UniProtKB - O89090 (SP1_MOUSE)
Transcription factor Sp1
Sp1
Functioni
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter Plays an essential role in the regulation of FE65 gene expression (By similarity).
Positively regulates the transcription of the core clock component ARNTL/BMAL1 (PubMed:24030830).
Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (PubMed:27918959).
By similarity2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 627 – 656 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 657 – 686 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 687 – 714 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 28 |
GO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor binding Source: MGI
- double-stranded DNA binding Source: MGI
- histone acetyltransferase binding Source: MGI
- histone deacetylase binding Source: MGI
- HMG box domain binding Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- protein C-terminus binding Source: MGI
- protein homodimerization activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: BHF-UCL
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: MGI
- transcription coregulator binding Source: MGI
GO - Biological processi
- cellular response to insulin stimulus Source: MGI
- definitive hemopoiesis Source: MGI
- embryonic camera-type eye morphogenesis Source: MGI
- embryonic placenta development Source: MGI
- embryonic process involved in female pregnancy Source: MGI
- embryonic skeletal system development Source: MGI
- enucleate erythrocyte differentiation Source: MGI
- in utero embryonic development Source: MGI
- liver development Source: MGI
- lung development Source: MGI
- megakaryocyte differentiation Source: MGI
- mRNA transcription by RNA polymerase II Source: MGI
- ossification Source: MGI
- positive regulation by host of viral transcription Source: MGI
- positive regulation of amyloid-beta formation Source: MGI
- positive regulation of angiogenesis Source: MGI
- positive regulation of blood vessel endothelial cell migration Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of hydrogen sulfide biosynthetic process Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: ARUK-UCL
- positive regulation of vascular endothelial cell proliferation Source: MGI
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: MGI
- response to hydroperoxide Source: UniProtKB
- rhythmic process Source: UniProtKB-KW
- trophectodermal cell differentiation Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Biological rhythms, Host-virus interaction, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription R-MMU-6807505, RNA polymerase II transcribes snRNA genes R-MMU-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor Sp1Alternative name(s): Specificity protein 11 Publication |
Gene namesi | Name:Sp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98372, Sp1 |
VEuPathDBi | HostDB:ENSMUSG00000001280 |
Subcellular locationi
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- chromatin Source: BHF-UCL
- cytoplasm Source: UniProtKB-SubCell
- euchromatin Source: MGI
- protein-DNA complex Source: MGI
- transcription repressor complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000047138 | 2 – 784 | Transcription factor Sp1Add BLAST | 783 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 7 | PhosphoserineBy similarity | 1 | |
Cross-linki | 16 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 16 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 61 | PhosphoserineCombined sources | 1 | |
Modified residuei | 103 | Phosphoserine; by ATMBy similarity | 1 | |
Modified residuei | 280 | Phosphothreonine; by MAPK8By similarity | 1 | |
Modified residuei | 455 | Phosphothreonine; by MAPK1 and MAPK3By similarity | 1 | |
Glycosylationi | 493 | O-linked (GlcNAc) serineBy similarity | 1 | |
Modified residuei | 613 | Phosphoserine; alternateBy similarity | 1 | |
Glycosylationi | 613 | O-linked (GlcNAc) serine; alternateBy similarity | 1 | |
Modified residuei | 641 | Phosphothreonine; alternateBy similarity | 1 | |
Glycosylationi | 641 | O-linked (GlcNAc) threonine; alternateBy similarity | 1 | |
Modified residuei | 642 | Phosphoserine; by PKC/PRKCZ; alternateBy similarity | 1 | |
Glycosylationi | 642 | O-linked (GlcNAc) serine; alternateBy similarity | 1 | |
Modified residuei | 652 | Phosphothreonine; by PKC/PRKCZBy similarity | 1 | |
Modified residuei | 669 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 671 | Phosphoserine; by PKC/PRKCZBy similarity | 1 | |
Modified residuei | 682 | Phosphothreonine; by PKC/PRKCZBy similarity | 1 | |
Modified residuei | 699 | Phosphoserine; alternateBy similarity | 1 | |
Glycosylationi | 699 | O-linked (GlcNAc) serine; alternateBy similarity | 1 | |
Modified residuei | 703 | Phosphoserine; alternateBy similarity | 1 | |
Glycosylationi | 703 | O-linked (GlcNAc) serine; alternateBy similarity | 1 | |
Modified residuei | 704 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 738 | Phosphothreonine; by MAPK1, MAPK3 and MAPK8By similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 65 – 66 | CleavageBy similarity | 2 |
Keywords - PTMi
Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O89090 |
MaxQBi | O89090 |
PaxDbi | O89090 |
PeptideAtlasi | O89090 |
PRIDEi | O89090 |
ProteomicsDBi | 261485 [O89090-1] 261486 [O89090-2] |
PTM databases
GlyGeni | O89090, 6 sites |
iPTMneti | O89090 |
PhosphoSitePlusi | O89090 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000001280, Expressed in rostral migratory stream and 323 other tissues |
ExpressionAtlasi | O89090, baseline and differential |
Genevisiblei | O89090, MM |
Interactioni
Subunit structurei
Interacts with ATF7IP, ATF7IP2, BAHD1, POGZ, HCFC1, AATF and PHC2.
