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Protein

Afamin

Gene

Afm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as carrier for hydrophobic molecules in body fluids. Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Afamin
Alternative name(s):
Alpha-albumin
Short name:
Alpha-Alb
Gene namesi
Name:Afm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2429409 Afm

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000000110722 – 608AfaminAdd BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) asparagine3 Publications1
Disulfide bondi77 ↔ 86PROSITE-ProRule annotation
Disulfide bondi99 ↔ 114PROSITE-ProRule annotation
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi113 ↔ 124PROSITE-ProRule annotation
Disulfide bondi148 ↔ 193PROSITE-ProRule annotation
Glycosylationi153N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi192 ↔ 201PROSITE-ProRule annotation
Disulfide bondi224 ↔ 270PROSITE-ProRule annotation
Disulfide bondi269 ↔ 277PROSITE-ProRule annotation
Disulfide bondi289 ↔ 303PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi340 ↔ 385PROSITE-ProRule annotation
Disulfide bondi384 ↔ 393PROSITE-ProRule annotation
Glycosylationi402N-linked (GlcNAc...) asparagine3 Publications1
Disulfide bondi416 ↔ 462PROSITE-ProRule annotation
Disulfide bondi461 ↔ 470PROSITE-ProRule annotation
Disulfide bondi483 ↔ 499PROSITE-ProRule annotation
Glycosylationi488N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi498 ↔ 509PROSITE-ProRule annotation
Disulfide bondi580 ↔ 589PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated; more than 90% of the glycans are sialylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO89020
PaxDbiO89020
PeptideAtlasiO89020
PRIDEiO89020

PTM databases

iPTMnetiO89020
PhosphoSitePlusiO89020

Expressioni

Tissue specificityi

Detected in brain, especially on brain capillaries (at protein level). Expressed in isolated brain capillaries.1 Publication

Gene expression databases

BgeeiENSMUSG00000029369 Expressed in 78 organ(s), highest expression level in liver
CleanExiMM_AFM
GenevisibleiO89020 MM

Interactioni

Subunit structurei

Forms a 1:1 complex with Wnt family members; interacts with WNT1, WNT2B, WNT3, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (By similarity). Interacts with WNT3A (PubMed:26902720).By similarity1 Publication

Protein-protein interaction databases

IntActiO89020, 1 interactor
MINTiO89020
STRINGi10090.ENSMUSP00000108804

Structurei

3D structure databases

ProteinModelPortaliO89020
SMRiO89020
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 210Albumin 1PROSITE-ProRule annotationAdd BLAST189
Domaini211 – 403Albumin 2PROSITE-ProRule annotationAdd BLAST193
Domaini404 – 599Albumin 3PROSITE-ProRule annotationAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 319Binding pocket for hydrophobic ligandsBy similarityAdd BLAST105

Domaini

The second albumin domain forms a deep binding pocket that contains palmitoleic acid (in vitro). Palmitoleic acid is most likely not the physiological ligand. Instead, this pocket may accomodate the covalently bound lipid moiety of Wnt family members.By similarity

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IIRZ Eukaryota
ENOG410Z40H LUCA
GeneTreeiENSGT00390000000113
HOGENOMiHOG000293137
HOVERGENiHBG004207
InParanoidiO89020
OMAiFACVDNL
OrthoDBiEOG091G0F5F
PhylomeDBiO89020
TreeFamiTF335561

Family and domain databases

CDDicd00015 ALBUMIN, 3 hits
InterProiView protein in InterPro
IPR000264 ALB/AFP/VDB
IPR020858 Serum_albumin-like
IPR021177 Serum_albumin/AFP/Afamin
IPR020857 Serum_albumin_CS
IPR014760 Serum_albumin_N
PANTHERiPTHR11385 PTHR11385, 1 hit
PfamiView protein in Pfam
PF00273 Serum_albumin, 3 hits
PIRSFiPIRSF002520 Serum_albumin_subgroup, 1 hit
PRINTSiPR00803 AFETOPROTEIN
PR00802 SERUMALBUMIN
SMARTiView protein in SMART
SM00103 ALBUMIN, 3 hits
SUPFAMiSSF48552 SSF48552, 3 hits
PROSITEiView protein in PROSITE
PS00212 ALBUMIN_1, 2 hits
PS51438 ALBUMIN_2, 3 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O89020-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRHLKLTGFI FFLLPLTESL ALPTKPQDVD HFNATQKFID ENTTYLAIIA
60 70 80 90 100
FSQYVQEASF DEVETLVKVM LDYRDRCWAD NTLPECSKTA NDAIQDMLCD
110 120 130 140 150
MEGLPQKHNF SHCCGKAGFP RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YKNNSESFLH LYMYEVARRN PFVFAPVLLA VAAWFEEAAT TCCEQQQKAT
210 220 230 240 250
CFQAKAAPIT QYLKASSSYQ RNVCGALIKF GPKVLNSINV AVFSKKFPKI
260 270 280 290 300
GFKDLTTLLE DVSSMYEGCC EGDVVHCIRS QSQVVNHICS KQDSISSKIK
310 320 330 340 350
VCCEKKTLER EACIINANKD DRPEGLSLRE AKFTESENVC QERDSDPDKF
360 370 380 390 400
FAEFIYEYSR RHPDLSTPEL LRITKVYMDF LEDCCSRENP AGCYRHVEDK
410 420 430 440 450
FNETTQRSLA MVQQECKQFQ ELGKDTLQRH FLVKFTKAAP QLPMEELVSL
460 470 480 490 500
SKEMVAALTT CCTLSDEFAC VDNLADLVLG ELCGVNTNRT INPAVDHCCK
510 520 530 540 550
TDFAFRRHCF EHLKADTTYE LPSVSALVSA LHTDWCQPRK EDLQNKKHRF
560 570 580 590 600
LVNLVKWMPG ITDEEWLCLF TKFTAAREEC SEVQEPESCF SPESSKTGDE

SQATEKQR
Length:608
Mass (Da):69,379
Last modified:February 20, 2007 - v2
Checksum:i98B31E6D96E73F12
GO
Isoform 2 (identifier: O89020-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     430-430: H → Q
     431-608: Missing.

