Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Muskelin

Gene

Mkln1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (By similarity). Required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent GABRA1 degradation (PubMed:21482357). Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1 (PubMed:9724633, PubMed:18710924).By similarity3 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Muskelin
Gene namesi
Name:Mkln1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1351638 Mkln1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Nucleus, Synapse

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Hippocampus slices from mutant mice show minor differences in the amplitude of miniature inhibitory postsynaptic currents, and somewhat slower decay times.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi182 – 183KH → AA: Abolishes targeting to the nucleus. 1 Publication2
Mutagenesisi701 – 735Missing : Strongly increased location in the nucleus. 1 PublicationAdd BLAST35
Mutagenesisi723T → A: No effect on predominant location in the cytosol. 1 Publication1
Mutagenesisi723T → D: Strongly increased location in the nucleus. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001191392 – 735MuskelinAdd BLAST734

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO89050
MaxQBiO89050
PaxDbiO89050
PeptideAtlasiO89050
PRIDEiO89050

PTM databases

iPTMnetiO89050
PhosphoSitePlusiO89050

Expressioni

Tissue specificityi

Detected in brain, especially in hippocampus and cerebellum (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000025609 Expressed in 292 organ(s), highest expression level in female gonad
ExpressionAtlasiO89050 baseline and differential
GenevisibleiO89050 MM

Interactioni

Subunit structurei

Homodimer; may form higher oligomers (By similarity). Identified in the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5. Within this complex, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, WDR26, and RANBP9 and/or RANBP10 form the catalytic core, while GID4, MKLN1, ARMC8 and YPEL5 have ancillary roles (By similarity). Interacts with RANBP9 (PubMed:18710924). Part of a complex consisting of RANBP9, MKLN1 and GID8 (By similarity). Interacts with GABRA1 (PubMed:21482357). Interacts with the C-terminal tail of PTGER3 (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-11017656,EBI-11017656

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205222, 8 interactors
DIPiDIP-61380N
IntActiO89050, 7 interactors
STRINGi10090.ENSMUSP00000026699

Structurei

Secondary structure

1735
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO89050
SMRiO89050
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 204LisHPROSITE-ProRule annotationAdd BLAST33
Domaini206 – 258CTLHPROSITE-ProRule annotationAdd BLAST53
Repeati284 – 330Kelch 1Add BLAST47
Repeati339 – 391Kelch 2Add BLAST53
Repeati400 – 458Kelch 3Add BLAST59
Repeati469 – 515Kelch 4Add BLAST47
Repeati526 – 578Kelch 5Add BLAST53
Repeati597 – 651Kelch 6Add BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni701 – 735Important for location in the cytosol1 PublicationAdd BLAST35

Domaini

The LisH domain contains a nuclear targeting signal (PubMed:18710924). The LisH domain mediates head to tail dimerization (By similarity).By similarity1 Publication

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG2437 Eukaryota
ENOG410YJKC LUCA
GeneTreeiENSGT00390000001702
HOGENOMiHOG000047912
HOVERGENiHBG052481
InParanoidiO89050
OMAiWYVELNG
OrthoDBiEOG091G07E6
PhylomeDBiO89050
TreeFamiTF323659

