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Protein

Roundabout homolog 1

Gene

Robo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10433822, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (By similarity). May be required for lung development (PubMed:11734623).By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Receptor
Biological processChemotaxis, Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Roundabout homolog 1
Gene namesi
Name:Robo1
Synonyms:Dutt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1274781 Robo1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 858ExtracellularSequence analysisAdd BLAST839
Transmembranei859 – 879HelicalSequence analysisAdd BLAST21
Topological domaini880 – 1612CytoplasmicSequence analysisAdd BLAST733

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show defects in commissural axon guidance in spinal chord including midline recrossing and an altered lateral and ventral funiculi projection. The phenotype resembles that of a SLIT1;SLIT2;SLIT3 triple mutant. They also mimick a naturally occurring human homozygous deletion mutant detected in a small lung cancer cell line, frequently die at birth by respiratory failure with accompanying abnormal lung histology. Surviving mice develop bronchial hyperplasia.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003103420 – 1612Roundabout homolog 1Add BLAST1593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 108PROSITE-ProRule annotation
Glycosylationi121N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi152 ↔ 201PROSITE-ProRule annotation
Disulfide bondi244 ↔ 291PROSITE-ProRule annotation
Disulfide bondi333 ↔ 389PROSITE-ProRule annotation
Glycosylationi424N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi437 ↔ 486PROSITE-ProRule annotation
Glycosylationi751N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi781N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi788N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei901PhosphoserineBy similarity1
Modified residuei909PhosphothreonineBy similarity1
Modified residuei999PhosphotyrosineBy similarity1
Modified residuei1016PhosphoserineBy similarity1
Modified residuei1034PhosphotyrosineBy similarity1
Modified residuei1075PhosphotyrosineBy similarity1
Modified residuei1201PhosphothreonineBy similarity1
Modified residuei1258PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated. May be deubiquitinated by USP33.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO89026
PaxDbiO89026
PRIDEiO89026

PTM databases

iPTMnetiO89026
PhosphoSitePlusiO89026

Expressioni

Tissue specificityi

Detected in embryonic thalamus neurons (at protein level) (PubMed:24560577). Expressed in embryonal spinal chord. Expressed in embryonal lung, and in adult lung bronchial epithelial cells of large proximal airways.3 Publications

Developmental stagei

Earliest and highest expression at 11 dpc. Expression is detected in developing somits, brain, neural tube, and pericardiac mesenchyme. By in situ hybridization is detected in marginal zones bordering the mitotically active periventricular region, weakly extending to the ventral aspect impinging on motor neuron columns. Also detected in the ventral third of the developing neural tube, and in spinal chord throughout the full length of the neural tube. Also detected at 17.5 dpc in lung mesenchyme. Expressed at E11.5 in spinal chord, predominantly localized to postcrossing commissural axons.3 Publications

Gene expression databases

CleanExiMM_ROBO1

Interactioni

Subunit structurei

Homodimer. Dimerization is mediated by the extracellular domain and is independent of SLIT liganding (By similarity). Interacts with SLIT1 (PubMed:10433822) Interacts with SLIT2 (By similarity). Interacts with FLRT3 (PubMed:24560577). Interacts with MYO9B (via Rho-GAP domain) (By similarity).By similarity4 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202949, 4 interactors
STRINGi10090.ENSMUSP00000023600

Structurei

3D structure databases

ProteinModelPortaliO89026
SMRiO89026
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 125Ig-like C2-type 1Add BLAST97
Domaini131 – 218Ig-like C2-type 2Add BLAST88
Domaini223 – 307Ig-like C2-type 3Add BLAST85
Domaini312 – 407Ig-like C2-type 4Add BLAST96
Domaini416 – 502Ig-like C2-type 5Add BLAST87
Domaini524 – 618Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST95
Domaini637 – 734Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST98
Domaini739 – 835Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST97

