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Protein

Tripartite motif-containing protein 16

Gene

TRIM16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.3 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 122B box-type 1PROSITE-ProRule annotation1 PublicationAdd BLAST51
Zinc fingeri126 – 165B box-type 2PROSITE-ProRule annotation1 PublicationAdd BLAST40

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • interleukin-1 binding Source: UniProtKB
  • NACHT domain binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 16 (EC:2.3.2.271 Publication)
Alternative name(s):
E3 ubiquitin-protein ligase TRIM16
Estrogen-responsive B box protein
Gene namesi
Name:TRIM16
Synonyms:EBBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000221926.11
HGNCiHGNC:17241 TRIM16
MIMi609505 gene
neXtProtiNX_O95361

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116S → A: Loss of protection from cell death during oxidative stress; when associated with A-203. 1 Publication1
Mutagenesisi203S → A: Loss of protection from cell death during oxidative stress; when associated with A-116. 1 Publication1

Organism-specific databases

DisGeNETi10626
OpenTargetsiENSG00000221926
PharmGKBiPA38215

Polymorphism and mutation databases

BioMutaiTRIM16

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562221 – 564Tripartite motif-containing protein 16Add BLAST564

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116Phosphoserine1 Publication1
Modified residuei203Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated by ULK1.1 Publication
Auto-ubiquitinates via its B-Boxes.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO95361
MaxQBiO95361
PaxDbiO95361
PeptideAtlasiO95361
PRIDEiO95361
ProteomicsDBi50822
50823 [O95361-2]

PTM databases

iPTMnetiO95361
PhosphoSitePlusiO95361

Expressioni

Tissue specificityi

Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.

Gene expression databases

BgeeiENSG00000221926 Expressed in 92 organ(s), highest expression level in ectocervix
CleanExiHS_TRIM16
ExpressionAtlasiO95361 baseline and differential
GenevisibleiO95361 HS

Organism-specific databases

HPAiHPA023623
HPA066431

Interactioni

Subunit structurei

Homodimerizes via its coiled-coil domain. Heterodimerizes with MID1, TRIM24 and PML. Interacts with Galectin-3/LGALS3 in a ULK1-dependent manner; this interaction mediates autophagy of damage endomembranes. Interacts with BECN1. Interacts with ATG16L1. Interacts with p62/SQSTM and LC3B/MAP1LC3B.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115870, 30 interactors
IntActiO95361, 11 interactors
STRINGi9606.ENSP00000338989

Structurei

3D structure databases

ProteinModelPortaliO95361
SMRiO95361
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini355 – 553B30.2/SPRYPROSITE-ProRule annotationAdd BLAST199

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili165 – 203Sequence analysisAdd BLAST39
Coiled coili243 – 274Sequence analysisAdd BLAST32
Coiled coili320 – 340Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 122B box-type 1PROSITE-ProRule annotation1 PublicationAdd BLAST51
Zinc fingeri126 – 165B box-type 2PROSITE-ProRule annotation1 PublicationAdd BLAST40

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410II63 Eukaryota
ENOG4110QZ1 LUCA
GeneTreeiENSGT00760000118995
HOGENOMiHOG000060240
HOVERGENiHBG057134
InParanoidiO95361
KOiK12006
OMAiWHSDVET
OrthoDBiEOG091G03PP
PhylomeDBiO95361
TreeFamiTF351086

