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Protein

Xyloglucan-specific endo-beta-1,4-glucanase A

Gene

xgeA

Organism
Aspergillus aculeatus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan with retention of the beta-configuration of the glycosyl residues. Specific for xyloglucan and does not hydrolyze other cell wall components.2 Publications

Catalytic activityi

Xyloglucan + H2O = xyloglucan oligosaccharides.

pH dependencei

Optimum pH is 3.4. Stability declines sharply below pH 2.8 and above pH 5.0.1 Publication

Temperature dependencei

Optimum temperature is below 30 degrees Celsius. Is very stable below 35 degrees Celsius, but at 50 degrees Celsius, it loses 80 percent of its activity within 2 h.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16602
BRENDAi3.2.1.151 488

Protein family/group databases

CAZyiGH12 Glycoside Hydrolase Family 12
mycoCLAPiXEG12A_ASPAC

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan-specific endo-beta-1,4-glucanase A (EC:3.2.1.151)
Alternative name(s):
Xyloglucanase A
Xyloglucanendohydrolase A
Gene namesi
Name:xgeA
OrganismiAspergillus aculeatus
Taxonomic identifieri5053 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 14Sequence analysisAdd BLAST14
ChainiPRO_500005406215 – 238Xyloglucan-specific endo-beta-1,4-glucanase AAdd BLAST224

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO94218
SMRiO94218
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK18576

Family and domain databases

Gene3Di2.60.120.180, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR013319 GH11/12
IPR002594 GH12
PANTHERiPTHR34002 PTHR34002, 1 hit
PfamiView protein in Pfam
PF01670 Glyco_hydro_12, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O94218-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLSLLSLAT LASAASLQRR SDFCGQWDTA TAGDFTLYND LWGESAGTGS
60 70 80 90 100
QCTGVDSYSG DTIAWHTSWS WSGGSSSVKS YVNAALTFTP TQLNCISSIP
110 120 130 140 150
TTWKWSYSGS SIVADVAYDT FLAETASGSS KYEIMVWLAA LGGAGPISST
160 170 180 190 200
GSTIATPTIA GVNWKLYSGP NGDTTVYSFV ADSTTESFSG DLNDFFTYLV
210 220 230
DNEGVSDELY LTTLEAGTEP FTGSNAKLTV SEYSISIE
Length:238
Mass (Da):25,159
Last modified:May 1, 1999 - v1
Checksum:iFCCA6746D9AEC1B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043595 mRNA Translation: AAD02275.1
AY160774 mRNA Translation: AAO20340.1

Genome annotation databases

KEGGiag:AAD02275

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043595 mRNA Translation: AAD02275.1
AY160774 mRNA Translation: AAO20340.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VL8X-ray1.90A15-238[»]
3VL9X-ray1.20A/B15-238[»]
3VLBX-ray2.70B/D21-238[»]
ProteinModelPortaliO94218
SMRiO94218
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH12 Glycoside Hydrolase Family 12
mycoCLAPiXEG12A_ASPAC

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAD02275

Phylogenomic databases

KOiK18576

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16602
BRENDAi3.2.1.151 488

Family and domain databases

Gene3Di2.60.120.180, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR013319 GH11/12
IPR002594 GH12
PANTHERiPTHR34002 PTHR34002, 1 hit
PfamiView protein in Pfam
PF01670 Glyco_hydro_12, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiXGEA_ASPAC
AccessioniPrimary (citable) accession number: O94218
Secondary accession number(s): Q6YBY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 1, 1999
Last modified: December 20, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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