UniProtKB - O95352 (ATG7_HUMAN)
Ubiquitin-like modifier-activating enzyme ATG7
ATG7
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 572 | Glycyl thioester intermediateBy similarity | 1 |
GO - Molecular functioni
- Atg12 activating enzyme activity Source: UniProtKB
- Atg8 activating enzyme activity Source: GO_Central
- protein homodimerization activity Source: UniProtKB
- transcription factor binding Source: UniProtKB
GO - Biological processi
- aging Source: Ensembl
- autophagosome assembly Source: GO_Central
- autophagy Source: GO_Central
- autophagy of mitochondrion Source: ParkinsonsUK-UCL
- cellular response to hyperoxia Source: UniProtKB
- cellular response to morphine Source: Ensembl
- cellular response to nitrogen starvation Source: GO_Central
- cellular response to starvation Source: UniProtKB
- chaperone-mediated autophagy Source: Ensembl
- C-terminal protein lipidation Source: GO_Central
- defense response to virus Source: CACAO
- late nucleophagy Source: GO_Central
- macroautophagy Source: BHF-UCL
- negative regulation of mitochondrial DNA replication Source: Ensembl
- negative regulation of oxidative stress-induced neuron death Source: Ensembl
- neutrophil degranulation Source: Reactome
- piecemeal microautophagy of the nucleus Source: GO_Central
- positive regulation by symbiont of host autophagy Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of autophagy Source: Ensembl
- positive regulation of protein catabolic process Source: UniProtKB
- positive regulation of protein modification process Source: MGI
- protein lipidation Source: MGI
- protein modification by small protein conjugation Source: GO_Central
- protein transport Source: UniProtKB-KW
- regulation of circadian rhythm Source: UniProtKB
- response to fluoride Source: Ensembl
- response to glucose Source: Ensembl
- rhythmic process Source: UniProtKB-KW
- suppression by virus of host autophagy Source: CACAO
Keywordsi
Biological process | Autophagy, Biological rhythms, Protein transport, Transport, Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | O95352 |
Reactomei | R-HSA-1632852, Macroautophagy R-HSA-6798695, Neutrophil degranulation R-HSA-6802952, Signaling by BRAF and RAF fusions R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SIGNORi | O95352 |
Protein family/group databases
TCDBi | 9.A.15.2.1, the autophagy-related phagophore-formation transporter (apt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-like modifier-activating enzyme ATG7Alternative name(s): ATG12-activating enzyme E1 ATG7 Autophagy-related protein 7 Short name: APG7-like Short name: hAGP7 Ubiquitin-activating enzyme E1-like protein |
Gene namesi | Name:ATG7 Synonyms:APG7L |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16935, ATG7 |
MIMi | 608760, gene |
neXtProti | NX_O95352 |
VEuPathDBi | HostDB:ENSG00000197548.12 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- Preautophagosomal structure By similarity
Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.By similarity
Cytoskeleton
- axoneme Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular region Source: Reactome
Other locations
- axon Source: Ensembl
- cytoplasm Source: UniProtKB
- ficolin-1-rich granule lumen Source: Reactome
- phagophore assembly site Source: GO_Central
- secretory granule lumen Source: Reactome
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 15 | F → D: Impairs conjugation activity; when associated with D-16 and D-17. 1 Publication | 1 | |
Mutagenesisi | 16 | A → D: Impairs conjugation activity; when associated with D-15 and D-17. 1 Publication | 1 | |
Mutagenesisi | 17 | P → D: Impairs conjugation activity; when associated with D-15 and D-16. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10533 |
OpenTargetsi | ENSG00000197548 |
PharmGKBi | PA134983397 |
Miscellaneous databases
Pharosi | O95352, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2321621 |
Genetic variation databases
BioMutai | ATG7 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000212806 | 2 – 703 | Ubiquitin-like modifier-activating enzyme ATG7Add BLAST | 702 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 698 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O95352 |
jPOSTi | O95352 |
MassIVEi | O95352 |
MaxQBi | O95352 |
PaxDbi | O95352 |
PeptideAtlasi | O95352 |
PRIDEi | O95352 |
ProteomicsDBi | 19175 50814 [O95352-1] 50815 [O95352-2] |
PTM databases
iPTMneti | O95352 |
MetOSitei | O95352 |
PhosphoSitePlusi | O95352 |
SwissPalmi | O95352 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000197548, Expressed in monocyte and 201 other tissues |
ExpressionAtlasi | O95352, baseline and differential |
Genevisiblei | O95352, HS |
Organism-specific databases
HPAi | ENSG00000197548, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer (PubMed:11096062).
Interacts with ATG3 and ATG12 (PubMed:11096062, PubMed:11825910). The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5 (PubMed:11825910). Forms intermediate conjugates with ATG8-like proteins such as GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A (PubMed:11096062, PubMed:16303767).
Interacts with EP300 acetyltransferase (PubMed:19124466).
