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Protein

GRB2-related adaptor protein 2

Gene

Grap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

Enzyme and pathway databases

ReactomeiR-MMU-1433557 Signaling by SCF-KIT
R-MMU-202433 Generation of second messenger molecules
R-MMU-2424491 DAP12 signaling
R-MMU-2871796 FCERI mediated MAPK activation
R-MMU-2871809 FCERI mediated Ca+2 mobilization
R-MMU-389356 CD28 co-stimulation

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-related adaptor protein 2
Alternative name(s):
Adapter protein GRID
GADS protein
GRB-2-like protein
Short name:
GRB2L
GRB-2-related monocytic adapter protein
Short name:
MONA
Short name:
Monocytic adapter
GRBLG
Growth factor receptor-binding protein
Hematopoietic cell-associated adaptor protein GrpL
Gene namesi
Name:Grap2
Synonyms:Gads, Grb2l, Grid, Mona
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1333842 Grap2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000882091 – 322GRB2-related adaptor protein 2Add BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45PhosphotyrosineBy similarity1
Modified residuei106N6-acetyllysineBy similarity1
Modified residuei186PhosphoserineCombined sources1
Modified residuei230PhosphoserineCombined sources1
Modified residuei254PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO89100
PaxDbiO89100
PRIDEiO89100

PTM databases

iPTMnetiO89100
PhosphoSitePlusiO89100

Expressioni

Gene expression databases

BgeeiENSMUSG00000042351 Expressed in 42 organ(s), highest expression level in blood
CleanExiMM_GRAP2
ExpressionAtlasiO89100 baseline and differential
GenevisibleiO89100 MM

Interactioni

Subunit structurei

Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB. Interacts with PTPN23 (By similarity). Interacts with phosphorylated LIME1 upon TCR activation.By similarity1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi201466, 9 interactors
DIPiDIP-41343N
IntActiO89100, 15 interactors
MINTiO89100
STRINGi10090.ENSMUSP00000046532

Structurei

Secondary structure

1322
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO89100
SMRiO89100
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO89100

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 56SH3 1PROSITE-ProRule annotationAdd BLAST56
Domaini58 – 149SH2PROSITE-ProRule annotationAdd BLAST92
Domaini263 – 322SH3 2PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3601 Eukaryota
ENOG410XR1G LUCA
GeneTreeiENSGT00820000126999
HOGENOMiHOG000251625
HOVERGENiHBG005404
InParanoidiO89100
KOiK07366
OMAiAKFDFMA
OrthoDBiEOG091G0HWS
PhylomeDBiO89100
TreeFamiTF354288

Family and domain databases

CDDicd11950 SH3_GRAP2_C, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR035646 GRAP2_C_SH3
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF00018 SH3_1, 2 hits
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 2 hits
SUPFAMiSSF50044 SSF50044, 2 hits
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
PS50002 SH3, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O89100-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEATAKFDFM ASGEDELSFR TGDILKILSN QEEWLKAELG SQEGYVPKNF
60 70 80 90 100
IDIEFPEWFH EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED
110 120 130 140 150
DVQHFKVMRD TKGNYFLWTE KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ
160 170 180 190 200
DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS VNRKLSDHLP LGPQQFHPHQ
210 220 230 240 250
QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL GSEVNATLMH
260 270 280 290 300
RRHTDPVQLQ AAGRVRWARA LYDFEALEED ELGFRSGEVV EVLDSSNPSW
310 320
WTGRLHNKLG LFPANYVAPM MR
Length:322
Mass (Da):36,810
Last modified:November 1, 1998 - v1
Checksum:i736311D0640CD3D0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8VH91A0A2R8VH91_MOUSE
GRB2-related adaptor protein 2
Grap2
85Annotation score:
A0A2R8VHI5A0A2R8VHI5_MOUSE
GRB2-related adaptor protein 2
Grap2
53Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055465 mRNA Translation: AAD08803.1
AF053405 mRNA Translation: AAC98669.1
AF129477 mRNA Translation: AAD41783.1
AF236118 mRNA Translation: AAF60318.1
AJ011735 mRNA Translation: CAA09756.1
BC052496 mRNA Translation: AAH52496.1
CCDSiCCDS27663.1
RefSeqiNP_001276371.1, NM_001289442.1
NP_034945.1, NM_010815.3
UniGeneiMm.12947

Genome annotation databases

EnsembliENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351
ENSMUST00000229980; ENSMUSP00000155681; ENSMUSG00000042351
GeneIDi17444
KEGGimmu:17444
UCSCiuc007wvo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055465 mRNA Translation: AAD08803.1
AF053405 mRNA Translation: AAC98669.1
AF129477 mRNA Translation: AAD41783.1
AF236118 mRNA Translation: AAF60318.1
AJ011735 mRNA Translation: CAA09756.1
BC052496 mRNA Translation: AAH52496.1
CCDSiCCDS27663.1
RefSeqiNP_001276371.1, NM_001289442.1
NP_034945.1, NM_010815.3
UniGeneiMm.12947

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3HNMR-A263-322[»]
1OEBX-ray1.76A/B265-322[»]
1R1PX-ray1.80A/B/C/D50-147[»]
1R1QX-ray1.80A/B50-147[»]
1R1SX-ray1.90A/C/E/G50-147[»]
1UTIX-ray1.50A265-322[»]
2D0NX-ray1.57A/C267-322[»]
2W10X-ray1.90A/B265-322[»]
ProteinModelPortaliO89100
SMRiO89100
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201466, 9 interactors
DIPiDIP-41343N
IntActiO89100, 15 interactors
MINTiO89100
STRINGi10090.ENSMUSP00000046532

PTM databases

iPTMnetiO89100
PhosphoSitePlusiO89100

Proteomic databases

EPDiO89100
PaxDbiO89100
PRIDEiO89100

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043149; ENSMUSP00000046532; ENSMUSG00000042351
ENSMUST00000229980; ENSMUSP00000155681; ENSMUSG00000042351
GeneIDi17444
KEGGimmu:17444
UCSCiuc007wvo.2 mouse

Organism-specific databases

CTDi9402
MGIiMGI:1333842 Grap2

Phylogenomic databases

eggNOGiKOG3601 Eukaryota
ENOG410XR1G LUCA
GeneTreeiENSGT00820000126999
HOGENOMiHOG000251625
HOVERGENiHBG005404
InParanoidiO89100
KOiK07366
OMAiAKFDFMA
OrthoDBiEOG091G0HWS
PhylomeDBiO89100
TreeFamiTF354288

Enzyme and pathway databases

ReactomeiR-MMU-1433557 Signaling by SCF-KIT
R-MMU-202433 Generation of second messenger molecules
R-MMU-2424491 DAP12 signaling
R-MMU-2871796 FCERI mediated MAPK activation
R-MMU-2871809 FCERI mediated Ca+2 mobilization
R-MMU-389356 CD28 co-stimulation

Miscellaneous databases

EvolutionaryTraceiO89100
PROiPR:O89100
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042351 Expressed in 42 organ(s), highest expression level in blood
CleanExiMM_GRAP2
ExpressionAtlasiO89100 baseline and differential
GenevisibleiO89100 MM

Family and domain databases

CDDicd11950 SH3_GRAP2_C, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR035646 GRAP2_C_SH3
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF00018 SH3_1, 2 hits
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 2 hits
SUPFAMiSSF50044 SSF50044, 2 hits
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
PS50002 SH3, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGRAP2_MOUSE
AccessioniPrimary (citable) accession number: O89100
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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