Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Legumain

Gene

Lgmn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation.4 Publications

Catalytic activityi

Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.3 Publications

Activity regulationi

Inhibited by cystatin-C.1 Publication

pH dependencei

Optimum pH is 6.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1501 Publication1
Active sitei191Nucleophile1 Publication1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: UniProtKB
  • peptidase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.34 3474
ReactomeiR-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-196791 Vitamin D (calciferol) metabolism
R-MMU-2132295 MHC class II antigen presentation

Protein family/group databases

MEROPSiC13.004

Names & Taxonomyi

Protein namesi
Recommended name:
Legumain (EC:3.4.22.34)
Alternative name(s):
Asparaginyl endopeptidase
Protease, cysteine 1
Gene namesi
Name:Lgmn
Synonyms:Prsc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1330838 Lgmn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Disruption phenotypei

Young mice initially display no obvious phenotype, but fail to gain weight normally. Mutant mice display pale kidneys with abnormal proliferation of proximal tubule cells, proximal tubule hyperplasia and develop kidney interstitium fibrosis. After 6 months, mutant mice display a decreased glomerular filtration rate, increased plasma creatinine levels and proteinuria. Glomerular lysosomes do not show a generalized defect in protein catabolism. Instead they show defects in the degradation of a set of target proteins, including EGFR.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44N → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi46R → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi47H → A: 54% Loss of activity. 1 Publication1
Mutagenesisi52C → S: No loss of activity. 1 Publication1
Mutagenesisi150H → A: Complete loss of activity. Abolishes autocatalytic processing. 2 Publications1
Mutagenesisi189E → A: Abolishes enzyme activity. 1 Publication1
Mutagenesisi191C → A or S: Abolishes enzyme activity. 2 Publications1
Mutagenesisi233D → A: Abolishes enzyme activity. Abolishes autocatalytic processing. 1 Publication1
Mutagenesisi311D → A: Nearly abolishes enzyme activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1949492

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17By similarityAdd BLAST17
ChainiPRO_000002650418 – 325LegumainAdd BLAST308
PropeptideiPRO_0000026505326 – 435Add BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi93N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi169N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi265N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi274N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi380 ↔ 4141 Publication
Disulfide bondi392 ↔ 4311 Publication

Post-translational modificationi

Glycosylated.1 Publication
Activated by autocatalytic processing at pH 4.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei325 – 326Cleavage; by autolysis2

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiO89017
MaxQBiO89017
PaxDbiO89017
PeptideAtlasiO89017
PRIDEiO89017

PTM databases

iPTMnetiO89017
PhosphoSitePlusiO89017
SwissPalmiO89017

Miscellaneous databases

PMAP-CutDBiO89017

Expressioni

Tissue specificityi

Detected in kidney proximal tubules (at protein level). Ubiquitous. Particularly abundant in kidney and placenta.2 Publications

Gene expression databases

BgeeiENSMUSG00000021190 Expressed in 296 organ(s), highest expression level in ileum
CleanExiMM_LGMN
ExpressionAtlasiO89017 baseline and differential
GenevisibleiO89017 MM

Interactioni

Subunit structurei

May interact with integrins (By similarity). Monomer after autocatalytic processing. Homodimer before autocatalytic removal of the propeptide.By similarity1 Publication

Protein-protein interaction databases

BioGridi202402, 1 interactor
IntActiO89017, 2 interactors
MINTiO89017
STRINGi10090.ENSMUSP00000021607

Chemistry databases

BindingDBiO89017

Structurei

Secondary structure

1435
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO89017
SMRiO89017
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

In the zymogen form, the uncleaved propeptide blocks access to the active site.By similarity

Sequence similaritiesi

Belongs to the peptidase C13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1348 Eukaryota
COG5206 LUCA
GeneTreeiENSGT00530000063391
HOGENOMiHOG000236335
HOVERGENiHBG031304
InParanoidiO89017
KOiK01369
OMAiGPKDHVF
OrthoDBiEOG091G08C5
PhylomeDBiO89017
TreeFamiTF313403

Family and domain databases

InterProiView protein in InterPro
IPR001096 Peptidase_C13
PANTHERiPTHR12000 PTHR12000, 1 hit
PfamiView protein in Pfam
PF01650 Peptidase_C13, 1 hit
PIRSFiPIRSF019663 Legumain, 1 hit
PRINTSiPR00776 HEMOGLOBNASE