Interacts with SV40 VP2/3 proteins.
Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the 2 proteins in DNA binding.
Interacts with HLTF; the interaction may be required for basal transcriptional activity of HLTF.
Interacts (deacetylated form) with EP300; the interaction enhances gene expression.
Interacts with HDAC1 and JUN.
Interacts with ELF1; the interaction is inhibited by glycosylation of SP1. Interaction with NFYA; the interaction is inhibited by glycosylation of SP1.
Interacts with SMARCA4/BRG1.
Interacts with ATF7IP and TBP.
Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a.
Interacts with EGR1 (By similarity).
Interacts with RNF112 in an oxidative stress-regulated manner (PubMed:27918959).
Interacts with ZBTB7A; ZBTB7A prevents the binding to GC-rich motifs in promoters and represses the transcriptional activity of SP1 (By similarity).
Interacts with DDX3X; this interaction potentiates SP1-induced CDKN1A/WAF1/CIP1 transcription (By similarity).
By similarity1 Publication(Microbial infection) Interacts with murine minute virus NS1; this interaction allows high levels of viral P38 promoter transactivation by NS1.
2 PublicationsGO - Molecular functioni
- bHLH transcription factor binding Source: BHF-UCL
- DNA-binding transcription factor binding Source: MGI
- histone acetyltransferase binding Source: MGI
- histone deacetylase binding Source: MGI
- HMG box domain binding Source: MGI
- identical protein binding Source: MGI
- protein C-terminus binding Source: MGI
- protein homodimerization activity Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: BHF-UCL
- transcription coregulator binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203414, 29 interactors |
CORUMi | O89090 |
DIPi | DIP-35276N |
IntActi | O89090, 11 interactors |
MINTi | O89090 |
STRINGi | 10090.ENSMUSP00000001326 |
Miscellaneous databases
RNActi | O89090, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 95 | DisorderedSequence analysisAdd BLAST | 95 | |
Regioni | 2 – 84 | Repressor domainBy similarityAdd BLAST | 83 | |
Regioni | 111 – 144 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 148 – 253 | Transactivation domain A (Gln-rich)By similarityAdd BLAST | 106 | |
Regioni | 263 – 497 | Transactivation domain B (Gln-rich)By similarityAdd BLAST | 235 | |
Regioni | 282 – 303 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 333 – 398 | DisorderedSequence analysisAdd BLAST | 66 | |
Regioni | 498 – 611 | Transactivation domain C (highly charged)By similarityAdd BLAST | 114 | |
Regioni | 566 – 598 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 620 – 784 | VZV IE62-bindingBy similarityAdd BLAST | 165 | |
Regioni | 709 – 784 | Domain DBy similarityAdd BLAST | 76 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 464 – 472 | 9aaTADBy similarity | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 16 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 30 – 65 | Polar residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 72 – 95 | Polar residuesSequence analysisAdd BLAST | 24 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 627 – 656 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 657 – 686 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 687 – 714 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 28 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157804 |
HOGENOMi | CLU_019688_1_0_1 |
InParanoidi | O89090 |
OrthoDBi | 1085860at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSDQDHSMDE VTAVVKIEKD VGGNNGGSGN GGGAAFSQTR SSSTGSSSSS
60 70 80 90 100
GGGGGQESQP SPLALLAATC SRIESPNENS NNSQGPSQSG GTGELDLTAT
110 120 130 140 150
QLSQGANGWQ IISSSSGATP TSKEQSGNST NGSNGSESSK NRTVSGGQYV
160 170 180 190 200
VAATPNLQNQ QVLTGLPGVM PNIQYQVIPQ FQTVDGQQLQ FAATGAQVQQ
210 220 230 240 250
DGSGQIQIIP GANQQIIPNR GSGGNIIAAM PNLLQQAVPL QGLANNVLSG
260 270 280 290 300
QTQYVTNVPV ALNGNITLLP VNSVSAATLT PSSQAGTISS SGSQESSSQP
310 320 330 340 350
VTSGTAISSA SLVSSQASSS SFFTNANSYS TTTTTSNMGI MNFTSSGSSG
360 370 380 390 400
TSSQGQTPQR VGGLQGSDSL NIQQNQTSGG SLQGSQQKEG EQSQQTQQQQ
