Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - O95239 (KIF4A_HUMAN)

Protein

Chromosome-associated kinesin KIF4A

Gene

KIF4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 95ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • microtubule binding Source: GO_Central
  • microtubule motor activity Source: GO_Central
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionDNA-binding, Motor protein
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2132295 MHC class II antigen presentation
R-HSA-437239 Recycling pathway of L1
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome-associated kinesin KIF4A
Alternative name(s):
Chromokinesin-A
Gene namesi
Name:KIF4A
Synonyms:KIF4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000090889.11
HGNCiHGNC:13339 KIF4A
MIMi300521 gene
neXtProtiNX_O95239

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Involvement in diseasei

Mental retardation, X-linked 100 (MRX100)1 Publication
The disease may be caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation, while syndromic mental retardation presents with associated physical, neurological and/or psychiatric manifestations. MRX100 clinical features include intellectual disability, epilepsy, microcephaly and cortical malformations.
See also OMIM:300923

Keywords - Diseasei

Mental retardation

Organism-specific databases

DisGeNETi24137
MalaCardsiKIF4A
MIMi300923 phenotype
OpenTargetsiENSG00000090889
PharmGKBiPA30105

Chemistry databases

ChEMBLiCHEMBL6163

Polymorphism and mutation databases

BioMutaiKIF4A

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001254371 – 1232Chromosome-associated kinesin KIF4AAdd BLAST1232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei394PhosphoserineBy similarity1
Modified residuei799PhosphothreonineCombined sources1
Modified residuei801PhosphoserineCombined sources1
Modified residuei810PhosphoserineBy similarity1
Modified residuei815PhosphoserineCombined sources1
Modified residuei951PhosphoserineCombined sources1
Modified residuei995PhosphothreonineCombined sources1
Modified residuei1001PhosphoserineCombined sources1
Modified residuei1013PhosphoserineCombined sources1
Modified residuei1017PhosphoserineCombined sources1
Modified residuei1028PhosphoserineCombined sources1
Modified residuei1126PhosphoserineCombined sources1
Modified residuei1181PhosphothreonineCombined sources1
Modified residuei1186PhosphoserineCombined sources1
Cross-linki1194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1225PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO95239
MaxQBiO95239
PaxDbiO95239
PeptideAtlasiO95239
PRIDEiO95239
ProteomicsDBi50738
50739 [O95239-2]

PTM databases

iPTMnetiO95239
PhosphoSitePlusiO95239

Expressioni

Tissue specificityi

Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.1 Publication

Gene expression databases

BgeeiENSG00000090889 Expressed in 121 organ(s), highest expression level in oocyte
CleanExiHS_KIF4A
GenevisibleiO95239 HS

Organism-specific databases

HPAiHPA034745
HPA034746
HPA061583

Interactioni

Subunit structurei

Interacts with unphosphorylated PRC1 during late mitosis.2 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi117288, 26 interactors
CORUMiO95239
ELMiO95239
IntActiO95239, 13 interactors
MINTiO95239
STRINGi9606.ENSP00000363524

Structurei

3D structure databases

ProteinModelPortaliO95239
SMRiO95239
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 336Kinesin motorPROSITE-ProRule annotationAdd BLAST328

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni663 – 1232Interaction with PRC1Add BLAST570
Regioni1000 – 1232GlobularAdd BLAST233

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili350 – 999Sequence analysisAdd BLAST650

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi793 – 798Nuclear localization signal6

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Chromokinesin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0244 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150846
HOVERGENiHBG052256
InParanoidiO95239
KOiK10395
OMAiACKEGSH
OrthoDBiEOG091G02I4
PhylomeDBiO95239
TreeFamiTF105224

