GLCE

Functionⁱ

Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.1 Publication

Catalytic activityⁱ

Epimerization of D-glucuronate in heparosan-N-sulfate to L-iduronate.2 Publications

Pathwayⁱ: heparan sulfate biosynthesis

This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.

Pathwayⁱ: heparin biosynthesis

This protein is involved in the pathway heparin biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparin biosynthesis and in Glycan metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	180	SubstrateBy similarity	1
Siteⁱ	180	Critical for catalysisBy similarity	1
Siteⁱ	187	Critical for catalysisBy similarity	1
Binding siteⁱ	213	SubstrateBy similarity	1
Binding siteⁱ	514	SubstrateBy similarity	1
Siteⁱ	560	Critical for catalysisBy similarity	1
Binding siteⁱ	563	SubstrateBy similarity	1
Siteⁱ	578	Critical for catalysisBy similarity	1

GO - Molecular functionⁱ

heparosan-N-sulfate-glucuronate 5-epimerase activity
Source: HGNC
Inferred from direct assayⁱ
- 15853773
racemase and epimerase activity, acting on carbohydrates and derivatives Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

heparan sulfate proteoglycan biosynthetic process
Source: UniProtKB
Inferred from direct assayⁱ
- 20118238
heparin biosynthetic process Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Isomerase

Molecular function

Isomerase

Enzyme and pathway databases

BRENDAⁱ	5.1.3.17 2681
Reactomeⁱ	R-HSA-2022928 HS-GAG biosynthesis
UniPathwayⁱ	UPA00756 UPA00862

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: D-glucuronyl C5-epimerase (EC:5.1.3.172 Publications) Alternative name(s): Heparan sulfate C5-epimerase Short name: Hsepi Heparin/heparan sulfate:glucuronic acid C5-epimerase Heparosan-N-sulfate-glucuronate 5-epimerase
Gene namesⁱ	Name:GLCE Synonyms:KIAA0836Imported
Organismⁱ	Homo sapiens (Human)Imported
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 15

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000138604.9
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:17855 GLCE
MIMⁱ	612134 gene
neXtProtⁱ	NX_O94923

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 11	CytoplasmicSequence analysisAdd BLAST	11
Transmembraneⁱ	12 – 28	Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST	17
Topological domainⁱ	29 – 617	LumenalSequence analysisAdd BLAST	589

Keywords - Cellular componentⁱ

Golgi apparatus, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	146	Y → A: Reduces enzyme activity by about 60%. 1 Publication	1
Mutagenesisⁱ	162	Y → A: Reduces enzyme activity by about 75%. 1 Publication	1
Mutagenesisⁱ	168	Y → A: Almost abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	210	Y → A: Reduces enzyme activity by about 30%. 1 Publication	1
Mutagenesisⁱ	222	Y → A: Almost abolishes enzyme activity. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	26035
Open Targets More...OpenTargetsⁱ	ENSG00000138604
PharmGKBⁱ	PA145148750

Polymorphism and mutation databases

BioMutaⁱ	GLCE

BioMutaⁱ

GLCE

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000192645	1 – 617	D-glucuronyl C5-epimeraseAdd BLAST		617

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Glycosylationⁱ	225	N-linked (GlcNAc...) asparagine1 Publication		1

Keywords - PTMⁱ

Glycoprotein

Proteomic databases

EPDⁱ	O94923
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O94923
PaxDbⁱ	O94923
PeptideAtlas More...PeptideAtlasⁱ	O94923
PRIDEⁱ	O94923
ProteomicsDBⁱ	50559

PTM databases

iPTMnetⁱ	O94923
PhosphoSitePlusⁱ	O94923
SwissPalmⁱ	O94923

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000138604 Expressed in 194 organ(s), highest expression level in testis
CleanExⁱ	HS_GLCE
ExpressionAtlasⁱ	O94923 baseline and differential
Genevisibleⁱ	O94923 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA040481 HPA048216

HPAⁱ

HPA040481
HPA048216

Interactionⁱ

Subunit structureⁱ

Homodimer. Interacts with HS2ST1.By similarity

Protein-protein interaction databases

BioGridⁱ	117501, 15 interactors
IntActⁱ	O94923, 10 interactors
Molecular INTeraction database More...MINTⁱ	O94923
STRINGⁱ	9606.ENSP00000261858

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O94923
SMRⁱ	O94923
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	185 – 187	Substrate bindingBy similarity	3
Regionⁱ	429 – 432	Substrate bindingBy similarity	4
Regionⁱ	575 – 581	Substrate bindingBy similarity	7

Sequence similaritiesⁱ

Belongs to the D-glucuronyl C5-epimerase family.Curated

Keywords - Domainⁱ

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG3760 Eukaryota ENOG410XQJ3 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000006043
HOGENOMⁱ	HOG000230542
HOVERGENⁱ	HBG031576
InParanoidⁱ	O94923
KEGG Orthology (KO) More...KOⁱ	K01793
OMAⁱ	RGVFMYF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03C0
PhylomeDBⁱ	O94923
TreeFamⁱ	TF105869

