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Protein

Splicing factor, arginine/serine-rich 15

Gene

SCAF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act to physically and functionally link transcription and pre-mRNA processing.By similarity

GO - Molecular functioni

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor, arginine/serine-rich 15
Alternative name(s):
CTD-binding SR-like protein RA4
SR-related and CTD-associated factor 4
Gene namesi
Name:SCAF4
Synonyms:KIAA1172, SFRS15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000156304.14
HGNCiHGNC:19304 SCAF4
MIMi616023 gene
neXtProtiNX_O95104

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi57466
OpenTargetsiENSG00000156304
PharmGKBiPA134903281

Polymorphism and mutation databases

BioMutaiSCAF4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819431 – 1147Splicing factor, arginine/serine-rich 15Add BLAST1147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49N6-acetyllysineCombined sources1
Modified residuei154PhosphoserineCombined sources1
Modified residuei656PhosphoserineCombined sources1
Modified residuei1004PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO95104
MaxQBiO95104
PaxDbiO95104
PeptideAtlasiO95104
PRIDEiO95104
ProteomicsDBi50653
50654 [O95104-2]

PTM databases

iPTMnetiO95104
PhosphoSitePlusiO95104

Expressioni

Gene expression databases

BgeeiENSG00000156304
CleanExiHS_SFRS15
GenevisibleiO95104 HS

Organism-specific databases

HPAiHPA018319
HPA018668
HPA021504
HPA028807

Interactioni

Subunit structurei

Interacts with the repetitive C-terminal domain (CTD) of RNA polymerase II.By similarity

Protein-protein interaction databases

BioGridi121536, 38 interactors
IntActiO95104, 5 interactors
MINTiO95104
STRINGi9606.ENSP00000286835

Structurei

3D structure databases

ProteinModelPortaliO95104
SMRiO95104
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 139CIDPROSITE-ProRule annotationAdd BLAST139
Domaini508 – 582RRMPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi155 – 158Poly-Pro4
Compositional biasi304 – 311Poly-Ala8
Compositional biasi313 – 316Poly-Pro4
Compositional biasi716 – 723Poly-Pro8
Compositional biasi740 – 746Poly-Pro7
Compositional biasi957 – 966Poly-Gln10

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated

Phylogenomic databases

eggNOGiKOG0132 Eukaryota
ENOG410XT48 LUCA
GeneTreeiENSGT00530000063946
HOGENOMiHOG000143432
HOVERGENiHBG055644
InParanoidiO95104
KOiK13167
OMAiQDPVHHQ
OrthoDBiEOG091G0377
PhylomeDBiO95104
TreeFamiTF324527

Family and domain databases

CDDicd12461 RRM_SCAF4, 1 hit
Gene3Di1.25.40.90, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR006569 CID_dom
IPR008942 ENTH_VHS
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR006903 RNA_pol_II-bd
IPR000504 RRM_dom
IPR034369 SCAF4_RRM
PfamiView protein in Pfam
PF04818 CTD_bind, 1 hit
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00582 RPR, 1 hit
SM00360 RRM, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS51391 CID, 1 hit
PS50102 RRM, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95104-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAVNAFNQE LFSLMDMKPP ISRAKMILIT KAAIKAIKLY KHVVQIVEKF
60 70 80 90 100
IKKCKPEYKV PGLYVIDSIV RQSRHQFGTD KDVFGPRFSK NITATFQYLY
110 120 130 140 150
LCPSEDKSKI VRVLNLWQKN GVFKIEIIQP LLDMAAGTSN AAPVAENVTN
160 170 180 190 200
NEGSPPPPVK VSSEPPTQAT PNSVPAVPQL PSSDAFAAVA QLFQTTQGQQ
210 220 230 240 250
LQQILQTFQQ PPKPQSPALD NAVMAQVQAI TAQLKTTPTQ PSEQKAAFPP
260 270 280 290 300
PEQKTAFDKK LLDRFDYDDE PEAVEESKKE DTTAVTTTAP AAAVPPAPTA
310 320 330 340 350
TVPAAAAPAA ASPPPPQAPF GFPGDGMQQP AYTQHQNMDQ FQPRMMGIQQ
360 370 380 390 400
DPMHHQVPLP PNGQMPGFGL LPTPPFPPMA QPVIPPTPPV QQPFQASFQA
410 420 430 440 450
QNEPLTQKPH QQEMEVEQPC IQEVKRHMSD NRKSRSRSAS RSPKRRRSRS
460 470 480 490 500
GSRSRRSRHR RSRSRSRDRR RHSPRSRSQE RRDREKERER RQKGLPQVKP
510 520 530 540 550
ETASVCSTTL WVGQLDKRTT QQDVASLLEE FGPIESINMI PPRGCAYIVM
560 570 580 590 600
VHRQDAYRAL QKLSRGNYKV NQKSIKIAWA LNKGIKADYK QYWDVELGVT
610 620 630 640 650
YIPWDKVKPE ELESFCEGGM LDSDTLNPDW KGIPKKPENE VAQNGGAETS
660 670 680 690 700
HTEPVSPIPK PLPVPVPPIP VPAPITVPPP QVPPHQPGPP VVGALQPPAF
710 720 730 740 750
TPPLGIPPPG FGPGVPPPPP PPPFLRPGFN PMHLPPGFLP PGPPPPITPP
760 770 780 790 800
VSIPPPHTPP ISIPNSTIAG INEDTTKDLS IGNPIPTVVS GARGNAESGD
810 820 830 840 850
SVKMYGSAVP PAAPTNLPTP PVTQPVSLLG TQGVAPGPVI GLQAPSTGLL
860 870 880 890 900
GARPGLIPLQ RPPGMPPPHL QRFPLMPPRP MPPHMMHRGP PPGPGGFAMP
910 920 930 940 950
PPHGMKGPFP PHGPFVRPGG MPGLGGPGPG PGGPEDRDGR QQPPQQPQQQ
960 970 980 990 1000
PQPQAPQQPQ QQQQQQPPPS QQPPPTQQQP QQFRNDNRQQ FNSGRDQERF
1010 1020 1030 1040 1050
GRRSFGNRVE NDRERYGNRN DDRDNSNRDR REWGRRSPDR DRHRDLEERN
1060 1070 1080 1090 1100
RRSSGHRDRE RDSRDRESRR EKEEARGKEK PEVTDRAGGN KTVEPPISQV
1110 1120 1130 1140
GNVDTASELE KGVSEAAVLK PSEELPAEAT SSVEPEKDSG SAAEAPR
Length:1,147
Mass (Da):125,869
Last modified:February 15, 2005 - v3
Checksum:iDED5EEAB45DB4B83
GO
Isoform 2 (identifier: O95104-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     766-788: STIAGINEDTTKDLSIGNPIPTV → L

