UniProtKB - O95402 (MED26_HUMAN)
Mediator of RNA polymerase II transcription subunit 26
MED26
Functioni
GO - Molecular functioni
- transcription coactivator activity Source: ProtInc
- transcription coregulator activity Source: UniProtKB
GO - Biological processi
- positive regulation of gene expression Source: GO_Central
- regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription initiation from RNA polymerase II promoter Source: Reactome
- transcription initiation from RNA polymerase II promoter Source: ProtInc
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | O95402 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-212436, Generic Transcription Pathway R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 26Alternative name(s): Activator-recruited cofactor 70 kDa component Short name: ARC70 Cofactor required for Sp1 transcriptional activation subunit 7 Short name: CRSP complex subunit 7 Mediator complex subunit 26 Transcriptional coactivator CRSP70 |
Gene namesi | Name:MED26 Synonyms:ARC70, CRSP7 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2376, MED26 |
MIMi | 605043, gene |
neXtProti | NX_O95402 |
VEuPathDBi | HostDB:ENSG00000105085.10 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- core mediator complex Source: GO_Central
- mediator complex Source: UniProtKB
- nucleoplasm Source: Reactome
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 9441 |
OpenTargetsi | ENSG00000105085 |
PharmGKBi | PA162395623 |
Miscellaneous databases
Pharosi | O95402, Tbio |
Genetic variation databases
BioMutai | MED26 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079360 | 1 – 600 | Mediator of RNA polymerase II transcription subunit 26Add BLAST | 600 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 447 | PhosphoserineCombined sources | 1 | |
Modified residuei | 470 | PhosphoserineCombined sources | 1 | |
Modified residuei | 535 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O95402 |
jPOSTi | O95402 |
MassIVEi | O95402 |
MaxQBi | O95402 |
PaxDbi | O95402 |
PeptideAtlasi | O95402 |
PRIDEi | O95402 |
ProteomicsDBi | 50854 [O95402-1] 50855 [O95402-2] |
TopDownProteomicsi | O95402-1 [O95402-1] |
PTM databases
iPTMneti | O95402 |
PhosphoSitePlusi | O95402 |
Expressioni
Gene expression databases
Bgeei | ENSG00000105085, Expressed in sperm and 225 other tissues |
ExpressionAtlasi | O95402, baseline and differential |
Genevisiblei | O95402, HS |
Organism-specific databases
HPAi | ENSG00000105085, Tissue enhanced (testis) |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
Interacts with CEBPB (when not methylated)(PubMed:20111005).
4 PublicationsBinary interactionsi
Hide detailsO95402
With | #Exp. | IntAct |
---|---|---|
EAF1 [Q96JC9] | 5 | EBI-394392,EBI-769261 |
MED4 [Q9NPJ6] | 9 | EBI-394392,EBI-394607 |
MED7 [O43513] | 8 | EBI-394392,EBI-394632 |
Med19 [Q8C1S0] from Mus musculus. | 2 | EBI-394392,EBI-398761 |
Protein-protein interaction databases
BioGRIDi | 114831, 91 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | O95402 |
DIPi | DIP-31464N |
IntActi | O95402, 96 interactors |
STRINGi | 9606.ENSP00000263390 |
Miscellaneous databases
