UniProtKB - O95405 (ZFYV9_HUMAN)
Zinc finger FYVE domain-containing protein 9
ZFYVE9
Functioni
Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 705 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 708 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 721 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 724 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 729 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 732 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 750 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 753 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 699 – 758 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- 1-phosphatidylinositol binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- protein domain specific binding Source: CAFA
GO - Biological processi
- endocytosis Source: UniProtKB
- endosomal transport Source: GO_Central
- transforming growth factor beta receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Receptor |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O95405 |
Reactomei | R-HSA-2173788, Downregulation of TGF-beta receptor signaling [O95405-1] R-HSA-2173789, TGF-beta receptor signaling activates SMADs [O95405-1] R-HSA-3304356, SMAD2/3 Phosphorylation Motif Mutants in Cancer [O95405-1] R-HSA-3656532, TGFBR1 KD Mutants in Cancer [O95405-1] |
SignaLinki | O95405 |
SIGNORi | O95405 |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger FYVE domain-containing protein 9Alternative name(s): Mothers against decapentaplegic homolog-interacting protein Short name: Madh-interacting protein Novel serine protease Short name: NSP Receptor activation anchor Short name: hSARA Smad anchor for receptor activation |
Gene namesi | Name:ZFYVE9 Synonyms:MADHIP, SARA, SMADIP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6775, ZFYVE9 |
MIMi | 603755, gene |
neXtProti | NX_O95405 |
VEuPathDBi | HostDB:ENSG00000157077 |
Subcellular locationi
Cytoplasm and Cytosol
Endosome
Cytosol
- cytosol Source: HPA
Endosome
- early endosome Source: UniProtKB
- early endosome membrane Source: UniProtKB
Other locations
- intracellular membrane-bounded organelle Source: HPA
- protein-containing complex Source: CAFA
Keywords - Cellular componenti
Cytoplasm, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 782 | Y → A: Diminishes complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 782 | Y → E: Diminishes complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 783 | C → A: Diminishes complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 783 | C → E: Diminishes complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 788 | P → A: Diminishes complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 788 | P → E: Diminishes complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 790 | Q → A: No effect on complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 793 | Q → A: No effect on complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 805 | V → A: Diminishes complex formation with SMAD2. 1 Publication | 1 | |
Mutagenesisi | 805 | V → E: Diminishes complex formation with SMAD2. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9372 |
OpenTargetsi | ENSG00000157077 |
PharmGKBi | PA30532 |
Miscellaneous databases
Pharosi | O95405, Tbio |
Genetic variation databases
BioMutai | ZFYVE9 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098715 | 1 – 1425 | Zinc finger FYVE domain-containing protein 9Add BLAST | 1425 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 306 | PhosphoserineCombined sources | 1 | |
Modified residuei | 668 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O95405 |
jPOSTi | O95405 |
MassIVEi | O95405 |
MaxQBi | O95405 |
PaxDbi | O95405 |
PeptideAtlasi | O95405 |
PRIDEi | O95405 |
ProteomicsDBi | 50856 [O95405-1] 50857 [O95405-2] 50858 [O95405-3] |
PTM databases
GlyGeni | O95405, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O95405 |
PhosphoSitePlusi | O95405 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000157077, Expressed in frontal cortex and 205 other tissues |
Genevisiblei | O95405, HS |
Organism-specific databases
HPAi | ENSG00000157077, Low tissue specificity |
Interactioni
Subunit structurei
Interacts (via the SBD region) with SMAD2; the interaction recruits SMAD2 to the TGF-beta receptor and is disrupted by phosphorylation of SMAD2 upon TGF-beta receptor activation.
Interacts with SMAD3.
Interacts with TGFBR1 and TGFBR2; the interaction recruits SMAD2 to the TGF-beta receptor.
Interacts with PML.
