ZWINT

Functionⁱ

Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.4 Publications

GO - Molecular functionⁱ

protein N-terminus binding
Source: HGNC
Inferred from physical interactionⁱ
- 15485811

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell division Source: UniProtKB-KW
establishment of localization in cell
Source: HGNC
Inferred from direct assayⁱ
- 15485811
mitotic cell cycle checkpoint
Source: HGNC
Inferred from direct assayⁱ
- 15485811
mitotic sister chromatid segregation
Source: HGNC
Inferred from direct assayⁱ
- 15485811

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell cycle, Cell division, Mitosis

Biological process

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

Reactomeⁱ	R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813 Separation of Sister Chromatids R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-5663220 RHO GTPases Activate Formins R-HSA-68877 Mitotic Prometaphase

Reactomeⁱ

R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ZW10 interactor Alternative name(s): ZW10-interacting protein 1 Short name: Zwint-1
Gene namesⁱ	Name:ZWINT
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 10

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000122952.16
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:13195 ZWINT
MIMⁱ	609177 gene
neXtProtⁱ	NX_O95229

Keywords - Cellular componentⁱ

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	11130
Open Targets More...OpenTargetsⁱ	ENSG00000122952
PharmGKBⁱ	PA37761

Polymorphism and mutation databases

BioMutaⁱ	ZWINT

BioMutaⁱ

ZWINT

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000066594	1 – 277	ZW10 interactorAdd BLAST		277

Proteomic databases

EPDⁱ	O95229
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O95229
PaxDbⁱ	O95229
PeptideAtlas More...PeptideAtlasⁱ	O95229
PRIDEⁱ	O95229
ProteomicsDBⁱ	50728

PTM databases

iPTMnetⁱ	O95229
PhosphoSitePlusⁱ	O95229

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000122952 Expressed in 195 organ(s), highest expression level in oocyte
CleanExⁱ	HS_ZWINT
ExpressionAtlasⁱ	O95229 baseline and differential
Genevisibleⁱ	O95229 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB033926 HPA005757 HPA022264

HPAⁱ

CAB033926
HPA005757
HPA022264

Interactionⁱ

Subunit structureⁱ

Interacts with ZW10 and MIS12. Interacts with the NDC80 subunit of the NDC80 complex specifically during mitosis. Also interacts with KNL1, CETN3, DSN1 and PMF1.4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
BECN1	Q14457	6	EBI-1001132,EBI-949378
NDC80	O14777	13	EBI-1001132,EBI-715849
ZW10	O43264	6	EBI-1001132,EBI-1001217

GO - Molecular functionⁱ

protein N-terminus binding
Source: HGNC
Inferred from physical interactionⁱ
- 15485811

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	116303, 117 interactors
CORUMⁱ	O95229
IntActⁱ	O95229, 90 interactors
Molecular INTeraction database More...MINTⁱ	O95229
STRINGⁱ	9606.ENSP00000363055

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O95229
SMRⁱ	O95229
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	80 – 155	Interaction with NDC80 and ZW10Add BLAST		76

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	104 – 217	Sequence analysisAdd BLAST		114

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	ENOG410IJ58 Eukaryota ENOG4111831 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017639
HOGENOMⁱ	HOG000293254
HOVERGENⁱ	HBG079391
InParanoidⁱ	O95229
KEGG Orthology (KO) More...KOⁱ	K19943
OMAⁱ	ERKTGTQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0GKM
PhylomeDBⁱ	O95229
TreeFamⁱ	TF338101

Family and domain databases

InterProⁱ	View protein in InterPro IPR029092 Zwint-1
PANTHERⁱ	PTHR31504 PTHR31504, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF15556 Zwint, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: O95229-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD 
        60         70         80         90        100
KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK 
       110        120        130        140        150
ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR 
       160        170        180        190        200
AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG TQQELDRVFQ KLGNLKQQAE 
       210        220        230        240        250
QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP QQPTRPQEQS 
       260        270 
TGDTMGRDPG VSFKAVGLQP AGDVNLP

Length:277

Mass (Da):31,293

Last modified:October 31, 2006 - v2

Checksum:ⁱ3335FBA5731AD0DC

GO

Isoform 2 (identifier: O95229-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
174-220: Missing.