Sequence similaritiesi

Belongs to the immunoglobulin superfamily. ROBO family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4222 Eukaryota
ENOG410XPQS LUCA
HOGENOMiHOG000010267
HOVERGENiHBG073476
InParanoidiO89026
KOiK06753
PhylomeDBiO89026

Family and domain databases

CDDicd00063 FN3, 3 hits
Gene3Di2.60.40.10, 8 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
IPR032986 Robo1
PANTHERiPTHR44096:SF1 PTHR44096:SF1, 1 hit
PfamiView protein in Pfam
PF00041 fn3, 3 hits
PF07679 I-set, 2 hits
SMARTiView protein in SMART
SM00060 FN3, 3 hits
SM00409 IG, 5 hits
SM00408 IGc2, 5 hits
SM00406 IGv, 3 hits
SUPFAMiSSF48726 SSF48726, 5 hits
SSF49265 SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 3 hits
PS50835 IG_LIKE, 5 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O89026-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC
60 70 80 90 100
KAEGRPTPTI EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR
110 120 130 140 150
PDEGVYICVA RNYLGEAVSH NASLEVAILR DDFRQNPSDV MVAVGEPAVM
160 170 180 190 200
ECQPPRGHPE PTISWKKDGS PLDDKDERIT IRGGKLMITY TRKSDAGKYV
210 220 230 240 250
CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA EFKCEARGDP
260 270 280 290 300
VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA
310 320 330 340 350
EASATLTVQE PPHFVVKPRD QVVALGRTVT FQCEATGNPQ PAIFWRREGS
360 370 380 390 400
QNLLFSYQPP QSSSRFSVSQ TGDLTITNVQ RSDVGYYICQ TLNVAGSIIT
410 420 430 440 450
KAYLEVTDVI ADRPPPVIRQ GPVNQTVAVD GTLILSCVAT GSPAPTILWR
460 470 480 490 500
KDGVLVSTQD SRIKQLESGV LQIRYAKLGD TGRYTCTAST PSGEATWSAY
510 520 530 540 550
IEVQEFGVPV QPPRPTDPNL IPSAPSKPEV TDVSKNTVTL SWQPNLNSGA
560 570 580 590 600
TPTSYIIEAF SHASGSSWQT AAENVKTETF AIKGLKPNAI YLFLVRAANA
610 620 630 640 650
YGISDPSQIS DPVKTQDVPP TSQGVDHKQV QRELGNVVLH LHNPTILSSS
660 670 680 690 700
SVEVHWTVDQ QSQYIQGYKI LYRPSGASHG ESEWLVFEVR TPTKNSVVIP
710 720 730 740 750
DLRKGVNYEI KARPFFNEFQ GADSEIKFAK TLEEAPSAPP RSVTVSKNDG
760 770 780 790 800
NGTAILVTWQ PPPEDTQNGM VQEYKVWCLG NETKYHINKT VDGSTFSVVI
810 820 830 840 850
PSLVPGIRYS VEVAASTGAG PGVKSEPQFI QLDSHGNPVS PEDQVSLAQQ
860 870 880 890 900
ISDVVRQPAF IAGIGAACWI ILMVFSIWLY RHRKKRNGLT STYAGIRKVP
910 920 930 940 950
SFTFTPTVTY QRGGEAVSSG GRPGLLNISE PATQPWLADT WPNTGNNHND
960 970 980 990 1000
CSINCCTAGN GNSDSNLTTY SRPADCIANY NNQLDNKQTN LMLPESTVYG
1010 1020 1030 1040 1050
DVDLSNKINE MKTFNSPNLK DGRFVNPSGQ PTPYATTQLI QANLSNNMNN
1060 1070 1080 1090 1100
GAGDSSEKHW KPPGQQKPEV APIQYNIMEQ NKLNKDYRAN DTIPPTIPYN
1110 1120 1130 1140 1150
QSYDQNTGGS YNSSDRGSST SGSQGHKKGA RTPKAPKQGG MNWADLLPPP
1160 1170 1180 1190 1200
PAHPPPHSNS EEYNMSVDES YDQEMPCPVP PAPMYLQQDE LQEEEDERGP
1210 1220 1230 1240 1250
TPPVRGAASS PAAVSYSHQS TATLTPSPQE ELQPMLQDCP EDLGHMPHPP
1260 1270 1280 1290 1300
DRRRQPVSPP PPPRPISPPH TYGYISGPLV SDMDTDAPEE EEDEADMEVA
1310 1320 1330 1340 1350
KMQTRRLLLR GLEQTPASSV GDLESSVTGS MINGWGSASE EDNISSGRSS
1360 1370 1380 1390 1400
VSSSDGSFFT DADFAQAVAA AAEYAGLKVA RRQMQDAAGR RHFHASQCPR
1410 1420 1430 1440 1450
PTSPVSTDSN MSAVVIQKAR PAKKQKHQPG HLRREAYADD LPPPPVPPPA
1460 1470 1480 1490 1500
IKSPTVQSKA QLEVRPVMVP KLASIEARTD RSSDRKGGSY KGREALDGRQ
1510 1520 1530 1540 1550
VTDLRTNPSD PREAQEQPND GKGRGTRQPK RDLPPAKTHL GQEDILPYCR
1560 1570 1580 1590 1600
PTFPTSNNPR DPSSSSSMSS RGSGSRQREQ ANVGRRNMAE MQVLGGFERG
1610
DENNEELEET ES
Length:1,612
Mass (Da):176,407
Last modified:November 1, 1998 - v1
Checksum:i5F2988C544796B4B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E843G5E843_MOUSE
Roundabout homolog 1
Robo1 mCG_142127
1,612Annotation score:
A0A338P6U2A0A338P6U2_MOUSE
Roundabout homolog 1
Robo1
133Annotation score:
A0A338P6F7A0A338P6F7_MOUSE
Roundabout homolog 1
Robo1
1,622Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17793 mRNA Translation: CAA76850.1
CCDSiCCDS37376.1
PIRiT30805
RefSeqiNP_062286.2, NM_019413.2
UniGeneiMm.310772