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O95239-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKEEVKGIPV RVALRCRPLV PKEISEGCQM CLSFVPGEPQ VVVGTDKSFT 
        60         70         80         90        100
YDFVFDPSTE QEEVFNTAVA PLIKGVFKGY NATVLAYGQT GSGKTYSMGG 
       110        120        130        140        150
AYTAEQENEP TVGVIPRVIQ LLFKEIDKKS DFEFTLKVSY LEIYNEEILD 
       160        170        180        190        200
LLCPSREKAQ INIREDPKEG IKIVGLTEKT VLVALDTVSC LEQGNNSRTV 
       210        220        230        240        250
ASTAMNSQSS RSHAIFTISL EQRKKSDKNS SFRSKLHLVD LAGSERQKKT 
       260        270        280        290        300
KAEGDRLKEG ININRGLLCL GNVISALGDD KKGGFVPYRD SKLTRLLQDS 
       310        320        330        340        350
LGGNSHTLMI ACVSPADSNL EETLNTLRYA DRARKIKNKP IVNIDPQTAE 
       360        370        380        390        400
LNHLKQQVQQ LQVLLLQAHG GTLPGSITVE PSENLQSLME KNQSLVEENE 
       410        420        430        440        450
KLSRGLSEAA GQTAQMLERI ILTEQANEKM NAKLEELRQH AACKLDLQKL 
       460        470        480        490        500
VETLEDQELK ENVEIICNLQ QLITQLSDET VACMAAAIDT AVEQEAQVET 
       510        520        530        540        550
SPETSRSSDA FTTQHALRQA QMSKELVELN KALALKEALA RKMTQNDSQL 
       560        570        580        590        600
QPIQYQYQDN IKELELEVIN LQKEKEELVL ELQTAKKDAN QAKLSERRRK 
       610        620        630        640        650
RLQELEGQIA DLKKKLNEQS KLLKLKESTE RTVSKLNQEI RMMKNQRVQL 
       660        670        680        690        700
MRQMKEDAEK FRQWKQKKDK EVIQLKERDR KRQYELLKLE RNFQKQSNVL 
       710        720        730        740        750
RRKTEEAAAA NKRLKDALQK QREVADKRKE TQSRGMEGTA ARVKNWLGNE 
       760        770        780        790        800
IEVMVSTEEA KRHLNDLLED RKILAQDVAQ LKEKKESGEN PPPKLRRRTF 
       810        820        830        840        850
SLTEVRGQVS ESEDSITKQI ESLETEMEFR SAQIADLQQK LLDAESEDRP 
       860        870        880        890        900
KQRWENIATI LEAKCALKYL IGELVSSKIQ VSKLESSLKQ SKTSCADMQK 
       910        920        930        940        950
MLFEERNHFA EIETELQAEL VRMEQQHQEK VLYLLSQLQQ SQMAEKQLEE 
       960        970        980        990       1000
SVSEKEQQLL STLKCQDEEL EKMREVCEQN QQLLRENEII KQKLTLLQVA 
      1010       1020       1030       1040       1050
SRQKHLPKDT LLSPDSSFEY VPPKPKPSRV KEKFLEQSMD IEDLKYCSEH 
      1060       1070       1080       1090       1100
SVNEHEDGDG DDDEGDDEEW KPTKLVKVSR KNIQGCSCKG WCGNKQCGCR 
      1110       1120       1130       1140       1150
KQKSDCGVDC CCDPTKCRNR QQGKDSLGTV ERTQDSEGSF KLEDPTEVTP 
      1160       1170       1180       1190       1200
GLSFFNPVCA TPNSKILKEM CDVEQVLSKK TPPAPSPFDL PELKHVATEY 
      1210       1220       1230 
QENKAPGKKK KRALASNTSF FSGCSPIEEE AH
Length:1,232
Mass (Da):139,881
Last modified:April 12, 2005 - v3
Checksum:iAD1B7C2A10AB24DB
GO
Isoform 2 (identifier: O95239-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1086-1127: CSCKGWCGNK...RNRQQGKDSL → VLLLTPVISA...RDPVSTKPKN
     1128-1232: Missing.

Note: No experimental confirmation available.
Show »
Length:1,127
Mass (Da):128,459
Checksum:i84D7ABE2CE39697A
GO

Sequence cautioni

The sequence AAH49218 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223R → G in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti231S → T in AAF86334 (PubMed:9168136).Curated1
Sequence conflicti286V → A in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti564L → H in AAF86334 (PubMed:9168136).Curated1
Sequence conflicti564L → P in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti600K → E in CAB75427 (Ref. 3) Curated1
Sequence conflicti668K → R (Ref. 1) Curated1
Sequence conflicti668K → R (PubMed:10978527).Curated1
Sequence conflicti928Q → P in AAD51855 (Ref. 1) Curated1
Sequence conflicti958Q → R in CAB75427 (Ref. 3) Curated1
Sequence conflicti960L → Q in AAD51855 (Ref. 1) Curated1
Sequence conflicti996 – 997LL → S in AAF86334 (PubMed:9168136).Curated2
Sequence conflicti1003 – 1014QKHLP…TLLSP → RTLPRIPFYLQ in AAF86334 (PubMed:9168136).CuratedAdd BLAST12
Sequence conflicti1022P → Q in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti1077K → N in AAD05492 (PubMed:10978527).Curated1
Sequence conflicti1138G → S in AAD05492 (PubMed:10978527).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021828422L → W2 PublicationsCorresponds to variant dbSNP:rs1199457Ensembl.1
Natural variantiVAR_049693491A → V. Corresponds to variant dbSNP:rs2297871Ensembl.1
Natural variantiVAR_0496941193L → S. Corresponds to variant dbSNP:rs1046485Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0133751086 – 1127CSCKG…GKDSL → VLLLTPVISALWEAEARGLL EARSSRPAWATWRDPVSTKP KN in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_0133761128 – 1232Missing in isoform 2. 1 PublicationAdd BLAST105

