UniProtKB - O95168 (NDUB4_HUMAN)
Protein
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
Gene
NDUFB4
Organism
Homo sapiens (Human)
Status
Functioni
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.1 Publication
GO - Molecular functioni
- NADH dehydrogenase (ubiquinone) activity Source: UniProtKB
GO - Biological processi
- mitochondrial electron transport, NADH to ubiquinone Source: Reactome
- mitochondrial respiratory chain complex I assembly Source: UniProtKB
- response to oxidative stress Source: UniProtKB
Keywordsi
Biological process | Electron transport, Respiratory chain, Transport |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00843-MONOMER |
PathwayCommonsi | O95168 |
Reactomei | R-HSA-611105, Respiratory electron transport R-HSA-6799198, Complex I biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4Alternative name(s): Complex I-B15 Short name: CI-B15 NADH-ubiquinone oxidoreductase B15 subunit |
Gene namesi | Name:NDUFB4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7699, NDUFB4 |
MIMi | 603840, gene |
neXtProti | NX_O95168 |
VEuPathDBi | HostDB:ENSG00000065518.7 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Single-pass membrane protein Sequence analysis; Matrix side Curated
Mitochondrion
- mitochondrial inner membrane Source: Reactome
- mitochondrial respiratory chain complex I Source: UniProtKB
- mitochondrion Source: HPA
Nucleus
- nuclear membrane Source: HPA
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 88 – 105 | HelicalSequence analysisAdd BLAST | 18 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000065518 |
PharmGKBi | PA31510 |
Miscellaneous databases
Pharosi | O95168, Tclin |
Chemistry databases
ChEMBLi | CHEMBL2363065 |
DrugBanki | DB00157, NADH |
DrugCentrali | O95168 |
Genetic variation databases
BioMutai | NDUFB4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000118800 | 2 – 129 | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4Add BLAST | 128 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 26 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O95168 |
jPOSTi | O95168 |
MassIVEi | O95168 |
MaxQBi | O95168 |
PaxDbi | O95168 |
PeptideAtlasi | O95168 |
PRIDEi | O95168 |
ProteomicsDBi | 50681 [O95168-1] 50682 [O95168-2] |
TopDownProteomicsi | O95168-1 [O95168-1] |
PTM databases
iPTMneti | O95168 |
PhosphoSitePlusi | O95168 |
SwissPalmi | O95168 |
Expressioni
Gene expression databases
Bgeei | ENSG00000065518, Expressed in heart right ventricle and 249 other tissues |
ExpressionAtlasi | O95168, baseline and differential |
Genevisiblei | O95168, HS |
Organism-specific databases
HPAi | ENSG00000065518, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Complex I is composed of 45 different subunits.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 110790, 58 interactors |
ComplexPortali | CPX-577, Mitochondrial respiratory chain complex I |
CORUMi | O95168 |
IntActi | O95168, 34 interactors |
MINTi | O95168 |
STRINGi | 9606.ENSP00000184266 |
Chemistry databases
BindingDBi | O95168 |
Miscellaneous databases
RNActi | O95168, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the complex I NDUFB4 subunit family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S2HF, Eukaryota |
GeneTreei | ENSGT00390000007133 |
HOGENOMi | CLU_123402_0_0_1 |
InParanoidi | O95168 |
OMAi | PLFFWFY |
OrthoDBi | 1434967at2759 |
PhylomeDBi | O95168 |
TreeFami | TF328761 |
Family and domain databases
InterProi | View protein in InterPro IPR009866, NADH_UbQ_OxRdtase_NDUFB4_su |
PANTHERi | PTHR15469, PTHR15469, 1 hit |
Pfami | View protein in Pfam PF07225, NDUF_B4, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O95168-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ
60 70 80 90 100
YNDPNRRGLI ENPALLRWAY ARTINVYPNF RPTPKNSLMG ALCGFGPLIF
110 120
IYYIIKTERD RKEKLIQEGK LDRTFHLSY
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JXQ9 | C9JXQ9_HUMAN | Complex I-B15 | NDUFB4 | 80 | Annotation score: | ||
