CTDNEP1

Functionⁱ

Serine/threonine protein phosphatase forming with CNEP1R1 an active phosphatase complex that dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at different levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. May antagonize BMP signaling.2 Publications

Catalytic activityⁱ

[a protein]-serine/threonine phosphate + H₂O = [a protein]-serine/threonine + phosphate.

Kineticsⁱ

K_M=
18 mM for p-nitrophenylphosphate
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "A conserved phosphatase cascade that regulates nuclear membrane biogenesis."
  Kim Y., Gentry M.S., Harris T.E., Wiley S.E., Lawrence J.C. Jr., Dixon J.E.
  Proc. Natl. Acad. Sci. U.S.A. 104:6596-6601(2007) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ASP-67, SUBCELLULAR LOCATION.

pH dependenceⁱ

Optimum pH is 5.5.1 Publication

GO - Molecular functionⁱ

phosphoprotein phosphatase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein serine/threonine phosphatase activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17420445

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

gamete generation Source: Ensembl
mesoderm development Source: Ensembl
mitotic nuclear envelope disassembly Source: Reactome
nuclear envelope organization
Source: UniProtKB
Inferred from direct assayⁱ
- 17420445
positive regulation of canonical Wnt signaling pathway Source: Ensembl
positive regulation of triglyceride biosynthetic process
Source: UniProtKB
Inferred from genetic interactionⁱ
- 22134922
protein dephosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 17420445
- 22134922
protein localization to nucleus
Source: UniProtKB
Inferred from direct assayⁱ
- 22134922

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protein phosphatase

Molecular function

Hydrolase, Protein phosphatase

Enzyme and pathway databases

Reactomeⁱ	R-HSA-4419969 Depolymerisation of the Nuclear Lamina
SABIO-RKⁱ	O95476
SignaLinkⁱ	O95476
SIGNORⁱ	O95476

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: CTD nuclear envelope phosphatase 1 (EC:3.1.3.16) Alternative name(s): Serine/threonine-protein phosphatase dullard
Gene namesⁱ	Name:CTDNEP1 Synonyms:DULLARD
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 17

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000175826.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:19085 CTDNEP1
MIMⁱ	610684 gene
neXtProtⁱ	NX_O95476

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	7 – 29	HelicalSequence analysisAdd BLAST		23

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	67	D → N or E: Abolishes phosphatase activity. 1 Publication		1

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000175826
PharmGKBⁱ	PA134937999

Polymorphism and mutation databases

BioMutaⁱ	CTDNEP1

BioMutaⁱ

CTDNEP1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000297967	1 – 244	CTD nuclear envelope phosphatase 1Add BLAST		244

Proteomic databases

EPDⁱ	O95476
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O95476
PaxDbⁱ	O95476
PeptideAtlas More...PeptideAtlasⁱ	O95476
PRIDEⁱ	O95476
ProteomicsDBⁱ	50907
TopDownProteomicsⁱ	O95476

PTM databases

DEPODⁱ	O95476
iPTMnetⁱ	O95476
PhosphoSitePlusⁱ	O95476

Expressionⁱ

Tissue specificityⁱ

Muscle specific with lower expression in other metabolic tissues.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000175826 Expressed in 231 organ(s), highest expression level in muscle of leg
CleanExⁱ	HS_DULLARD
ExpressionAtlasⁱ	O95476 baseline and differential
Genevisibleⁱ	O95476 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA037439 HPA066466

HPAⁱ

HPA037439
HPA066466

Interactionⁱ

Subunit structureⁱ

Interacts with CNEP1R1; the complex dephosphorylates LPIN1 and LPIN2.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
CNEP1R1	Q8N9A8	4	EBI-5323433,EBI-5323455
M	Q9WH76	3	EBI-5323433,EBI-10823897	From Chandipura virus (strain I653514).

Protein-protein interaction databases

BioGridⁱ	116972, 53 interactors
IntActⁱ	O95476, 5 interactors
Molecular INTeraction database More...MINTⁱ	O95476
STRINGⁱ	9606.ENSP00000321732

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O95476
SMRⁱ	O95476
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	57 – 224	FCP1 homologyPROSITE-ProRule annotationAdd BLAST		168

Sequence similaritiesⁱ

Belongs to the dullard family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG1605 Eukaryota COG5190 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000151025
HOGENOMⁱ	HOG000236379
HOVERGENⁱ	HBG098153
InParanoidⁱ	O95476
KEGG Orthology (KO) More...KOⁱ	K17617
OMAⁱ	SRIWGFI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0GIO
PhylomeDBⁱ	O95476
TreeFamⁱ	TF313962