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O89017-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTWRVAVLLS LVLGAGAVPV GVDDPEDGGK HWVVIVAGSN GWYNYRHQAD
60 70 80 90 100
ACHAYQIIHR NGIPDEQIIV MMYDDIANSE ENPTPGVVIN RPNGTDVYKG
110 120 130 140 150
VLKDYTGEDV TPENFLAVLR GDAEAVKGKG SGKVLKSGPR DHVFIYFTDH
160 170 180 190 200
GATGILVFPN DDLHVKDLNK TIRYMYEHKM YQKMVFYIEA CESGSMMNHL
210 220 230 240 250
PDDINVYATT AANPKESSYA CYYDEERGTY LGDWYSVNWM EDSDVEDLTK
260 270 280 290 300
ETLHKQYHLV KSHTNTSHVM QYGNKSISTM KVMQFQGMKH RASSPISLPP
310 320 330 340 350
VTHLDLTPSP DVPLTILKRK LLRTNDVKES QNLIGQIQQF LDARHVIEKS
360 370 380 390 400
VHKIVSLLAG FGETAERHLS ERTMLTAHDC YQEAVTHFRT HCFNWHSVTY
410 420 430
EHALRYLYVL ANLCEAPYPI DRIEMAMDKV CLSHY
Length:435
Mass (Da):49,373
Last modified:November 1, 1998 - v1
Checksum:iF956B9E10098013D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000990 mRNA Translation: CAA04439.1
AF044266 mRNA Translation: AAF21659.1
CCDSiCCDS26119.1
RefSeqiNP_035305.1, NM_011175.3
UniGeneiMm.17185

Genome annotation databases

EnsembliENSMUST00000021607; ENSMUSP00000021607; ENSMUSG00000021190
ENSMUST00000110020; ENSMUSP00000105647; ENSMUSG00000021190
GeneIDi19141
KEGGimmu:19141
UCSCiuc007oue.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000990 mRNA Translation: CAA04439.1
AF044266 mRNA Translation: AAF21659.1
CCDSiCCDS26119.1
RefSeqiNP_035305.1, NM_011175.3
UniGeneiMm.17185

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NOJX-ray2.80A27-289[»]
4NOKX-ray2.50A1-435[»]
4NOLX-ray2.70A/B1-435[»]
4NOMX-ray2.01A1-435[»]
ProteinModelPortaliO89017
SMRiO89017
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202402, 1 interactor
IntActiO89017, 2 interactors
MINTiO89017
STRINGi10090.ENSMUSP00000021607

Chemistry databases

BindingDBiO89017
ChEMBLiCHEMBL1949492

Protein family/group databases

MEROPSiC13.004

PTM databases

iPTMnetiO89017
PhosphoSitePlusiO89017
SwissPalmiO89017

Proteomic databases

EPDiO89017
MaxQBiO89017
PaxDbiO89017
PeptideAtlasiO89017
PRIDEiO89017

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021607; ENSMUSP00000021607; ENSMUSG00000021190
ENSMUST00000110020; ENSMUSP00000105647; ENSMUSG00000021190
GeneIDi19141
KEGGimmu:19141
UCSCiuc007oue.1 mouse

Organism-specific databases

CTDi5641
MGIiMGI:1330838 Lgmn

Phylogenomic databases

eggNOGiKOG1348 Eukaryota
COG5206 LUCA
GeneTreeiENSGT00530000063391
HOGENOMiHOG000236335
HOVERGENiHBG031304
InParanoidiO89017
KOiK01369
OMAiGPKDHVF
OrthoDBiEOG091G08C5
PhylomeDBiO89017
TreeFamiTF313403

Enzyme and pathway databases

BRENDAi3.4.22.34 3474
ReactomeiR-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-196791 Vitamin D (calciferol) metabolism
R-MMU-2132295 MHC class II antigen presentation

Miscellaneous databases

ChiTaRSiLgmn mouse
PMAP-CutDBiO89017
PROiPR:O89017
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021190 Expressed in 296 organ(s), highest expression level in ileum
CleanExiMM_LGMN
ExpressionAtlasiO89017 baseline and differential
GenevisibleiO89017 MM

Family and domain databases

InterProiView protein in InterPro
IPR001096 Peptidase_C13
PANTHERiPTHR12000 PTHR12000, 1 hit
PfamiView protein in Pfam
PF01650 Peptidase_C13, 1 hit
PIRSFiPIRSF019663 Legumain, 1 hit
PRINTSiPR00776 HEMOGLOBNASE
ProtoNetiSearch...

Entry informationi

Entry nameiLGMN_MOUSE
AccessioniPrimary (citable) accession number: O89017
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 24, 2002
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again