UniProtKB - O94923 (GLCE_HUMAN)
Protein
D-glucuronyl C5-epimerase
Gene
GLCE
Organism
Homo sapiens (Human)
Status
Functioni
Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.3 Publications
Catalytic activityi
- EC:5.1.3.173 Publications
: heparan sulfate biosynthesis Pathwayi
This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.2 PublicationsView all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.
Pathwayi: heparin biosynthesis
This protein is involved in the pathway heparin biosynthesis, which is part of Glycan metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway heparin biosynthesis and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 180 | SubstrateCombined sources1 Publication | 1 | |
Sitei | 180 | Critical for catalysisBy similarity | 1 | |
Sitei | 187 | Critical for catalysisBy similarity | 1 | |
Binding sitei | 202 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 210 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 213 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 216 | SubstrateCombined sources1 Publication | 1 | |
Metal bindingi | 238 | Calcium; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 240 | CalciumCombined sources1 Publication | 1 | |
Metal bindingi | 268 | CalciumCombined sources1 Publication | 1 | |
Metal bindingi | 269 | Calcium; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 392 | CalciumCombined sources1 Publication | 1 | |
Binding sitei | 510 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 514 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 560 | SubstrateCombined sources1 Publication | 1 | |
Sitei | 560 | Critical for catalysisBy similarity | 1 | |
Binding sitei | 563 | SubstrateCombined sources1 Publication | 1 | |
Sitei | 578 | Critical for catalysis1 Publication | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- heparosan-N-sulfate-glucuronate 5-epimerase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- racemase and epimerase activity, acting on carbohydrates and derivatives Source: UniProtKB
GO - Biological processi
- heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
- heparin biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Isomerase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 5.1.3.17, 2681 |
PathwayCommonsi | O94923 |
Reactomei | R-HSA-2022928, HS-GAG biosynthesis |
UniPathwayi | UPA00756 UPA00862 |
Names & Taxonomyi
Protein namesi | Recommended name: D-glucuronyl C5-epimerase (EC:5.1.3.173 Publications)Alternative name(s): Heparan sulfate C5-epimerase Short name: Hsepi Heparin/heparan sulfate:glucuronic acid C5-epimerase Heparosan-N-sulfate-glucuronate 5-epimerase |
Gene namesi | Name:GLCE Synonyms:KIAA0836Imported |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17855, GLCE |
MIMi | 612134, gene |
neXtProti | NX_O94923 |
VEuPathDBi | HostDB:ENSG00000138604.9 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 28 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 17 | |
Topological domaini | 29 – 617 | LumenalSequence analysisAdd BLAST | 589 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 146 | Y → A: Reduces enzyme activity by about 60%. 1 Publication | 1 | |
Mutagenesisi | 162 | Y → A: Reduces enzyme activity by about 75%. 1 Publication | 1 | |
Mutagenesisi | 168 | Y → A: Almost abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 210 | Y → A: Reduces enzyme activity by about 30%. 1 Publication | 1 | |