Interacts with FOXO1 (PubMed:20543840).
5 PublicationsBinary interactionsi
Hide detailsO95352
Isoform 2 [O95352-2]
With | #Exp. | IntAct |
---|---|---|
TARDBP [Q13148] | 3 | EBI-15980880,EBI-372899 |
TP53 [P04637] | 4 | EBI-15980880,EBI-366083 |
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115787, 79 interactors |
DIPi | DIP-29759N |
IntActi | O95352, 33 interactors |
STRINGi | 9606.ENSP00000346437 |
Chemistry databases
BindingDBi | O95352 |
Miscellaneous databases
RNActi | O95352, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 15 – 17 | FAP motif | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2337, Eukaryota |
GeneTreei | ENSGT00390000017509 |
HOGENOMi | CLU_012998_1_0_1 |
InParanoidi | O95352 |
OMAi | PMAGHPI |
OrthoDBi | 549762at2759 |
PhylomeDBi | O95352 |
TreeFami | TF105689 |
Family and domain databases
Gene3Di | 3.40.140.100, 1 hit 3.40.140.70, 1 hit |
InterProi | View protein in InterPro IPR006285, Atg7 IPR032197, Atg7_N IPR042522, Atg7_N_1 IPR042523, Atg7_N_2 IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF16420, ATG7_N, 1 hit PF00899, ThiF, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
TIGRFAMsi | TIGR01381, E1_like_apg7, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 12 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY
60 70 80 90 100
YYNGDSAGLP ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD
110 120 130 140 150
KKLLLEQAAN EIWESIKSGT ALENPVLLNK FLLLTFADLK KYHFYYWFCY
160 170 180 190 200
PALCLPESLP LIQGPVGLDQ RFSLKQIEAL ECAYDNLCQT EGVTALPYFL
210 220 230 240 250
IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY PGWPLRNFLV
260 270 280 290 300
LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK
310 320 330 340 350
AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL
360 370 380 390 400
DKVVSVKCLL LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL
410 420 430 440 450
YEFEDCLGGG KPKALAAADR LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT
460 470 480 490 500
LEQARRDVEQ LEQLIESHDV VFLLMDTRES RWLPAVIAAS KRKLVINAAL
510 520 530 540 550
GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF ANIPGYKLGC
560 570 580 590 600
YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG
610 620 630 640 650
GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS
660 670 680 690 700
SKVLDQYERE GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD
ETI
Computationally mapped potential isoform sequencesi
There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J415 | C9J415_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 152 | Annotation score: | ||
C9JGL2 | C9JGL2_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 66 | Annotation score: | ||
C9JFF4 | C9JFF4_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 176 | Annotation score: | ||
F8WDY9 | F8WDY9_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 76 | Annotation score: | ||
H7C059 | H7C059_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 105 | Annotation score: | ||
H7C369 | H7C369_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 77 | Annotation score: | ||
C9JE55 | C9JE55_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 126 | Annotation score: | ||
C9JKA3 | C9JKA3_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 93 | Annotation score: | ||
C9JNU2 | C9JNU2_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 89 | Annotation score: | ||
H7C2J8 | H7C2J8_HUMAN | Ubiquitin-like modifier-activating ... | ATG7 | 139 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 210 | V → A in BAG64682 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 346 | P → L in BAG64682 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_053014 | 471 | V → A. Corresponds to variant dbSNP:rs36117895Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045206 | 138 – 176 | Missing in isoform 3. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_013205 | 626 – 652 | Missing in isoform 2. 1 PublicationAdd BLAST | 27 | |
Alternative sequenceiVSP_045207 | 653 – 693 | Missing in isoform 3. 1 PublicationAdd BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF094516 mRNA Translation: AAC69630.1 AK303694 mRNA Translation: BAG64682.1 AC020750 Genomic DNA No translation available. AC022001 Genomic DNA No translation available. AC026185 Genomic DNA No translation available. AC083855 Genomic DNA No translation available. BC000091 mRNA Translation: AAH00091.1 AL122075 mRNA Translation: CAB59250.1 |