Family and domain databases

InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR003877 SPRY_dom
IPR000315 Znf_B-box
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 2 hits
SM00589 PRY, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O95361-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAELDLMAPG PLPRATAQPP APLSPDSGSP SPDSGSASPV EEEDVGSSEK
60 70 80 90 100
LGRETEEQDS DSAEQGDPAG EGKEVLCDFC LDDTRRVKAV KSCLTCMVNY
110 120 130 140 150
CEEHLQPHQV NIKLQSHLLT EPVKDHNWRY CPAHHSPLSA FCCPDQQCIC
160 170 180 190 200
QDCCQEHSGH TIVSLDAARR DKEAELQCTQ LDLERKLKLN ENAISRLQAN
210 220 230 240 250
QKSVLVSVSE VKAVAEMQFG ELLAAVRKAQ ANVMLFLEEK EQAALSQANG
260 270 280 290 300
IKAHLEYRSA EMEKSKQELE RMAAISNTVQ FLEEYCKFKN TEDITFPSVY
310 320 330 340 350
VGLKDKLSGI RKVITESTVH LIQLLENYKK KLQEFSKEEE YDIRTQVSAV
360 370 380 390 400
VQRKYWTSKP EPSTREQFLQ YAYDITFDPD TAHKYLRLQE ENRKVTNTTP
410 420 430 440 450
WEHPYPDLPS RFLHWRQVLS QQSLYLHRYY FEVEIFGAGT YVGLTCKGID
460 470 480 490 500
RKGEERNSCI SGNNFSWSLQ WNGKEFTAWY SDMETPLKAG PFRRLGVYID
510 520 530 540 550
FPGGILSFYG VEYDTMTLVH KFACKFSEPV YAAFWLSKKE NAIRIVDLGE
560
EPEKPAPSLV GTAP
Length:564
Mass (Da):63,955
Last modified:April 17, 2007 - v3
Checksum:iA6760717937D82F8
GO
Isoform 2 (identifier: O95361-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-216: Missing.

Show »
Length:348
Mass (Da):40,370
Checksum:i6C2EC2F9E97FEB5D
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B3KP96B3KP96_HUMAN
cDNA FLJ31464 fis, clone NT2NE20013...
TRIM16
434Annotation score:
K7ENN8K7ENN8_HUMAN
Tripartite motif-containing protein...
TRIM16
232Annotation score:
K7EJH2K7EJH2_HUMAN
Tripartite motif-containing protein...
TRIM16
55Annotation score:
K7EL43K7EL43_HUMAN
Tripartite motif-containing protein...
TRIM16
184Annotation score:
J3QLP0J3QLP0_HUMAN
Tripartite motif-containing protein...
TRIM16
140Annotation score:
J3QKY5J3QKY5_HUMAN
Tripartite motif-containing protein...
TRIM16
115Annotation score:
J3QR69J3QR69_HUMAN
Tripartite motif-containing protein...
TRIM16
111Annotation score:
J3QL38J3QL38_HUMAN
Tripartite motif-containing protein...
TRIM16
96Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235L → P in AAC79080 (PubMed:9817599).Curated1
Sequence conflicti235L → P (PubMed:11919186).Curated1
Sequence conflicti246S → N in AAC79080 (PubMed:9817599).Curated1
Sequence conflicti246S → N (PubMed:11919186).Curated1
Sequence conflicti465F → L in AAH01564 (PubMed:15489334).Curated1
Sequence conflicti521K → N in AAC79080 (PubMed:9817599).Curated1
Sequence conflicti521K → N (PubMed:11919186).Curated1
Sequence conflicti541N → S in AAC79080 (PubMed:9817599).Curated1
Sequence conflicti541N → S (PubMed:11919186).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017412121E → D. Corresponds to variant dbSNP:rs2074890Ensembl.1
Natural variantiVAR_052133493R → W. Corresponds to variant dbSNP:rs3174720Ensembl.1
Natural variantiVAR_031668561G → V4 PublicationsCorresponds to variant dbSNP:rs1060903Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0090981 – 216Missing in isoform 2. 1 PublicationAdd BLAST216