Genome annotation databases

GeneIDi19876
KEGGimmu:19876

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17793 mRNA Translation: CAA76850.1
CCDSiCCDS37376.1
PIRiT30805
RefSeqiNP_062286.2, NM_019413.2
UniGeneiMm.310772

3D structure databases

ProteinModelPortaliO89026
SMRiO89026
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202949, 4 interactors
STRINGi10090.ENSMUSP00000023600

PTM databases

iPTMnetiO89026
PhosphoSitePlusiO89026

Proteomic databases

MaxQBiO89026
PaxDbiO89026
PRIDEiO89026

Protocols and materials databases

DNASUi19876
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi19876
KEGGimmu:19876

Organism-specific databases

CTDi6091
MGIiMGI:1274781 Robo1

Phylogenomic databases

eggNOGiKOG4222 Eukaryota
ENOG410XPQS LUCA
HOGENOMiHOG000010267
HOVERGENiHBG073476
InParanoidiO89026
KOiK06753
PhylomeDBiO89026

Miscellaneous databases

ChiTaRSiRobo1 mouse
PROiPR:O89026
SOURCEiSearch...

Gene expression databases

CleanExiMM_ROBO1

Family and domain databases

CDDicd00063 FN3, 3 hits
Gene3Di2.60.40.10, 8 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
IPR032986 Robo1
PANTHERiPTHR44096:SF1 PTHR44096:SF1, 1 hit
PfamiView protein in Pfam
PF00041 fn3, 3 hits
PF07679 I-set, 2 hits
SMARTiView protein in SMART
SM00060 FN3, 3 hits
SM00409 IG, 5 hits
SM00408 IGc2, 5 hits
SM00406 IGv, 3 hits
SUPFAMiSSF48726 SSF48726, 5 hits
SSF49265 SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 3 hits
PS50835 IG_LIKE, 5 hits
ProtoNetiSearch...

Entry informationi

Entry nameiROBO1_MOUSE
AccessioniPrimary (citable) accession number: O89026
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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