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Protein

Gamma-aminobutyric acid receptor-associated protein

Gene

GABARAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.1 Publication

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • microtubule binding Source: Ensembl
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processApoptosis, Autophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1632852 Macroautophagy
R-HSA-8854214 TBC/RABGAPs
SIGNORiO95166

Protein family/group databases

MoonDBiO95166 Predicted
TCDBi1.A.9.5.2 the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein
Alternative name(s):
GABA(A) receptor-associated protein
MM46
Gene namesi
Name:GABARAP
Synonyms:FLC3B
ORF Names:HT004
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000170296.9
HGNCiHGNC:4067 GABARAP
MIMi605125 gene
neXtProtiNX_O95166

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24K → Q: No effect on WDFY3-binding. Impaired WDFY3-binding, but no effect on SQSTM1-binding; when associated with H-25 and H-54. 1 Publication1
Mutagenesisi25Y → H: No effect on WDFY3-binding. Impaired WDFY3-binding, but no effect on SQSTM1-binding; when associated with Q-24 and H-54. 1 Publication1
Mutagenesisi49 – 50YL → AA: Inhibits interaction with TECPR2. 1 Publication2
Mutagenesisi54D → H: No effect on WDFY3-binding. Impaired WDFY3-binding, but no effect on SQSTM1-binding; when associated with Q-24 and H-25. 1 Publication1
Mutagenesisi67R → A: No effect on interaction with TECPR2. 1 Publication1
Mutagenesisi116G → A: Impairs localization at the autophagosomal membrane. 1 Publication1

Organism-specific databases

DisGeNETi11337
OpenTargetsiENSG00000170296
PharmGKBiPA28480

Polymorphism and mutation databases

BioMutaiGABARAP

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123631 – 116Gamma-aminobutyric acid receptor-associated proteinAdd BLAST116
PropeptideiPRO_0000423065117Removed in mature form1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi116Phosphatidylethanolamine amidated glycine1 Publication1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116 – 117Cleavage; by ATG4B2

Keywords - PTMi

Lipoprotein

Proteomic databases

EPDiO95166
PaxDbiO95166
PeptideAtlasiO95166
PRIDEiO95166
ProteomicsDBi50679

PTM databases

iPTMnetiO95166
PhosphoSitePlusiO95166

Miscellaneous databases

PMAP-CutDBiO95166

Expressioni

Tissue specificityi

Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.2 Publications

Gene expression databases

BgeeiENSG00000170296
CleanExiHS_GABARAP
ExpressionAtlasiO95166 baseline and differential
GenevisibleiO95166 HS

Organism-specific databases

HPAiHPA065883

Interactioni

Subunit structurei

Interacts with GPHN and NSF (By similarity). Interacts with ATG3, ATG7, ATG13 (PubMed:11096062, PubMed:11825910, PubMed:12507496, PubMed:23043107). Interacts with alpha- and beta-tubulin (PubMed:9892355, PubMed:11729197). Interacts with GABRG2 (PubMed:9892355, PubMed:11729197). Interacts with RB1CC1 (PubMed:23043107). Interacts with ULK1 (PubMed:11146101, PubMed:23043107). Interacts with CALR (PubMed:19154346). Interacts with DDX47 (PubMed:15977068). Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22421968, PubMed:22470510). Interacts with TBC1D5 and TBC1D25 (PubMed:21383079, PubMed:22354992). Directly interacts with SQSTM1 (PubMed:17580304, PubMed:24668264). Interacts with MAPK15 (PubMed:22948227). Interacts with TECPR2 (PubMed:20562859). Interacts with PCM1 (PubMed:24089205). Interacts with TRIM5 and TRIM21 (PubMed:25127057, PubMed:26347139). Interacts with MEFV (PubMed:26347139). Interacts with KIF21B (By similarity). Interacts with WDFY3; this interaction is required for WDFY3 recruitment to MAP1LC3B-positive p62/SQSTM1 bodies (PubMed:24668264).By similarity21 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • GABA receptor binding Source: UniProtKB
  • microtubule binding Source: Ensembl
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116465, 65 interactors
DIPiDIP-35050N
ELMiO95166
IntActiO95166, 483 interactors
MINTiO95166
STRINGi9606.ENSP00000306866

Structurei

Secondary structure

1117
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Helixi11 – 24Combined sources14
Beta strandi28 – 35Combined sources8
Beta strandi47 – 52Combined sources6
Helixi57 – 68Combined sources12
Beta strandi77 – 80Combined sources4
Beta strandi87 – 90Combined sources4
Helixi91 – 98Combined sources8
Beta strandi101 – 103Combined sources3
Beta strandi105 – 111Combined sources7

3D structure databases

ProteinModelPortaliO95166
SMRiO95166
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95166

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 22Interaction with beta-tubulinAdd BLAST22
Regioni36 – 117Interaction with GPHNBy similarityAdd BLAST82
Regioni36 – 68Interaction with GABRG2Sequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654 Eukaryota
ENOG4111JAT LUCA
GeneTreeiENSGT00390000012937
HOGENOMiHOG000232034
HOVERGENiHBG051706
KOiK08341
OMAiVIPQTMT
OrthoDBiEOG091G10QX
PhylomeDBiO95166
TreeFamiTF314556

Family and domain databases

CDDicd01611 GABARAP, 1 hit
InterProiView protein in InterPro
IPR004241 Atg8-like
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10969 PTHR10969, 1 hit
PfamiView protein in Pfam
PF02991 Atg8, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL
60 70 80 90 100
VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE
110
EDFFLYIAYS DESVYGL
Length:117
Mass (Da):13,918
Last modified:May 1, 1999 - v1
Checksum:iBC0B84B8A51C1E32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161586 mRNA Translation: AAD47641.1
AB030711 mRNA Translation: BAB21549.1
AF044671 mRNA Translation: AAD02337.1
AF067171 mRNA Translation: AAD32455.1
AF183425 mRNA Translation: AAG09694.1
CCDSiCCDS11092.1
RefSeqiNP_009209.1, NM_007278.1
UniGeneiHs.647421

Genome annotation databases

EnsembliENST00000302386; ENSP00000306866; ENSG00000170296
GeneIDi11337
KEGGihsa:11337

Similar proteinsi

Entry informationi

Entry nameiGBRAP_HUMAN
AccessioniPrimary (citable) accession number: O95166
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 1999
Last modified: July 18, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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