Checksum:i7CB5F2431F0E06D0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q05BZ3Q05BZ3_HUMAN
KLF8 protein
KLF8 hCG_17979
228Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167S → I in BAG58895 (PubMed:14702039).Curated1
Sequence conflicti263E → G in AAC99849 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0474801 – 27MVDMD…QVFKQ → MSLPEDGMSSGHFRSPQLVT WS in isoform 3. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_047481216 – 299Missing in isoform 4. 1 PublicationAdd BLAST84
Alternative sequenceiVSP_045460254 – 359PAAMA…RHDTM → REAL in isoform 2 and isoform 3. 2 PublicationsAdd BLAST106

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28282 mRNA Translation: AAC99849.1
HF546207 mRNA Translation: CCO02793.1
HF546208 mRNA Translation: CCO02794.1
HF546209 mRNA Translation: CCO02795.1
AK296156 mRNA Translation: BAG58895.1
AB209004 mRNA Translation: BAD92241.1 Sequence problems.
BX641066 mRNA Translation: CAE46033.1 Sequence problems.
AL050309 Genomic DNA No translation available.
BX322609 Genomic DNA No translation available.
BC105130 mRNA Translation: AAI05131.1
BC112109 mRNA Translation: AAI12110.1
CCDSiCCDS14373.1 [O95600-1]
CCDS55428.1 [O95600-3]
CCDS87749.1 [O95600-4]
RefSeqiNP_001152768.1, NM_001159296.2 [O95600-3]
NP_001311028.1, NM_001324099.1 [O95600-4]
NP_001311029.1, NM_001324100.1 [O95600-5]
NP_001311031.1, NM_001324102.1 [O95600-1]
NP_009181.2, NM_007250.5 [O95600-1]
XP_005262034.1, XM_005261977.2 [O95600-1]
XP_005262036.1, XM_005261979.3
XP_006724638.1, XM_006724575.2 [O95600-3]
UniGeneiHs.646614

Genome annotation databases

EnsembliENST00000374928; ENSP00000364063; ENSG00000102349 [O95600-3]
ENST00000468660; ENSP00000417303; ENSG00000102349 [O95600-1]
ENST00000640927; ENSP00000492126; ENSG00000102349 [O95600-4]
GeneIDi11279
KEGGihsa:11279
UCSCiuc004dur.4 human [O95600-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28282 mRNA Translation: AAC99849.1
HF546207 mRNA Translation: CCO02793.1
HF546208 mRNA Translation: CCO02794.1
HF546209 mRNA Translation: CCO02795.1
AK296156 mRNA Translation: BAG58895.1
AB209004 mRNA Translation: BAD92241.1 Sequence problems.
BX641066 mRNA Translation: CAE46033.1 Sequence problems.
AL050309 Genomic DNA No translation available.
BX322609 Genomic DNA No translation available.
BC105130 mRNA Translation: AAI05131.1
BC112109 mRNA Translation: AAI12110.1
CCDSiCCDS14373.1 [O95600-1]
CCDS55428.1 [O95600-3]
CCDS87749.1 [O95600-4]
RefSeqiNP_001152768.1, NM_001159296.2 [O95600-3]
NP_001311028.1, NM_001324099.1 [O95600-4]
NP_001311029.1, NM_001324100.1 [O95600-5]
NP_001311031.1, NM_001324102.1 [O95600-1]
NP_009181.2, NM_007250.5 [O95600-1]
XP_005262034.1, XM_005261977.2 [O95600-1]
XP_005262036.1, XM_005261979.3
XP_006724638.1, XM_006724575.2 [O95600-3]
UniGeneiHs.646614

3D structure databases

ProteinModelPortaliO95600
SMRiO95600
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116435, 14 interactors
ELMiO95600
IntActiO95600, 6 interactors
MINTiO95600
STRINGi9606.ENSP00000417303

PTM databases

iPTMnetiO95600
PhosphoSitePlusiO95600

Polymorphism and mutation databases

BioMutaiKLF8

Proteomic databases

PaxDbiO95600
PeptideAtlasiO95600
PRIDEiO95600
ProteomicsDBi50947

Protocols and materials databases

DNASUi11279
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374928; ENSP00000364063; ENSG00000102349 [O95600-3]
ENST00000468660; ENSP00000417303; ENSG00000102349 [O95600-1]
ENST00000640927; ENSP00000492126; ENSG00000102349 [O95600-4]
GeneIDi11279
KEGGihsa:11279
UCSCiuc004dur.4 human [O95600-1]

Organism-specific databases

CTDi11279
DisGeNETi11279
EuPathDBiHostDB:ENSG00000102349.15
GeneCardsiKLF8
HGNCiHGNC:6351 KLF8
HPAiHPA071740
MIMi300286 gene
neXtProtiNX_O95600
OpenTargetsiENSG00000102349
PharmGKBiPA30141
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118998
HOVERGENiHBG003941
InParanoidiO95600
KOiK09205
OMAiPAAMTQM
OrthoDBiEOG091G1BN0
PhylomeDBiO95600
TreeFamiTF350556

Enzyme and pathway databases

SIGNORiO95600

Miscellaneous databases

ChiTaRSiKLF8 human
GeneWikiiKLF8
GenomeRNAii11279
PROiPR:O95600
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102349 Expressed in 189 organ(s), highest expression level in forebrain
CleanExiHS_KLF8
ExpressionAtlasiO95600 baseline and differential
GenevisibleiO95600 HS

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKLF8_HUMAN
AccessioniPrimary (citable) accession number: O95600
Secondary accession number(s): B4DJN3
, E7EQQ8, L0R3U8, L0R4U2, Q2M246, Q59GV5, Q5HYQ5, Q5JXP7, Q6MZJ7, Q9UGC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: November 2, 2001
Last modified: October 10, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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