UniProtKB - O89020 (AFAM_MOUSE)
Protein
Afamin
Gene
Afm
Organism
Mus musculus (Mouse)
Status
Functioni
Functions as carrier for hydrophobic molecules in body fluids. Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.By similarity
GO - Molecular functioni
- fatty acid binding Source: GO_Central
- vitamin E binding Source: UniProtKB
- zinc ion binding Source: GO_Central
GO - Biological processi
- protein stabilization Source: UniProtKB
- protein transport within extracellular region Source: UniProtKB
- vitamin transport Source: UniProtKB
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: AfaminAlternative name(s): Alpha-albumin Short name: Alpha-Alb |
Gene namesi | Name:Afm |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2429409, Afm |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | By similarityAdd BLAST | 21 | |
ChainiPRO_0000001107 | 22 – 608 | AfaminAdd BLAST | 587 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 33 | N-linked (GlcNAc...) asparagine3 Publications | 1 | |
Disulfide bondi | 77 ↔ 86 | PROSITE-ProRule annotation | ||
Disulfide bondi | 99 ↔ 114 | PROSITE-ProRule annotation | ||
Glycosylationi | 109 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 113 ↔ 124 | PROSITE-ProRule annotation | ||
Disulfide bondi | 148 ↔ 193 | PROSITE-ProRule annotation | ||
Glycosylationi | 153 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 192 ↔ 201 | PROSITE-ProRule annotation | ||
Disulfide bondi | 224 ↔ 270 | PROSITE-ProRule annotation | ||
Disulfide bondi | 269 ↔ 277 | PROSITE-ProRule annotation | ||
Disulfide bondi | 289 ↔ 303 | PROSITE-ProRule annotation | ||
Disulfide bondi | 302 ↔ 313 | PROSITE-ProRule annotation | ||
Disulfide bondi | 340 ↔ 385 | PROSITE-ProRule annotation | ||
Disulfide bondi | 384 ↔ 393 | PROSITE-ProRule annotation | ||
Glycosylationi | 402 | N-linked (GlcNAc...) asparagine3 Publications | 1 | |
Disulfide bondi | 416 ↔ 462 | PROSITE-ProRule annotation | ||
Disulfide bondi | 461 ↔ 470 | PROSITE-ProRule annotation | ||
Disulfide bondi | 483 ↔ 499 | PROSITE-ProRule annotation | ||
Glycosylationi | 488 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 498 ↔ 509 | PROSITE-ProRule annotation | ||
Disulfide bondi | 580 ↔ 589 | PROSITE-ProRule annotation |
Post-translational modificationi
N-glycosylated; more than 90% of the glycans are sialylated.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
CPTACi | non-CPTAC-3328 |
MaxQBi | O89020 |
PaxDbi | O89020 |
PeptideAtlasi | O89020 |
PRIDEi | O89020 |
PTM databases
GlyGeni | O89020, 5 sites |
iPTMneti | O89020 |
PhosphoSitePlusi | O89020 |
Expressioni
Tissue specificityi
Detected in brain, especially on brain capillaries (at protein level). Expressed in isolated brain capillaries.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000029369, Expressed in liver and 88 other tissues |
Genevisiblei | O89020, MM |
Interactioni
Subunit structurei
Forms a 1:1 complex with Wnt family members; interacts with WNT1, WNT2B, WNT3, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (By similarity).
Interacts with WNT3A (PubMed:26902720).
