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Protein

Long-chain-fatty-acid--CoA ligase 3

Gene

ACSL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).By similarity1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate-CoA ligase activity Source: UniProtKB-EC
  • long-chain fatty acid-CoA ligase activity Source: UniProtKB
  • protein domain specific binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04703-MONOMER
BRENDAi6.2.1.3 2681
ReactomeiR-HSA-75876 Synthesis of very long-chain fatty acyl-CoAs

Chemistry databases

SwissLipidsiSLP:000000200

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 3 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 3
Short name:
LACS 3
Gene namesi
Name:ACSL3
Synonyms:ACS3, FACL3, LACS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000123983.13
HGNCiHGNC:3570 ACSL3
MIMi602371 gene
neXtProtiNX_O95573

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 41Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini42 – 720CytoplasmicSequence analysisAdd BLAST679

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Pathology & Biotechi

Organism-specific databases

DisGeNETi2181
OpenTargetsiENSG00000123983
PharmGKBiPA27967

Chemistry databases

DrugBankiDB00159 Icosapent

Polymorphism and mutation databases

BioMutaiACSL3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931071 – 720Long-chain-fatty-acid--CoA ligase 3Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei683PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO95573
MaxQBiO95573
PaxDbiO95573
PeptideAtlasiO95573
PRIDEiO95573
ProteomicsDBi50946

PTM databases

iPTMnetiO95573
PhosphoSitePlusiO95573
SwissPalmiO95573

Expressioni

Gene expression databases

BgeeiENSG00000123983 Expressed in 233 organ(s), highest expression level in endothelial cell
CleanExiHS_ACSL3
ExpressionAtlasiO95573 baseline and differential
GenevisibleiO95573 HS

Organism-specific databases

HPAiHPA011315
HPA071021

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108477, 43 interactors
IntActiO95573, 42 interactors
MINTiO95573
STRINGi9606.ENSP00000350012

Structurei

3D structure databases

ProteinModelPortaliO95573
SMRiO95573
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1256 Eukaryota
COG1022 LUCA
GeneTreeiENSGT00930000150966
HOGENOMiHOG000159459
HOVERGENiHBG106947
InParanoidiO95573
KOiK01897
OMAiSVLPIWH
OrthoDBiEOG091G02F4
PhylomeDBiO95573
TreeFamiTF314012

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O95573-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNNHVSSKPS TMKLKHTINP ILLYFIHFLI SLYTILTYIP FYFFSESRQE
60 70 80 90 100
KSNRIKAKPV NSKPDSAYRS VNSLDGLASV LYPGCDTLDK VFTYAKNKFK
110 120 130 140 150
NKRLLGTREV LNEEDEVQPN GKIFKKVILG QYNWLSYEDV FVRAFNFGNG
160 170 180 190 200
LQMLGQKPKT NIAIFCETRA EWMIAAQACF MYNFQLVTLY ATLGGPAIVH
210 220 230 240 250
ALNETEVTNI ITSKELLQTK LKDIVSLVPR LRHIITVDGK PPTWSEFPKG
260 270 280 290 300
IIVHTMAAVE ALGAKASMEN QPHSKPLPSD IAVIMYTSGS TGLPKGVMIS
310 320 330 340 350
HSNIIAGITG MAERIPELGE EDVYIGYLPL AHVLELSAEL VCLSHGCRIG
360 370 380 390 400
YSSPQTLADQ SSKIKKGSKG DTSMLKPTLM AAVPEIMDRI YKNVMNKVSE
410 420 430 440 450
MSSFQRNLFI LAYNYKMEQI SKGRNTPLCD SFVFRKVRSL LGGNIRLLLC
460 470 480 490 500
GGAPLSATTQ RFMNICFCCP VGQGYGLTES AGAGTISEVW DYNTGRVGAP
510 520 530 540 550
LVCCEIKLKN WEEGGYFNTD KPHPRGEILI GGQSVTMGYY KNEAKTKADF
560 570 580 590 600
FEDENGQRWL CTGDIGEFEP DGCLKIIDRK KDLVKLQAGE YVSLGKVEAA
610 620 630 640 650
LKNLPLVDNI CAYANSYHSY VIGFVVPNQK ELTELARKKG LKGTWEELCN
660 670 680 690 700
SCEMENEVLK VLSEAAISAS LEKFEIPVKI RLSPEPWTPE TGLVTDAFKL
710 720
KRKELKTHYQ ADIERMYGRK
Length:720
Mass (Da):80,420
Last modified:October 3, 2006 - v3
Checksum:iAAC4B0B4543EC8DD
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JC11C9JC11_HUMAN
Long-chain-fatty-acid--CoA ligase 3
ACSL3
88Annotation score:
F5H062F5H062_HUMAN
Long-chain-fatty-acid--CoA ligase 3
ACSL3
95Annotation score:
F5GWH2F5GWH2_HUMAN
Long-chain-fatty-acid--CoA ligase 3
ACSL3
141Annotation score:
H7BYZ7H7BYZ7_HUMAN
Long-chain-fatty-acid--CoA ligase 3
ACSL3
18Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti246E → D in BAA37142 (PubMed:9177793).Curated1
Sequence conflicti246E → D in BAB72074 (PubMed:11707336).Curated1
Sequence conflicti246E → D in BAB72139 (PubMed:11707336).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_026716551F → S2 PublicationsCorresponds to variant dbSNP:rs1046032Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89053 mRNA Translation: BAA37142.1
AB061712 mRNA Translation: BAB72074.1
AB061436 Genomic DNA Translation: BAB72139.1
BC041692 mRNA Translation: AAH41692.1
CCDSiCCDS2455.1
RefSeqiNP_004448.2, NM_004457.3
NP_976251.1, NM_203372.1
XP_016859073.1, XM_017003584.1
UniGeneiHs.655772

