UniProtKB - O95319 (CELF2_HUMAN)
Protein
CUGBP Elav-like family member 2
Gene
CELF2
Organism
Homo sapiens (Human)
Status
Functioni
RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity). May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA (PubMed:28431233).By similarity8 Publications
GO - Molecular functioni
- mRNA binding Source: GO_Central
- pre-mRNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA splice site selection Source: UniProtKB
- regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
- regulation of heart contraction Source: ProtInc
- RNA processing Source: ProtInc
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | mRNA processing |
Enzyme and pathway databases
PathwayCommonsi | O95319 |
SIGNORi | O95319 |
Names & Taxonomyi
Protein namesi | Recommended name: CUGBP Elav-like family member 2Short name: CELF-2 Alternative name(s): Bruno-like protein 3 CUG triplet repeat RNA-binding protein 2 Short name: CUG-BP2 CUG-BP- and ETR-3-like factor 2 ELAV-type RNA-binding protein 3 Short name: ETR-3 Neuroblastoma apoptosis-related RNA-binding protein Short name: hNAPOR RNA-binding protein BRUNOL-3 |
Gene namesi | Name:CELF2 Synonyms:BRUNOL3, CUGBP2, ETR3, NAPOR |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2550, CELF2 |
MIMi | 602538, gene |
neXtProti | NX_O95319 |
VEuPathDBi | HostDB:ENSG00000048740.17 |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 10659 |
OpenTargetsi | ENSG00000048740 |
PharmGKBi | PA27046 |
Miscellaneous databases
Pharosi | O95319, Tbio |
Genetic variation databases
BioMutai | CELF2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000295189 | 1 – 508 | CUGBP Elav-like family member 2Add BLAST | 508 |
Proteomic databases
jPOSTi | O95319 |
MassIVEi | O95319 |
MaxQBi | O95319 |
PaxDbi | O95319 |
PeptideAtlasi | O95319 |
PRIDEi | O95319 |
ProteomicsDBi | 50802 [O95319-1] 50803 [O95319-2] 50804 [O95319-3] 50805 [O95319-4] 50806 [O95319-5] |
2D gel databases
UCD-2DPAGEi | O95319 |
PTM databases
GlyGeni | O95319, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O95319 |
MetOSitei | O95319 |
PhosphoSitePlusi | O95319 |
Expressioni
Tissue specificityi
Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.5 Publications
Developmental stagei
Isoform 1 is expressed in fetal brain. Isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney and spleen. Isoform 4 is expressed in fetal heart, brain, thymus, lung and skeletal muscle.2 Publications
Gene expression databases
Bgeei | ENSG00000048740, Expressed in forebrain and 253 other tissues |
ExpressionAtlasi | O95319, baseline and differential |
Genevisiblei | O95319, HS |
Organism-specific databases
HPAi | ENSG00000048740, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with A1CF.