By similarity1 PublicationProtein-protein interaction databases
IntActi | O89020, 1 interactor |
MINTi | O89020 |
STRINGi | 10090.ENSMUSP00000108804 |
Miscellaneous databases
RNActi | O89020, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 210 | Albumin 1PROSITE-ProRule annotationAdd BLAST | 189 | |
Domaini | 211 – 403 | Albumin 2PROSITE-ProRule annotationAdd BLAST | 193 | |
Domaini | 404 – 599 | Albumin 3PROSITE-ProRule annotationAdd BLAST | 196 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 215 – 319 | Binding pocket for hydrophobic ligandsBy similarityAdd BLAST | 105 |
Domaini
The second albumin domain forms a deep binding pocket that contains palmitoleic acid (in vitro). Palmitoleic acid is most likely not the physiological ligand. Instead, this pocket may accomodate the covalently bound lipid moiety of Wnt family members.By similarity
Sequence similaritiesi
Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502R7EA, Eukaryota |
GeneTreei | ENSGT00390000000113 |
HOGENOMi | CLU_030161_2_0_1 |
InParanoidi | O89020 |
OMAi | RAIPVTQ |
OrthoDBi | 906547at2759 |
PhylomeDBi | O89020 |
TreeFami | TF335561 |
Family and domain databases
CDDi | cd00015, ALBUMIN, 3 hits |
InterProi | View protein in InterPro IPR000264, ALB/AFP/VDB IPR020858, Serum_albumin-like IPR021177, Serum_albumin/AFP/Afamin IPR020857, Serum_albumin_CS IPR014760, Serum_albumin_N |
PANTHERi | PTHR11385, PTHR11385, 1 hit |
Pfami | View protein in Pfam PF00273, Serum_albumin, 3 hits |
PIRSFi | PIRSF002520, Serum_albumin_subgroup, 1 hit |
PRINTSi | PR00803, AFETOPROTEIN PR00802, SERUMALBUMIN |
SMARTi | View protein in SMART SM00103, ALBUMIN, 3 hits |
SUPFAMi | SSF48552, SSF48552, 3 hits |
PROSITEi | View protein in PROSITE PS00212, ALBUMIN_1, 2 hits PS51438, ALBUMIN_2, 3 hits |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: O89020-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRHLKLTGFI FFLLPLTESL ALPTKPQDVD HFNATQKFID ENTTYLAIIA
60 70 80 90 100
FSQYVQEASF DEVETLVKVM LDYRDRCWAD NTLPECSKTA NDAIQDMLCD
110 120 130 140 150
MEGLPQKHNF SHCCGKAGFP RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YKNNSESFLH LYMYEVARRN PFVFAPVLLA VAAWFEEAAT TCCEQQQKAT
210 220 230 240 250
CFQAKAAPIT QYLKASSSYQ RNVCGALIKF GPKVLNSINV AVFSKKFPKI
260 270 280 290 300
GFKDLTTLLE DVSSMYEGCC EGDVVHCIRS QSQVVNHICS KQDSISSKIK
310 320 330 340 350
VCCEKKTLER EACIINANKD DRPEGLSLRE AKFTESENVC QERDSDPDKF
360 370 380 390 400
FAEFIYEYSR RHPDLSTPEL LRITKVYMDF LEDCCSRENP AGCYRHVEDK
410 420 430 440 450
FNETTQRSLA MVQQECKQFQ ELGKDTLQRH FLVKFTKAAP QLPMEELVSL
460 470 480 490 500
SKEMVAALTT CCTLSDEFAC VDNLADLVLG ELCGVNTNRT INPAVDHCCK
510 520 530 540 550
TDFAFRRHCF EHLKADTTYE LPSVSALVSA LHTDWCQPRK EDLQNKKHRF
560 570 580 590 600
LVNLVKWMPG ITDEEWLCLF TKFTAAREEC SEVQEPESCF SPESSKTGDE
SQATEKQR
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 44 | T → A in CAA09471 (Ref. 1) Curated | 1 | |
Sequence conflicti | 44 | T → A in AAH26681 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 154 | N → K in CAA09471 (Ref. 1) Curated | 1 | |
Sequence conflicti | 280 | S → N in AAI00598 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 285 | V → E in CAA09471 (Ref. 1) Curated | 1 | |
Sequence conflicti | 295 | I → V in AAI00598 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 307 | T → I in AAI00598 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 369 | E → R in CAA09471 (Ref. 1) Curated | 1 | |
Sequence conflicti | 417 | K → N in CAA09471 (Ref. 1) Curated | 1 | |