Note: No experimental confirmation available.
Show »
Length:1,125
Mass (Da):123,643
Checksum:iD771D62662D7B0C6
GO
Isoform 3 (identifier: O95104-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     38-52: Missing.

Note: No experimental confirmation available.
Show »
Length:1,132
Mass (Da):124,014
Checksum:iF19B820E11955748
GO

Sequence cautioni

The sequence AAD09327 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → W in AAD09327 (Ref. 1) Curated1
Sequence conflicti260 – 264Missing in CAB55911 (PubMed:17974005).Curated5
Sequence conflicti283T → I in AAD09327 (Ref. 1) Curated1
Sequence conflicti286T → A in AAD09327 (Ref. 1) Curated1
Sequence conflicti299T → A in AAD09327 (Ref. 1) Curated1
Sequence conflicti302V → L in BAA86486 (PubMed:10574461).Curated1
Sequence conflicti552H → Y in AAD09327 (Ref. 1) Curated1
Sequence conflicti560L → V in AAD09327 (Ref. 1) Curated1
Sequence conflicti571N → T (Ref. 1) Curated1
Sequence conflicti598 – 600GVT → CVI in AAD09327 (Ref. 1) Curated3
Sequence conflicti604 – 606WDK → CDN in AAD09327 (Ref. 1) Curated3
Sequence conflicti612L → M in AAD09327 (Ref. 1) Curated1
Sequence conflicti616C → Y in AAD09327 (Ref. 1) Curated1
Sequence conflicti624D → N in AK308406 (PubMed:17974005).Curated1
Sequence conflicti808 – 826AVPPA…VTQPV → TCCTHESAHPSCNPACLPC in AAD09327 (Ref. 1) CuratedAdd BLAST19
Sequence conflicti844A → E in AAD09327 (Ref. 1) Curated1
Sequence conflicti1139S → G (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052234846S → Y. Corresponds to variant dbSNP:rs12152067Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04735138 – 52Missing in isoform 3. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_005879766 – 788STIAG…PIPTV → L in isoform 2. 2 PublicationsAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023142 mRNA Translation: AAD09327.1 Different initiation.
AK308406 mRNA No translation available.
AL834304 mRNA Translation: CAD38974.2
AL117417 mRNA Translation: CAB55911.1 Sequence problems.
AP001711 Genomic DNA No translation available.
BC014921 mRNA Translation: AAH14921.1
BC052286 mRNA Translation: AAH52286.1
BC064990 mRNA Translation: AAH64990.1
AB032998 mRNA Translation: BAA86486.1
CCDSiCCDS33537.1 [O95104-1]
CCDS46644.1 [O95104-2]
CCDS54482.1 [O95104-3]
RefSeqiNP_001138916.1, NM_001145444.1 [O95104-3]
NP_001138917.1, NM_001145445.1 [O95104-2]
NP_065757.1, NM_020706.2 [O95104-1]
UniGeneiHs.17255

Genome annotation databases

EnsembliENST00000286835; ENSP00000286835; ENSG00000156304 [O95104-1]
ENST00000399804; ENSP00000382703; ENSG00000156304 [O95104-2]
ENST00000434667; ENSP00000402377; ENSG00000156304 [O95104-3]
GeneIDi57466
KEGGihsa:57466
UCSCiuc002ypd.3 human [O95104-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSFR15_HUMAN
AccessioniPrimary (citable) accession number: O95104
Secondary accession number(s): C9JLZ0
, Q0P5W8, Q6P1M5, Q8N3I8, Q9UFM1, Q9ULP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 15, 2005
Last modified: July 18, 2018
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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