Family and domain databases

Gene3Dⁱ	3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR011948 Dullard_phosphatase IPR004274 FCP1_dom IPR036412 HAD-like_sf IPR023214 HAD_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03031 NIF, 1 hit
SMARTⁱ	View protein in SMART SM00577 CPDc, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit
TIGRFAMsⁱ	TIGR02251 HIF-SF_euk, 1 hit
PROSITEⁱ	View protein in PROSITE PS50969 FCP1, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show all Align All

O95476-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MMRTQCLLGL RTFVAFAAKL WSFFIYLLRR QIRTVIQYQT VRYDILPLSP 
        60         70         80         90        100
VSRNRLAQVK RKILVLDLDE TLIHSHHDGV LRPTVRPGTP PDFILKVVID 
       110        120        130        140        150
KHPVRFFVHK RPHVDFFLEV VSQWYELVVF TASMEIYGSA VADKLDNSRS 
       160        170        180        190        200
ILKRRYYRQH CTLELGSYIK DLSVVHSDLS SIVILDNSPG AYRSHPDNAI 
       210        220        230        240 
PIKSWFSDPS DTALLNLLPM LDALRFTADV RSVLSRNLHQ HRLW

Length:244

Mass (Da):28,377

Last modified:November 3, 2009 - v2

Checksum:ⁱ062952A90F74575A

GO

Computationally mapped potential isoform sequencesⁱ

There are 6 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

I3L1D9	I3L1D9_HUMAN	CTD nuclear envelope phosphatase 1 CTD nuclear envelope phosphatase 1	CTDNEP1	202	Annotation score:
I3L4B2	I3L4B2_HUMAN	CTD nuclear envelope phosphatase 1 CTD nuclear envelope phosphatase 1	CTDNEP1	223	Annotation score:
I3L3K5	I3L3K5_HUMAN	CTD nuclear envelope phosphatase 1 CTD nuclear envelope phosphatase 1	CTDNEP1	208	Annotation score:
I3L0X6	I3L0X6_HUMAN	CTD nuclear envelope phosphatase 1 CTD nuclear envelope phosphatase 1	CTDNEP1	125	Annotation score:
I3L2R5	I3L2R5_HUMAN	CTD nuclear envelope phosphatase 1 CTD nuclear envelope phosphatase 1	CTDNEP1	111	Annotation score:
I3L4W0	I3L4W0_HUMAN	CTD nuclear envelope phosphatase 1 CTD nuclear envelope phosphatase 1	CTDNEP1	67	Annotation score:

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_034699	12	T → A2 PublicationsCorresponds to variant dbSNP:rs3744399Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ011916 mRNA Translation: CAA09865.1 AY364239 mRNA Translation: AAQ76798.1 AC003688 Genomic DNA No translation available. AC120057 Genomic DNA No translation available. CH471108 Genomic DNA Translation: EAW90233.1 CH471108 Genomic DNA Translation: EAW90235.1 CH471108 Genomic DNA Translation: EAW90236.1 BC009295 mRNA Translation: AAH09295.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS11093.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001137247.1, NM_001143775.1 NP_056158.2, NM_015343.4
UniGeneⁱ	Hs.513913

Genome annotation databases

Ensemblⁱ	ENST00000318988; ENSP00000321732; ENSG00000175826 ENST00000573600; ENSP00000461749; ENSG00000175826 ENST00000574205; ENSP00000458758; ENSG00000175826 ENST00000574322; ENSP00000460683; ENSG00000175826
GeneIDⁱ	23399
KEGGⁱ	hsa:23399
UCSC genome browser More...UCSCⁱ	uc002gfd.3 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O95476	CTD nuclear envelope phosphatase 1		MACMU		244	UniRef100_O95476
CTD nuclear envelope phosphatase 1		CALJA		244
CTD nuclear envelope phosphatase 1		PANTR		244
CTD nuclear envelope phosphatase 1		PONAB		244
Uncharacterized protein		PROCO		244
+33

Protein	Similar proteins		Species	Score	Length	Source
O95476	CTD nuclear envelope phosphatase 1		XENLA		244	UniRef90_O95476
CTD nuclear envelope phosphatase 1		XENTR		244
CTD nuclear envelope phosphatase 1		MOUSE		244
CTD nuclear envelope phosphatase 1		RAT		244
CTD nuclear envelope phosphatase 1		BOVIN		244
+119

