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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2

Gene

GFPT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.By similarity

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (GFPT1), Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (GFPT2)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei427SubstrateBy similarity1
Binding sitei578SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-446210 Synthesis of UDP-N-acetyl-glucosamine
UniPathwayi
UPA00113;UER00528

Protein family/group databases

MEROPSiC44.972

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (EC:2.6.1.16By similarity)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 2
Glutamine:fructose-6-phosphate amidotransferase 2
Short name:
GFAT 2
Short name:
GFAT2
Hexosephosphate aminotransferase 2
Gene namesi
Name:GFPT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000131459.12
HGNCiHGNC:4242 GFPT2
MIMi603865 gene
neXtProtiNX_O94808

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi9945
OpenTargetsiENSG00000131459
PharmGKBiPA28652

Chemistry databases

DrugBankiDB00130 L-Glutamine

Polymorphism and mutation databases

BioMutaiGFPT2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001352832 – 682Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2Add BLAST681

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei244PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO94808
MaxQBiO94808
PaxDbiO94808
PeptideAtlasiO94808
PRIDEiO94808
ProteomicsDBi50448

PTM databases

iPTMnetiO94808
PhosphoSitePlusiO94808

Expressioni

Tissue specificityi

Highest levels of expression in heart, placenta, and spinal cord.

Gene expression databases

BgeeiENSG00000131459 Expressed in 194 organ(s), highest expression level in layer of synovial tissue
CleanExiHS_GFPT2
ExpressionAtlasiO94808 baseline and differential
GenevisibleiO94808 HS

Organism-specific databases

HPAiHPA056892
HPA059910

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
POT1Q9NUX52EBI-6916534,EBI-752420

Protein-protein interaction databases

BioGridi115270, 36 interactors
IntActiO94808, 2 interactors
STRINGi9606.ENSP00000253778

Structurei

3D structure databases

ProteinModelPortaliO94808
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 288Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST287
Domaini360 – 499SIS 1PROSITE-ProRule annotationAdd BLAST140
Domaini531 – 672SIS 2PROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni377 – 378Substrate-bindingBy similarity2
Regioni422 – 424Substrate-bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiKOG1268 Eukaryota
COG0449 LUCA
GeneTreeiENSGT00390000010049
HOGENOMiHOG000258898
HOVERGENiHBG051724
InParanoidiO94808
KOiK00820
OMAiRLDSSTC
OrthoDBiEOG091G02N5
PhylomeDBiO94808
TreeFamiTF300864

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR005855 GlmS_trans
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 2 hits
TIGRFAMsiTIGR01135 glmS, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

O94808-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK
60 70 80 90 100
ERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS
110 120 130 140 150
AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA
160 170 180 190 200
KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRR
210 220 230 240 250
GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHA
260 270 280 290 300
VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS
310 320 330 340 350
DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN
360 370 380 390 400
TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE
410 420 430 440 450
LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGVTNT
460 470 480 490 500
VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL
510 520 530 540 550
QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS LLVMGRGYNY
560 570 580 590 600
ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF
610 620 630 640 650
AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS
660 670 680
VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE
Length:682
Mass (Da):76,931
Last modified:January 23, 2007 - v3
Checksum:iA090D75526F3192B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RJP4E5RJP4_HUMAN
Glutamine--fructose-6-phosphate ami...
GFPT2
216Annotation score:
D6RAC1D6RAC1_HUMAN
Glutamine--fructose-6-phosphate ami...
GFPT2
178Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_013311471I → V1 PublicationCorresponds to variant dbSNP:rs2303007Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016789 mRNA Translation: BAA74731.1
BT009818 mRNA Translation: AAP88820.1
BC000012 mRNA Translation: AAH00012.1
CCDSiCCDS43411.1
RefSeqiNP_005101.1, NM_005110.3
UniGeneiHs.696497

Genome annotation databases

EnsembliENST00000253778; ENSP00000253778; ENSG00000131459
GeneIDi9945
KEGGihsa:9945
UCSCiuc003mlw.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGFPT2_HUMAN
AccessioniPrimary (citable) accession number: O94808
Secondary accession number(s): Q53XM2, Q9BWS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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