UniProtKB - O95433 (AHSA1_HUMAN)
Activator of 90 kDa heat shock protein ATPase homolog 1
AHSA1
Functioni
Acts as a co-chaperone of HSP90AA1 (PubMed:29127155).
Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed:29127155).
Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360).
Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155).
2 PublicationsGO - Molecular functioni
- ATPase activator activity Source: UniProtKB
- cadherin binding Source: BHF-UCL
- chaperone binding Source: UniProtKB
- Hsp90 protein binding Source: UniProtKB
GO - Biological processi
- positive regulation of ATPase activity Source: UniProtKB
- protein folding Source: GO_Central
Keywordsi
Molecular function | Chaperone |
Biological process | Host-virus interaction, Stress response |
Enzyme and pathway databases
PathwayCommonsi | O95433 |
SignaLinki | O95433 |
SIGNORi | O95433 |
Protein family/group databases
TCDBi | 8.A.163.1.1, the hsp90/cdc37 (hsp90/cdc37) family |
Names & Taxonomyi
Protein namesi | Recommended name: Activator of 90 kDa heat shock protein ATPase homolog 1Short name: AHA1 Alternative name(s): p38 |
Gene namesi | Name:AHSA1 Synonyms:C14orf3 ORF Names:HSPC322 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1189, AHSA1 |
MIMi | 608466, gene |
neXtProti | NX_O95433 |
VEuPathDBi | HostDB:ENSG00000100591 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Note: May transiently interact with the endoplasmic reticulum.
Cytosol
- cytosol Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulumPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 223 | Y → E: Phosphomimetic mutant. Increases the binding to HSP90AA1 resulting in TSC1 dissociation from HSP90AA1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10598 |
OpenTargetsi | ENSG00000100591 |
PharmGKBi | PA25515 |
Miscellaneous databases
Pharosi | O95433, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3309113 |
Genetic variation databases
BioMutai | AHSA1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215820 | 1 – 338 | Activator of 90 kDa heat shock protein ATPase homolog 1Add BLAST | 338 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 182 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources | ||
Modified residuei | 193 | PhosphoserineCombined sources | 1 | |
Cross-linki | 203 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 212 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 223 | Phosphotyrosine; by ABL1 Publication | 1 | |
Modified residuei | 258 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O95433 |
jPOSTi | O95433 |
MassIVEi | O95433 |
MaxQBi | O95433 |
PaxDbi | O95433 |
PeptideAtlasi | O95433 |
PRIDEi | O95433 |
ProteomicsDBi | 50878 [O95433-1] 5208 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00030706 |
PTM databases
GlyGeni | O95433, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O95433 |
MetOSitei | O95433 |
PhosphoSitePlusi | O95433 |
SwissPalmi | O95433 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000100591, Expressed in right testis and 245 other tissues |
ExpressionAtlasi | O95433, baseline and differential |
Genevisiblei | O95433, HS |
Organism-specific databases
HPAi | ENSG00000100591, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with HSPCA/HSP90 (PubMed:12504007, PubMed:12604615).
Interacts (phosphorylated on Tyr-223) with HSP90AA1; the interaction activates HSP90AA1 ATPase activity (PubMed:27353360, PubMed:29127155).
Interacts with HSP90AB1 (By similarity).
Interacts with GCH1 (PubMed:16696853).
Interacts with SRPK1 (PubMed:19240134).
Interacts with FLCN (PubMed:27353360).
By similarity6 Publications(Microbial infection) Interacts with vesicular stomatitis virus glycoprotein (VSV G) (via cytoplasmic tail).
