Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent (S)-NAD(P)H-hydrate dehydratase

Gene

SPCC61.03

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.UniRule annotation

Catalytic activityi

ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH.UniRule annotation
ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH.

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei121NAD(P)HX; via amide nitrogenUniRule annotation1
Binding sitei238NAD(P)HXUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi209 – 213ATPUniRule annotation5
Nucleotide bindingi228 – 237ATPUniRule annotation10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-197264 Nicotinamide salvaging

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.93UniRule annotation)
Alternative name(s):
ATP-dependent NAD(P)HX dehydrataseUniRule annotation
Gene namesi
ORF Names:SPCC61.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC61.03
PomBaseiSPCC61.03

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003159591 – 327ATP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST327

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO94347
PaxDbiO94347
PRIDEiO94347

Interactioni

Protein-protein interaction databases

BioGridi276063, 14 interactors
STRINGi4896.SPCC61.03.1

Structurei

3D structure databases

ProteinModelPortaliO94347
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 313YjeF C-terminalUniRule annotationAdd BLAST303

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 180NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrD/CARKD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000163126
InParanoidiO94347
KOiK17757
OMAiNLMVHPL
OrthoDBiEOG092C4N4W
PhylomeDBiO94347

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
PROSITEiView protein in PROSITE
PS01049 YJEF_C_1, 1 hit
PS51383 YJEF_C_3, 1 hit

Sequencei

Sequence statusi: Complete.

O94347-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSGSPKITN LLTRVKRIIP PLLDTFHKGQ AGRVGVFGGC QHYTGAPYYS
60 70 80 90 100
SMSSMLFGSD QSHIFCEKEA ANVIKSYSPD LIVHPFLREK DKAGPEDSVD
110 120 130 140 150
KCFELIKPMM GRLHAIVIGP GLGRDEWMQE IMAKVIEYAR KNDMPMVIDA
160 170 180 190 200
DGLWLIQQRP ELVSGYHNVI LTPNVIEFKR LCDKLDIKSD GPDACNQLAG
210 220 230 240 250
KLNLLIIQKG QSDIISDGAT AYACSVPGGL KRCGGQGDIL TGILATFLAW
260 270 280 290 300
RHAYLSKEWD TEGNMDAKEC LFLAAFGASA CTRWCSRLAF KECGRATQST
310 320
DLVRHVGKAY NALMEDEIPS VEEKIKD
Length:327
Mass (Da):36,094
Last modified:May 1, 1999 - v1
Checksum:i5DB8746FD47D75A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA Translation: CAA22272.1
PIRiT41463
RefSeqiNP_588194.1, NM_001023184.2

Genome annotation databases

EnsemblFungiiSPCC61.03.1; SPCC61.03.1:pep; SPCC61.03
GeneIDi2539500
KEGGispo:SPCC61.03

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA Translation: CAA22272.1
PIRiT41463
RefSeqiNP_588194.1, NM_001023184.2

3D structure databases

ProteinModelPortaliO94347
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276063, 14 interactors
STRINGi4896.SPCC61.03.1

Proteomic databases

MaxQBiO94347
PaxDbiO94347
PRIDEiO94347

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC61.03.1; SPCC61.03.1:pep; SPCC61.03
GeneIDi2539500
KEGGispo:SPCC61.03

Organism-specific databases

EuPathDBiFungiDB:SPCC61.03
PomBaseiSPCC61.03

Phylogenomic databases

HOGENOMiHOG000163126
InParanoidiO94347
KOiK17757
OMAiNLMVHPL
OrthoDBiEOG092C4N4W
PhylomeDBiO94347

Enzyme and pathway databases

ReactomeiR-SPO-197264 Nicotinamide salvaging

Miscellaneous databases

PROiPR:O94347

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
PROSITEiView protein in PROSITE
PS01049 YJEF_C_1, 1 hit
PS51383 YJEF_C_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNNRD_SCHPO
AccessioniPrimary (citable) accession number: O94347
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: October 10, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again