UniProtKB - O89042 (DPOLA_RAT)
DNA polymerase alpha catalytic subunit
Pola1
Functioni
Miscellaneous
Catalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1290 | ZincBy similarity | 1 | |
Metal bindingi | 1293 | ZincBy similarity | 1 | |
Metal bindingi | 1317 | ZincBy similarity | 1 | |
Metal bindingi | 1322 | ZincBy similarity | 1 | |
Metal bindingi | 1355 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 1360 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 1378 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 1381 | Iron-sulfur (4Fe-4S)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1290 – 1320 | CysA-typeAdd BLAST | 31 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- chromatin binding Source: UniProtKB
- DNA binding Source: UniProtKB
- DNA-directed DNA polymerase activity Source: RGD
- DNA replication origin binding Source: GO_Central
- double-stranded DNA binding Source: RGD
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB
- protein kinase binding Source: RGD
- purine nucleotide binding Source: RGD
- pyrimidine nucleotide binding Source: RGD
- single-stranded DNA binding Source: GO_Central
GO - Biological processi
- DNA biosynthetic process Source: RGD
- DNA repair Source: RGD
- DNA replication Source: RGD
- DNA replication, synthesis of RNA primer Source: UniProtKB
- DNA replication initiation Source: UniProtKB
- DNA strand elongation involved in DNA replication Source: UniProtKB
- DNA synthesis involved in DNA repair Source: RGD
- double-strand break repair via nonhomologous end joining Source: UniProtKB
- lagging strand elongation Source: UniProtKB
- leading strand elongation Source: UniProtKB
- mitotic DNA replication initiation Source: GO_Central
- regulation of type I interferon production Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase alpha catalytic subunit (EC:2.7.7.71 Publication)Alternative name(s): DNA polymerase alpha catalytic subunit p180 |
Gene namesi | Name:Pola1 Synonyms:Pola |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621816, Pola1 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Nucleus
- alpha DNA polymerase:primase complex Source: RGD
- nuclear envelope Source: UniProtKB
- nuclear matrix Source: UniProtKB
- nucleolus Source: UniProtKB
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046430 | 1 – ›1451 | DNA polymerase alpha catalytic subunitAdd BLAST | ›1451 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 192 | PhosphoserineBy similarity | 1 | |
Modified residuei | 196 | PhosphoserineBy similarity | 1 | |
Modified residuei | 215 | PhosphoserineBy similarity | 1 | |
Modified residuei | 230 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 413 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 977 | N6-succinyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | O89042 |
PaxDbi | O89042 |
PRIDEi | O89042 |
PTM databases
iPTMneti | O89042 |
PhosphoSitePlusi | O89042 |
Interactioni
Subunit structurei
Component of the alpha DNA polymerase complex (also known as the alpha DNA polymerase-primase complex) consisting of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the primase complex subunits PRIM1 and PRIM2 respectively.
Interacts with PARP1; this interaction functions as part of the control of replication fork progression.
Interacts with MCM10 and WDHD1; these interactions recruit the polymerase alpha complex to the pre-replicative complex bound to DNA.
Interacts with RPA1; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp (By similarity).
By similarityGO - Molecular functioni
- protein kinase binding Source: RGD
Protein-protein interaction databases
ComplexPortali | CPX-2089, DNA polymerase alpha:primase complex |
STRINGi | 10116.ENSRNOP00000018147 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 657 – 722 | DNA-bindingSequence analysisAdd BLAST | 66 | |
Regioni | 1252 – 1383 | DNA-bindingSequence analysisAdd BLAST | 132 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1355 – 1381 | CysB motifAdd BLAST | 27 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1290 – 1320 | CysA-typeAdd BLAST | 31 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0970, Eukaryota |
InParanoidi | O89042 |
OrthoDBi | 293315at2759 |
PhylomeDBi | O89042 |
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 1.10.3200.20, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR024647, DNA_pol_a_cat_su_N IPR042087, DNA_pol_B_C IPR023211, DNA_pol_palm_dom_sf IPR038256, Pol_alpha_znc_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf IPR015088, Znf_DNA-dir_DNA_pol_B_alpha |
