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UniProtKB - O95154 (ARK73_HUMAN)
Protein
Aflatoxin B1 aldehyde reductase member 3
Gene
AKR7A3
Organism
Homo sapiens (Human)
Status
Functioni
Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 44 | NADP1 Publication | 1 | |
Active sitei | 49 | Proton donorBy similarity | 1 | |
Sitei | 77 | Lowers pKa of active site TyrBy similarity | 1 | |
Binding sitei | 113 | SubstrateBy similarity | 1 | |
Binding sitei | 169 | NADPBy similarity | 1 | |
Binding sitei | 222 | NADP1 Publication | 1 | |
Binding sitei | 232 | SubstrateBy similarity | 1 | |
Binding sitei | 235 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 143 – 144 | NADP1 Publication | 2 | |
Nucleotide bindingi | 198 – 208 | NADP1 PublicationAdd BLAST | 11 | |
Nucleotide bindingi | 290 – 298 | NADP1 Publication | 9 |
GO - Molecular functioni
- aldo-keto reductase (NADP) activity Source: GO_Central
- D-threo-aldose 1-dehydrogenase activity Source: InterPro
- electron transfer activity Source: UniProtKB
- identical protein binding Source: IntAct
GO - Biological processi
- aflatoxin catabolic process Source: Ensembl
- cellular aldehyde metabolic process Source: ProtInc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD, NADP |
Enzyme and pathway databases
PathwayCommonsi | O95154 |
Reactomei | R-HSA-5423646, Aflatoxin activation and detoxification |
SABIO-RKi | O95154 |
SignaLinki | O95154 |
Names & Taxonomyi
Protein namesi | Recommended name: Aflatoxin B1 aldehyde reductase member 3 (EC:1.-.-.-)Alternative name(s): AFB1 aldehyde reductase 2 Short name: AFB1-AR 2 |
Gene namesi | Name:AKR7A3 Synonyms:AFAR2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:390, AKR7A3 |
MIMi | 608477, gene |
neXtProti | NX_O95154 |
VEuPathDBi | HostDB:ENSG00000162482 |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 22977 |
OpenTargetsi | ENSG00000162482 |
PharmGKBi | PA24683 |
Miscellaneous databases
Pharosi | O95154, Tbio |
Genetic variation databases
BioMutai | AKR7A3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070378 | 1 – 331 | Aflatoxin B1 aldehyde reductase member 3Add BLAST | 331 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 6 | PhosphoserineBy similarity | 1 | |
Modified residuei | 85 | PhosphoserineCombined sources | 1 | |
Modified residuei | 227 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | O95154 |
MassIVEi | O95154 |
MaxQBi | O95154 |
PaxDbi | O95154 |
PeptideAtlasi | O95154 |
PRIDEi | O95154 |
ProteomicsDBi | 50669 |
PTM databases
iPTMneti | O95154 |
PhosphoSitePlusi | O95154 |
Expressioni
Tissue specificityi
Expressed in colon, kidney, liver, pancreas, adenocarcinoma and endometrium.2 Publications
Gene expression databases
Bgeei | ENSG00000162482, Expressed in body of stomach and 214 other tissues |
ExpressionAtlasi | O95154, baseline and differential |
Genevisiblei | O95154, HS |
Organism-specific databases
HPAi | ENSG00000162482, Tissue enhanced (intestine, liver, pancreas, stomach) |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
O95154
With | #Exp. | IntAct |
---|---|---|
AKR7A2 [O43488] | 6 | EBI-748869,EBI-748855 |
itself | 6 | EBI-748869,EBI-748869 |
KRT31 [Q15323] | 3 | EBI-748869,EBI-948001 |
KRTAP1-1 [Q07627] | 3 | EBI-748869,EBI-11959885 |
TERF2IP [Q9NYB0] | 2 | EBI-748869,EBI-750109 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 116626, 16 interactors |
IntActi | O95154, 12 interactors |
STRINGi | 9606.ENSP00000355377 |
Miscellaneous databases
RNActi | O95154, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O95154 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O95154 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QU2T, Eukaryota |
GeneTreei | ENSGT00940000163704 |
HOGENOMi | CLU_023205_1_1_1 |
InParanoidi | O95154 |
OMAi | QKRGIVM |
OrthoDBi | 1226539at2759 |
PhylomeDBi | O95154 |
TreeFami | TF329173 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
i Sequence
Sequence statusi: Complete.