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096870 mRNA Translation: AAC79080.1
CR457136 mRNA Translation: CAG33417.1
BC001564 mRNA Translation: AAH01564.1
BC053514 mRNA Translation: AAH53514.1
BC067096 mRNA Translation: AAH67096.1
CCDSiCCDS11171.1 [O95361-1]
CCDS86578.1 [O95361-2]
RefSeqiNP_001335048.1, NM_001348119.1 [O95361-1]
NP_001335049.1, NM_001348120.1 [O95361-1]
NP_001335053.1, NM_001348124.1 [O95361-2]
NP_001335054.1, NM_001348125.1 [O95361-2]
NP_006461.3, NM_006470.4 [O95361-1]
UniGeneiHs.123534

Genome annotation databases

EnsembliENST00000336708; ENSP00000338989; ENSG00000221926 [O95361-1]
ENST00000577886; ENSP00000462903; ENSG00000221926 [O95361-2]
ENST00000578237; ENSP00000463188; ENSG00000221926 [O95361-1]
GeneIDi10626
KEGGihsa:10626
UCSCiuc002gow.4 human [O95361-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096870 mRNA Translation: AAC79080.1
CR457136 mRNA Translation: CAG33417.1
BC001564 mRNA Translation: AAH01564.1
BC053514 mRNA Translation: AAH53514.1
BC067096 mRNA Translation: AAH67096.1
CCDSiCCDS11171.1 [O95361-1]
CCDS86578.1 [O95361-2]
RefSeqiNP_001335048.1, NM_001348119.1 [O95361-1]
NP_001335049.1, NM_001348120.1 [O95361-1]
NP_001335053.1, NM_001348124.1 [O95361-2]
NP_001335054.1, NM_001348125.1 [O95361-2]
NP_006461.3, NM_006470.4 [O95361-1]
UniGeneiHs.123534

3D structure databases

ProteinModelPortaliO95361
SMRiO95361
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115870, 30 interactors
IntActiO95361, 11 interactors
STRINGi9606.ENSP00000338989

PTM databases

iPTMnetiO95361
PhosphoSitePlusiO95361

Polymorphism and mutation databases

BioMutaiTRIM16

Proteomic databases

EPDiO95361
MaxQBiO95361
PaxDbiO95361
PeptideAtlasiO95361
PRIDEiO95361
ProteomicsDBi50822
50823 [O95361-2]

Protocols and materials databases

DNASUi10626
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336708; ENSP00000338989; ENSG00000221926 [O95361-1]
ENST00000577886; ENSP00000462903; ENSG00000221926 [O95361-2]
ENST00000578237; ENSP00000463188; ENSG00000221926 [O95361-1]
GeneIDi10626
KEGGihsa:10626
UCSCiuc002gow.4 human [O95361-1]

Organism-specific databases

CTDi10626
DisGeNETi10626
EuPathDBiHostDB:ENSG00000221926.11
GeneCardsiTRIM16
H-InvDBiHIX0013608
HGNCiHGNC:17241 TRIM16
HPAiHPA023623
HPA066431
MIMi609505 gene
neXtProtiNX_O95361
OpenTargetsiENSG00000221926
PharmGKBiPA38215
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410II63 Eukaryota
ENOG4110QZ1 LUCA
GeneTreeiENSGT00760000118995
HOGENOMiHOG000060240
HOVERGENiHBG057134
InParanoidiO95361
KOiK12006
OMAiWHSDVET
OrthoDBiEOG091G03PP
PhylomeDBiO95361
TreeFamiTF351086

Miscellaneous databases

ChiTaRSiTRIM16 human
GeneWikiiTRIM16
GenomeRNAii10626
PROiPR:O95361
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000221926 Expressed in 92 organ(s), highest expression level in ectocervix
CleanExiHS_TRIM16
ExpressionAtlasiO95361 baseline and differential
GenevisibleiO95361 HS

Family and domain databases

InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR003877 SPRY_dom
IPR000315 Znf_B-box
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 2 hits
SM00589 PRY, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRI16_HUMAN
AccessioniPrimary (citable) accession number: O95361
Secondary accession number(s): Q6IAL8
, Q7Z6I2, Q96BE8, Q96J43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: April 17, 2007
Last modified: November 7, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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