1 PublicationBinary interactionsi
O95433
With | #Exp. | IntAct |
---|---|---|
GCH1 [P30793] | 3 | EBI-448610,EBI-958183 |
HSP90AA1 [P07900] | 4 | EBI-448610,EBI-296047 |
HSP90AB1 [P08238] | 4 | EBI-448610,EBI-352572 |
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- chaperone binding Source: UniProtKB
- Hsp90 protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115846, 208 interactors |
IntActi | O95433, 42 interactors |
MINTi | O95433 |
STRINGi | 9606.ENSP00000216479 |
Miscellaneous databases
RNActi | O95433, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | O95433 |
SMRi | O95433 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O95433 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2936, Eukaryota |
GeneTreei | ENSGT00940000155144 |
HOGENOMi | CLU_049046_0_0_1 |
InParanoidi | O95433 |
OMAi | GGETEVC |
OrthoDBi | 1548191at2759 |
PhylomeDBi | O95433 |
TreeFami | TF313680 |
Family and domain databases
Gene3Di | 3.15.10.20, 1 hit 3.30.530.20, 1 hit |
InterProi | View protein in InterPro IPR013538, Activator_of_Hsp90_ATPase IPR036338, Aha1 IPR039981, AHSA-like IPR015310, AHSA1_N IPR023393, START-like_dom_sf |
PANTHERi | PTHR13009, PTHR13009, 1 hit |
Pfami | View protein in Pfam PF09229, Aha1_N, 1 hit PF08327, AHSA1, 1 hit |
SMARTi | View protein in SMART SM01000, Aha1_N, 1 hit |
SUPFAMi | SSF103111, SSF103111, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKWGEGDPR WIVEERADAT NVNNWHWTER DASNWSTDKL KTLFLAVQVQ
60 70 80 90 100
NEEGKCEVTE VSKLDGEASI NNRKGKLIFF YEWSVKLNWT GTSKSGVQYK
110 120 130 140 150
GHVEIPNLSD ENSVDEVEIS VSLAKDEPDT NLVALMKEEG VKLLREAMGI
160 170 180 190 200
YISTLKTEFT QGMILPTMNG ESVDPVGQPA LKTEERKAKP APSKTQARPV
210 220 230 240 250
GVKIPTCKIT LKETFLTSPE ELYRVFTTQE LVQAFTHAPA TLEADRGGKF
260 270 280 290 300
HMVDGNVSGE FTDLVPEKHI VMKWRFKSWP EGHFATITLT FIDKNGETEL
310 320 330
CMEGRGIPAP EEERTRQGWQ RYYFEGIKQT FGYGARLF
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V438 | G3V438_HUMAN | Activator of 90 kDa heat shock prot... | AHSA1 | 203 | Annotation score: | ||
H0YJG7 | H0YJG7_HUMAN | Activator of 90 kDa heat shock prot... | AHSA1 | 216 | Annotation score: | ||
H0YJU2 | H0YJU2_HUMAN | Activator of 90 kDa heat shock prot... | AHSA1 | 133 | Annotation score: | ||
G3V3W9 | G3V3W9_HUMAN | Activator of 90 kDa heat shock prot... | AHSA1 | 100 | Annotation score: | ||
H0YJ63 | H0YJ63_HUMAN | Activator of 90 kDa heat shock prot... | AHSA1 | 90 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 – 68 | EA → CL in AAF29000 (PubMed:11042152).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055797 | 283 – 338 | HFATI…GARLF → SHCHSG in isoform 2. 1 PublicationAdd BLAST | 56 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ243310 mRNA Translation: CAB45684.1 AF164791 mRNA Translation: AAF80755.1 AK300766 mRNA Translation: BAG62431.1 AK313824 mRNA Translation: BAG36559.1 AF111168 Genomic DNA Translation: AAD09623.1 CH471061 Genomic DNA Translation: EAW81291.1 BC000321 mRNA Translation: AAH00321.1 BC007398 mRNA Translation: AAH07398.2 AF161440 mRNA Translation: AAF29000.1 |
CCDSi | CCDS9863.1 [O95433-1] |
PIRi | JC7769 |
RefSeqi | NP_001308370.1, NM_001321441.1 NP_036243.1, NM_012111.2 [O95433-1] |
Genome annotation databases
Ensembli | ENST00000216479; ENSP00000216479; ENSG00000100591 ENST00000535854; ENSP00000440108; ENSG00000100591 [O95433-2] |
GeneIDi | 10598 |
KEGGi | hsa:10598 |
MANE-Selecti | ENST00000216479.8; ENSP00000216479.3; NM_012111.3; NP_036243.1 |