O95154-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFVYS
60 70 80 90 100
EGQSETILGG LGLRLGGSDC RVKIDTKAIP LFGNSLKPDS LRFQLETSLK
110 120 130 140 150
RLQCPRVDLF YLHMPDHSTP VEETLRACHQ LHQEGKFVEL GLSNYAAWEV
160 170 180 190 200
AEICTLCKSN GWILPTVYQG MYNAITRQVE TELFPCLRHF GLRFYAFNPL
210 220 230 240 250
AGGLLTGKYK YEDKNGKQPV GRFFGNTWAE MYRNRYWKEH HFEGIALVEK
260 270 280 290 300
ALQAAYGASA PSMTSATLRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA
310 320 330
AAEEGPLEPA VVDAFNQAWH LVTHECPNYF R
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | E → D in AAD02195 (PubMed:10383892).Curated | 1 | |
Sequence conflicti | 247 | L → P in AAH42420 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_017416 | 138 | V → M2 PublicationsCorresponds to variant dbSNP:rs2231198Ensembl. | 1 | |
Natural variantiVAR_017417 | 215 | N → D3 PublicationsCorresponds to variant dbSNP:rs1738023Ensembl. | 1 | |
Natural variantiVAR_017418 | 323 | T → A3 PublicationsCorresponds to variant dbSNP:rs1738025Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF040639 mRNA Translation: AAD02195.1 AJ271799 mRNA Translation: CAC81076.1 AL035413 Genomic DNA No translation available. BC025709 mRNA Translation: AAH25709.1 BC031562 mRNA Translation: AAH31562.1 BC042420 mRNA Translation: AAH42420.2 |
CCDSi | CCDS193.1 |
RefSeqi | NP_036199.2, NM_012067.2 |
Genome annotation databases
Ensembli | ENST00000361640; ENSP00000355377; ENSG00000162482 |
GeneIDi | 22977 |
KEGGi | hsa:22977 |
MANE-Selecti | ENST00000361640.5; ENSP00000355377.4; NM_012067.3; NP_036199.2 |
UCSCi | uc001bbv.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF040639 mRNA Translation: AAD02195.1 AJ271799 mRNA Translation: CAC81076.1 AL035413 Genomic DNA No translation available. BC025709 mRNA Translation: AAH25709.1 BC031562 mRNA Translation: AAH31562.1 BC042420 mRNA Translation: AAH42420.2 |
CCDSi | CCDS193.1 |
RefSeqi | NP_036199.2, NM_012067.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CLP | X-ray | 3.00 | A/B/C/D/E/F/G/H/I/J/K | 8-331 | [»] | |
SMRi | O95154 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116626, 16 interactors |
IntActi | O95154, 12 interactors |
STRINGi | 9606.ENSP00000355377 |
PTM databases
iPTMneti | O95154 |
PhosphoSitePlusi | O95154 |
Genetic variation databases
BioMutai | AKR7A3 |
Proteomic databases
jPOSTi | O95154 |
MassIVEi | O95154 |
MaxQBi | O95154 |
PaxDbi | O95154 |
PeptideAtlasi | O95154 |
PRIDEi | O95154 |
ProteomicsDBi | 50669 |
Protocols and materials databases
Antibodypediai | 29611, 165 antibodies from 28 providers |
DNASUi | 22977 |
Genome annotation databases
Ensembli | ENST00000361640; ENSP00000355377; ENSG00000162482 |
GeneIDi | 22977 |
KEGGi | hsa:22977 |
MANE-Selecti | ENST00000361640.5; ENSP00000355377.4; NM_012067.3; NP_036199.2 |
UCSCi | uc001bbv.2, human |
Organism-specific databases
CTDi | 22977 |
DisGeNETi | 22977 |
GeneCardsi | AKR7A3 |
HGNCi | HGNC:390, AKR7A3 |
HPAi | ENSG00000162482, Tissue enhanced (intestine, liver, pancreas, stomach) |
MIMi | 608477, gene |
neXtProti | NX_O95154 |
OpenTargetsi | ENSG00000162482 |
PharmGKBi | PA24683 |
VEuPathDBi | HostDB:ENSG00000162482 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QU2T, Eukaryota |
GeneTreei | ENSGT00940000163704 |
HOGENOMi | CLU_023205_1_1_1 |
InParanoidi | O95154 |
OMAi | QKRGIVM |
OrthoDBi | 1226539at2759 |
PhylomeDBi | O95154 |
TreeFami | TF329173 |
Enzyme and pathway databases
PathwayCommonsi | O95154 |
Reactomei | R-HSA-5423646, Aflatoxin activation and detoxification |
SABIO-RKi | O95154 |
SignaLinki | O95154 |
Miscellaneous databases
BioGRID-ORCSi | 22977, 40 hits in 1001 CRISPR screens |
EvolutionaryTracei | O95154 |
GenomeRNAii | 22977 |
Pharosi | O95154, Tbio |
PROi | PR:O95154 |
RNActi | O95154, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000162482, Expressed in body of stomach and 214 other tissues |
ExpressionAtlasi | O95154, baseline and differential |
Genevisiblei | O95154, HS |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
SUPFAMi | SSF51430, SSF51430, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARK73_HUMAN | |
Accessioni | O95154Primary (citable) accession number: O95154 Secondary accession number(s): Q86SR4 Q9NUC3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | February 21, 2001 | |
Last modified: | February 23, 2022 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families