UniProtKB - O94742 (SEM1_YEAST)
Protein
26S proteasome complex subunit SEM1
Gene
SEM1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Versatile protein that might stabilize multiple protein complexes involved in diverse pathways. Subunit of the 26S proteasome which plays a role in ubiquitin-dependent proteolysis. Associates also with the TREX-2 complex that is required for transcription-coupled mRNA export, and the COP9 signalosome, which is involved in deneddylation.2 Publications
Miscellaneous
Present with 5590 molecules/cell in log phase SD medium.1 Publication
GO - Biological processi
- double-strand break repair via homologous recombination Source: GO_Central
- filamentous growth Source: SGD
- histone deubiquitination Source: SGD
- maintenance of DNA trinucleotide repeats Source: SGD
- mRNA export from nucleus Source: SGD
- proteasome assembly Source: SGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
- regulation of cell cycle Source: SGD
- SAGA complex localization to transcription regulatory region Source: SGD
- ubiquitin-dependent protein catabolic process Source: SGD
Keywordsi
Biological process | Transcription |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome complex subunit SEM1 |
Gene namesi | Name:SEM1 Synonyms:DSH1 Ordered Locus Names:YDR363W-A |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000007235, SEM1 |
VEuPathDBi | FungiDB:YDR363W-A |
Subcellular locationi
Cytosol
- cytosol Source: SGD
- proteasome storage granule Source: SGD
Nucleus
- transcription export complex 2 Source: SGD
Other locations
- proteasome complex Source: GO_Central
- proteasome regulatory particle, lid subcomplex Source: SGD
Keywords - Cellular componenti
ProteasomePathology & Biotechi
Disruption phenotypei
Impairs mRNA export and transcription elongation, and induces strong transcription-associated hyperrecombination phenotypes.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000122970 | 2 – 89 | 26S proteasome complex subunit SEM1Add BLAST | 88 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 12 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | O94742 |
PaxDbi | O94742 |
PRIDEi | O94742 |
PTM databases
iPTMneti | O94742 |
Interactioni
Subunit structurei
Part of the 26S proteasome. Associates with the nuclear pore complex (NPC)-associated TREX-2 complex and the COP9 signalosome.
Interacts with CSN12 and THP3.
2 PublicationsBinary interactionsi
Hide detailsO94742
With | #Exp. | IntAct |
---|---|---|
HSM3 [P38348] | 2 | EBI-31337,EBI-21152 |
RPN7 [Q06103] | 3 | EBI-31337,EBI-15940 |
THP1 [Q08231] | 2 | EBI-31337,EBI-32097 |
Protein-protein interaction databases
BioGRIDi | 32420, 497 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-8754N |
IntActi | O94742, 18 interactors |
MINTi | O94742 |
STRINGi | 4932.YDR363W-A |
Miscellaneous databases
RNActi | O94742, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O94742 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the DSS1/SEM1 family.Curated
Phylogenomic databases
eggNOGi | KOG4764, Eukaryota |
HOGENOMi | CLU_141774_1_0_1 |
InParanoidi | O94742 |
OMAi | IWEENWD |
Family and domain databases
DisProti | DP01014 |
InterProi | View protein in InterPro IPR007834, DSS1_SEM1 |
PANTHERi | PTHR16771, PTHR16771, 1 hit |
Pfami | View protein in Pfam PF05160, DSS1_SEM1, 1 hit |
SMARTi | View protein in SMART SM01385, DSS1_SEM1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O94742-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTDVAAAQA QSKIDLTKKK NEEINKKSLE EDDEFEDFPI DTWANGETIK
60 70 80
