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UniProtKB - O94751 (BUB1_SCHPO)
Protein
Checkpoint serine/threonine-protein kinase bub1
Gene
bub1
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Involved in cell cycle checkpoint enforcement. Acts to stabilize the spindle during mitosis. Required for the correct localization of bub3 and mad3 to the kinetochore. Appears to have a role in chromosome segregation. Catalyzes the phosphorylation of bub3.
3 PublicationsCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 762 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 861 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 724 – 732 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- histone kinase activity (H2A-S121 specific) Source: PomBase
- identical protein binding Source: IntAct
- protein kinase activity Source: GO_Central
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
GO - Biological processi
- attachment of mitotic spindle microtubules to kinetochore Source: PomBase
- cell division Source: UniProtKB-KW
- homologous chromosome segregation Source: PomBase
- meiosis I spindle assembly checkpoint signaling Source: PomBase
- meiotic centromeric cohesion protection Source: PomBase
- meiotic sister chromatid cohesion, centromeric Source: GO_Central
- meiotic spindle assembly checkpoint signaling Source: PomBase
- mitotic sister chromatid biorientation Source: PomBase
- mitotic spindle assembly checkpoint signaling Source: PomBase
- regulation of homologous chromosome segregation Source: PomBase
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Chromosome partition, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 5613 |
Reactomei | R-SPO-141430, Inactivation of APC/C via direct inhibition of the APC/C complex |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:bub11 Publication ORF Names:SPCC1322.12cImported |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPCC1322.12c, bub1 |
VEuPathDBi | FungiDB:SPCC1322.12c |
Subcellular locationi
Nucleus
Other locations
Note: Associates with kinetochores and centromeres during the early stages of mitosis.
Cytoskeleton
- mitotic spindle pole body Source: PomBase
- old mitotic spindle pole body Source: PomBase
Nucleus
- nucleus Source: PomBase
Other locations
- bub1-bub3 complex Source: PomBase
- chromosome, centromeric core domain Source: PomBase
- chromosome, centromeric region Source: PomBase
- kinetochore Source: PomBase
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, NucleusPathology & Biotechi
Disruption phenotypei
Simultaneous disruption of nucleoporin alm1 results in a growth defect.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 78 | A → V: Defect in activating the spindle assembly checkpoint. No localization of bub1, bub3 or mad3 to the kinetochore. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085675 | 1 – 1044 | Checkpoint serine/threonine-protein kinase bub1Add BLAST | 1044 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 550 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Autophosphorylated.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | O94751 |
PaxDbi | O94751 |
PTM databases
iPTMneti | O94751 |
Interactioni
Subunit structurei
Interacts with bub3 and mad3.
1 PublicationBinary interactionsi
O94751
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-1002539,EBI-1002539 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 275320, 47 interactors |
DIPi | DIP-37961N |
IntActi | O94751, 6 interactors |
STRINGi | 4896.SPCC1322.12c.1 |
Structurei
3D structure databases
AlphaFoldDBi | O94751 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 204 | BUB1 N-terminalPROSITE-ProRule annotationAdd BLAST | 169 | |
Domaini | 718 – 1044 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 327 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 209 – 259 | DisorderedSequence analysisAdd BLAST | 51 | |
Regioni | 317 – 343 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 404 – 446 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 484 – 555 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 685 – 705 | DisorderedSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 209 – 258 | Polar residuesSequence analysisAdd BLAST | 50 | |
Compositional biasi | 404 – 444 | Polar residuesSequence analysisAdd BLAST | 41 | |
Compositional biasi | 498 – 555 | Polar residuesSequence analysisAdd BLAST | 58 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG1166, Eukaryota |
HOGENOMi | CLU_002115_1_0_1 |
InParanoidi | O94751 |
PhylomeDBi | O94751 |
Family and domain databases
InterProi | View protein in InterPro IPR015661, Bub1/Mad3 IPR011009, Kinase-like_dom_sf IPR013212, Mad3/Bub1_I IPR000719, Prot_kinase_dom |
PANTHERi | PTHR14030, PTHR14030, 1 hit |
Pfami | View protein in Pfam PF08311, Mad3_BUB1_I, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00777, Mad3_BUB1_I, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51489, BUB1_N, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
i Sequence
Sequence statusi: Complete.