Pfami | View protein in Pfam PF12254, DNA_pol_alpha_N, 1 hit PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 1 hit PF08996, zf-DNA_Pol, 1 hit |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
(1+)i Sequence
Sequence statusi: Fragment.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAPVHGDDCK LETSAVSDSG SFVASRARRE KKSKKGRQEA LERLKKAKAG
60 70 80 90 100
EKYKYEVEDL TSVYEEVDEE QYSKLVQARQ DDDWIVDDDG IGYVEDGREI
110 120 130 140 150
FDDDLEDDAL DTCGEGSDGK AHRKDRKDVK KPSVTKPNNI KAMFIASAGK
160 170 180 190 200
KTTDKTVDLS KDDLLGDILQ DLNTETPQIA PPPVLIPKKK RSTGASPNPF
210 220 230 240 250
SVHTATAVPS GKIASPVSRK EPPLTPVPLK RAEFAGDLAQ PECPEDEQES
260 270 280 290 300
GVIEFEDGDF DEPMDTEEVD EEEPVTAKIW DQESEPVEGV KHEADPETGT
310 320 330 340 350
TSFLDSFLPD VSCWDIDQKD ENSFLLQEVQ VDSNHLPLVK GADDEQVFQF
360 370 380 390 400
YWLDAYEDPY NQPGVVFLFG KVWVESAKTH VSCCVMVKNI ERTLYFLPRE
410 420 430 440 450
MKIDLNTGKE TATPITMKDV YEEFDSKISA KYKIMKFKSK IVEKNYAFEI
460 470 480 490 500
PDVPEKSEYL EVRYSAEVPQ LPQNLKGETF SHVFGTNTSS LELFLMNRKI
510 520 530 540 550
KGPCWLEVKN PQLLNQPISW CKFEAMALKP DLVNVIKDVS PPPLVVMSFS
560 570 580 590 600
MKTMQNVQNH QHEIIAMAAL VHHNFPLDKA PPKPPFQTHF CVVSKPKDCI
610 620 630 640 650
FPCAFKEVIK KKNMEVEVAA TERTLLGFFL AKVHKLDPDI LVGHNICGFE
660 670 680 690 700
LEVLLQRINE CKVPFWSKIG RLRRSNMPKL GSRSGFGERN ATCGRMICDV
710 720 730 740 750
EISVKELIHC KSYHLSELVQ QILKTERIVI PTENIRNMYS EPSHLLYLLE
760 770 780 790 800
HIWKDARFIL QIMCELNVLP LALQITNIAG NIMSRTLMGG RSERNEFLLL
810 820 830 840 850
HAFYENNYIV PDKQIFRKPQ QKPGDEDEEI DGDTNKYKKG RKKAAYAGGL
860 870 880 890 900
VLDPKVGFYD KFILLLDFNS LYPSIIQEFN ICFTTVQRVA SETLKATEDE
910 920 930 940 950
EQEQIPELPD PNLDMGILPR EIRKLVERRK QVKQLMKQQD LNPDLVLQYD
960 970 980 990 1000
IRQKALKLTA NSMYGCLGFS YSRFYAKPLA ALVTYKGREI LMHTKEMVQK
1010 1020 1030 1040 1050
MNLEVIYGDT DSIMINTNST NLEEVFKLGN KVKNEVNKLY KLLEIDIDGV
1060 1070 1080 1090 1100
FKSLLLLKKK KYAALVVEPT SDGNYITKQE LKGLDIVRRD WCDLAKDTGN
1110 1120 1130 1140 1150
FVIGQILSDQ SRDTIVENIQ KRLIEIGENV LNGSVPVSQF EINKALTKDP
1160 1170 1180 1190 1200
QDYPDKKSLP HVHVALWINS QGGRKVKAGD TVSYVICQDG SNLPATQRAY
1210 1220 1230 1240 1250
APEQLQKQDN LAIDTQYYLA QQIHPVVARI CEPIDGIDAV LIALWLGLDS
1260 1270 1280 1290 1300
TQFRVHQYHK DEENDALLGG PAQLTDEEKY KDCEKFKCLC PSCGTENIYD
1310 1320 1330 1340 1350
NVFEGSGMDM EPSLNRCSNI DCKASPATFM VQLSNKLIMD IRRCIKKYYD
1360 1370 1380 1390 1400
GWLICEEPTC RNRIRRLPLH FSRNGPLCPA CMKAVLRPEY SDKSLYTQLC
1410 1420 1430 1440 1450
FYRYIFDADC ALEKLPEHEK DKLKKQFFTP RVLQDYRKVK NIAEHFLSWS
G
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LRJ6 | F1LRJ6_RAT | DNA polymerase | Pola1 | 1,340 | Annotation score: | ||
A0A096MK13 | A0A096MK13_RAT | DNA polymerase | Pola1 | 957 | Annotation score: | ||
A0A096MJL5 | A0A096MJL5_RAT | DNA polymerase alpha catalytic subu... | Pola1 | 465 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1451 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ011605 mRNA Translation: CAA09720.1 |
RefSeqi | NP_445931.1, NM_053479.1 |
Genome annotation databases
GeneIDi | 85241 |
KEGGi | rno:85241 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ011605 mRNA Translation: CAA09720.1 |
RefSeqi | NP_445931.1, NM_053479.1 |
3D structure databases
SMRi | O89042 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-2089, DNA polymerase alpha:primase complex |
STRINGi | 10116.ENSRNOP00000018147 |
PTM databases
iPTMneti | O89042 |
PhosphoSitePlusi | O89042 |
Proteomic databases
jPOSTi | O89042 |
PaxDbi | O89042 |
PRIDEi | O89042 |
Genome annotation databases
GeneIDi | 85241 |
KEGGi | rno:85241 |
Organism-specific databases
CTDi | 5422 |
RGDi | 621816, Pola1 |
Phylogenomic databases
eggNOGi | KOG0970, Eukaryota |
InParanoidi | O89042 |
OrthoDBi | 293315at2759 |
PhylomeDBi | O89042 |
Family and domain databases
Gene3Di | 1.10.132.60, 1 hit 1.10.3200.20, 1 hit 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR024647, DNA_pol_a_cat_su_N IPR042087, DNA_pol_B_C IPR023211, DNA_pol_palm_dom_sf IPR038256, Pol_alpha_znc_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf IPR015088, Znf_DNA-dir_DNA_pol_B_alpha |
Pfami | View protein in Pfam PF12254, DNA_pol_alpha_N, 1 hit PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 1 hit PF08996, zf-DNA_Pol, 1 hit |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOLA_RAT | |
Accessioni | O89042Primary (citable) accession number: O89042 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families