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Protein

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Gene

NDUFB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.1 Publication

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: UniProtKB

GO - Biological processi

  • mitochondrial electron transport, NADH to ubiquinone Source: UniProtKB
  • mitochondrial respiratory chain complex I assembly Source: UniProtKB
  • response to oxidative stress Source: UniProtKB

Keywordsi

Biological processElectron transport, Respiratory chain, Transport

Enzyme and pathway databases

ReactomeiR-HSA-611105 Respiratory electron transport
R-HSA-6799198 Complex I biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
Alternative name(s):
Complex I-B15
Short name:
CI-B15
NADH-ubiquinone oxidoreductase B15 subunit
Gene namesi
Name:NDUFB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000065518.7
HGNCiHGNC:7699 NDUFB4
MIMi603840 gene
neXtProtiNX_O95168

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei88 – 105HelicalSequence analysisAdd BLAST18

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000065518
PharmGKBiPA31510

Chemistry databases

ChEMBLiCHEMBL2363065
DrugBankiDB00157 NADH

Polymorphism and mutation databases

BioMutaiNDUFB4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001188002 – 129NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4Add BLAST128

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei26PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO95168
MaxQBiO95168
PaxDbiO95168
PeptideAtlasiO95168
PRIDEiO95168
ProteomicsDBi50681
50682 [O95168-2]
TopDownProteomicsiO95168-1 [O95168-1]

PTM databases

iPTMnetiO95168
PhosphoSitePlusiO95168
SwissPalmiO95168

Expressioni

Gene expression databases

BgeeiENSG00000065518 Expressed in 237 organ(s), highest expression level in heart right ventricle
CleanExiHS_NDUFB4
ExpressionAtlasiO95168 baseline and differential
GenevisibleiO95168 HS

Organism-specific databases

HPAiHPA051739

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.2 Publications

Protein-protein interaction databases

BioGridi110790, 46 interactors
ComplexPortaliCPX-577 Mitochondrial respiratory chain complex I
CORUMiO95168
IntActiO95168, 30 interactors
MINTiO95168
STRINGi9606.ENSP00000184266

Structurei

3D structure databases

ProteinModelPortaliO95168
SMRiO95168
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFB4 subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZUV Eukaryota
ENOG4112CXK LUCA
GeneTreeiENSGT00390000007133
HOGENOMiHOG000060241
HOVERGENiHBG001159
InParanoidiO95168
KOiK03960
OMAiTRWTYAR
OrthoDBiEOG091G1578
PhylomeDBiO95168
TreeFamiTF328761

Family and domain databases

InterProiView protein in InterPro
IPR009866 NADH_UbQ_OxRdtase_NDUFB4_su
PANTHERiPTHR15469 PTHR15469, 1 hit
PfamiView protein in Pfam
PF07225 NDUF_B4, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O95168-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ
60 70 80 90 100
YNDPNRRGLI ENPALLRWAY ARTINVYPNF RPTPKNSLMG ALCGFGPLIF
110 120
IYYIIKTERD RKEKLIQEGK LDRTFHLSY
Length:129
Mass (Da):15,209
Last modified:January 23, 2007 - v3
Checksum:iE5060D0BF46FFF28
GO
Isoform 2 (identifier: O95168-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-129: DRKEKLIQEGKLDRTFHLSY → VSIQTRCLVFE

Note: No experimental confirmation available.
Show »
Length:120
Mass (Da):14,026
Checksum:i08BB084685E3098E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JXQ9C9JXQ9_HUMAN
NADH dehydrogenase [ubiquinone] 1 b...
NDUFB4
80Annotation score:
F2Z3P9F2Z3P9_HUMAN
NADH dehydrogenase [ubiquinone] 1 b...
NDUFB4
69Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042719110 – 129DRKEK…FHLSY → VSIQTRCLVFE in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044957 mRNA Translation: AAD05421.1
AC126182 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW79525.1
BC000855 mRNA Translation: AAH00855.1
BC070285 mRNA Translation: AAH70285.1
BC146885 mRNA Translation: AAI46886.1
BC146887 mRNA Translation: AAI46888.1
BC146927 mRNA Translation: AAI46928.1
BC146932 mRNA Translation: AAI46933.1
CCDSiCCDS2999.1 [O95168-1]
CCDS54630.1 [O95168-2]
PIRiJE0383
RefSeqiNP_001161803.1, NM_001168331.1 [O95168-2]
NP_004538.2, NM_004547.5 [O95168-1]
UniGeneiHs.304613