4 PublicationsBinary interactionsi
O95405
GO - Molecular functioni
- protein domain specific binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 114773, 74 interactors |
CORUMi | O95405 |
IntActi | O95405, 42 interactors |
MINTi | O95405 |
STRINGi | 9606.ENSP00000287727 |
Miscellaneous databases
RNActi | O95405, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O95405 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O95405 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 201 – 255 | DisorderedSequence analysisAdd BLAST | 55 | |
Regioni | 291 – 352 | DisorderedSequence analysisAdd BLAST | 62 | |
Regioni | 767 – 823 | SBDAdd BLAST | 57 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 204 – 218 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 226 – 246 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 299 – 313 | Polar residuesSequence analysisAdd BLAST | 15 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 699 – 758 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 60 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1841, Eukaryota |
GeneTreei | ENSGT00940000154290 |
HOGENOMi | CLU_004326_1_0_1 |
InParanoidi | O95405 |
OMAi | HNLCSQV |
OrthoDBi | 278124at2759 |
PhylomeDBi | O95405 |
TreeFami | TF324904 |
Family and domain databases
DisProti | DP00141 |
Gene3Di | 3.30.40.10, 1 hit 4.10.720.10, 1 hit |
IDEALi | IID00112 |
InterProi | View protein in InterPro IPR022557, DUF3480 IPR035438, SARA/endofin IPR024608, SARA_Smad-bd IPR037145, SARA_Smad-bd_sf IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF11979, DUF3480, 1 hit PF01363, FYVE, 1 hit PF11409, SARA, 1 hit |
PIRSFi | PIRSF037289, SARA/endofin, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit |
SUPFAMi | SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50178, ZF_FYVE, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MENYFQAEAY NLDKVLDEFE QNEDETVSST LLDTKWNKIL DPPSHRLSFN
60 70 80 90 100
PTLASVNESA VSNESQPQLK VFSLAHSAPL TTEEEDHCAN GQDCNLNPEI
110 120 130 140 150
ATMWIDENAV AEDQLIKRNY SWDDQCSAVE VGEKKCGNLA CLPDEKNVLV
160 170 180 190 200
VAVMHNCDKR TLQNDLQDCN NYNSQSLMDA FSCSLDNENR QTDQFSFSIN
210 220 230 240 250
ESTEKDMNSE KQMDPLNRPK TEGRSVNHLC PTSSDSLASV CSPSQLKDDG
260 270 280 290 300
SIGRDPSMSA ITSLTVDSVI SSQGTDGCPA VKKQENYIPD EDLTGKISSP
310 320 330 340 350
RTDLGSPNSF SHMSEGILMK KEPAEESTTE ESLRSGLPLL LKPDMPNGSG
360 370 380 390 400
RNNDCERCSD CLVPNEVRAD ENEGYEHEET LGTTEFLNMT EHFSESQDMT
410 420 430 440 450
NWKLTKLNEM NDSQVNEEKE KFLQISQPED TNGDSGGQCV GLADAGLDLK
460 470 480 490 500
GTCISESEEC DFSTVIDTPA ANYLSNGCDS YGMQDPGVSF VPKTLPSKED
510 520 530 540 550
SVTEEKEIEE SKSECYSNIY EQRGNEATEG SGLLLNSTGD LMKKNYLHNF
560 570 580 590 600
CSQVPSVLGQ SSPKVVASLP SISVPFGGAR PKQPSNLKLQ IPKPLSDHLQ
610 620 630 640 650
NDFPANSGNN TKNKNDILGK AKLGENSATN VCSPSLGNIS NVDTNGEHLE
660 670 680 690 700
SYEAEISTRP CLALAPDSPD NDLRAGQFGI SARKPFTTLG EVAPVWVPDS
710 720 730 740 750
QAPNCMKCEA RFTFTKRRHH CRACGKVFCA SCCSLKCKLL YMDRKEARVC
760 770 780 790 800
VICHSVLMNA QAWENMMSAS SQSPNPNNPA EYCSTIPPLQ QAQASGALSS
810 820 830 840 850
PPPTVMVPVG VLKHPGAEVA QPREQRRVWF ADGILPNGEV ADAAKLTMNG
860 870 880 890 900
TSSAGTLAVS HDPVKPVTTS PLPAETDICL FSGSITQVGS PVGSAMNLIP
910 920 930 940 950