« Hide

        10         20         30         40         50
MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD 
        60         70         80         90        100
KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK 
       110        120        130        140        150
ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR 
       160        170        180        190        200
AVQNQWQLQQ EKHLQHLAEV SAEGKLLFPE AEAEAENLPD DKPQQPTRPQ 
       210        220        230
EQSTGDTMGR DPGVSFKAVG LQPAGDVNLP

Note: No experimental confirmation available.

Show »

Length:230

Mass (Da):25,544

Checksum:ⁱ673BAD09107D7E3B

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A6NH27	A6NH27_HUMAN	cDNA FLJ36489 fis, clone THYMU20181... cDNA FLJ36489 fis, clone THYMU2018126, highly similar to ZW10 interactor	ZWINT	157	Annotation score:
R4GMS7	R4GMS7_HUMAN	ZW10 interactor ZW10 interactor	ZWINT	48	Annotation score:

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_028783	4	A → S. Corresponds to variant dbSNP:rs11005328Ensembl.		1
Natural variantⁱVAR_051505	187	R → G2 PublicationsCorresponds to variant dbSNP:rs2241666Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_047660	174 – 220	Missing in isoform 2. CuratedAdd BLAST		47

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF067656 mRNA Translation: AAC78629.1 AC010996 Genomic DNA No translation available. BC000411 mRNA Translation: AAH00411.1 BC020979 mRNA Translation: AAH20979.1 BC110399 mRNA Translation: AAI10400.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS31205.1 [O95229-2] CCDS7249.1 [O95229-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001005413.1, NM_001005413.1 [O95229-2] NP_008988.2, NM_007057.3 [O95229-1] NP_127490.1, NM_032997.2 [O95229-1]
UniGeneⁱ	Hs.591363

Genome annotation databases

Ensemblⁱ	ENST00000361148; ENSP00000354921; ENSG00000122952 [O95229-2] ENST00000373944; ENSP00000363055; ENSG00000122952 [O95229-1] ENST00000395405; ENSP00000378801; ENSG00000122952 [O95229-1]
GeneIDⁱ	11130
KEGGⁱ	hsa:11130
UCSC genome browser More...UCSCⁱ	uc001jjx.2 human [O95229-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O95229	cDNA FLJ36489 fis, clone THYMU2018126, highly similar to ZW10 interactor		HUMAN		157	UniRef100_O95229

Protein	Similar proteins		Species	Score	Length	Source
O95229	cDNA FLJ36489 fis, clone THYMU2018126, highly similar to ZW10 interactor		HUMAN		157	UniRef90_O95229
Uncharacterized protein		NOMLE		281
Uncharacterized protein		PAPAN		280
ZW10 interactor (Fragment)		MACMU		127
ZW10 interactor (Fragment)		MACFA		127
+29
O95229-2	ZW10 interacting kinetochore protein		PANTR		271	UniRef90_O95229-2
ZWINT isoform 9		PONAB		231
cDNA FLJ55119, highly similar to Homo sapiens ZW10 interactor (ZWINT), transcript variant 3, mRNA		HUMAN		230
Uncharacterized protein		COLAP		230
Testis cDNA, clone: QtsA-18706, similar to human ZW10 interactor (ZWINT), transcript variant 2		MACFA		110
+20

Protein	Similar proteins		Species	Score	Length	Source
O95229	cDNA FLJ36489 fis, clone THYMU2018126, highly similar to ZW10 interactor		HUMAN		157	UniRef50_O95229
Uncharacterized protein		NOMLE		281
Uncharacterized protein		MACNE		280
Uncharacterized protein		PAPAN		280
ZW10 interactor (Fragment)		MACMU		127
+56
O95229-2	ZW10 interacting kinetochore protein		PANTR		271	UniRef50_O95229-2
ZWINT isoform 9		PONAB		231
Uncharacterized protein		COLAP		230
cDNA FLJ55119, highly similar to Homo sapiens ZW10 interactor (ZWINT), transcript variant 3, mRNA		HUMAN		230
Testis cDNA, clone: QtsA-18706, similar to human ZW10 interactor (ZWINT), transcript variant 2		MACFA		110
+28