Protein	Similar proteins		Species	Score	Length	Source
O95476	CTD nuclear envelope phosphatase 1		BOVIN		244	UniRef50_O95476
CTD nuclear envelope phosphatase 1		XENLA		244
CTD nuclear envelope phosphatase 1		MOUSE		244
CTD nuclear envelope phosphatase 1		RAT		244
CTD nuclear envelope phosphatase 1		XENTR		244
+410

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ011916 mRNA Translation: CAA09865.1 AY364239 mRNA Translation: AAQ76798.1 AC003688 Genomic DNA No translation available. AC120057 Genomic DNA No translation available. CH471108 Genomic DNA Translation: EAW90233.1 CH471108 Genomic DNA Translation: EAW90235.1 CH471108 Genomic DNA Translation: EAW90236.1 BC009295 mRNA Translation: AAH09295.1
CCDSⁱ	CCDS11093.1
RefSeqⁱ	NP_001137247.1, NM_001143775.1 NP_056158.2, NM_015343.4
UniGeneⁱ	Hs.513913

3D structure databases

ProteinModelPortalⁱ	O95476
SMRⁱ	O95476
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	116972, 53 interactors
IntActⁱ	O95476, 5 interactors
MINTⁱ	O95476
STRINGⁱ	9606.ENSP00000321732

PTM databases

DEPODⁱ	O95476
iPTMnetⁱ	O95476
PhosphoSitePlusⁱ	O95476

Polymorphism and mutation databases

BioMutaⁱ	CTDNEP1

BioMutaⁱ

CTDNEP1

Proteomic databases

EPDⁱ	O95476
MaxQBⁱ	O95476
PaxDbⁱ	O95476
PeptideAtlasⁱ	O95476
PRIDEⁱ	O95476
ProteomicsDBⁱ	50907
TopDownProteomicsⁱ	O95476

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	23399
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000318988; ENSP00000321732; ENSG00000175826 ENST00000573600; ENSP00000461749; ENSG00000175826 ENST00000574205; ENSP00000458758; ENSG00000175826 ENST00000574322; ENSP00000460683; ENSG00000175826
GeneIDⁱ	23399
KEGGⁱ	hsa:23399
UCSCⁱ	uc002gfd.3 human

Organism-specific databases

CTDⁱ	23399
EuPathDBⁱ	HostDB:ENSG00000175826.11
GeneCardsⁱ	CTDNEP1
HGNCⁱ	HGNC:19085 CTDNEP1
HPAⁱ	HPA037439 HPA066466
MIMⁱ	610684 gene
neXtProtⁱ	NX_O95476
OpenTargetsⁱ	ENSG00000175826
PharmGKBⁱ	PA134937999
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG1605 Eukaryota COG5190 LUCA
GeneTreeⁱ	ENSGT00930000151025
HOGENOMⁱ	HOG000236379
HOVERGENⁱ	HBG098153
InParanoidⁱ	O95476
KOⁱ	K17617
OMAⁱ	SRIWGFI
OrthoDBⁱ	EOG091G0GIO
PhylomeDBⁱ	O95476
TreeFamⁱ	TF313962

Enzyme and pathway databases

Reactomeⁱ	R-HSA-4419969 Depolymerisation of the Nuclear Lamina
SABIO-RKⁱ	O95476
SignaLinkⁱ	O95476
SIGNORⁱ	O95476

Miscellaneous databases

ChiTaRSⁱ	CTDNEP1 human
GenomeRNAiⁱ	23399
Protein Ontology More...PROⁱ	PR:O95476
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000175826 Expressed in 231 organ(s), highest expression level in muscle of leg
CleanExⁱ	HS_DULLARD
ExpressionAtlasⁱ	O95476 baseline and differential
Genevisibleⁱ	O95476 HS

Family and domain databases

Gene3Dⁱ	3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR011948 Dullard_phosphatase IPR004274 FCP1_dom IPR036412 HAD-like_sf IPR023214 HAD_sf
Pfamⁱ	View protein in Pfam PF03031 NIF, 1 hit
SMARTⁱ	View protein in SMART SM00577 CPDc, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit
TIGRFAMsⁱ	TIGR02251 HIF-SF_euk, 1 hit
PROSITEⁱ	View protein in PROSITE PS50969 FCP1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CNEP1_HUMAN
Accessionⁱ	O95476Primary (citable) accession number: O95476 Secondary accession number(s): D3DTN7, Q96GQ9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
	Last sequence update:	November 3, 2009
	Last modified:	November 7, 2018
	This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM

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UniProtKB - O95476 (CNEP1_HUMAN)

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CTD nuclear envelope phosphatase 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

pH dependencei

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Endoplasmic reticulum

Endoplasmic reticulum

Nucleus

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