By similarityProtein-protein interaction databases
BioGRIDi | 115902, 27 interactors |
IntActi | O95319, 8 interactors |
MINTi | O95319 |
STRINGi | 9606.ENSP00000443926 |
Miscellaneous databases
RNActi | O95319, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | O95319 |
SMRi | O95319 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 123 | RRM 1PROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 132 – 212 | RRM 2PROSITE-ProRule annotationAdd BLAST | 81 | |
Domaini | 423 – 501 | RRM 3PROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 283 | Necessary for RNA-binding, TNNT2 exon 5 and NMDA R1 exon 21 inclusionAdd BLAST | 283 | |
Regioni | 357 – 508 | Necessary for RNA-binding, TNNT2 exon 5 and NMDA R1 exon 21 inclusionAdd BLAST | 152 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 284 – 397 | Ala-richAdd BLAST | 114 |
Sequence similaritiesi
Belongs to the CELF/BRUNOL family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0144, Eukaryota |
GeneTreei | ENSGT00940000155461 |
HOGENOMi | CLU_015367_0_2_1 |
InParanoidi | O95319 |
OrthoDBi | 1209165at2759 |
PhylomeDBi | O95319 |
Family and domain databases
CDDi | cd12631, RRM1_CELF1_2_Bruno, 1 hit cd12634, RRM2_CELF1_2, 1 hit cd12638, RRM3_CELF1_2, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
InterProi | View protein in InterPro IPR034196, CELF1/2_RRM1 IPR034198, CELF1/2_RRM2 IPR034199, CELF1/2_RRM3 IPR002343, Hud_Sxl_RNA IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
PRINTSi | PR00961, HUDSXLRNA |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O95319-1) [UniParc]FASTAAdd to basket
Also known as: NAPOR-3
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRCPKSAVTM RNEELLLSNG TANKMNGALD HSDQPDPDAI KMFVGQIPRS
60 70 80 90 100
WSEKELKELF EPYGAVYQIN VLRDRSQNPP QSKGCCFVTF YTRKAALEAQ
110 120 130 140 150
NALHNIKTLP GMHHPIQMKP ADSEKSNAVE DRKLFIGMVS KKCNENDIRV
160 170 180 190 200
MFSPFGQIEE CRILRGPDGL SRGCAFVTFS TRAMAQNAIK AMHQSQTMEG
210 220 230 240 250
CSSPIVVKFA DTQKDKEQRR LQQQLAQQMQ QLNTATWGNL TGLGGLTPQY
260 270 280 290 300
LALLQQATSS SNLGAFSGIQ QMAGMNALQL QNLATLAAAA AAAQTSATST
310 320 330 340 350
NANPLSTTSS ALGALTSPVA ASTPNSTAGA AMNSLTSLGT LQGLAGATVG
360 370 380 390 400
LNNINALAGM AALNGGLGAT GLTNGTAGTM DALTQAYSGI QQYAAAALPT
410 420 430 440 450
LYSQSLLQQQ SAAGSQKEGP EGANLFIYHL PQEFGDQDIL QMFMPFGNVI
460 470 480 490 500
SAKVFIDKQT NLSKCFGFVS YDNPVSAQAA IQAMNGFQIG MKRLKVQLKR
SKNDSKPY
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GU44 | A0A1B0GU44_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 520 | Annotation score: | ||
A0A1B0GUN8 | A0A1B0GUN8_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 526 | Annotation score: | ||
E9PC62 | E9PC62_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 521 | Annotation score: | ||
A0A0J9YX66 | A0A0J9YX66_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 514 | Annotation score: | ||
A0A0J9YXJ0 | A0A0J9YXJ0_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 533 | Annotation score: | ||
Q5VZZ6 | Q5VZZ6_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 502 | Annotation score: | ||
V9GYD9 | V9GYD9_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 440 | Annotation score: | ||
V9GY47 | V9GY47_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 70 | Annotation score: | ||
A0A0J9YXK1 | A0A0J9YXK1_HUMAN | CUGBP Elav-like family member 2 | CELF2 | 94 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052202 | 438 | D → H2 PublicationsCorresponds to variant dbSNP:rs1050942Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_026796 | 1 – 24 | Missing in isoform 2 and isoform 5. 4 PublicationsAdd BLAST | 24 | |
Alternative sequenceiVSP_026797 | 1 – 18 | MRCPK…ELLLS → MMVEGRLLVPDRI in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 18 | |