F2Z3P9 | F2Z3P9_HUMAN | Complex I-B15 | NDUFB4 | 69 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042719 | 110 – 129 | DRKEK…FHLSY → VSIQTRCLVFE in isoform 2. 1 PublicationAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF044957 mRNA Translation: AAD05421.1 AC126182 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79525.1 BC000855 mRNA Translation: AAH00855.1 BC070285 mRNA Translation: AAH70285.1 BC146885 mRNA Translation: AAI46886.1 BC146887 mRNA Translation: AAI46888.1 BC146927 mRNA Translation: AAI46928.1 BC146932 mRNA Translation: AAI46933.1 |
CCDSi | CCDS2999.1 [O95168-1] CCDS54630.1 [O95168-2] |
PIRi | JE0383 |
RefSeqi | NP_001161803.1, NM_001168331.1 [O95168-2] NP_004538.2, NM_004547.5 [O95168-1] |
Genome annotation databases
Ensembli | ENST00000184266; ENSP00000184266; ENSG00000065518 [O95168-1] ENST00000485064; ENSP00000419578; ENSG00000065518 [O95168-2] |
GeneIDi | 4710 |
KEGGi | hsa:4710 |
UCSCi | uc003edt.4, human [O95168-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF044957 mRNA Translation: AAD05421.1 AC126182 Genomic DNA No translation available. CH471052 Genomic DNA Translation: EAW79525.1 BC000855 mRNA Translation: AAH00855.1 BC070285 mRNA Translation: AAH70285.1 BC146885 mRNA Translation: AAI46886.1 BC146887 mRNA Translation: AAI46888.1 BC146927 mRNA Translation: AAI46928.1 BC146932 mRNA Translation: AAI46933.1 |
CCDSi | CCDS2999.1 [O95168-1] CCDS54630.1 [O95168-2] |
PIRi | JE0383 |
RefSeqi | NP_001161803.1, NM_001168331.1 [O95168-2] NP_004538.2, NM_004547.5 [O95168-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5XTC | electron microscopy | 3.70 | o | 2-129 | [»] | |
5XTD | electron microscopy | 3.70 | o | 2-129 | [»] | |
5XTH | electron microscopy | 3.90 | o | 2-129 | [»] | |
5XTI | electron microscopy | 17.40 | Bo/o | 2-129 | [»] | |
SMRi | O95168 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110790, 58 interactors |
ComplexPortali | CPX-577, Mitochondrial respiratory chain complex I |
CORUMi | O95168 |
IntActi | O95168, 34 interactors |
MINTi | O95168 |
STRINGi | 9606.ENSP00000184266 |
Chemistry databases
BindingDBi | O95168 |
ChEMBLi | CHEMBL2363065 |
DrugBanki | DB00157, NADH |
DrugCentrali | O95168 |
PTM databases
iPTMneti | O95168 |
PhosphoSitePlusi | O95168 |
SwissPalmi | O95168 |
Genetic variation databases
BioMutai | NDUFB4 |
Proteomic databases
EPDi | O95168 |
jPOSTi | O95168 |
MassIVEi | O95168 |
MaxQBi | O95168 |
PaxDbi | O95168 |
PeptideAtlasi | O95168 |
PRIDEi | O95168 |
ProteomicsDBi | 50681 [O95168-1] 50682 [O95168-2] |
TopDownProteomicsi | O95168-1 [O95168-1] |
Protocols and materials databases
Antibodypediai | 32798, 81 antibodies |
DNASUi | 4710 |
Genome annotation databases
Ensembli | ENST00000184266; ENSP00000184266; ENSG00000065518 [O95168-1] ENST00000485064; ENSP00000419578; ENSG00000065518 [O95168-2] |
GeneIDi | 4710 |
KEGGi | hsa:4710 |
UCSCi | uc003edt.4, human [O95168-1] |
Organism-specific databases
CTDi | 4710 |
GeneCardsi | NDUFB4 |
HGNCi | HGNC:7699, NDUFB4 |
HPAi | ENSG00000065518, Tissue enhanced (skeletal) |
MIMi | 603840, gene |
neXtProti | NX_O95168 |
OpenTargetsi | ENSG00000065518 |
PharmGKBi | PA31510 |
VEuPathDBi | HostDB:ENSG00000065518.7 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S2HF, Eukaryota |
GeneTreei | ENSGT00390000007133 |
HOGENOMi | CLU_123402_0_0_1 |
InParanoidi | O95168 |
OMAi | PLFFWFY |
OrthoDBi | 1434967at2759 |
PhylomeDBi | O95168 |
TreeFami | TF328761 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00843-MONOMER |
PathwayCommonsi | O95168 |
Reactomei | R-HSA-611105, Respiratory electron transport R-HSA-6799198, Complex I biogenesis |
Miscellaneous databases
BioGRID-ORCSi | 4710, 366 hits in 879 CRISPR screens |
ChiTaRSi | NDUFB4, human |
GenomeRNAii | 4710 |
Pharosi | O95168, Tclin |
PROi | PR:O95168 |
RNActi | O95168, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000065518, Expressed in heart right ventricle and 249 other tissues |
ExpressionAtlasi | O95168, baseline and differential |
Genevisiblei | O95168, HS |
Family and domain databases
InterProi | View protein in InterPro IPR009866, NADH_UbQ_OxRdtase_NDUFB4_su |
PANTHERi | PTHR15469, PTHR15469, 1 hit |
Pfami | View protein in Pfam PF07225, NDUF_B4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NDUB4_HUMAN | |
Accessioni | O95168Primary (citable) accession number: O95168 Secondary accession number(s): B2RUY3, B9EJC7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families