Show »
Length:430
Mass (Da):49,275
Checksum:i1CF6CB19386A4976
GO
Isoform 3 (identifier: O89020-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     606-608: KQR → NKITDQ

Note: No experimental confirmation available.
Show »
Length:611
Mass (Da):69,666
Checksum:iF0EE392CF6F91D96
GO

Sequence cautioni

The sequence AAH26681 differs from that shown. Contaminating sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44T → A in CAA09471 (Ref. 1) Curated1
Sequence conflicti44T → A in AAH26681 (PubMed:15489334).Curated1
Sequence conflicti154N → K in CAA09471 (Ref. 1) Curated1
Sequence conflicti280S → N in AAI00598 (PubMed:15489334).Curated1
Sequence conflicti285V → E in CAA09471 (Ref. 1) Curated1
Sequence conflicti295I → V in AAI00598 (PubMed:15489334).Curated1
Sequence conflicti307T → I in AAI00598 (PubMed:15489334).Curated1
Sequence conflicti369E → R in CAA09471 (Ref. 1) Curated1
Sequence conflicti417K → N in CAA09471 (Ref. 1) Curated1
Sequence conflicti520E → A in CAA09471 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023387430H → Q in isoform 2. 2 Publications1
Alternative sequenceiVSP_023388431 – 608Missing in isoform 2. 2 PublicationsAdd BLAST178
Alternative sequenceiVSP_023389606 – 608KQR → NKITDQ in isoform 3. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011080 mRNA Translation: CAA09471.1
AK143987 mRNA Translation: BAE25647.1
BC026681 mRNA Translation: AAH26681.1 Sequence problems.
BC100597 mRNA Translation: AAI00598.1
CCDSiCCDS39141.1 [O89020-1]
RefSeqiNP_660128.2, NM_145146.2 [O89020-1]
UniGeneiMm.348786

Genome annotation databases

EnsembliENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369 [O89020-1]
ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369 [O89020-2]
GeneIDi280662
KEGGimmu:280662
UCSCiuc008ybb.1 mouse [O89020-2]
uc008ybc.1 mouse [O89020-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011080 mRNA Translation: CAA09471.1
AK143987 mRNA Translation: BAE25647.1
BC026681 mRNA Translation: AAH26681.1 Sequence problems.
BC100597 mRNA Translation: AAI00598.1
CCDSiCCDS39141.1 [O89020-1]
RefSeqiNP_660128.2, NM_145146.2 [O89020-1]
UniGeneiMm.348786

3D structure databases

ProteinModelPortaliO89020
SMRiO89020
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO89020, 1 interactor
MINTiO89020
STRINGi10090.ENSMUSP00000108804

PTM databases

iPTMnetiO89020
PhosphoSitePlusiO89020

Proteomic databases

MaxQBiO89020
PaxDbiO89020
PeptideAtlasiO89020
PRIDEiO89020

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369 [O89020-1]
ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369 [O89020-2]
GeneIDi280662
KEGGimmu:280662
UCSCiuc008ybb.1 mouse [O89020-2]
uc008ybc.1 mouse [O89020-1]

Organism-specific databases

CTDi173
MGIiMGI:2429409 Afm

Phylogenomic databases

eggNOGiENOG410IIRZ Eukaryota
ENOG410Z40H LUCA
GeneTreeiENSGT00390000000113
HOGENOMiHOG000293137
HOVERGENiHBG004207
InParanoidiO89020
OMAiFACVDNL
OrthoDBiEOG091G0F5F
PhylomeDBiO89020
TreeFamiTF335561

Miscellaneous databases

ChiTaRSiAfm mouse
PROiPR:O89020
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029369 Expressed in 78 organ(s), highest expression level in liver
CleanExiMM_AFM
GenevisibleiO89020 MM

Family and domain databases

CDDicd00015 ALBUMIN, 3 hits
InterProiView protein in InterPro
IPR000264 ALB/AFP/VDB
IPR020858 Serum_albumin-like
IPR021177 Serum_albumin/AFP/Afamin
IPR020857 Serum_albumin_CS
IPR014760 Serum_albumin_N
PANTHERiPTHR11385 PTHR11385, 1 hit
PfamiView protein in Pfam
PF00273 Serum_albumin, 3 hits
PIRSFiPIRSF002520 Serum_albumin_subgroup, 1 hit
PRINTSiPR00803 AFETOPROTEIN
PR00802 SERUMALBUMIN
SMARTiView protein in SMART
SM00103 ALBUMIN, 3 hits
SUPFAMiSSF48552 SSF48552, 3 hits
PROSITEiView protein in PROSITE
PS00212 ALBUMIN_1, 2 hits
PS51438 ALBUMIN_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiAFAM_MOUSE
AccessioniPrimary (citable) accession number: O89020
Secondary accession number(s): Q3UNV0, Q497E6, Q8R0J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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