CCDSi | CCDS2605.1 [O95352-1] CCDS46752.1 [O95352-2] CCDS46753.1 [O95352-3] |
PIRi | T34556 |
RefSeqi | NP_001129503.2, NM_001136031.2 [O95352-2] NP_001138384.1, NM_001144912.1 [O95352-3] NP_006386.1, NM_006395.2 [O95352-1] XP_011531584.1, XM_011533282.2 XP_011531588.1, XM_011533286.2 [O95352-2] XP_016861033.1, XM_017005544.1 XP_016861034.1, XM_017005545.1 XP_016861035.1, XM_017005546.1 |
Genome annotation databases
Ensembli | ENST00000354449; ENSP00000346437; ENSG00000197548 [O95352-1] ENST00000354956; ENSP00000347042; ENSG00000197548 [O95352-2] ENST00000446450; ENSP00000412580; ENSG00000197548 [O95352-3] |
GeneIDi | 10533 |
KEGGi | hsa:10533 |
UCSCi | uc003bwc.4, human [O95352-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF094516 mRNA Translation: AAC69630.1 AK303694 mRNA Translation: BAG64682.1 AC020750 Genomic DNA No translation available. AC022001 Genomic DNA No translation available. AC026185 Genomic DNA No translation available. AC083855 Genomic DNA No translation available. BC000091 mRNA Translation: AAH00091.1 AL122075 mRNA Translation: CAB59250.1 |
CCDSi | CCDS2605.1 [O95352-1] CCDS46752.1 [O95352-2] CCDS46753.1 [O95352-3] |
PIRi | T34556 |
RefSeqi | NP_001129503.2, NM_001136031.2 [O95352-2] NP_001138384.1, NM_001144912.1 [O95352-3] NP_006386.1, NM_006395.2 [O95352-1] XP_011531584.1, XM_011533282.2 XP_011531588.1, XM_011533286.2 [O95352-2] XP_016861033.1, XM_017005544.1 XP_016861034.1, XM_017005545.1 XP_016861035.1, XM_017005546.1 |
3D structure databases
SMRi | O95352 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115787, 79 interactors |
DIPi | DIP-29759N |
IntActi | O95352, 33 interactors |
STRINGi | 9606.ENSP00000346437 |
Chemistry databases
BindingDBi | O95352 |
ChEMBLi | CHEMBL2321621 |
Protein family/group databases
TCDBi | 9.A.15.2.1, the autophagy-related phagophore-formation transporter (apt) family |
PTM databases
iPTMneti | O95352 |
MetOSitei | O95352 |
PhosphoSitePlusi | O95352 |
SwissPalmi | O95352 |
Genetic variation databases
BioMutai | ATG7 |
Proteomic databases
EPDi | O95352 |
jPOSTi | O95352 |
MassIVEi | O95352 |
MaxQBi | O95352 |
PaxDbi | O95352 |
PeptideAtlasi | O95352 |
PRIDEi | O95352 |
ProteomicsDBi | 19175 50814 [O95352-1] 50815 [O95352-2] |
Protocols and materials databases
Antibodypediai | 1972, 832 antibodies |
Genome annotation databases
Ensembli | ENST00000354449; ENSP00000346437; ENSG00000197548 [O95352-1] ENST00000354956; ENSP00000347042; ENSG00000197548 [O95352-2] ENST00000446450; ENSP00000412580; ENSG00000197548 [O95352-3] |
GeneIDi | 10533 |
KEGGi | hsa:10533 |
UCSCi | uc003bwc.4, human [O95352-1] |
Organism-specific databases
CTDi | 10533 |
DisGeNETi | 10533 |
GeneCardsi | ATG7 |
HGNCi | HGNC:16935, ATG7 |
HPAi | ENSG00000197548, Low tissue specificity |
MIMi | 608760, gene |
neXtProti | NX_O95352 |
OpenTargetsi | ENSG00000197548 |
PharmGKBi | PA134983397 |
VEuPathDBi | HostDB:ENSG00000197548.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2337, Eukaryota |
GeneTreei | ENSGT00390000017509 |
HOGENOMi | CLU_012998_1_0_1 |
InParanoidi | O95352 |
OMAi | PMAGHPI |
OrthoDBi | 549762at2759 |
PhylomeDBi | O95352 |
TreeFami | TF105689 |
Enzyme and pathway databases
PathwayCommonsi | O95352 |
Reactomei | R-HSA-1632852, Macroautophagy R-HSA-6798695, Neutrophil degranulation R-HSA-6802952, Signaling by BRAF and RAF fusions R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SIGNORi | O95352 |
Miscellaneous databases
BioGRID-ORCSi | 10533, 21 hits in 1006 CRISPR screens |
ChiTaRSi | ATG7, human |
GeneWikii | ATG7 |
GenomeRNAii | 10533 |
Pharosi | O95352, Tbio |
PROi | PR:O95352 |
RNActi | O95352, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000197548, Expressed in monocyte and 201 other tissues |
ExpressionAtlasi | O95352, baseline and differential |
Genevisiblei | O95352, HS |
Family and domain databases
Gene3Di | 3.40.140.100, 1 hit 3.40.140.70, 1 hit |
InterProi | View protein in InterPro IPR006285, Atg7 IPR032197, Atg7_N IPR042522, Atg7_N_1 IPR042523, Atg7_N_2 IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF16420, ATG7_N, 1 hit PF00899, ThiF, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
TIGRFAMsi | TIGR01381, E1_like_apg7, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATG7_HUMAN | |
Accessioni | O95352Primary (citable) accession number: O95352 Secondary accession number(s): B4E170 Q9UFH4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | May 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families