NDUFB4

Functionⁱ

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.1 Publication

GO - Molecular functionⁱ

NADH dehydrogenase (ubiquinone) activity
Source: UniProtKB
Non-traceable author statementⁱ
- 9878551

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

mitochondrial electron transport, NADH to ubiquinone
Source: UniProtKB
Non-traceable author statementⁱ
- 9878551
mitochondrial respiratory chain complex I assembly
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 27626371
response to oxidative stress
Source: UniProtKB
Inferred from direct assayⁱ
- 12857734

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Electron transport, Respiratory chain, Transport

Biological process

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

Reactomeⁱ	R-HSA-611105 Respiratory electron transport R-HSA-6799198 Complex I biogenesis

Reactomeⁱ

R-HSA-611105 Respiratory electron transport
R-HSA-6799198 Complex I biogenesis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Alternative name(s): Complex I-B15 Short name: CI-B15 NADH-ubiquinone oxidoreductase B15 subunit
Gene namesⁱ	Name:NDUFB4
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 3

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000065518.7
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:7699 NDUFB4
MIMⁱ	603840 gene
neXtProtⁱ	NX_O95168

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	88 – 105	HelicalSequence analysisAdd BLAST		18

Keywords - Cellular componentⁱ

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechⁱ

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000065518
PharmGKBⁱ	PA31510

Chemistry databases

ChEMBLⁱ	CHEMBL2363065
Drug and drug target database More...DrugBankⁱ	DB00157 NADH

Polymorphism and mutation databases

BioMutaⁱ	NDUFB4

BioMutaⁱ

NDUFB4

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000118800	2 – 129	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4Add BLAST		128

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylserineCombined sources		1
Modified residueⁱ	26	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	O95168
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O95168
PaxDbⁱ	O95168
PeptideAtlas More...PeptideAtlasⁱ	O95168
PRIDEⁱ	O95168
ProteomicsDBⁱ	50681 50682 [O95168-2]
TopDownProteomicsⁱ	O95168-1 [O95168-1]

PTM databases

iPTMnetⁱ	O95168
PhosphoSitePlusⁱ	O95168
SwissPalmⁱ	O95168

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000065518 Expressed in 237 organ(s), highest expression level in heart right ventricle
CleanExⁱ	HS_NDUFB4
ExpressionAtlasⁱ	O95168 baseline and differential
Genevisibleⁱ	O95168 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA051739

HPAⁱ

HPA051739

Interactionⁱ

Subunit structureⁱ

Complex I is composed of 45 different subunits.2 Publications

Protein-protein interaction databases

BioGridⁱ	110790, 46 interactors
ComplexPortalⁱ	CPX-577 Mitochondrial respiratory chain complex I
CORUMⁱ	O95168
IntActⁱ	O95168, 30 interactors
Molecular INTeraction database More...MINTⁱ	O95168
STRINGⁱ	9606.ENSP00000184266

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O95168
SMRⁱ	O95168
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the complex I NDUFB4 subunit family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IZUV Eukaryota ENOG4112CXK LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007133
HOGENOMⁱ	HOG000060241
HOVERGENⁱ	HBG001159
InParanoidⁱ	O95168
KEGG Orthology (KO) More...KOⁱ	K03960
OMAⁱ	TRWTYAR
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G1578
PhylomeDBⁱ	O95168
TreeFamⁱ	TF328761

Family and domain databases

InterProⁱ	View protein in InterPro IPR009866 NADH_UbQ_OxRdtase_NDUFB4_su
PANTHERⁱ	PTHR15469 PTHR15469, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07225 NDUF_B4, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: O95168-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ 
        60         70         80         90        100
YNDPNRRGLI ENPALLRWAY ARTINVYPNF RPTPKNSLMG ALCGFGPLIF 
       110        120 
IYYIIKTERD RKEKLIQEGK LDRTFHLSY

Length:129

Mass (Da):15,209

Last modified:January 23, 2007 - v3

Checksum:ⁱE5060D0BF46FFF28

GO

Isoform 2 (identifier: O95168-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
110-129: DRKEKLIQEGKLDRTFHLSY → VSIQTRCLVFE

« Hide

        10         20         30         40         50
MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ 
        60         70         80         90        100
YNDPNRRGLI ENPALLRWAY ARTINVYPNF RPTPKNSLMG ALCGFGPLIF 
       110        120
IYYIIKTERV SIQTRCLVFE

Note: No experimental confirmation available.

Show »

Length:120

Mass (Da):14,026

Checksum:ⁱ08BB084685E3098E

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

C9JXQ9	C9JXQ9_HUMAN	NADH dehydrogenase [ubiquinone] 1 b... NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4	NDUFB4	80	Annotation score:
F2Z3P9	F2Z3P9_HUMAN	NADH dehydrogenase [ubiquinone] 1 b... NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4	NDUFB4	69	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_042719	110 – 129	DRKEK…FHLSY → VSIQTRCLVFE in isoform 2. 1 PublicationAdd BLAST		20

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF044957 mRNA Translation: AAD05421.1 AC126182 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79525.1 BC000855 mRNA Translation: AAH00855.1 BC070285 mRNA Translation: AAH70285.1 BC146885 mRNA Translation: AAI46886.1 BC146887 mRNA Translation: AAI46888.1 BC146927 mRNA Translation: AAI46928.1 BC146932 mRNA Translation: AAI46933.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS2999.1 [O95168-1] CCDS54630.1 [O95168-2]
PIRⁱ	JE0383
NCBI Reference Sequences More...RefSeqⁱ	NP_001161803.1, NM_001168331.1 [O95168-2] NP_004538.2, NM_004547.5 [O95168-1]
UniGeneⁱ	Hs.304613