Mutagenesisi | 222 | Y → A: Almost abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 499 | E → Q: Loss of enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 500 | Y → F: Mildly decreased enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 578 | Y → F: Loss of enzyme activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 26035 |
OpenTargetsi | ENSG00000138604 |
PharmGKBi | PA145148750 |
Miscellaneous databases
Pharosi | O94923, Tbio |
Genetic variation databases
BioMutai | GLCE |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192645 | 1 – 617 | D-glucuronyl C5-epimeraseAdd BLAST | 617 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 225 | N-linked (GlcNAc...) asparagineCombined sources2 Publications | 1 | |
Glycosylationi | 303 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 393 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | O94923 |
jPOSTi | O94923 |
MassIVEi | O94923 |
MaxQBi | O94923 |
PaxDbi | O94923 |
PeptideAtlasi | O94923 |
PRIDEi | O94923 |
ProteomicsDBi | 50559 |
PTM databases
GlyGeni | O94923, 4 sites, 1 O-linked glycan (1 site) |
iPTMneti | O94923 |
PhosphoSitePlusi | O94923 |
SwissPalmi | O94923 |
Expressioni
Gene expression databases
Bgeei | ENSG00000138604, Expressed in testis and 206 other tissues |
ExpressionAtlasi | O94923, baseline and differential |
Genevisiblei | O94923, HS |
Organism-specific databases
HPAi | ENSG00000138604, Tissue enhanced (brain) |
Interactioni
Subunit structurei
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 117501, 27 interactors |
IntActi | O94923, 20 interactors |
MINTi | O94923 |
STRINGi | 9606.ENSP00000261858 |
Miscellaneous databases
RNActi | O94923, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O94923 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 185 – 187 | Substrate bindingCombined sources1 Publication | 3 | |
Regioni | 429 – 432 | Substrate bindingBy similarity | 4 | |
Regioni | 499 – 500 | Substrate bindingCombined sources1 Publication | 2 | |
Regioni | 572 – 581 | Substrate bindingCombined sources1 Publication | 10 |
Sequence similaritiesi
Belongs to the D-glucuronyl C5-epimerase family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3760, Eukaryota |
GeneTreei | ENSGT00390000006043 |
InParanoidi | O94923 |
OMAi | RGVFMYF |
OrthoDBi | 561819at2759 |
PhylomeDBi | O94923 |
TreeFami | TF105869 |
Family and domain databases
InterProi | View protein in InterPro IPR010598, C5-epim_C IPR039721, C5-epimerase |
PANTHERi | PTHR13174, PTHR13174, 1 hit |
Pfami | View protein in Pfam PF06662, C5-epim_C, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O94923-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRCLAARVNY KTLIIICALF TLVTVLLWNK CSSDKAIQFP RRSSSGFRVD
60 70 80 90 100
GFEKRAAASE SNNYMNHVAK QQSEEAFPQE QQKAPPVVGG FNSNVGSKVL
110 120 130 140 150
GLKYEEIDCL INDEHTIKGR REGNEVFLPF TWVEKYFDVY GKVVQYDGYD
160 170 180 190 200
RFEFSHSYSK VYAQRAPYHP DGVFMSFEGY NVEVRDRVKC ISGVEGVPLS
210 220 230 240 250
TQWGPQGYFY PIQIAQYGLS HYSKNLTEKP PHIEVYETAE DRDKNKPNDW
260 270 280 290 300
TVPKGCFMAN VADKSRFTNV KQFIAPETSE GVSLQLGNTK DFIISFDLKF
310 320 330 340 350
LTNGSVSVVL ETTEKNQLFT IHYVSNAQLI AFKERDIYYG IGPRTSWSTV
360 370 380 390 400
TRDLVTDLRK GVGLSNTKAV KPTKIMPKKV VRLIAKGKGF LDNITISTTA
410 420 430 440 450
HMAAFFAASD WLVRNQDEKG GWPIMVTRKL GEGFKSLEPG WYSAMAQGQA
460 470 480 490 500
ISTLVRAYLL TKDHIFLNSA LRATAPYKFL SEQHGVKAVF MNKHDWYEEY
510 520 530 540 550
PTTPSSFVLN GFMYSLIGLY DLKETAGEKL GKEARSLYER GMESLKAMLP
560 570 580 590 600
LYDTGSGTIY DLRHFMLGIA PNLARWDYHT THINQLQLLS TIDESPVFKE
610
FVKRWKSYLK GSRAKHN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0YNP1 | H0YNP1_HUMAN | Heparosan-N-sulfate-glucuronate 5-e... | GLCE | 553 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055837 | 65 | M → V. Corresponds to variant dbSNP:rs12440300Ensembl. | 1 | |