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF179308 mRNA Translation: AAD51855.1
AF071592 mRNA Translation: AAD05492.2
AJ271784 mRNA Translation: CAB75427.1
AK313133 mRNA Translation: BAG35952.1
AL139398 Genomic DNA No translation available.
AL357752 Genomic DNA No translation available.
CH471132 Genomic DNA Translation: EAX05341.1
CH471132 Genomic DNA Translation: EAX05342.1
BC049218 mRNA Translation: AAH49218.1 Sequence problems.
BC050548 mRNA Translation: AAH50548.1
AF277375 mRNA Translation: AAF86334.1
CCDSiCCDS14401.1 [O95239-1]
RefSeqiNP_036442.3, NM_012310.4 [O95239-1]
UniGeneiHs.648326

Genome annotation databases

EnsembliENST00000374403; ENSP00000363524; ENSG00000090889 [O95239-1]
GeneIDi24137
KEGGihsa:24137
UCSCiuc004dyg.4 human [O95239-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF179308 mRNA Translation: AAD51855.1
AF071592 mRNA Translation: AAD05492.2
AJ271784 mRNA Translation: CAB75427.1
AK313133 mRNA Translation: BAG35952.1
AL139398 Genomic DNA No translation available.
AL357752 Genomic DNA No translation available.
CH471132 Genomic DNA Translation: EAX05341.1
CH471132 Genomic DNA Translation: EAX05342.1
BC049218 mRNA Translation: AAH49218.1 Sequence problems.
BC050548 mRNA Translation: AAH50548.1
AF277375 mRNA Translation: AAF86334.1
CCDSiCCDS14401.1 [O95239-1]
RefSeqiNP_036442.3, NM_012310.4 [O95239-1]
UniGeneiHs.648326

3D structure databases

ProteinModelPortaliO95239
SMRiO95239
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117288, 26 interactors
CORUMiO95239
ELMiO95239
IntActiO95239, 13 interactors
MINTiO95239
STRINGi9606.ENSP00000363524

Chemistry databases

ChEMBLiCHEMBL6163

PTM databases

iPTMnetiO95239
PhosphoSitePlusiO95239

Polymorphism and mutation databases

BioMutaiKIF4A

Proteomic databases

EPDiO95239
MaxQBiO95239
PaxDbiO95239
PeptideAtlasiO95239
PRIDEiO95239
ProteomicsDBi50738
50739 [O95239-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374403; ENSP00000363524; ENSG00000090889 [O95239-1]
GeneIDi24137
KEGGihsa:24137
UCSCiuc004dyg.4 human [O95239-1]

Organism-specific databases

CTDi24137
DisGeNETi24137
EuPathDBiHostDB:ENSG00000090889.11
GeneCardsiKIF4A
H-InvDBiHIX0016851
HGNCiHGNC:13339 KIF4A
HPAiHPA034745
HPA034746
HPA061583
MalaCardsiKIF4A
MIMi300521 gene
300923 phenotype
neXtProtiNX_O95239
OpenTargetsiENSG00000090889
PharmGKBiPA30105
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0244 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150846
HOVERGENiHBG052256
InParanoidiO95239
KOiK10395
OMAiACKEGSH
OrthoDBiEOG091G02I4
PhylomeDBiO95239
TreeFamiTF105224

Enzyme and pathway databases

ReactomeiR-HSA-2132295 MHC class II antigen presentation
R-HSA-437239 Recycling pathway of L1
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-983189 Kinesins

Miscellaneous databases

ChiTaRSiKIF4A human
GeneWikiiKIF4A
GenomeRNAii24137
PROiPR:O95239
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000090889 Expressed in 121 organ(s), highest expression level in oocyte
CleanExiHS_KIF4A
GenevisibleiO95239 HS

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKIF4A_HUMAN
AccessioniPrimary (citable) accession number: O95239
Secondary accession number(s): B2R7V5
, D3DVU4, Q86TN3, Q86XX7, Q9NNY6, Q9NY24, Q9UMW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: April 12, 2005
Last modified: November 7, 2018
This is version 180 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again