Sequence conflicti | 520 | E → A in CAA09471 (Ref. 1) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023387 | 430 | H → Q in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_023388 | 431 – 608 | Missing in isoform 2. 2 PublicationsAdd BLAST | 178 | |
Alternative sequenceiVSP_023389 | 606 – 608 | KQR → NKITDQ in isoform 3. 1 Publication | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ011080 mRNA Translation: CAA09471.1 AK143987 mRNA Translation: BAE25647.1 BC026681 mRNA Translation: AAH26681.1 Sequence problems. BC100597 mRNA Translation: AAI00598.1 |
CCDSi | CCDS39141.1 [O89020-1] |
RefSeqi | NP_660128.2, NM_145146.2 [O89020-1] |
Genome annotation databases
Ensembli | ENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369 [O89020-1] ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369 [O89020-2] |
GeneIDi | 280662 |
KEGGi | mmu:280662 |
UCSCi | uc008ybb.1, mouse [O89020-2] uc008ybc.1, mouse [O89020-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ011080 mRNA Translation: CAA09471.1 AK143987 mRNA Translation: BAE25647.1 BC026681 mRNA Translation: AAH26681.1 Sequence problems. BC100597 mRNA Translation: AAI00598.1 |
CCDSi | CCDS39141.1 [O89020-1] |
RefSeqi | NP_660128.2, NM_145146.2 [O89020-1] |
3D structure databases
SMRi | O89020 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O89020, 1 interactor |
MINTi | O89020 |
STRINGi | 10090.ENSMUSP00000108804 |
PTM databases
GlyGeni | O89020, 5 sites |
iPTMneti | O89020 |
PhosphoSitePlusi | O89020 |
Proteomic databases
CPTACi | non-CPTAC-3328 |
MaxQBi | O89020 |
PaxDbi | O89020 |
PeptideAtlasi | O89020 |
PRIDEi | O89020 |
Protocols and materials databases
Antibodypediai | 1356, 232 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000113179; ENSMUSP00000108804; ENSMUSG00000029369 [O89020-1] ENSMUST00000128740; ENSMUSP00000117180; ENSMUSG00000029369 [O89020-2] |
GeneIDi | 280662 |
KEGGi | mmu:280662 |
UCSCi | uc008ybb.1, mouse [O89020-2] uc008ybc.1, mouse [O89020-1] |
Organism-specific databases
CTDi | 173 |
MGIi | MGI:2429409, Afm |
Phylogenomic databases
eggNOGi | ENOG502R7EA, Eukaryota |
GeneTreei | ENSGT00390000000113 |
HOGENOMi | CLU_030161_2_0_1 |
InParanoidi | O89020 |
OMAi | RAIPVTQ |
OrthoDBi | 906547at2759 |
PhylomeDBi | O89020 |
TreeFami | TF335561 |
Miscellaneous databases
BioGRID-ORCSi | 280662, 1 hit in 17 CRISPR screens |
ChiTaRSi | Afm, mouse |
PROi | PR:O89020 |
RNActi | O89020, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029369, Expressed in liver and 88 other tissues |
Genevisiblei | O89020, MM |
Family and domain databases
CDDi | cd00015, ALBUMIN, 3 hits |
InterProi | View protein in InterPro IPR000264, ALB/AFP/VDB IPR020858, Serum_albumin-like IPR021177, Serum_albumin/AFP/Afamin IPR020857, Serum_albumin_CS IPR014760, Serum_albumin_N |
PANTHERi | PTHR11385, PTHR11385, 1 hit |
Pfami | View protein in Pfam PF00273, Serum_albumin, 3 hits |
PIRSFi | PIRSF002520, Serum_albumin_subgroup, 1 hit |
PRINTSi | PR00803, AFETOPROTEIN PR00802, SERUMALBUMIN |
SMARTi | View protein in SMART SM00103, ALBUMIN, 3 hits |
SUPFAMi | SSF48552, SSF48552, 3 hits |
PROSITEi | View protein in PROSITE PS00212, ALBUMIN_1, 2 hits PS51438, ALBUMIN_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AFAM_MOUSE | |
Accessioni | O89020Primary (citable) accession number: O89020 Secondary accession number(s): Q3UNV0, Q497E6, Q8R0J9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | February 20, 2007 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families