SNAVTQTNIW EENWDDVEVD DDFTNELKAE LDRYKRENQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF059310 mRNA Translation: AAD08804.1 AF065136 Genomic DNA Translation: AAC96096.1 U28372 Genomic DNA No translation available. AY557719 Genomic DNA Translation: AAS56045.1 BK006938 Genomic DNA Translation: DAA12202.1 |
PIRi | S78744 |
RefSeqi | NP_010651.3, NM_001184328.3 |
Genome annotation databases
EnsemblFungii | YDR363W-A_mRNA; YDR363W-A; YDR363W-A |
GeneIDi | 851967 |
KEGGi | sce:YDR363W-A |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF059310 mRNA Translation: AAD08804.1 AF065136 Genomic DNA Translation: AAC96096.1 U28372 Genomic DNA No translation available. AY557719 Genomic DNA Translation: AAS56045.1 BK006938 Genomic DNA Translation: DAA12202.1 |
PIRi | S78744 |
RefSeqi | NP_010651.3, NM_001184328.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCK | electron microscopy | 3.50 | I | 1-89 | [»] | |
3JCO | electron microscopy | 4.80 | Y | 1-89 | [»] | |
3JCP | electron microscopy | 4.60 | Y | 1-89 | [»] | |
3T5V | X-ray | 2.90 | C/F | 1-89 | [»] | |
4CR2 | electron microscopy | 7.70 | Y | 1-89 | [»] | |
4CR3 | electron microscopy | 9.30 | Y | 1-89 | [»] | |
4CR4 | electron microscopy | 8.80 | Y | 1-89 | [»] | |
4TRQ | X-ray | 3.10 | C/F | 30-89 | [»] | |
5A5B | electron microscopy | 9.50 | Y | 1-89 | [»] | |
5G5P | electron microscopy | 5.30 | C | 1-89 | [»] | |
5L3T | X-ray | 4.93 | C | 1-89 | [»] | |
5MPB | electron microscopy | 7.80 | Y | 1-89 | [»] | |
5MPC | electron microscopy | 7.70 | Y | 1-89 | [»] | |
5MPD | electron microscopy | 4.10 | Y | 1-89 | [»] | |
5MPE | electron microscopy | 4.50 | Y | 1-89 | [»] | |
5UBP | X-ray | 2.30 | C | 1-89 | [»] | |
5WVI | electron microscopy | 6.30 | Y | 1-89 | [»] | |
5WVK | electron microscopy | 4.20 | Y | 1-89 | [»] | |
6FVT | electron microscopy | 4.10 | Y | 1-89 | [»] | |
6FVU | electron microscopy | 4.50 | Y | 1-89 | [»] | |
6FVV | electron microscopy | 5.40 | Y | 1-89 | [»] | |
6FVW | electron microscopy | 4.50 | Y | 1-89 | [»] | |
6FVX | electron microscopy | 4.90 | Y | 1-89 | [»] | |
6FVY | electron microscopy | 6.10 | Y | 1-89 | [»] | |
6J2C | electron microscopy | 7.00 | Y | 1-89 | [»] | |
6J2N | electron microscopy | 7.50 | Y | 1-89 | [»] | |
6J2Q | electron microscopy | 3.80 | Y | 1-89 | [»] | |
6J2X | electron microscopy | 3.80 | Y | 1-89 | [»] | |
6J30 | electron microscopy | 4.50 | Y | 1-89 | [»] | |
SMRi | O94742 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32420, 497 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-8754N |
IntActi | O94742, 18 interactors |
MINTi | O94742 |
STRINGi | 4932.YDR363W-A |
PTM databases
iPTMneti | O94742 |
Proteomic databases
MaxQBi | O94742 |
PaxDbi | O94742 |
PRIDEi | O94742 |
Genome annotation databases
EnsemblFungii | YDR363W-A_mRNA; YDR363W-A; YDR363W-A |
GeneIDi | 851967 |
KEGGi | sce:YDR363W-A |
Organism-specific databases
SGDi | S000007235, SEM1 |
VEuPathDBi | FungiDB:YDR363W-A |
Phylogenomic databases
eggNOGi | KOG4764, Eukaryota |
HOGENOMi | CLU_141774_1_0_1 |
InParanoidi | O94742 |
OMAi | IWEENWD |
Miscellaneous databases
PROi | PR:O94742 |
RNActi | O94742, protein |
Family and domain databases
DisProti | DP01014 |
InterProi | View protein in InterPro IPR007834, DSS1_SEM1 |
PANTHERi | PTHR16771, PTHR16771, 1 hit |
Pfami | View protein in Pfam PF05160, DSS1_SEM1, 1 hit |
SMARTi | View protein in SMART SM01385, DSS1_SEM1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SEM1_YEAST | |
Accessioni | O94742Primary (citable) accession number: O94742 Secondary accession number(s): D6VSZ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families