UniProtKB - O95476 (CNEP1_HUMAN)
Protein
CTD nuclear envelope phosphatase 1
Gene
CTDNEP1
Organism
Homo sapiens (Human)
Status
Functioni
Serine/threonine protein phosphatase forming with CNEP1R1 an active phosphatase complex that dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at different levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. May antagonize BMP signaling.2 Publications
Catalytic activityi
Kineticsi
- KM=18 mM for p-nitrophenylphosphate1 Publication
pH dependencei
Optimum pH is 5.5.1 Publication
GO - Molecular functioni
- phosphoprotein phosphatase activity Source: GO_Central
- protein serine/threonine phosphatase activity Source: UniProtKB
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- gamete generation Source: Ensembl
- mesoderm development Source: Ensembl
- mitotic nuclear envelope disassembly Source: Reactome
- nuclear envelope organization Source: UniProtKB
- positive regulation of canonical Wnt signaling pathway Source: Ensembl
- positive regulation of triglyceride biosynthetic process Source: UniProtKB
- protein dephosphorylation Source: UniProtKB
- protein localization to nucleus Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Enzyme and pathway databases
PathwayCommonsi | O95476 |
Reactomei | R-HSA-4419969, Depolymerisation of the Nuclear Lamina |
SABIO-RKi | O95476 |
SignaLinki | O95476 |
SIGNORi | O95476 |
Names & Taxonomyi
Protein namesi | Recommended name: CTD nuclear envelope phosphatase 1 (EC:3.1.3.16)Alternative name(s): Serine/threonine-protein phosphatase dullard |
Gene namesi | Name:CTDNEP1 Synonyms:DULLARD |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19085, CTDNEP1 |
MIMi | 610684, gene |
neXtProti | NX_O95476 |
VEuPathDBi | HostDB:ENSG00000175826.11 |
Subcellular locationi
Nucleus
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Nucleus
- nuclear envelope Source: UniProtKB
- nuclear membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- lipid droplet Source: HPA
- Nem1-Spo7 phosphatase complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 7 – 29 | HelicalSequence analysisAdd BLAST | 23 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 67 | D → N or E: Abolishes phosphatase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23399 |
OpenTargetsi | ENSG00000175826 |
PharmGKBi | PA134937999 |
Miscellaneous databases
Pharosi | O95476, Tbio |
Genetic variation databases
BioMutai | CTDNEP1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000297967 | 1 – 244 | CTD nuclear envelope phosphatase 1Add BLAST | 244 |
Proteomic databases
EPDi | O95476 |
jPOSTi | O95476 |
MassIVEi | O95476 |
MaxQBi | O95476 |
PaxDbi | O95476 |
PeptideAtlasi | O95476 |
PRIDEi | O95476 |
ProteomicsDBi | 50907 |
TopDownProteomicsi | O95476 |
PTM databases
DEPODi | CTDNEP1 |
iPTMneti | O95476 |
PhosphoSitePlusi | O95476 |
Expressioni
Tissue specificityi
Muscle specific with lower expression in other metabolic tissues.1 Publication
Gene expression databases
Bgeei | ENSG00000175826, Expressed in muscle of leg and 243 other tissues |
ExpressionAtlasi | O95476, baseline and differential |
Genevisiblei | O95476, HS |
Organism-specific databases
HPAi | ENSG00000175826, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Interacts with CNEP1R1; the complex dephosphorylates LPIN1 and LPIN2.
1 PublicationBinary interactionsi
Hide detailsO95476
With | #Exp. | IntAct |
---|---|---|
CNEP1R1 [Q8N9A8] | 4 | EBI-5323433,EBI-5323455 |
UBQLN2 [Q9UHD9] | 3 | EBI-5323433,EBI-947187 |
M [Q9WH76] from Chandipura virus (strain I653514). | 3 | EBI-5323433,EBI-10823897 |
Protein-protein interaction databases
BioGRIDi | 116972, 63 interactors |
IntActi | O95476, 18 interactors |
MINTi | O95476 |
STRINGi | 9606.ENSP00000461749 |
Miscellaneous databases
RNActi | O95476, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 224 | FCP1 homologyPROSITE-ProRule annotationAdd BLAST | 168 |
Sequence similaritiesi
Belongs to the dullard family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1605, Eukaryota |
GeneTreei | ENSGT00990000203506 |
InParanoidi | O95476 |
OMAi | AIPIRSW |
OrthoDBi | 1176152at2759 |
PhylomeDBi | O95476 |
TreeFami | TF313962 |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR011948, Dullard_phosphatase IPR004274, FCP1_dom IPR036412, HAD-like_sf IPR023214, HAD_sf |
Pfami | View protein in Pfam PF03031, NIF, 1 hit |
SMARTi | View protein in SMART SM00577, CPDc, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR02251, HIF-SF_euk, 1 hit |
PROSITEi | View protein in PROSITE PS50969, FCP1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