Alternative sequenceiVSP_026798 | 358 | A → AVAQMLS in isoform 2 and isoform 4. 3 Publications | 1 | |
Alternative sequenceiVSP_026799 | 358 | A → AGTINTPRSKRLLLPKDNN in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_026800 | 359 | G → GTINS in isoform 5. 2 Publications | 1 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000399850; ENSP00000382743; ENSG00000048740 [O95319-2] ENST00000416382; ENSP00000406451; ENSG00000048740 [O95319-1] ENST00000417956; ENSP00000404834; ENSG00000048740 [O95319-2] ENST00000608830; ENSP00000476999; ENSG00000048740 [O95319-5] ENST00000631460; ENSP00000488582; ENSG00000048740 [O95319-1] ENST00000632728; ENSP00000487802; ENSG00000048740 [O95319-2] ENST00000638035; ENSP00000490401; ENSG00000048740 [O95319-2] |
GeneIDi | 10659 |
KEGGi | hsa:10659 |
UCSCi | uc031vxc.2, human [O95319-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MY7 | NMR | - | A | 416-508 | [»] | |
2MY8 | NMR | - | A | 416-508 | [»] | |
4LJM | X-ray | 3.00 | A/B | 416-508 | [»] | |
4LMZ | X-ray | 2.78 | A | 36-211 | [»] | |
4TLQ | X-ray | 2.50 | A/B | 416-508 | [»] | |
5M8I | NMR | - | A | 416-508 | [»] | |
BMRBi | O95319 | |||||
SMRi | O95319 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115902, 27 interactors |
IntActi | O95319, 8 interactors |
MINTi | O95319 |
STRINGi | 9606.ENSP00000443926 |
PTM databases
GlyGeni | O95319, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O95319 |
MetOSitei | O95319 |
PhosphoSitePlusi | O95319 |
Genetic variation databases
BioMutai | CELF2 |
2D gel databases
UCD-2DPAGEi | O95319 |
Proteomic databases
jPOSTi | O95319 |
MassIVEi | O95319 |
MaxQBi | O95319 |
PaxDbi | O95319 |
PeptideAtlasi | O95319 |
PRIDEi | O95319 |
ProteomicsDBi | 50802 [O95319-1] 50803 [O95319-2] 50804 [O95319-3] 50805 [O95319-4] 50806 [O95319-5] |
Protocols and materials databases
Antibodypediai | 5602, 222 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 10659 |
DisGeNETi | 10659 |
GeneCardsi | CELF2 |
HGNCi | HGNC:2550, CELF2 |
HPAi | ENSG00000048740, Low tissue specificity |
MIMi | 602538, gene |
neXtProti | NX_O95319 |
OpenTargetsi | ENSG00000048740 |
PharmGKBi | PA27046 |
VEuPathDBi | HostDB:ENSG00000048740.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0144, Eukaryota |
GeneTreei | ENSGT00940000155461 |
HOGENOMi | CLU_015367_0_2_1 |
InParanoidi | O95319 |
OrthoDBi | 1209165at2759 |
PhylomeDBi | O95319 |
Enzyme and pathway databases
PathwayCommonsi | O95319 |
SIGNORi | O95319 |
Miscellaneous databases
BioGRID-ORCSi | 10659, 6 hits in 879 CRISPR screens |
ChiTaRSi | CELF2, human |
GeneWikii | CUGBP2 |
GenomeRNAii | 10659 |
Pharosi | O95319, Tbio |
PROi | PR:O95319 |
RNActi | O95319, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000048740, Expressed in forebrain and 253 other tissues |
ExpressionAtlasi | O95319, baseline and differential |
Genevisiblei | O95319, HS |
Family and domain databases
CDDi | cd12631, RRM1_CELF1_2_Bruno, 1 hit cd12634, RRM2_CELF1_2, 1 hit cd12638, RRM3_CELF1_2, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
InterProi | View protein in InterPro IPR034196, CELF1/2_RRM1 IPR034198, CELF1/2_RRM2 IPR034199, CELF1/2_RRM3 IPR002343, Hud_Sxl_RNA IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
PRINTSi | PR00961, HUDSXLRNA |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CELF2_HUMAN | |
Accessioni | O95319Primary (citable) accession number: O95319 Secondary accession number(s): B7ZAN9 Q9UL67 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 10, 2007 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human entries with genetic variants
List of human entries with genetic variants