EDGLPPILIS TGVKGDYAVE EKPSQISVMQ QLEDGGPDPL VFVLNANLLS
960 970 980 990 1000
MVKIVNYVNR KCWCFTTKGM HAVGQSEIVI LLQCLPDEKC LPKDIFNHFV
1010 1020 1030 1040 1050
QLYRDALAGN VVSNLGHSFF SQSFLGSKEH GGFLYVTSTY QSLQDLVLPT
1060 1070 1080 1090 1100
PPYLFGILIQ KWETPWAKVF PIRLMLRLGA EYRLYPCPLF SVRFRKPLFG
1110 1120 1130 1140 1150
ETGHTIMNLL ADFRNYQYTL PVVQGLVVDM EVRKTSIKIP SNRYNEMMKA
1160 1170 1180 1190 1200
MNKSNEHVLA GGACFNEKAD SHLVCVQNDD GNYQTQAISI HNQPRKVTGA
1210 1220 1230 1240 1250
SFFVFSGALK SSSGYLAKSS IVEDGVMVQI TAENMDSLRQ ALREMKDFTI
1260 1270 1280 1290 1300
TCGKADAEEP QEHIHIQWVD DDKNVSKGVV SPIDGKSMET ITNVKIFHGS
1310 1320 1330 1340 1350
EYKANGKVIR WTEVFFLEND DQHNCLSDPA DHSRLTEHVA KAFCLALCPH
1360 1370 1380 1390 1400
LKLLKEDGMT KLGLRVTLDS DQVGYQAGSN GQPLPSQYMN DLDSALVPVI
1410 1420
HGGACQLSEG PVVMELIFYI LENIV
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052985 | 287 | Y → C. Corresponds to variant dbSNP:rs9803965Ensembl. | 1 | |
Natural variantiVAR_052986 | 414 | Q → P. Corresponds to variant dbSNP:rs3790525Ensembl. | 1 | |
Natural variantiVAR_052987 | 639 | I → V. Corresponds to variant dbSNP:rs11809887Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004315 | 760 – 818 | Missing in isoform 2. 1 PublicationAdd BLAST | 59 | |
Alternative sequenceiVSP_004316 | 760 – 762 | AQA → GKY in isoform 3. 1 Publication | 3 | |
Alternative sequenceiVSP_004317 | 763 – 1425 | Missing in isoform 3. 1 PublicationAdd BLAST | 663 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104304 mRNA Translation: AAC99462.1 Different initiation. AF130419 mRNA Translation: AAD31694.1 Frameshift. AF130420 mRNA Translation: AAD31695.1 AC105754 Genomic DNA No translation available. AL513218 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06790.1 CH471059 Genomic DNA Translation: EAX06791.1 BC032680 mRNA Translation: AAH32680.1 |
CCDSi | CCDS563.1 [O95405-1] CCDS564.1 [O95405-2] |
RefSeqi | NP_004790.2, NM_004799.3 [O95405-1] NP_015563.2, NM_007324.3 [O95405-2] XP_011540739.1, XM_011542437.2 [O95405-1] |
Genome annotation databases
Ensembli | ENST00000287727; ENSP00000287727; ENSG00000157077 ENST00000357206; ENSP00000349737; ENSG00000157077 [O95405-2] ENST00000371591; ENSP00000360647; ENSG00000157077 |
GeneIDi | 9372 |
KEGGi | hsa:9372 |
MANE-Selecti | ENST00000287727.8; ENSP00000287727.3; NM_004799.4; NP_004790.2 |
UCSCi | uc001cto.5, human [O95405-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104304 mRNA Translation: AAC99462.1 Different initiation. AF130419 mRNA Translation: AAD31694.1 Frameshift. AF130420 mRNA Translation: AAD31695.1 AC105754 Genomic DNA No translation available. AL513218 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06790.1 CH471059 Genomic DNA Translation: EAX06791.1 BC032680 mRNA Translation: AAH32680.1 |
CCDSi | CCDS563.1 [O95405-1] CCDS564.1 [O95405-2] |
RefSeqi | NP_004790.2, NM_004799.3 [O95405-1] NP_015563.2, NM_007324.3 [O95405-2] XP_011540739.1, XM_011542437.2 [O95405-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DEV | X-ray | 2.20 | B/D | 771-811 | [»] | |
1MK2 | X-ray | 2.74 | B | 773-810 | [»] | |
4BKW | X-ray | 2.53 | A | 895-1425 | [»] | |
5MJY | X-ray | 2.25 | E/F | 1-22 | [»] | |
SMRi | O95405 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114773, 74 interactors |
