UniProtKB - O93610 (DPOD2_XENLA)
DNA polymerase delta subunit 2
pold2
Functioni
As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS as a component of the POLZ complex. Along with POLD3, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7.
By similarityGO - Molecular functioni
- DNA binding Source: InterPro
GO - Biological processi
- DNA repair Source: UniProtKB-KW
- DNA replication Source: UniProtKB-KW
Keywordsi
Biological process | DNA damage, DNA excision, DNA repair, DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase delta subunit 2Alternative name(s): XlCdc1 |
Gene namesi | Name:pold2 Synonyms:cdc1 |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-999393, pold2.L |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Recruited to DNA damage sites within 2 hours following UV irradiation.By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096168 | 1 – 463 | DNA polymerase delta subunit 2Add BLAST | 463 |
Proteomic databases
MaxQBi | O93610 |
Interactioni
Subunit structurei
Component of the tetrameric DNA polymerase delta complex (Pol-delta4), which consists of POLD1/p125, POLD2/p50, POLD3/p66/p68 and POLD4/p12, with POLD1 bearing DNA polymerase and 3' to 5' proofreading exonuclease activities. Following stress caused by DNA damaging agents or by replication stress, POLD4 is degraded and Pol-delta4 is converted into a trimeric form of the complex (Pol-delta3), which consists of POLD1, POLD2 and POLD3. Pol-delta3 is the major form occurring at S phase replication sites, as well as DNA damage sites. Also observed as a dimeric complex with POLD2 (Pol-delta2 complex).
Component of the DNA polymerase zeta complex (POLZ), which consists of REV3L, MAD2L2, POLD2 and POLD3, with REV3L bearing DNA polymerase catalytic activity.
By similarityProtein-protein interaction databases
BioGRIDi | 98035, 1 interactor |
IntActi | O93610, 2 interactors |
Family & Domainsi
Sequence similaritiesi
Family and domain databases
CDDi | cd07387, MPP_PolD2_C, 1 hit |
InterProi | View protein in InterPro IPR007185, DNA_pol_a/d/e_bsu IPR040663, DNA_pol_D_N IPR024826, DNA_pol_delta/II_ssu IPR041863, PolD2_C |
PANTHERi | PTHR10416, PTHR10416, 1 hit |
Pfami | View protein in Pfam PF18018, DNA_pol_D_N, 1 hit PF04042, DNA_pol_E_B, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MFTDLAISGG PGLLTAPSEV QSTFTRVSNT QYSNCSSIFR LGERTFTRQY
60 70 80 90 100
AHIYATRLEQ MRPLLIKSAK QRWGDDIAVR KLCELQGGEK CCVIGTLFKS
110 120 130 140 150
MELQPSILRE ISEEHNLLPQ PARQKYISDS DELILEDELQ RIKLEGATDV
160 170 180 190 200
QQLVTGAVLA VLGAEEDAGK FVVEDFCLTS LPVQSPLPRL SEDRFVLLTS
210 220 230 240 250
GLGLGGGSGD SLMGLQLLLD LVTGQAGAEE DQGCAARISR VILAGNLLSE
260 270 280 290 300
NTQGKDSLNK AKYLSKKTQA ASVEAVKMLD EILLQMSGSV SVDVMPGAFD
310 320 330 340 350
PTNYILPQQP LHRCMFPQSA LYSTLQLVTN PYEAEIDGVR FLGTSGQNIG
360 370 380 390 400
DIYKYSSMQD YLDILEWTLQ VGHLCPTAPD TLGCYPFYKS DPFILQNCPH
410 420 430 440 450
VYFCGSAPKF SCKEVTGAEG QRVLLLTVPE FCSTQTACLV NLRTLQCQPI
460
SFSGFGADDE LGD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ010836 mRNA Translation: CAA09373.1 BC047262 mRNA Translation: AAH47262.1 |
RefSeqi | NP_001080101.1, NM_001086632.1 |
Genome annotation databases
GeneIDi | 379793 |
KEGGi | xla:379793 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ010836 mRNA Translation: CAA09373.1 BC047262 mRNA Translation: AAH47262.1 |
RefSeqi | NP_001080101.1, NM_001086632.1 |
3D structure databases
AlphaFoldDBi | O93610 |
SMRi | O93610 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 98035, 1 interactor |
IntActi | O93610, 2 interactors |
Proteomic databases
MaxQBi | O93610 |
Protocols and materials databases
DNASUi | 379793 |
Genome annotation databases
GeneIDi | 379793 |
KEGGi | xla:379793 |
Organism-specific databases
CTDi | 379793 |
Xenbasei | XB-GENE-999393, pold2.L |
Family and domain databases
CDDi | cd07387, MPP_PolD2_C, 1 hit |
InterProi | View protein in InterPro IPR007185, DNA_pol_a/d/e_bsu IPR040663, DNA_pol_D_N IPR024826, DNA_pol_delta/II_ssu IPR041863, PolD2_C |
PANTHERi | PTHR10416, PTHR10416, 1 hit |
Pfami | View protein in Pfam PF18018, DNA_pol_D_N, 1 hit PF04042, DNA_pol_E_B, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOD2_XENLA | |
Accessioni | O93610Primary (citable) accession number: O93610 Secondary accession number(s): Q5D0A2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families