Genome annotation databases

EnsembliENST00000357430; ENSP00000350012; ENSG00000123983
ENST00000392066; ENSP00000375918; ENSG00000123983
GeneIDi2181
KEGGihsa:2181
UCSCiuc002vni.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89053 mRNA Translation: BAA37142.1
AB061712 mRNA Translation: BAB72074.1
AB061436 Genomic DNA Translation: BAB72139.1
BC041692 mRNA Translation: AAH41692.1
CCDSiCCDS2455.1
RefSeqiNP_004448.2, NM_004457.3
NP_976251.1, NM_203372.1
XP_016859073.1, XM_017003584.1
UniGeneiHs.655772

3D structure databases

ProteinModelPortaliO95573
SMRiO95573
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108477, 43 interactors
IntActiO95573, 42 interactors
MINTiO95573
STRINGi9606.ENSP00000350012

Chemistry databases

DrugBankiDB00159 Icosapent
SwissLipidsiSLP:000000200

PTM databases

iPTMnetiO95573
PhosphoSitePlusiO95573
SwissPalmiO95573

Polymorphism and mutation databases

BioMutaiACSL3

Proteomic databases

EPDiO95573
MaxQBiO95573
PaxDbiO95573
PeptideAtlasiO95573
PRIDEiO95573
ProteomicsDBi50946

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357430; ENSP00000350012; ENSG00000123983
ENST00000392066; ENSP00000375918; ENSG00000123983
GeneIDi2181
KEGGihsa:2181
UCSCiuc002vni.4 human

Organism-specific databases

CTDi2181
DisGeNETi2181
EuPathDBiHostDB:ENSG00000123983.13
GeneCardsiACSL3
HGNCiHGNC:3570 ACSL3
HPAiHPA011315
HPA071021
MIMi602371 gene
neXtProtiNX_O95573
OpenTargetsiENSG00000123983
PharmGKBiPA27967
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1256 Eukaryota
COG1022 LUCA
GeneTreeiENSGT00930000150966
HOGENOMiHOG000159459
HOVERGENiHBG106947
InParanoidiO95573
KOiK01897
OMAiSVLPIWH
OrthoDBiEOG091G02F4
PhylomeDBiO95573
TreeFamiTF314012

Enzyme and pathway databases

BioCyciMetaCyc:HS04703-MONOMER
BRENDAi6.2.1.3 2681
ReactomeiR-HSA-75876 Synthesis of very long-chain fatty acyl-CoAs

Miscellaneous databases

ChiTaRSiACSL3 human
GeneWikiiACSL3
GenomeRNAii2181
PROiPR:O95573
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123983 Expressed in 233 organ(s), highest expression level in endothelial cell
CleanExiHS_ACSL3
ExpressionAtlasiO95573 baseline and differential
GenevisibleiO95573 HS

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACSL3_HUMAN
AccessioniPrimary (citable) accession number: O95573
Secondary accession number(s): Q60I92, Q8IUM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 3, 2006
Last modified: November 7, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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