UniProtKB - O95295 (SNAPN_HUMAN)
SNARE-associated protein Snapin
SNAPIN
Functioni
Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells (PubMed:17182842, PubMed:18167355).
As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (PubMed:25898167).
3 PublicationsGO - Molecular functioni
- SNARE binding Source: MGI
GO - Biological processi
- anterograde axonal transport Source: UniProtKB
- anterograde synaptic vesicle transport Source: UniProtKB
- autophagosome maturation Source: Ensembl
- endosome to lysosome transport Source: MGI
- intracellular protein transport Source: ProtInc
- late endosome to lysosome transport Source: Ensembl
- lysosomal lumen acidification Source: Ensembl
- lysosome localization Source: UniProtKB
- lysosome organization Source: GO_Central
- melanosome organization Source: UniProtKB
- negative regulation of neuron projection development Source: Ensembl
- neuron projection development Source: UniProtKB
- neurotransmitter secretion Source: ProtInc
- organelle transport along microtubule Source: ComplexPortal
- positive regulation of late endosome to lysosome transport Source: ParkinsonsUK-UCL
- protein-containing complex localization Source: Ensembl
- protein maturation Source: Ensembl
- regulation of endosome size Source: ComplexPortal
- regulation of lysosome size Source: ComplexPortal
- regulation of protein binding Source: ParkinsonsUK-UCL
- regulation of synaptic vesicle exocytosis Source: GO_Central
- retrograde axonal transport Source: Ensembl
- synaptic vesicle exocytosis Source: MGI
- synaptic vesicle fusion to presynaptic active zone membrane Source: Ensembl
- synaptic vesicle maturation Source: Ensembl
- synaptic vesicle transport Source: UniProtKB
- terminal button organization Source: Ensembl
Keywordsi
Biological process | Exocytosis, Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | O95295 |
Reactomei | R-HSA-432722, Golgi Associated Vesicle Biogenesis |
SignaLinki | O95295 |
SIGNORi | O95295 |
Protein family/group databases
TCDBi | 1.F.1.1.1, the synaptosomal vesicle fusion pore (svf-pore) family |
Names & Taxonomyi
Protein namesi | Recommended name: SNARE-associated protein SnapinAlternative name(s): Biogenesis of lysosome-related organelles complex 1 subunit 7 Short name: BLOC-1 subunit 7 Synaptosomal-associated protein 25-binding protein Short name: SNAP-associated protein |
Gene namesi | Name:SNAPIN Synonyms:BLOC1S7, SNAP25BP, SNAPAP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17145, SNAPIN |
MIMi | 607007, gene |
neXtProti | NX_O95295 |
VEuPathDBi | HostDB:ENSG00000143553 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity
Cytoplasm and Cytosol
- cytosol By similarity
Lysosome
- Lysosome membrane 1 Publication
Other locations
- Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- perinuclear region 3 Publications
- synaptic vesicle membrane 1 Publication
Note: Colocalizes with NANOS1 and PUM2 in the perinuclear region of germ cells.1 Publication
Cytoskeleton
- manchette Source: Ensembl
Cytosol
- BLOC-1 complex Source: UniProtKB
- BORC complex Source: UniProtKB
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Lysosome
- cytoplasmic side of lysosomal membrane Source: ComplexPortal
Other locations
- acrosomal vesicle Source: Ensembl
- axon cytoplasm Source: GOC
- perinuclear region of cytoplasm Source: UniProtKB
- secretory granule Source: UniProtKB
- synapse Source: MGI
- synaptic vesicle Source: UniProtKB
- synaptic vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Lysosome, Membrane, SynapsePathology & Biotechi
Organism-specific databases
DisGeNETi | 23557 |
OpenTargetsi | ENSG00000143553 |
PharmGKBi | PA162404012 |
Miscellaneous databases
Pharosi | O95295, Tbio |
Genetic variation databases
BioMutai | SNAPIN |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000097556 | 2 – 136 | SNARE-associated protein SnapinAdd BLAST | 135 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 10 | PhosphoserineCombined sources | 1 | |
Modified residuei | 14 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 50 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 126 | PhosphoserineCombined sources | 1 | |
Modified residuei | 129 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 133 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O95295 |
jPOSTi | O95295 |
MassIVEi | O95295 |
MaxQBi | O95295 |
PaxDbi | O95295 |
PeptideAtlasi | O95295 |
PRIDEi | O95295 |
ProteomicsDBi | 50792 |
PTM databases
GlyGeni | O95295, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O95295 |
PhosphoSitePlusi | O95295 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSG00000143553, Expressed in quadriceps femoris and 215 other tissues |
Genevisiblei | O95295, HS |
Organism-specific databases
HPAi | ENSG00000143553, Low tissue specificity |
Interactioni
Subunit structurei
Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos (PubMed:15102850, PubMed:22203680).