O94751-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDWRLTENV LDQNIPETKP RESKTRLEEI QRLALFQEEL DIIEELDDPV
60 70 80 90 100
DVWYRCIEWL LETRFLGMET VNKMLDDAIQ YLERCRFALN DVRHLLIQLA
110 120 130 140 150
KIKQSYETPD ELQQAAKQFY QLASKGIGLE LALFYEEYGS LLIRMQRWKE
160 170 180 190 200
ASEVFHAAVS REARPLVRLL RNAAEFSRAY DLHNAHPSIH DAPYSSPFPP
210 220 230 240 250
PRIVLGSKPV SSSTLPSKPK SFQVFSDASS SRDSQNASDL PQAKSLESEA
260 270 280 290 300
NTPNLPLLYD KSSGKRVEYS AFNFLALYEN GEERSMEECR AQRYLSSIQP
310 320 330 340 350
NTAASFPKVV PKNEISVHHD SSSSNVSPIY KNPVAEQSDT PTRSLPKNYA
360 370 380 390 400
YVAKSTSPEL KVFDTVMPVA LSPKPAQKPP SPTIHTKAAL ADILDIFNQP
410 420 430 440 450
LRSESLEKSS KSPISAQSSY LGTPLKNDEN SSNSGATSLT GRSQEEHLDF
460 470 480 490 500
IPSLTPSKNY PSKIYSPNKN LDFSHTASKA ETYKNSNELE NVKREQPFSE
510 520 530 540 550
LLPSTLQEET ATGTTSTTFA NAKRRPEDSN ISPTNPKKLH TLPRSPQYST
560 570 580 590 600
VDSNSVLSPA MPKGYMFVNE NQSMKHESSV SNPVATIPHE NGKHDFGQLS
610 620 630 640 650
PIEHKPFFPK NDDELPGPSG YLTMPYEEAM ASLSNLPTLI NPLDQSLRDL
660 670 680 690 700
LFQVLRPSLL RDKDYHEHET SFALVEHIES FVSKIKPKAG GPGRRRSSNR
710 720 730 740 750
HSLDGPEFHL FYPPNTNLSV ISKLGQGAFA PVYLVKSKIE TENGDVSQGG
760 770 780 790 800
AENNESKLFA LKIETPPSCF EFYLTRQAMT RLKGLRETNS ILPVHQLHMF
810 820 830 840 850
HDTSHLLMDY RPQGSILDLV NSMHNSTFSS SGMDEILVVF FSIEFLRIIE
860 870 880 890 900
ALHTHKIIHG DLKADNALLR LETVADSEWS PIYSPEGLYG WSFKGIYLID
910 920 930 940 950
FGRGIDLSLF EEKVKFIADW DTDLQDCIEM REGRPWTYQI DYHGLAAIIY
960 970 980 990 1000
TMLFGQYIET RIEVINGQRR QVLTQRMKRY WNQDLWHRLF DLLLNPTLHV
1010 1020 1030 1040
SEENLPMTEE LSKIRIEMEE WLVNHSTGGS GLKGLLKSIE KRKI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF064796 Genomic DNA Translation: AAC98348.1 CU329672 Genomic DNA Translation: CAA22865.1 |
PIRi | T43800 |
RefSeqi | NP_588140.1, NM_001023130.2 |
Genome annotation databases
EnsemblFungii | SPCC1322.12c.1; SPCC1322.12c.1:pep; SPCC1322.12c |
GeneIDi | 2538736 |
KEGGi | spo:SPCC1322.12c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF064796 Genomic DNA Translation: AAC98348.1 CU329672 Genomic DNA Translation: CAA22865.1 |
PIRi | T43800 |
RefSeqi | NP_588140.1, NM_001023130.2 |
3D structure databases
AlphaFoldDBi | O94751 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 275320, 47 interactors |
DIPi | DIP-37961N |
IntActi | O94751, 6 interactors |
STRINGi | 4896.SPCC1322.12c.1 |
PTM databases
iPTMneti | O94751 |
Proteomic databases
MaxQBi | O94751 |
PaxDbi | O94751 |
Genome annotation databases
EnsemblFungii | SPCC1322.12c.1; SPCC1322.12c.1:pep; SPCC1322.12c |
GeneIDi | 2538736 |
KEGGi | spo:SPCC1322.12c |
Organism-specific databases
PomBasei | SPCC1322.12c, bub1 |
VEuPathDBi | FungiDB:SPCC1322.12c |
Phylogenomic databases
eggNOGi | KOG1166, Eukaryota |
HOGENOMi | CLU_002115_1_0_1 |
InParanoidi | O94751 |
PhylomeDBi | O94751 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 5613 |
Reactomei | R-SPO-141430, Inactivation of APC/C via direct inhibition of the APC/C complex |
Miscellaneous databases
PROi | PR:O94751 |
Family and domain databases
InterProi | View protein in InterPro IPR015661, Bub1/Mad3 IPR011009, Kinase-like_dom_sf IPR013212, Mad3/Bub1_I IPR000719, Prot_kinase_dom |
PANTHERi | PTHR14030, PTHR14030, 1 hit |
Pfami | View protein in Pfam PF08311, Mad3_BUB1_I, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00777, Mad3_BUB1_I, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51489, BUB1_N, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BUB1_SCHPO | |
Accessioni | O94751Primary (citable) accession number: O94751 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - SIMILARITY comments
Index of protein domains and families