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UniProtKB - O94347 (NNRD_SCHPO)
Protein
ATP-dependent (S)-NAD(P)H-hydrate dehydratase
Gene
SPCC61.03
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
UniRule annotationCatalytic activityi
Cofactori
Mg2+UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 121 | NADPHX; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 238 | NADPHXUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 209 – 213 | ATPUniRule annotation | 5 | |
Nucleotide bindingi | 228 – 237 | ATPUniRule annotation | 10 |
GO - Molecular functioni
- ADP-dependent NAD(P)H-hydrate dehydratase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- ATP-dependent NAD(P)H-hydrate dehydratase activity Source: GO_Central
GO - Biological processi
- metabolite repair Source: GO_Central
- nicotinamide nucleotide metabolic process Source: PomBase
Keywordsi
Molecular function | Lyase |
Ligand | ATP-binding, NAD, NADP, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SPO-197264, Nicotinamide salvaging |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.93UniRule annotation)Alternative name(s): ATP-dependent NAD(P)HX dehydrataseUniRule annotation |
Gene namesi | ORF Names:SPCC61.03 |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPCC61.03 |
VEuPathDBi | FungiDB:SPCC61.03 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Cytosol
- cytosol Source: PomBase
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000315959 | 1 – 327 | ATP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST | 327 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | O94347 |
PaxDbi | O94347 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 276063, 15 interactors |
STRINGi | 4896.SPCC61.03.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 313 | YjeF C-terminalUniRule annotationAdd BLAST | 303 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 174 – 180 | NADPHXUniRule annotation | 7 |
Sequence similaritiesi
Belongs to the NnrD/CARKD family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3974, Eukaryota |
HOGENOMi | CLU_030651_3_0_1 |
InParanoidi | O94347 |
OMAi | HIPHTNE |
PhylomeDBi | O94347 |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit |
PROSITEi | View protein in PROSITE PS01049, YJEF_C_1, 1 hit PS51383, YJEF_C_3, 1 hit |
i Sequence
Sequence statusi: Complete.
O94347-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSGSPKITN LLTRVKRIIP PLLDTFHKGQ AGRVGVFGGC QHYTGAPYYS
60 70 80 90 100
SMSSMLFGSD QSHIFCEKEA ANVIKSYSPD LIVHPFLREK DKAGPEDSVD
110 120 130 140 150
KCFELIKPMM GRLHAIVIGP GLGRDEWMQE IMAKVIEYAR KNDMPMVIDA
160 170 180 190 200
DGLWLIQQRP ELVSGYHNVI LTPNVIEFKR LCDKLDIKSD GPDACNQLAG
210 220 230 240 250
KLNLLIIQKG QSDIISDGAT AYACSVPGGL KRCGGQGDIL TGILATFLAW
260 270 280 290 300
RHAYLSKEWD TEGNMDAKEC LFLAAFGASA CTRWCSRLAF KECGRATQST
310 320
DLVRHVGKAY NALMEDEIPS VEEKIKD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329672 Genomic DNA Translation: CAA22272.1 |
PIRi | T41463 |
RefSeqi | NP_588194.1, NM_001023184.2 |
Genome annotation databases
EnsemblFungii | SPCC61.03.1; SPCC61.03.1:pep; SPCC61.03 |
GeneIDi | 2539500 |
KEGGi | spo:SPCC61.03 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329672 Genomic DNA Translation: CAA22272.1 |
PIRi | T41463 |
RefSeqi | NP_588194.1, NM_001023184.2 |
3D structure databases
AlphaFoldDBi | O94347 |
SMRi | O94347 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 276063, 15 interactors |
STRINGi | 4896.SPCC61.03.1 |
Proteomic databases
MaxQBi | O94347 |
PaxDbi | O94347 |
Genome annotation databases
EnsemblFungii | SPCC61.03.1; SPCC61.03.1:pep; SPCC61.03 |
GeneIDi | 2539500 |
KEGGi | spo:SPCC61.03 |
Organism-specific databases
PomBasei | SPCC61.03 |
VEuPathDBi | FungiDB:SPCC61.03 |
Phylogenomic databases
eggNOGi | KOG3974, Eukaryota |
HOGENOMi | CLU_030651_3_0_1 |
InParanoidi | O94347 |
OMAi | HIPHTNE |
PhylomeDBi | O94347 |
Enzyme and pathway databases
Reactomei | R-SPO-197264, Nicotinamide salvaging |
Miscellaneous databases
PROi | PR:O94347 |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit |
PROSITEi | View protein in PROSITE PS01049, YJEF_C_1, 1 hit PS51383, YJEF_C_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NNRD_SCHPO | |
Accessioni | O94347Primary (citable) accession number: O94347 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - SIMILARITY comments
Index of protein domains and families