Genome annotation databases

Ensemblⁱ	ENST00000184266; ENSP00000184266; ENSG00000065518 [O95168-1] ENST00000485064; ENSP00000419578; ENSG00000065518 [O95168-2]
GeneIDⁱ	4710
KEGGⁱ	hsa:4710
UCSC genome browser More...UCSCⁱ	uc003edt.4 human [O95168-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O95168	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		GORGO		129	UniRef100_O95168
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		PANTR		129
Uncharacterized protein		PANPA		129
UPI000012FDAD		HUMAN		128
NADH dehydrogenase (Ubiquinone) 1 beta subcomplex, 4, 15kDa		PANTR		129
O95168-2	NADH dehydrogenase (Ubiquinone) 1 beta subcomplex, 4, 15kDa		PANTR		120	UniRef100_K7AHK0

Protein	Similar proteins		Species	Score	Length	Source
O95168	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		GORGO		129	UniRef90_O95168
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		PANTR		129
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		PONAB		129
Uncharacterized protein		PANPA		129
Uncharacterized protein		NOMLE		129
+7

Protein	Similar proteins		Species	Score	Length	Source
O95168	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		GORGO		129	UniRef50_O95168
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		PANTR		129
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4		PONAB		129
Uncharacterized protein		PANPA		129
Uncharacterized protein		NOMLE		129
+119

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF044957 mRNA Translation: AAD05421.1 AC126182 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79525.1 BC000855 mRNA Translation: AAH00855.1 BC070285 mRNA Translation: AAH70285.1 BC146885 mRNA Translation: AAI46886.1 BC146887 mRNA Translation: AAI46888.1 BC146927 mRNA Translation: AAI46928.1 BC146932 mRNA Translation: AAI46933.1
CCDSⁱ	CCDS2999.1 [O95168-1] CCDS54630.1 [O95168-2]
PIRⁱ	JE0383
RefSeqⁱ	NP_001161803.1, NM_001168331.1 [O95168-2] NP_004538.2, NM_004547.5 [O95168-1]
UniGeneⁱ	Hs.304613

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5XTC	electron microscopy	3.70	o	2-129	[»]
	5XTD	electron microscopy	3.70	o	2-129	[»]
	5XTH	electron microscopy	3.90	o	2-129	[»]
	5XTI	electron microscopy	17.40	Bo/o	2-129	[»]
ProteinModelPortalⁱ	O95168
SMRⁱ	O95168
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	110790, 46 interactors
ComplexPortalⁱ	CPX-577 Mitochondrial respiratory chain complex I
CORUMⁱ	O95168
IntActⁱ	O95168, 30 interactors
MINTⁱ	O95168
STRINGⁱ	9606.ENSP00000184266

Chemistry databases

ChEMBLⁱ	CHEMBL2363065
DrugBankⁱ	DB00157 NADH

PTM databases

iPTMnetⁱ	O95168
PhosphoSitePlusⁱ	O95168
SwissPalmⁱ	O95168

Polymorphism and mutation databases

BioMutaⁱ	NDUFB4

BioMutaⁱ

NDUFB4

Proteomic databases

EPDⁱ	O95168
MaxQBⁱ	O95168
PaxDbⁱ	O95168
PeptideAtlasⁱ	O95168
PRIDEⁱ	O95168
ProteomicsDBⁱ	50681 50682 [O95168-2]
TopDownProteomicsⁱ	O95168-1 [O95168-1]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	4710
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000184266; ENSP00000184266; ENSG00000065518 [O95168-1] ENST00000485064; ENSP00000419578; ENSG00000065518 [O95168-2]
GeneIDⁱ	4710
KEGGⁱ	hsa:4710
UCSCⁱ	uc003edt.4 human [O95168-1]

Organism-specific databases

CTDⁱ	4710
EuPathDBⁱ	HostDB:ENSG00000065518.7
GeneCardsⁱ	NDUFB4
HGNCⁱ	HGNC:7699 NDUFB4
HPAⁱ	HPA051739
MIMⁱ	603840 gene
neXtProtⁱ	NX_O95168
OpenTargetsⁱ	ENSG00000065518
PharmGKBⁱ	PA31510
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IZUV Eukaryota ENOG4112CXK LUCA
GeneTreeⁱ	ENSGT00390000007133
HOGENOMⁱ	HOG000060241
HOVERGENⁱ	HBG001159
InParanoidⁱ	O95168
KOⁱ	K03960
OMAⁱ	TRWTYAR
OrthoDBⁱ	EOG091G1578
PhylomeDBⁱ	O95168
TreeFamⁱ	TF328761

Enzyme and pathway databases

Reactomeⁱ	R-HSA-611105 Respiratory electron transport R-HSA-6799198 Complex I biogenesis

Reactomeⁱ

R-HSA-611105 Respiratory electron transport
R-HSA-6799198 Complex I biogenesis

Miscellaneous databases

ChiTaRSⁱ	NDUFB4 human
GenomeRNAiⁱ	4710
Protein Ontology More...PROⁱ	PR:O95168
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000065518 Expressed in 237 organ(s), highest expression level in heart right ventricle
CleanExⁱ	HS_NDUFB4
ExpressionAtlasⁱ	O95168 baseline and differential
Genevisibleⁱ	O95168 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR009866 NADH_UbQ_OxRdtase_NDUFB4_su
PANTHERⁱ	PTHR15469 PTHR15469, 1 hit
Pfamⁱ	View protein in Pfam PF07225 NDUF_B4, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NDUB4_HUMAN
Accessionⁱ	O95168Primary (citable) accession number: O95168 Secondary accession number(s): B2RUY3, B9EJC7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - O95168 (NDUB4_HUMAN)

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NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

Nucleus

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