Family and domain databases

InterProⁱ	View protein in InterPro IPR010598 C5-epim_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06662 C5-epim_C, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.ⁱShow all

O94923-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MRCLAARVNY KTLIIICALF TLVTVLLWNK CSSDKAIQFP RRSSSGFRVD 
        60         70         80         90        100
GFEKRAAASE SNNYMNHVAK QQSEEAFPQE QQKAPPVVGG FNSNVGSKVL 
       110        120        130        140        150
GLKYEEIDCL INDEHTIKGR REGNEVFLPF TWVEKYFDVY GKVVQYDGYD 
       160        170        180        190        200
RFEFSHSYSK VYAQRAPYHP DGVFMSFEGY NVEVRDRVKC ISGVEGVPLS 
       210        220        230        240        250
TQWGPQGYFY PIQIAQYGLS HYSKNLTEKP PHIEVYETAE DRDKNKPNDW 
       260        270        280        290        300
TVPKGCFMAN VADKSRFTNV KQFIAPETSE GVSLQLGNTK DFIISFDLKF 
       310        320        330        340        350
LTNGSVSVVL ETTEKNQLFT IHYVSNAQLI AFKERDIYYG IGPRTSWSTV 
       360        370        380        390        400
TRDLVTDLRK GVGLSNTKAV KPTKIMPKKV VRLIAKGKGF LDNITISTTA 
       410        420        430        440        450
HMAAFFAASD WLVRNQDEKG GWPIMVTRKL GEGFKSLEPG WYSAMAQGQA 
       460        470        480        490        500
ISTLVRAYLL TKDHIFLNSA LRATAPYKFL SEQHGVKAVF MNKHDWYEEY 
       510        520        530        540        550
PTTPSSFVLN GFMYSLIGLY DLKETAGEKL GKEARSLYER GMESLKAMLP 
       560        570        580        590        600
LYDTGSGTIY DLRHFMLGIA PNLARWDYHT THINQLQLLS TIDESPVFKE 
       610 
FVKRWKSYLK GSRAKHN

Length:617

Mass (Da):70,101

Last modified:June 16, 2009 - v3

Checksum:ⁱ2F909A15FEA6DA05

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	H0YNP1	H0YNP1_HUMAN		D-glucuronyl C5-epimerase D-glucuronyl C5-epimerase	GLCE	553	Annotation score:

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_055837	65	M → V. Corresponds to variant dbSNP:rs12440300Ensembl.		1
Natural variantⁱVAR_057958	597	V → I1 PublicationCorresponds to variant dbSNP:rs3865014Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY635582 mRNA Translation: AAT48654.1 AC026992 mRNA No translation available. AB020643 mRNA Translation: BAA74859.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS32277.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001311022.1, NM_001324093.1 NP_001311023.1, NM_001324094.1 NP_056369.1, NM_015554.2 XP_005254355.1, XM_005254298.3
UniGeneⁱ	Hs.183006

Genome annotation databases

Ensemblⁱ	ENST00000261858; ENSP00000261858; ENSG00000138604
GeneIDⁱ	26035
KEGGⁱ	hsa:26035
UCSC genome browser More...UCSCⁱ	uc002ary.2 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O94923	D-glucuronyl C5-epimerase		HUMAN		553	UniRef100_O94923
UPI0007DC5782		HUMAN		623
UPI0007B329A4		HUMAN		360

Protein	Similar proteins		Species	Score	Length	Source
O94923	D-glucuronyl C5-epimerase		BOVIN		617	UniRef90_O94923
D-glucuronyl C5-epimerase		HUMAN		553
D-glucuronyl C5-epimerase		ALLMI		751
GLCE isoform 2		PONAB		619
D-glucuronyl C5-epimerase		MACMU		618
+108

Protein	Similar proteins		Species	Score	Length	Source
O94923	D-glucuronyl C5-epimerase		MOUSE		618	UniRef50_Q9EPS3
D-glucuronyl C5-epimerase		BOVIN		617
D-glucuronyl C5-epimerase		CAMFR		749
Uncharacterized protein		RABIT		618
D-glucuronyl C5-epimerase		TUPCH		618
+211

Entry informationⁱ

Entry nameⁱ	GLCE_HUMAN
Accessionⁱ	O94923Primary (citable) accession number: O94923 Secondary accession number(s): Q6GUQ2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
	Last sequence update:	June 16, 2009
	Last modified:	September 12, 2018
	This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O94923 (GLCE_HUMAN)

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D-glucuronyl C5-epimerase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: heparan sulfate biosynthesis

Pathwayi: heparin biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Golgi apparatus

Golgi apparatus

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Pathwayⁱ: heparan sulfate biosynthesis

Pathwayⁱ: heparin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Computationally mapped potential isoform sequencesⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