UniProtKB - O93541 (LDHA_CHAGU)
Protein
L-lactate dehydrogenase A chain
Gene
ldha
Organism
Champsocephalus gunnari (Mackerel icefish)
Status
Functioni
Catalytic activityi
- EC:1.1.1.27
: pyruvate fermentation to lactate Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.Proteins known to be involved in this subpathway in this organism are:
- L-lactate dehydrogenase A chain (ldha)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 98 | NADBy similarity | 1 | |
Binding sitei | 105 | SubstrateBy similarity | 1 | |
Binding sitei | 137 | NADBy similarity | 1 | |
Binding sitei | 137 | SubstrateBy similarity | 1 | |
Binding sitei | 168 | SubstrateBy similarity | 1 | |
Active sitei | 192 | Proton acceptorBy similarity | 1 | |
Binding sitei | 247 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 29 – 57 | NADBy similarityAdd BLAST | 29 |
GO - Molecular functioni
- L-lactate dehydrogenase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- carboxylic acid metabolic process Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.1.1.27, 9919 |
UniPathwayi | UPA00554;UER00611 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ldha |
Organismi | Champsocephalus gunnari (Mackerel icefish) |
Taxonomic identifieri | 52237 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Neoteleostei › Acanthomorphata › Eupercaria › Perciformes › Notothenioidei › Channichthyidae › Champsocephalus |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000168431 | 2 – 331 | L-lactate dehydrogenase A chainAdd BLAST | 330 |
Interactioni
Subunit structurei
Homotetramer.
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O93541 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O93541 |
Family & Domainsi
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
HAMAPi | MF_00488, Lactate_dehydrog, 1 hit |
InterProi | View protein in InterPro IPR001557, L-lactate/malate_DH IPR011304, L-lactate_DH IPR018177, L-lactate_DH_AS IPR022383, Lactate/malate_DH_C IPR001236, Lactate/malate_DH_N IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF02866, Ldh_1_C, 1 hit PF00056, Ldh_1_N, 1 hit |
PIRSFi | PIRSF000102, Lac_mal_DH, 1 hit |
PRINTSi | PR00086, LLDHDRGNASE |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
TIGRFAMsi | TIGR01771, L-LDH-NAD, 1 hit |
PROSITEi | View protein in PROSITE PS00064, L_LDH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O93541-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTKEKLISH VMKEEPVGSR SKVTVVGVGM VGMASAISIL LKDLCDELAM
60 70 80 90 100
VDVMEDKLKG EVMDLQHGSL FLKTKIVGDK DYSVTANSKV VVVTAGARQQ
110 120 130 140 150
EGESRLNLVQ RNVNIFKFII PNIVKYSPNC ILMVVSNPVD ILTYVAWKLS
160 170 180 190 200
GFPRHRVIGS GTNLDSARFR HLIGEKLHLH PSSCHAWIVG EHGDSSVPVW
210 220 230 240 250
SGVNVAGVSL QGLNPQMGTE GDGENWKAIH KEVVDGAYEV IKLKGYTSWA
260 270 280 290 300
IGMSVADLVE SIIKNMHKVH PVSTLVQGMH GVKDEVFLSV PCVLGNSGLT
310 320 330
DVIHMTLKAE EEKQLQKSAE TLWGVQKELT L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF079824 mRNA Translation: AAC63282.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF079824 mRNA Translation: AAC63282.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2V65 | X-ray | 2.35 | A/B | 2-331 | [»] | |
SMRi | O93541 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00554;UER00611 |
BRENDAi | 1.1.1.27, 9919 |
Miscellaneous databases
EvolutionaryTracei | O93541 |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
HAMAPi | MF_00488, Lactate_dehydrog, 1 hit |
InterProi | View protein in InterPro IPR001557, L-lactate/malate_DH IPR011304, L-lactate_DH IPR018177, L-lactate_DH_AS IPR022383, Lactate/malate_DH_C IPR001236, Lactate/malate_DH_N IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF02866, Ldh_1_C, 1 hit PF00056, Ldh_1_N, 1 hit |
PIRSFi | PIRSF000102, Lac_mal_DH, 1 hit |
PRINTSi | PR00086, LLDHDRGNASE |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
TIGRFAMsi | TIGR01771, L-LDH-NAD, 1 hit |
PROSITEi | View protein in PROSITE PS00064, L_LDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LDHA_CHAGU | |
Accessioni | O93541Primary (citable) accession number: O93541 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 5, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 105 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families