Component of the BLOC-one-related complex (BORC) which is composed of BLOC1S1, BLOC1S2, BORCS5, BORCS6, BORCS7, BORCS8, KXD1 and SNAPIN (PubMed:25898167). Associates with the SNARE complex.
Interacts with CSNK1D, SNAP23 and STX4A but not with STX1A, VAMP2 and SYT1.
Interacts with SNAP25; the interaction with SNAP25 is increased by its phosphorylation.
Interacts with CNTRL, NANOS1, PUM2 and RGS7 (PubMed:12659861, PubMed:16213214, PubMed:19168546).
Interacts with TOR1A; the interaction is direct and associates SNAPIN with the CSN complex (PubMed:18167355, PubMed:21102408).
8 Publications(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL70.
1 PublicationBinary interactionsi
O95295
GO - Molecular functioni
- SNARE binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 117101, 150 interactors |
ComplexPortali | CPX-1910, BLOC-1 complex CPX-5029, BORC complex |
CORUMi | O95295 |
IntActi | O95295, 121 interactors |
MINTi | O95295 |
STRINGi | 9606.ENSP00000357674 |
Miscellaneous databases
RNActi | O95295, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 83 – 136 | Interaction with TOR1AAdd BLAST | 54 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 37 – 126 | Sequence analysisAdd BLAST | 90 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502S07W, Eukaryota |
GeneTreei | ENSGT00390000008274 |
HOGENOMi | CLU_124640_1_0_1 |
InParanoidi | O95295 |
OMAi | QLDTHVH |
OrthoDBi | 1532513at2759 |
PhylomeDBi | O95295 |
TreeFami | TF319577 |
Family and domain databases
InterProi | View protein in InterPro IPR017246, Snapin IPR028119, Snapin/Pallidin/Snn1 |
PANTHERi | PTHR31305, PTHR31305, 1 hit |
Pfami | View protein in Pfam PF14712, Snapin_Pallidin, 1 hit |
PIRSFi | PIRSF037631, Snapin, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAGAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES
60 70 80 90 100
QVELREQIDN LATELCRINE DQKVALDLDP YVKKLLNARR RVVLVNNILQ
110 120 130
NAQERLRRLN HSVAKETARR RAMLDSGIYP PGSPGK
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_017423 | 112 | S → C. Corresponds to variant dbSNP:rs1802461Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF086837 mRNA Translation: AAD11417.1 AK024555 mRNA Translation: BAB14927.1 BT006753 mRNA Translation: AAP35399.1 AL592150 Genomic DNA Translation: CAI18797.1 CH471121 Genomic DNA Translation: EAW53288.1 CH471121 Genomic DNA Translation: EAW53289.1 BC000761 mRNA Translation: AAH00761.1 BC004494 mRNA Translation: AAH04494.1 |
CCDSi | CCDS1049.1 |
RefSeqi | NP_036569.1, NM_012437.5 |
Genome annotation databases
Ensembli | ENST00000368685.6; ENSP00000357674.5; ENSG00000143553.11 |
GeneIDi | 23557 |
KEGGi | hsa:23557 |
MANE-Selecti | ENST00000368685.6; ENSP00000357674.5; NM_012437.6; NP_036569.1 |
UCSCi | uc001fcq.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF086837 mRNA Translation: AAD11417.1 AK024555 mRNA Translation: BAB14927.1 BT006753 mRNA Translation: AAP35399.1 AL592150 Genomic DNA Translation: CAI18797.1 CH471121 Genomic DNA Translation: EAW53288.1 CH471121 Genomic DNA Translation: EAW53289.1 BC000761 mRNA Translation: AAH00761.1 BC004494 mRNA Translation: AAH04494.1 |