UCSCi | uc001xtw.4, human [O95433-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ243310 mRNA Translation: CAB45684.1 AF164791 mRNA Translation: AAF80755.1 AK300766 mRNA Translation: BAG62431.1 AK313824 mRNA Translation: BAG36559.1 AF111168 Genomic DNA Translation: AAD09623.1 CH471061 Genomic DNA Translation: EAW81291.1 BC000321 mRNA Translation: AAH00321.1 BC007398 mRNA Translation: AAH07398.2 AF161440 mRNA Translation: AAF29000.1 |
CCDSi | CCDS9863.1 [O95433-1] |
PIRi | JC7769 |
RefSeqi | NP_001308370.1, NM_001321441.1 NP_036243.1, NM_012111.2 [O95433-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X53 | NMR | - | A | 204-335 | [»] | |
7DMD | NMR | - | A | 28-162 | [»] | |
7DME | NMR | - | A | 28-335 | [»] | |
BMRBi | O95433 | |||||
SMRi | O95433 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115846, 208 interactors |
IntActi | O95433, 42 interactors |
MINTi | O95433 |
STRINGi | 9606.ENSP00000216479 |
Chemistry databases
ChEMBLi | CHEMBL3309113 |
Protein family/group databases
TCDBi | 8.A.163.1.1, the hsp90/cdc37 (hsp90/cdc37) family |
PTM databases
GlyGeni | O95433, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O95433 |
MetOSitei | O95433 |
PhosphoSitePlusi | O95433 |
SwissPalmi | O95433 |
Genetic variation databases
BioMutai | AHSA1 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00030706 |
Proteomic databases
EPDi | O95433 |
jPOSTi | O95433 |
MassIVEi | O95433 |
MaxQBi | O95433 |
PaxDbi | O95433 |
PeptideAtlasi | O95433 |
PRIDEi | O95433 |
ProteomicsDBi | 50878 [O95433-1] 5208 |
Protocols and materials databases
Antibodypediai | 54, 878 antibodies from 35 providers |
DNASUi | 10598 |
Genome annotation databases
Ensembli | ENST00000216479; ENSP00000216479; ENSG00000100591 ENST00000535854; ENSP00000440108; ENSG00000100591 [O95433-2] |
GeneIDi | 10598 |
KEGGi | hsa:10598 |
MANE-Selecti | ENST00000216479.8; ENSP00000216479.3; NM_012111.3; NP_036243.1 |
UCSCi | uc001xtw.4, human [O95433-1] |
Organism-specific databases
CTDi | 10598 |
DisGeNETi | 10598 |
GeneCardsi | AHSA1 |
HGNCi | HGNC:1189, AHSA1 |
HPAi | ENSG00000100591, Low tissue specificity |
MIMi | 608466, gene |
neXtProti | NX_O95433 |
OpenTargetsi | ENSG00000100591 |
PharmGKBi | PA25515 |
VEuPathDBi | HostDB:ENSG00000100591 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2936, Eukaryota |
GeneTreei | ENSGT00940000155144 |
HOGENOMi | CLU_049046_0_0_1 |
InParanoidi | O95433 |
OMAi | GGETEVC |
OrthoDBi | 1548191at2759 |
PhylomeDBi | O95433 |
TreeFami | TF313680 |
Enzyme and pathway databases
PathwayCommonsi | O95433 |
SignaLinki | O95433 |
SIGNORi | O95433 |
Miscellaneous databases
BioGRID-ORCSi | 10598, 23 hits in 1049 CRISPR screens |
ChiTaRSi | AHSA1, human |
EvolutionaryTracei | O95433 |
GeneWikii | AHSA1 |
GenomeRNAii | 10598 |
Pharosi | O95433, Tbio |
PROi | PR:O95433 |
RNActi | O95433, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100591, Expressed in right testis and 245 other tissues |
ExpressionAtlasi | O95433, baseline and differential |
Genevisiblei | O95433, HS |
Family and domain databases
Gene3Di | 3.15.10.20, 1 hit 3.30.530.20, 1 hit |
InterProi | View protein in InterPro IPR013538, Activator_of_Hsp90_ATPase IPR036338, Aha1 IPR039981, AHSA-like IPR015310, AHSA1_N IPR023393, START-like_dom_sf |
PANTHERi | PTHR13009, PTHR13009, 1 hit |
Pfami | View protein in Pfam PF09229, Aha1_N, 1 hit PF08327, AHSA1, 1 hit |
SMARTi | View protein in SMART SM01000, Aha1_N, 1 hit |
SUPFAMi | SSF103111, SSF103111, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AHSA1_HUMAN | |
Accessioni | O95433Primary (citable) accession number: O95433 Secondary accession number(s): B2R9L2 Q9P060 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 23, 2022 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families