410 420 430 440 450
ILIQPQLVQG GQALQALQAA PLSGQTFTTQ AISQETLQNL QLQAVQNSGP
460 470 480 490 500
IIIRTPTVGP NGQVSWQTLQ LQNLQVQNPQ AQTITLAPMQ GVSLGQTSSS
510 520 530 540 550
NTTLTPIASA ASIPAGTVTV NAAQLSSMPG LQTINLSALG TSGIQVHQLP
560 570 580 590 600
GLPLAIANTP GDHGTQLGLH GSGGDGIHDE TAGGEGENSS DLQPQAGRRT
610 620 630 640 650
RREACTCPYC KDSEGRASGD PGKKKQHICH IQGCGKVYGK TSHLRAHLRW
660 670 680 690 700
HTGERPFMCN WSYCGKRFTR SDELQRHKRT HTGEKKFACP ECPKRFMRSD
710 720 730 740 750
HLSKHIKTHQ NKKGGPGVAL SVGTLPLDSG AGSEGTATPS ALITTNMVAM
760 770 780
EAICPEGIAR LANSGINVMQ VTELQSINIS GNGF
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3X8Q0 | G3X8Q0_MOUSE | Transcription factor Sp1 | Sp1 | 781 | Annotation score: | ||
E9Q113 | E9Q113_MOUSE | Transcription factor Sp1 | Sp1 | 183 | Annotation score: | ||
E9Q2V3 | E9Q2V3_MOUSE | Transcription factor Sp1 | Sp1 | 105 | Annotation score: | ||
E9Q0S7 | E9Q0S7_MOUSE | Transcription factor Sp1 | Sp1 | 55 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 100 | T → A in AAC16484 (PubMed:9628590).Curated | 1 | |
Sequence conflicti | 131 – 133 | Missing in AAC16484 (PubMed:9628590).Curated | 3 | |
Sequence conflicti | 220 | R → E in AAC08527 (Ref. 3) Curated | 1 | |
Sequence conflicti | 462 | G → V in AAC16484 (PubMed:9628590).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007376 | 56 – 372 | Missing in isoform 2. 1 PublicationAdd BLAST | 317 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF062566 mRNA Translation: AAC16484.1 S79832 mRNA Translation: AAB35321.1 AF022363 mRNA Translation: AAC08527.1 X60136 Genomic DNA Translation: CAA42721.1 |
PIRi | S30493 |
RefSeqi | NP_038700.2, NM_013672.2 |
Genome annotation databases
Ensembli | ENSMUST00000163709; ENSMUSP00000130747; ENSMUSG00000001280 [O89090-2] |
GeneIDi | 20683 |
KEGGi | mmu:20683 |
UCSCi | uc012aab.1, mouse [O89090-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF062566 mRNA Translation: AAC16484.1 S79832 mRNA Translation: AAB35321.1 AF022363 mRNA Translation: AAC08527.1 X60136 Genomic DNA Translation: CAA42721.1 |
PIRi | S30493 |
RefSeqi | NP_038700.2, NM_013672.2 |
3D structure databases
SMRi | O89090 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203414, 29 interactors |
CORUMi | O89090 |
DIPi | DIP-35276N |
IntActi | O89090, 11 interactors |
MINTi | O89090 |
STRINGi | 10090.ENSMUSP00000001326 |
PTM databases
GlyGeni | O89090, 6 sites |
iPTMneti | O89090 |
PhosphoSitePlusi | O89090 |
Proteomic databases
EPDi | O89090 |
MaxQBi | O89090 |
PaxDbi | O89090 |
PeptideAtlasi | O89090 |
PRIDEi | O89090 |
ProteomicsDBi | 261485 [O89090-1] 261486 [O89090-2] |
Protocols and materials databases
Antibodypediai | 892, 1068 antibodies from 47 providers |
DNASUi | 20683 |
Genome annotation databases
Ensembli | ENSMUST00000163709; ENSMUSP00000130747; ENSMUSG00000001280 [O89090-2] |
GeneIDi | 20683 |
KEGGi | mmu:20683 |
UCSCi | uc012aab.1, mouse [O89090-2] |
Organism-specific databases
CTDi | 6667 |
MGIi | MGI:98372, Sp1 |
VEuPathDBi | HostDB:ENSMUSG00000001280 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000157804 |
HOGENOMi | CLU_019688_1_0_1 |
InParanoidi | O89090 |
OrthoDBi | 1085860at2759 |
Enzyme and pathway databases
Reactomei | R-MMU-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription R-MMU-6807505, RNA polymerase II transcribes snRNA genes R-MMU-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
BioGRID-ORCSi | 20683, 21 hits in 68 CRISPR screens |
ChiTaRSi | Sp1, mouse |
PROi | PR:O89090 |
RNActi | O89090, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001280, Expressed in rostral migratory stream and 323 other tissues |
ExpressionAtlasi | O89090, baseline and differential |
Genevisiblei | O89090, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SP1_MOUSE | |
Accessioni | O89090Primary (citable) accession number: O89090 Secondary accession number(s): O89087, Q62251, Q64167 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 9, 2003 |
Last sequence update: | June 21, 2005 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families