CCDSi | CCDS1049.1 |
RefSeqi | NP_036569.1, NM_012437.5 |
3D structure databases
AlphaFoldDBi | O95295 |
SMRi | O95295 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 117101, 150 interactors |
ComplexPortali | CPX-1910, BLOC-1 complex CPX-5029, BORC complex |
CORUMi | O95295 |
IntActi | O95295, 121 interactors |
MINTi | O95295 |
STRINGi | 9606.ENSP00000357674 |
Protein family/group databases
TCDBi | 1.F.1.1.1, the synaptosomal vesicle fusion pore (svf-pore) family |
PTM databases
GlyGeni | O95295, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O95295 |
PhosphoSitePlusi | O95295 |
Genetic variation databases
BioMutai | SNAPIN |
Proteomic databases
EPDi | O95295 |
jPOSTi | O95295 |
MassIVEi | O95295 |
MaxQBi | O95295 |
PaxDbi | O95295 |
PeptideAtlasi | O95295 |
PRIDEi | O95295 |
ProteomicsDBi | 50792 |
Protocols and materials databases
ABCDi | O95295, 1 sequenced antibody |
Antibodypediai | 34134, 135 antibodies from 29 providers |
DNASUi | 23557 |
Genome annotation databases
Ensembli | ENST00000368685.6; ENSP00000357674.5; ENSG00000143553.11 |
GeneIDi | 23557 |
KEGGi | hsa:23557 |
MANE-Selecti | ENST00000368685.6; ENSP00000357674.5; NM_012437.6; NP_036569.1 |
UCSCi | uc001fcq.5, human |
Organism-specific databases
CTDi | 23557 |
DisGeNETi | 23557 |
GeneCardsi | SNAPIN |
HGNCi | HGNC:17145, SNAPIN |
HPAi | ENSG00000143553, Low tissue specificity |
MIMi | 607007, gene |
neXtProti | NX_O95295 |
OpenTargetsi | ENSG00000143553 |
PharmGKBi | PA162404012 |
VEuPathDBi | HostDB:ENSG00000143553 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S07W, Eukaryota |
GeneTreei | ENSGT00390000008274 |
HOGENOMi | CLU_124640_1_0_1 |
InParanoidi | O95295 |
OMAi | QLDTHVH |
OrthoDBi | 1532513at2759 |
PhylomeDBi | O95295 |
TreeFami | TF319577 |
Enzyme and pathway databases
PathwayCommonsi | O95295 |
Reactomei | R-HSA-432722, Golgi Associated Vesicle Biogenesis |
SignaLinki | O95295 |
SIGNORi | O95295 |
Miscellaneous databases
BioGRID-ORCSi | 23557, 107 hits in 1078 CRISPR screens |
ChiTaRSi | SNAPIN, human |
GeneWikii | SNAPAP |
GenomeRNAii | 23557 |
Pharosi | O95295, Tbio |
PROi | PR:O95295 |
RNActi | O95295, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000143553, Expressed in quadriceps femoris and 215 other tissues |
Genevisiblei | O95295, HS |
Family and domain databases
InterProi | View protein in InterPro IPR017246, Snapin IPR028119, Snapin/Pallidin/Snn1 |
PANTHERi | PTHR31305, PTHR31305, 1 hit |
Pfami | View protein in Pfam PF14712, Snapin_Pallidin, 1 hit |
PIRSFi | PIRSF037631, Snapin, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SNAPN_HUMAN | |
Accessioni | O95295Primary (citable) accession number: O95295 Secondary accession number(s): D3DV56, Q5SXU8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families