Family and domain databases

Gene3Di2.120.10.80, 2 hits
2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR006595 CTLH_C
IPR011043 Gal_Oxase/kelch_b-propeller
IPR008979 Galactose-bd-like_sf
IPR015915 Kelch-typ_b-propeller
IPR006652 Kelch_1
IPR006594 LisH
IPR010565 Muskelin_N
PfamiView protein in Pfam
PF01344 Kelch_1, 1 hit
PF06588 Muskelin_N, 1 hit
SMARTiView protein in SMART
SM00667 LisH, 1 hit
SUPFAMiSSF117281 SSF117281, 1 hit
SSF49785 SSF49785, 1 hit
SSF50965 SSF50965, 1 hit
PROSITEiView protein in PROSITE
PS50897 CTLH, 1 hit
PS50896 LISH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O89050-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAGGAVAVA PECRLLPYAL HKWSSFSSTY LPENILVDKP NDQSSRWSSE
60 70 80 90 100
SNYPPQYLIL KLERPAIVQN ITFGKYEKTH VCNLKKFKVF GGMNEENMTE
110 120 130 140 150
LLSSGLKNDY NKETFTLKHK IDEQMFPCRF IKIVPLLSWG PSFNFSIWYV
160 170 180 190 200
ELSGIDDPDI VQPCLNWYSK YREQEAIRLC LKHFRQHNYT EAFESLQKKT
210 220 230 240 250
KIALEHPMLT DMHDKLVLKG DFDACEELIE KAVNDGLFNQ YISQQEYKPR
260 270 280 290 300
WSQIIPKSTK GDGEDNRPGM RGGHQMVIDV QTETVYLFGG WDGTQDLADF
310 320 330 340 350
WAYSVKENQW TCISRDTEKE NGPSARSCHK MCIDIQRRQI YTLGRYLDSS
360 370 380 390 400
VRNSKSLKSD FYRYDIDTNT WMLLSEDTAA DGGPKLVFDH QMCMDSEKHM
410 420 430 440 450
IYTFGGRILT CNGSVDDSRA SEPQFSGLFA FNCQCQTWKL LREDSCNAGP
460 470 480 490 500
EDIQSRIGHC MLFHSKNRCL YVFGGQRSKT YLNDFFSYDV DSDHVDIISD
510 520 530 540 550
GTKKDSGMVP MTGFTQRATI DPELNEIHVL SGLSKDKEKR EENVRNSFWI
560 570 580 590 600
YDIVRNSWSC VYKNDQATKD NLSKSLQEEE PCPRFAHQLV YDELHKVHYL
610 620 630 640 650
FGGNPGKSCS PKMRLDDFWS LKLCRPSKDY LLRHCKYLIR KHRFEEKAQM
660 670 680 690 700
DPLSALKYLQ NDLYITVDHS DPEETKEFQL LASALFKSGS DFTALGFSDV
710 720 730
DHTYAQRTQL FDTLVNFFPD SMTPPKGNLV DLITL
Length:735
Mass (Da):84,878
Last modified:November 1, 1998 - v1
Checksum:i7B0C8D2CB8C242AA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6UW85F6UW85_MOUSE
Muskelin
Mkln1
167Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72194 mRNA Translation: AAC63849.1
BC013703 mRNA Translation: AAH13703.1
CCDSiCCDS19982.1
RefSeqiNP_038819.1, NM_013791.2
UniGeneiMm.238038
Mm.392838
Mm.440531

Genome annotation databases

EnsembliENSMUST00000026699; ENSMUSP00000026699; ENSMUSG00000025609
GeneIDi27418
KEGGimmu:27418
UCSCiuc009bgf.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72194 mRNA Translation: AAC63849.1
BC013703 mRNA Translation: AAH13703.1
CCDSiCCDS19982.1
RefSeqiNP_038819.1, NM_013791.2
UniGeneiMm.238038
Mm.392838
Mm.440531

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PQQX-ray1.55A12-167[»]
ProteinModelPortaliO89050
SMRiO89050
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205222, 8 interactors
DIPiDIP-61380N
IntActiO89050, 7 interactors
STRINGi10090.ENSMUSP00000026699

PTM databases

iPTMnetiO89050
PhosphoSitePlusiO89050

Proteomic databases

EPDiO89050
MaxQBiO89050
PaxDbiO89050
PeptideAtlasiO89050
PRIDEiO89050

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026699; ENSMUSP00000026699; ENSMUSG00000025609
GeneIDi27418
KEGGimmu:27418
UCSCiuc009bgf.1 mouse

Organism-specific databases

CTDi4289
MGIiMGI:1351638 Mkln1

Phylogenomic databases

eggNOGiKOG2437 Eukaryota
ENOG410YJKC LUCA
GeneTreeiENSGT00390000001702
HOGENOMiHOG000047912
HOVERGENiHBG052481
InParanoidiO89050
OMAiWYVELNG
OrthoDBiEOG091G07E6
PhylomeDBiO89050
TreeFamiTF323659

Miscellaneous databases

ChiTaRSiMkln1 mouse
PROiPR:O89050
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025609 Expressed in 292 organ(s), highest expression level in female gonad
ExpressionAtlasiO89050 baseline and differential
GenevisibleiO89050 MM

Family and domain databases

Gene3Di2.120.10.80, 2 hits
2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR006595 CTLH_C
IPR011043 Gal_Oxase/kelch_b-propeller
IPR008979 Galactose-bd-like_sf
IPR015915 Kelch-typ_b-propeller
IPR006652 Kelch_1
IPR006594 LisH
IPR010565 Muskelin_N
PfamiView protein in Pfam
PF01344 Kelch_1, 1 hit
PF06588 Muskelin_N, 1 hit
SMARTiView protein in SMART
SM00667 LisH, 1 hit
SUPFAMiSSF117281 SSF117281, 1 hit
SSF49785 SSF49785, 1 hit
SSF50965 SSF50965, 1 hit
PROSITEiView protein in PROSITE
PS50897 CTLH, 1 hit
PS50896 LISH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMKLN1_MOUSE
AccessioniPrimary (citable) accession number: O89050
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again