RNActi | O95402, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O95402 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 87 | TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST | 78 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 228 – 325 | Pro-richAdd BLAST | 98 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1105, Eukaryota |
GeneTreei | ENSGT00390000000259 |
HOGENOMi | CLU_478915_0_0_1 |
InParanoidi | O95402 |
OMAi | GCKAGLP |
OrthoDBi | 459216at2759 |
PhylomeDBi | O95402 |
TreeFami | TF328436 |
Family and domain databases
Gene3Di | 1.20.930.10, 1 hit |
InterProi | View protein in InterPro IPR042376, MED26 IPR031416, Med26_C IPR031417, Med26_Mid IPR003617, TFIIS/CRSP70_N_sub IPR035441, TFIIS/LEDGF_dom_sf IPR017923, TFIIS_N |
PANTHERi | PTHR15201, PTHR15201, 1 hit |
Pfami | View protein in Pfam PF08711, Med26, 1 hit PF15693, Med26_C, 1 hit PF15694, Med26_M, 1 hit |
SMARTi | View protein in SMART SM00509, TFS2N, 1 hit |
SUPFAMi | SSF47676, SSF47676, 1 hit |
PROSITEi | View protein in PROSITE PS51319, TFIIS_N, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTAAPASPQQ IRDRLLQAID PQSNIRNMVA VLEVISSLEK YPITKEALEE
60 70 80 90 100
TRLGKLINDV RKKTKNEELA KRAKKLLRSW QKLIEPAHQH EAALRGLAGA
110 120 130 140 150
TGSANGGAHN CRPEVGAAGP PRSIHDLKSR NDLQRLPGQR LDRLGSRKRR
160 170 180 190 200
GDQRDLGHPG PPPKVSKASH DPLVPNSSPL PTNGISGSPE SFASSLDGSG
210 220 230 240 250
HAGPEGSRLE RDENDKHSGK IPVNAVRPHT SSPGLGKPPG PCLQPKASVL
260 270 280 290 300
QQLDRVDETP GPPHPKGPPR CSFSPRNSRH EGSFARQQSL YAPKGSVPSP
310 320 330 340 350
SPRPQALDAT QVPSPLPLAQ PSTPPVRRLE LLPSAESPVC WLEQPESHQR
360 370 380 390 400
LAGPGCKAGL SPAEPLLSRA GFSPDSSKAD SDAASSGGSD SKKKKRYRPR
410 420 430 440 450
DYTVNLDGQV AEAGVKPVRL KERKLTFDPM TRQIKPLTQK EPVRADSPVH
460 470 480 490 500
MEQQSRTELD KQEAKASLQS PFEQTNWKEL SRNEIIQSYL SRQSSLLSSS
510 520 530 540 550
GAQTPGAHHF MSEYLKQEES TRQGARQLHV LVPQSPPTDL PGLTREVTQD
560 570 580 590 600
DLDRIQASQW PGVNGCQDTQ GNWYDWTQCI SLDPHGDDGR LNILPYVCLD
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0R064 | M0R064_HUMAN | Mediator complex subunit 26 | MED26 | 213 | Annotation score: | ||
M0QXW8 | M0QXW8_HUMAN | Mediator complex subunit 26 | MED26 | 110 | Annotation score: | ||
M0R2P1 | M0R2P1_HUMAN | Mediator complex subunit 26 | MED26 | 54 | Annotation score: | ||
M0R2Y4 | M0R2Y4_HUMAN | Mediator of RNA polymerase II trans... | MED26 | 82 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 156 | L → F in AAD12722 (PubMed:9989412).Curated | 1 | |
Sequence conflicti | 241 | P → A in AAI27216 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 369 | R → L in AAI10431 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028151 | 76 – 92 | LLRSW…HQHEA → LPGWQWACRPRGQPPGA in isoform 2. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_028152 | 93 – 600 | Missing in isoform 2. 1 PublicationAdd BLAST | 508 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104253 mRNA Translation: AAD12722.1 BC110430 mRNA Translation: AAI10431.1 BC127215 mRNA Translation: AAI27216.1 |
CCDSi | CCDS12347.1 [O95402-1] |
RefSeqi | NP_004822.2, NM_004831.3 [O95402-1] |
Genome annotation databases
Ensembli | ENST00000263390; ENSP00000263390; ENSG00000105085 [O95402-1] ENST00000611692; ENSP00000484490; ENSG00000105085 [O95402-2] |
GeneIDi | 9441 |
KEGGi | hsa:9441 |