O95476-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMRTQCLLGL RTFVAFAAKL WSFFIYLLRR QIRTVIQYQT VRYDILPLSP
60 70 80 90 100
VSRNRLAQVK RKILVLDLDE TLIHSHHDGV LRPTVRPGTP PDFILKVVID
110 120 130 140 150
KHPVRFFVHK RPHVDFFLEV VSQWYELVVF TASMEIYGSA VADKLDNSRS
160 170 180 190 200
ILKRRYYRQH CTLELGSYIK DLSVVHSDLS SIVILDNSPG AYRSHPDNAI
210 220 230 240
PIKSWFSDPS DTALLNLLPM LDALRFTADV RSVLSRNLHQ HRLW
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketI3L1D9 | I3L1D9_HUMAN | CTD nuclear envelope phosphatase 1 | CTDNEP1 | 202 | Annotation score: | ||
I3L3K5 | I3L3K5_HUMAN | CTD nuclear envelope phosphatase 1 | CTDNEP1 | 208 | Annotation score: | ||
I3L4B2 | I3L4B2_HUMAN | CTD nuclear envelope phosphatase 1 | CTDNEP1 | 223 | Annotation score: | ||
I3L0X6 | I3L0X6_HUMAN | CTD nuclear envelope phosphatase 1 | CTDNEP1 | 125 | Annotation score: | ||
I3L2R5 | I3L2R5_HUMAN | CTD nuclear envelope phosphatase 1 | CTDNEP1 | 111 | Annotation score: | ||
I3L4W0 | I3L4W0_HUMAN | CTD nuclear envelope phosphatase 1 | CTDNEP1 | 67 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_034699 | 12 | T → A2 PublicationsCorresponds to variant dbSNP:rs3744399Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ011916 mRNA Translation: CAA09865.1 AY364239 mRNA Translation: AAQ76798.1 AC003688 Genomic DNA No translation available. AC120057 Genomic DNA No translation available. CH471108 Genomic DNA Translation: EAW90233.1 CH471108 Genomic DNA Translation: EAW90235.1 CH471108 Genomic DNA Translation: EAW90236.1 BC009295 mRNA Translation: AAH09295.1 |
CCDSi | CCDS11093.1 |
RefSeqi | NP_001137247.1, NM_001143775.1 NP_056158.2, NM_015343.4 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ011916 mRNA Translation: CAA09865.1 AY364239 mRNA Translation: AAQ76798.1 AC003688 Genomic DNA No translation available. AC120057 Genomic DNA No translation available. CH471108 Genomic DNA Translation: EAW90233.1 CH471108 Genomic DNA Translation: EAW90235.1 CH471108 Genomic DNA Translation: EAW90236.1 BC009295 mRNA Translation: AAH09295.1 |
CCDSi | CCDS11093.1 |
RefSeqi | NP_001137247.1, NM_001143775.1 NP_056158.2, NM_015343.4 |
3D structure databases
SMRi | O95476 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116972, 63 interactors |
IntActi | O95476, 18 interactors |
MINTi | O95476 |
STRINGi | 9606.ENSP00000461749 |
PTM databases
DEPODi | CTDNEP1 |
iPTMneti | O95476 |
PhosphoSitePlusi | O95476 |
Genetic variation databases
BioMutai | CTDNEP1 |
Proteomic databases
EPDi | O95476 |
jPOSTi | O95476 |
MassIVEi | O95476 |
MaxQBi | O95476 |
PaxDbi | O95476 |
PeptideAtlasi | O95476 |
PRIDEi | O95476 |
ProteomicsDBi | 50907 |
TopDownProteomicsi | O95476 |
Protocols and materials databases
Antibodypediai | 23976, 141 antibodies |
DNASUi | 23399 |
Genome annotation databases
Organism-specific databases
CTDi | 23399 |
DisGeNETi | 23399 |
GeneCardsi | CTDNEP1 |
HGNCi | HGNC:19085, CTDNEP1 |
HPAi | ENSG00000175826, Tissue enhanced (skeletal) |
MIMi | 610684, gene |
neXtProti | NX_O95476 |
OpenTargetsi | ENSG00000175826 |
PharmGKBi | PA134937999 |
VEuPathDBi | HostDB:ENSG00000175826.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1605, Eukaryota |
GeneTreei | ENSGT00990000203506 |
InParanoidi | O95476 |
OMAi | AIPIRSW |
OrthoDBi | 1176152at2759 |
PhylomeDBi | O95476 |
TreeFami | TF313962 |
Enzyme and pathway databases
PathwayCommonsi | O95476 |
Reactomei | R-HSA-4419969, Depolymerisation of the Nuclear Lamina |
SABIO-RKi | O95476 |
SignaLinki | O95476 |
SIGNORi | O95476 |
Miscellaneous databases
BioGRID-ORCSi | 23399, 236 hits in 879 CRISPR screens |
ChiTaRSi | CTDNEP1, human |
GenomeRNAii | 23399 |
Pharosi | O95476, Tbio |
PROi | PR:O95476 |
RNActi | O95476, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000175826, Expressed in muscle of leg and 243 other tissues |
ExpressionAtlasi | O95476, baseline and differential |
Genevisiblei | O95476, HS |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR011948, Dullard_phosphatase IPR004274, FCP1_dom IPR036412, HAD-like_sf IPR023214, HAD_sf |
Pfami | View protein in Pfam PF03031, NIF, 1 hit |
SMARTi | View protein in SMART SM00577, CPDc, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR02251, HIF-SF_euk, 1 hit |
PROSITEi | View protein in PROSITE PS50969, FCP1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CNEP1_HUMAN | |
Accessioni | O95476Primary (citable) accession number: O95476 Secondary accession number(s): D3DTN7, Q96GQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 21, 2007 |
Last sequence update: | November 3, 2009 | |
Last modified: | February 10, 2021 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families