Natural variantiVAR_057958 | 597 | V → I1 PublicationCorresponds to variant dbSNP:rs3865014Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY635582 mRNA Translation: AAT48654.1 AC026992 mRNA No translation available. AB020643 mRNA Translation: BAA74859.1 |
CCDSi | CCDS32277.1 |
RefSeqi | NP_001311022.1, NM_001324093.1 NP_001311023.1, NM_001324094.1 NP_056369.1, NM_015554.2 XP_005254355.1, XM_005254298.3 |
Genome annotation databases
Ensembli | ENST00000261858; ENSP00000261858; ENSG00000138604 |
GeneIDi | 26035 |
KEGGi | hsa:26035 |
UCSCi | uc002ary.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY635582 mRNA Translation: AAT48654.1 AC026992 mRNA No translation available. AB020643 mRNA Translation: BAA74859.1 |
CCDSi | CCDS32277.1 |
RefSeqi | NP_001311022.1, NM_001324093.1 NP_001311023.1, NM_001324094.1 NP_056369.1, NM_015554.2 XP_005254355.1, XM_005254298.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6HZZ | X-ray | 2.52 | A/B | 98-617 | [»] | |
6I01 | X-ray | 2.10 | A/B | 98-617 | [»] | |
6I02 | X-ray | 2.45 | A/B | 98-617 | [»] | |
SMRi | O94923 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117501, 27 interactors |
IntActi | O94923, 20 interactors |
MINTi | O94923 |
STRINGi | 9606.ENSP00000261858 |
PTM databases
GlyGeni | O94923, 4 sites, 1 O-linked glycan (1 site) |
iPTMneti | O94923 |
PhosphoSitePlusi | O94923 |
SwissPalmi | O94923 |
Genetic variation databases
BioMutai | GLCE |
Proteomic databases
EPDi | O94923 |
jPOSTi | O94923 |
MassIVEi | O94923 |
MaxQBi | O94923 |
PaxDbi | O94923 |
PeptideAtlasi | O94923 |
PRIDEi | O94923 |
ProteomicsDBi | 50559 |
Protocols and materials databases
Antibodypediai | 26368, 64 antibodies |
DNASUi | 26035 |
Genome annotation databases
Ensembli | ENST00000261858; ENSP00000261858; ENSG00000138604 |
GeneIDi | 26035 |
KEGGi | hsa:26035 |
UCSCi | uc002ary.2, human |
Organism-specific databases
CTDi | 26035 |
DisGeNETi | 26035 |
GeneCardsi | GLCE |
HGNCi | HGNC:17855, GLCE |
HPAi | ENSG00000138604, Tissue enhanced (brain) |
MIMi | 612134, gene |
neXtProti | NX_O94923 |
OpenTargetsi | ENSG00000138604 |
PharmGKBi | PA145148750 |
VEuPathDBi | HostDB:ENSG00000138604.9 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3760, Eukaryota |
GeneTreei | ENSGT00390000006043 |
InParanoidi | O94923 |
OMAi | RGVFMYF |
OrthoDBi | 561819at2759 |
PhylomeDBi | O94923 |
TreeFami | TF105869 |
Enzyme and pathway databases
UniPathwayi | UPA00756 UPA00862 |
BRENDAi | 5.1.3.17, 2681 |
PathwayCommonsi | O94923 |
Reactomei | R-HSA-2022928, HS-GAG biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 26035, 10 hits in 961 CRISPR screens |
ChiTaRSi | GLCE, human |
GeneWikii | GLCE |
GenomeRNAii | 26035 |
Pharosi | O94923, Tbio |
PROi | PR:O94923 |
RNActi | O94923, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000138604, Expressed in testis and 206 other tissues |
ExpressionAtlasi | O94923, baseline and differential |
Genevisiblei | O94923, HS |
Family and domain databases
InterProi | View protein in InterPro IPR010598, C5-epim_C IPR039721, C5-epimerase |
PANTHERi | PTHR13174, PTHR13174, 1 hit |
Pfami | View protein in Pfam PF06662, C5-epim_C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLCE_HUMAN | |
Accessioni | O94923Primary (citable) accession number: O94923 Secondary accession number(s): Q6GUQ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 15, 2002 |
Last sequence update: | June 16, 2009 | |
Last modified: | April 7, 2021 | |
This is version 160 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families