SPCC61.03

Functionⁱ

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.UniRule annotation

Catalytic activityⁱ

ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH.UniRule annotation

ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH.

Cofactorⁱ

Mg²⁺UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	121	NAD(P)HX; via amide nitrogenUniRule annotation		1
Binding siteⁱ	238	NAD(P)HXUniRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	209 – 213	ATPUniRule annotation		5
Nucleotide bindingⁱ	228 – 237	ATPUniRule annotation		10

GO - Molecular functionⁱ

ADP-dependent NAD(P)H-hydrate dehydratase activity Source: InterPro
ATP binding Source: UniProtKB-KW
ATP-dependent NAD(P)H-hydrate dehydratase activity Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

nicotinamide nucleotide metabolic process Source: PomBase

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Ligand	ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-SPO-197264 Nicotinamide salvaging

Reactomeⁱ

R-SPO-197264 Nicotinamide salvaging

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ATP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.93UniRule annotation) Alternative name(s): ATP-dependent NAD(P)HX dehydrataseUniRule annotation
Gene namesⁱ	ORF Names:SPCC61.03
Organismⁱ	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifierⁱ	284812 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe
Proteomesⁱ	UP000002485 Componentⁱ: Chromosome III

Organism-specific databases

EuPathDBⁱ	FungiDB:SPCC61.03
PomBaseⁱ	SPCC61.03

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000315959	1 – 327	ATP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST		327

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O94347
PaxDbⁱ	O94347
PRIDEⁱ	O94347

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	276063, 14 interactors
STRINGⁱ	4896.SPCC61.03.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O94347
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	11 – 313	YjeF C-terminalUniRule annotationAdd BLAST		303

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	174 – 180	NAD(P)HXUniRule annotation		7

Sequence similaritiesⁱ

Belongs to the NnrD/CARKD family.UniRule annotation

Phylogenomic databases

HOGENOMⁱ	HOG000163126
InParanoidⁱ	O94347
KEGG Orthology (KO) More...KOⁱ	K17757
OMAⁱ	NLMVHPL
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4N4W
PhylomeDBⁱ	O94347

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01171 YXKO-related, 1 hit
Gene3Dⁱ	3.40.1190.20, 1 hit
HAMAPⁱ	MF_01965 NADHX_dehydratase, 1 hit
InterProⁱ	View protein in InterPro IPR017953 Carbohydrate_kinase_pred_CS IPR000631 CARKD IPR029056 Ribokinase-like
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01256 Carb_kinase, 1 hit
SUPFAMⁱ	SSF53613 SSF53613, 1 hit
TIGRFAMsⁱ	TIGR00196 yjeF_cterm, 1 hit
PROSITEⁱ	View protein in PROSITE PS01049 YJEF_C_1, 1 hit PS51383 YJEF_C_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O94347-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTSGSPKITN LLTRVKRIIP PLLDTFHKGQ AGRVGVFGGC QHYTGAPYYS 
        60         70         80         90        100
SMSSMLFGSD QSHIFCEKEA ANVIKSYSPD LIVHPFLREK DKAGPEDSVD 
       110        120        130        140        150
KCFELIKPMM GRLHAIVIGP GLGRDEWMQE IMAKVIEYAR KNDMPMVIDA 
       160        170        180        190        200
DGLWLIQQRP ELVSGYHNVI LTPNVIEFKR LCDKLDIKSD GPDACNQLAG 
       210        220        230        240        250
KLNLLIIQKG QSDIISDGAT AYACSVPGGL KRCGGQGDIL TGILATFLAW 
       260        270        280        290        300
RHAYLSKEWD TEGNMDAKEC LFLAAFGASA CTRWCSRLAF KECGRATQST 
       310        320 
DLVRHVGKAY NALMEDEIPS VEEKIKD

Length:327

Mass (Da):36,094

Last modified:May 1, 1999 - v1

Checksum:ⁱ5DB8746FD47D75A8

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CU329672 Genomic DNA Translation: CAA22272.1
PIRⁱ	T41463
NCBI Reference Sequences More...RefSeqⁱ	NP_588194.1, NM_001023184.2

Genome annotation databases

EnsemblFungiⁱ	SPCC61.03.1; SPCC61.03.1:pep; SPCC61.03
GeneIDⁱ	2539500
KEGGⁱ	spo:SPCC61.03

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O94347	ATP-dependent (S)-NAD(P)H-hydrate dehydratase		SCHJY		323	UniRef50_O94347

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CU329672 Genomic DNA Translation: CAA22272.1
PIRⁱ	T41463
RefSeqⁱ	NP_588194.1, NM_001023184.2

3D structure databases

ProteinModelPortalⁱ	O94347
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	276063, 14 interactors
STRINGⁱ	4896.SPCC61.03.1

Proteomic databases

MaxQBⁱ	O94347
PaxDbⁱ	O94347
PRIDEⁱ	O94347

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	SPCC61.03.1; SPCC61.03.1:pep; SPCC61.03
GeneIDⁱ	2539500
KEGGⁱ	spo:SPCC61.03

Organism-specific databases

EuPathDBⁱ	FungiDB:SPCC61.03
PomBaseⁱ	SPCC61.03

Phylogenomic databases

HOGENOMⁱ	HOG000163126
InParanoidⁱ	O94347
KOⁱ	K17757
OMAⁱ	NLMVHPL
OrthoDBⁱ	EOG092C4N4W
PhylomeDBⁱ	O94347

Enzyme and pathway databases

Reactomeⁱ	R-SPO-197264 Nicotinamide salvaging

Reactomeⁱ

R-SPO-197264 Nicotinamide salvaging

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O94347

PROⁱ

PR:O94347

Family and domain databases

CDDⁱ	cd01171 YXKO-related, 1 hit
Gene3Dⁱ	3.40.1190.20, 1 hit
HAMAPⁱ	MF_01965 NADHX_dehydratase, 1 hit
InterProⁱ	View protein in InterPro IPR017953 Carbohydrate_kinase_pred_CS IPR000631 CARKD IPR029056 Ribokinase-like
Pfamⁱ	View protein in Pfam PF01256 Carb_kinase, 1 hit
SUPFAMⁱ	SSF53613 SSF53613, 1 hit
TIGRFAMsⁱ	TIGR00196 yjeF_cterm, 1 hit
PROSITEⁱ	View protein in PROSITE PS01049 YJEF_C_1, 1 hit PS51383 YJEF_C_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NNRD_SCHPO
Accessionⁱ	O94347Primary (citable) accession number: O94347
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
	Last sequence update:	May 1, 1999
	Last modified:	October 10, 2018
	This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

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Supporting data

UniProtKB - O94347 (NNRD_SCHPO)

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ATP-dependent (S)-NAD(P)H-hydrate dehydratase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Keywords - PTMi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