CORUMi | O95405 |
IntActi | O95405, 42 interactors |
MINTi | O95405 |
STRINGi | 9606.ENSP00000287727 |
PTM databases
GlyGeni | O95405, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O95405 |
PhosphoSitePlusi | O95405 |
Genetic variation databases
BioMutai | ZFYVE9 |
Proteomic databases
EPDi | O95405 |
jPOSTi | O95405 |
MassIVEi | O95405 |
MaxQBi | O95405 |
PaxDbi | O95405 |
PeptideAtlasi | O95405 |
PRIDEi | O95405 |
ProteomicsDBi | 50856 [O95405-1] 50857 [O95405-2] 50858 [O95405-3] |
Protocols and materials databases
Antibodypediai | 32995, 115 antibodies from 25 providers |
DNASUi | 9372 |
Genome annotation databases
Ensembli | ENST00000287727; ENSP00000287727; ENSG00000157077 ENST00000357206; ENSP00000349737; ENSG00000157077 [O95405-2] ENST00000371591; ENSP00000360647; ENSG00000157077 |
GeneIDi | 9372 |
KEGGi | hsa:9372 |
MANE-Selecti | ENST00000287727.8; ENSP00000287727.3; NM_004799.4; NP_004790.2 |
UCSCi | uc001cto.5, human [O95405-1] |
Organism-specific databases
CTDi | 9372 |
DisGeNETi | 9372 |
GeneCardsi | ZFYVE9 |
HGNCi | HGNC:6775, ZFYVE9 |
HPAi | ENSG00000157077, Low tissue specificity |
MIMi | 603755, gene |
neXtProti | NX_O95405 |
OpenTargetsi | ENSG00000157077 |
PharmGKBi | PA30532 |
VEuPathDBi | HostDB:ENSG00000157077 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1841, Eukaryota |
GeneTreei | ENSGT00940000154290 |
HOGENOMi | CLU_004326_1_0_1 |
InParanoidi | O95405 |
OMAi | HNLCSQV |
OrthoDBi | 278124at2759 |
PhylomeDBi | O95405 |
TreeFami | TF324904 |
Enzyme and pathway databases
PathwayCommonsi | O95405 |
Reactomei | R-HSA-2173788, Downregulation of TGF-beta receptor signaling [O95405-1] R-HSA-2173789, TGF-beta receptor signaling activates SMADs [O95405-1] R-HSA-3304356, SMAD2/3 Phosphorylation Motif Mutants in Cancer [O95405-1] R-HSA-3656532, TGFBR1 KD Mutants in Cancer [O95405-1] |
SignaLinki | O95405 |
SIGNORi | O95405 |
Miscellaneous databases
BioGRID-ORCSi | 9372, 8 hits in 1052 CRISPR screens |
ChiTaRSi | ZFYVE9, human |
EvolutionaryTracei | O95405 |
GeneWikii | Zinc_finger_FYVE_domain-containing_protein_9 |
GenomeRNAii | 9372 |
Pharosi | O95405, Tbio |
PROi | PR:O95405 |
RNActi | O95405, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000157077, Expressed in frontal cortex and 205 other tissues |
Genevisiblei | O95405, HS |
Family and domain databases
DisProti | DP00141 |
Gene3Di | 3.30.40.10, 1 hit 4.10.720.10, 1 hit |
IDEALi | IID00112 |
InterProi | View protein in InterPro IPR022557, DUF3480 IPR035438, SARA/endofin IPR024608, SARA_Smad-bd IPR037145, SARA_Smad-bd_sf IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF11979, DUF3480, 1 hit PF01363, FYVE, 1 hit PF11409, SARA, 1 hit |
PIRSFi | PIRSF037289, SARA/endofin, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit |
SUPFAMi | SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50178, ZF_FYVE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZFYV9_HUMAN | |
Accessioni | O95405Primary (citable) accession number: O95405 Secondary accession number(s): Q5T0F6 Q9Y5R7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 14, 2001 |
Last sequence update: | August 14, 2001 | |
Last modified: | February 23, 2022 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references