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF067656 mRNA Translation: AAC78629.1 AC010996 Genomic DNA No translation available. BC000411 mRNA Translation: AAH00411.1 BC020979 mRNA Translation: AAH20979.1 BC110399 mRNA Translation: AAI10400.1
CCDSⁱ	CCDS31205.1 [O95229-2] CCDS7249.1 [O95229-1]
RefSeqⁱ	NP_001005413.1, NM_001005413.1 [O95229-2] NP_008988.2, NM_007057.3 [O95229-1] NP_127490.1, NM_032997.2 [O95229-1]
UniGeneⁱ	Hs.591363

3D structure databases

ProteinModelPortalⁱ	O95229
SMRⁱ	O95229
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	116303, 117 interactors
CORUMⁱ	O95229
IntActⁱ	O95229, 90 interactors
MINTⁱ	O95229
STRINGⁱ	9606.ENSP00000363055

PTM databases

iPTMnetⁱ	O95229
PhosphoSitePlusⁱ	O95229

Polymorphism and mutation databases

BioMutaⁱ	ZWINT

BioMutaⁱ

ZWINT

Proteomic databases

EPDⁱ	O95229
MaxQBⁱ	O95229
PaxDbⁱ	O95229
PeptideAtlasⁱ	O95229
PRIDEⁱ	O95229
ProteomicsDBⁱ	50728

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	11130
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000361148; ENSP00000354921; ENSG00000122952 [O95229-2] ENST00000373944; ENSP00000363055; ENSG00000122952 [O95229-1] ENST00000395405; ENSP00000378801; ENSG00000122952 [O95229-1]
GeneIDⁱ	11130
KEGGⁱ	hsa:11130
UCSCⁱ	uc001jjx.2 human [O95229-1]

Organism-specific databases

CTDⁱ	11130
DisGeNETⁱ	11130
EuPathDBⁱ	HostDB:ENSG00000122952.16
GeneCardsⁱ	ZWINT
HGNCⁱ	HGNC:13195 ZWINT
HPAⁱ	CAB033926 HPA005757 HPA022264
MIMⁱ	609177 gene
neXtProtⁱ	NX_O95229
OpenTargetsⁱ	ENSG00000122952
PharmGKBⁱ	PA37761
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IJ58 Eukaryota ENOG4111831 LUCA
GeneTreeⁱ	ENSGT00390000017639
HOGENOMⁱ	HOG000293254
HOVERGENⁱ	HBG079391
InParanoidⁱ	O95229
KOⁱ	K19943
OMAⁱ	ERKTGTQ
OrthoDBⁱ	EOG091G0GKM
PhylomeDBⁱ	O95229
TreeFamⁱ	TF338101

Enzyme and pathway databases

Reactomeⁱ	R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813 Separation of Sister Chromatids R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-5663220 RHO GTPases Activate Formins R-HSA-68877 Mitotic Prometaphase

Reactomeⁱ

R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Miscellaneous databases

GeneWikiⁱ	ZWINT
GenomeRNAiⁱ	11130
Protein Ontology More...PROⁱ	PR:O95229
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000122952 Expressed in 195 organ(s), highest expression level in oocyte
CleanExⁱ	HS_ZWINT
ExpressionAtlasⁱ	O95229 baseline and differential
Genevisibleⁱ	O95229 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR029092 Zwint-1
PANTHERⁱ	PTHR31504 PTHR31504, 1 hit
Pfamⁱ	View protein in Pfam PF15556 Zwint, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ZWINT_HUMAN
Accessionⁱ	O95229Primary (citable) accession number: O95229 Secondary accession number(s): A6NNV6, Q0D2I3, Q9BWD0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
	Last sequence update:	October 31, 2006
	Last modified:	November 7, 2018
	This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM

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UniProtKB - O95229 (ZWINT_HUMAN)

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ZW10 interactor

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Coiled coil

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