Genome annotation databases

EnsembliENST00000184266; ENSP00000184266; ENSG00000065518 [O95168-1]
ENST00000485064; ENSP00000419578; ENSG00000065518 [O95168-2]
GeneIDi4710
KEGGihsa:4710
UCSCiuc003edt.4 human [O95168-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044957 mRNA Translation: AAD05421.1
AC126182 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW79525.1
BC000855 mRNA Translation: AAH00855.1
BC070285 mRNA Translation: AAH70285.1
BC146885 mRNA Translation: AAI46886.1
BC146887 mRNA Translation: AAI46888.1
BC146927 mRNA Translation: AAI46928.1
BC146932 mRNA Translation: AAI46933.1
CCDSiCCDS2999.1 [O95168-1]
CCDS54630.1 [O95168-2]
PIRiJE0383
RefSeqiNP_001161803.1, NM_001168331.1 [O95168-2]
NP_004538.2, NM_004547.5 [O95168-1]
UniGeneiHs.304613

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XTCelectron microscopy3.70o2-129[»]
5XTDelectron microscopy3.70o2-129[»]
5XTHelectron microscopy3.90o2-129[»]
5XTIelectron microscopy17.40Bo/o2-129[»]
ProteinModelPortaliO95168
SMRiO95168
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110790, 46 interactors
ComplexPortaliCPX-577 Mitochondrial respiratory chain complex I
CORUMiO95168
IntActiO95168, 30 interactors
MINTiO95168
STRINGi9606.ENSP00000184266

Chemistry databases

ChEMBLiCHEMBL2363065
DrugBankiDB00157 NADH

PTM databases

iPTMnetiO95168
PhosphoSitePlusiO95168
SwissPalmiO95168

Polymorphism and mutation databases

BioMutaiNDUFB4

Proteomic databases

EPDiO95168
MaxQBiO95168
PaxDbiO95168
PeptideAtlasiO95168
PRIDEiO95168
ProteomicsDBi50681
50682 [O95168-2]
TopDownProteomicsiO95168-1 [O95168-1]

Protocols and materials databases

DNASUi4710
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000184266; ENSP00000184266; ENSG00000065518 [O95168-1]
ENST00000485064; ENSP00000419578; ENSG00000065518 [O95168-2]
GeneIDi4710
KEGGihsa:4710
UCSCiuc003edt.4 human [O95168-1]

Organism-specific databases

CTDi4710
EuPathDBiHostDB:ENSG00000065518.7
GeneCardsiNDUFB4
HGNCiHGNC:7699 NDUFB4
HPAiHPA051739
MIMi603840 gene
neXtProtiNX_O95168
OpenTargetsiENSG00000065518
PharmGKBiPA31510
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IZUV Eukaryota
ENOG4112CXK LUCA
GeneTreeiENSGT00390000007133
HOGENOMiHOG000060241
HOVERGENiHBG001159
InParanoidiO95168
KOiK03960
OMAiTRWTYAR
OrthoDBiEOG091G1578
PhylomeDBiO95168
TreeFamiTF328761

Enzyme and pathway databases

ReactomeiR-HSA-611105 Respiratory electron transport
R-HSA-6799198 Complex I biogenesis

Miscellaneous databases

ChiTaRSiNDUFB4 human
GenomeRNAii4710
PROiPR:O95168
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065518 Expressed in 237 organ(s), highest expression level in heart right ventricle
CleanExiHS_NDUFB4
ExpressionAtlasiO95168 baseline and differential
GenevisibleiO95168 HS

Family and domain databases

InterProiView protein in InterPro
IPR009866 NADH_UbQ_OxRdtase_NDUFB4_su
PANTHERiPTHR15469 PTHR15469, 1 hit
PfamiView protein in Pfam
PF07225 NDUF_B4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNDUB4_HUMAN
AccessioniPrimary (citable) accession number: O95168
Secondary accession number(s): B2RUY3, B9EJC7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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