UCSCi | uc002nen.2, human [O95402-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104253 mRNA Translation: AAD12722.1 BC110430 mRNA Translation: AAI10431.1 BC127215 mRNA Translation: AAI27216.1 |
CCDSi | CCDS12347.1 [O95402-1] |
RefSeqi | NP_004822.2, NM_004831.3 [O95402-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ODD | NMR | - | A | 1-92 | [»] | |
SMRi | O95402 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114831, 91 interactors |
ComplexPortali | CPX-3227, Core mediator complex |
CORUMi | O95402 |
DIPi | DIP-31464N |
IntActi | O95402, 96 interactors |
STRINGi | 9606.ENSP00000263390 |
PTM databases
iPTMneti | O95402 |
PhosphoSitePlusi | O95402 |
Genetic variation databases
BioMutai | MED26 |
Proteomic databases
EPDi | O95402 |
jPOSTi | O95402 |
MassIVEi | O95402 |
MaxQBi | O95402 |
PaxDbi | O95402 |
PeptideAtlasi | O95402 |
PRIDEi | O95402 |
ProteomicsDBi | 50854 [O95402-1] 50855 [O95402-2] |
TopDownProteomicsi | O95402-1 [O95402-1] |
Protocols and materials databases
Antibodypediai | 14206, 278 antibodies |
Genome annotation databases
Ensembli | ENST00000263390; ENSP00000263390; ENSG00000105085 [O95402-1] ENST00000611692; ENSP00000484490; ENSG00000105085 [O95402-2] |
GeneIDi | 9441 |
KEGGi | hsa:9441 |
UCSCi | uc002nen.2, human [O95402-1] |
Organism-specific databases
CTDi | 9441 |
DisGeNETi | 9441 |
GeneCardsi | MED26 |
HGNCi | HGNC:2376, MED26 |
HPAi | ENSG00000105085, Tissue enhanced (testis) |
MIMi | 605043, gene |
neXtProti | NX_O95402 |
OpenTargetsi | ENSG00000105085 |
PharmGKBi | PA162395623 |
VEuPathDBi | HostDB:ENSG00000105085.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1105, Eukaryota |
GeneTreei | ENSGT00390000000259 |
HOGENOMi | CLU_478915_0_0_1 |
InParanoidi | O95402 |
OMAi | GCKAGLP |
OrthoDBi | 459216at2759 |
PhylomeDBi | O95402 |
TreeFami | TF328436 |
Enzyme and pathway databases
PathwayCommonsi | O95402 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-212436, Generic Transcription Pathway R-HSA-381340, Transcriptional regulation of white adipocyte differentiation |
Miscellaneous databases
BioGRID-ORCSi | 9441, 587 hits in 990 CRISPR screens |
ChiTaRSi | MED26, human |
GeneWikii | MED26 |
GenomeRNAii | 9441 |
Pharosi | O95402, Tbio |
PROi | PR:O95402 |
RNActi | O95402, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000105085, Expressed in sperm and 225 other tissues |
ExpressionAtlasi | O95402, baseline and differential |
Genevisiblei | O95402, HS |
Family and domain databases
Gene3Di | 1.20.930.10, 1 hit |
InterProi | View protein in InterPro IPR042376, MED26 IPR031416, Med26_C IPR031417, Med26_Mid IPR003617, TFIIS/CRSP70_N_sub IPR035441, TFIIS/LEDGF_dom_sf IPR017923, TFIIS_N |
PANTHERi | PTHR15201, PTHR15201, 1 hit |
Pfami | View protein in Pfam PF08711, Med26, 1 hit PF15693, Med26_C, 1 hit PF15694, Med26_M, 1 hit |
SMARTi | View protein in SMART SM00509, TFS2N, 1 hit |
SUPFAMi | SSF47676, SSF47676, 1 hit |
PROSITEi | View protein in PROSITE PS51319, TFIIS_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MED26_HUMAN | |
Accessioni | O95402Primary (citable) accession number: O95402 Secondary accession number(s): A1A4S3, Q0VGB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2003 |
Last sequence update: | October 2, 2007 | |
Last modified: | April 7, 2021 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families