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Protein

Alpha-actinin-3

Gene

Actn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi772 – 7831; possibly ancestralAdd BLAST12
Calcium bindingi808 – 8192; possibly ancestralAdd BLAST12

GO - Molecular functioni

  • actin filament binding Source: MGI
  • calcium ion binding Source: InterPro
  • protein binding, bridging Source: MGI
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActin-binding
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-390522 Striated Muscle Contraction

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-3
Alternative name(s):
Alpha-actinin skeletal muscle isoform 3
F-actin cross-linking protein
Gene namesi
Name:Actn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:99678 Actn3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000734391 – 900Alpha-actinin-3Add BLAST900

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO88990
MaxQBiO88990
PaxDbiO88990
PeptideAtlasiO88990
PRIDEiO88990

PTM databases

iPTMnetiO88990
PhosphoSitePlusiO88990

Expressioni

Gene expression databases

BgeeiENSMUSG00000006457 Expressed in 157 organ(s), highest expression level in vastus lateralis
CleanExiMM_ACTN3
GenevisibleiO88990 MM

Interactioni

Subunit structurei

Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197951, 3 interactors
IntActiO88990, 6 interactors
MINTiO88990
STRINGi10090.ENSMUSP00000006626

Structurei

3D structure databases

ProteinModelPortaliO88990
SMRiO88990
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 148Calponin-homology (CH) 1PROSITE-ProRule annotationAdd BLAST105
Domaini157 – 263Calponin-homology (CH) 2PROSITE-ProRule annotationAdd BLAST107
Repeati287 – 397Spectrin 1Add BLAST111
Repeati407 – 512Spectrin 2Add BLAST106
Repeati522 – 633Spectrin 3Add BLAST112
Repeati643 – 746Spectrin 4Add BLAST104
Domaini759 – 794EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini795 – 830EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 260Actin-bindingAdd BLAST260

Sequence similaritiesi

Belongs to the alpha-actinin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0035 Eukaryota
COG5069 LUCA
GeneTreeiENSGT00760000118813
HOGENOMiHOG000263418
HOVERGENiHBG050453
InParanoidiO88990
KOiK21073
OMAiQTMRAMQ
OrthoDBiEOG091G020R
PhylomeDBiO88990
TreeFamiTF352676

Family and domain databases

CDDicd00014 CH, 2 hits
cd00051 EFh, 1 hit
Gene3Di1.10.418.10, 2 hits
InterProiView protein in InterPro
IPR001589 Actinin_actin-bd_CS
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR011992 EF-hand-dom_pair
IPR014837 EF-hand_Ca_insen
IPR002048 EF_hand_dom
IPR018159 Spectrin/alpha-actinin
IPR002017 Spectrin_repeat
PfamiView protein in Pfam
PF00307 CH, 2 hits
PF08726 EFhand_Ca_insen, 1 hit
PF00435 Spectrin, 4 hits
SMARTiView protein in SMART
SM00033 CH, 2 hits
SM00054 EFh, 2 hits
SM00150 SPEC, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS00019 ACTININ_1, 1 hit
PS00020 ACTININ_2, 1 hit
PS50021 CH, 2 hits
PS50222 EF_HAND_2, 2 hits

Sequencei

Sequence statusi: Complete.

O88990-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMMVMQPEGL GAGEGPFSGG GGGEYMEQEE DWDRDLLLDP AWEKQQRKTF
60 70 80 90 100
TAWCNSHLRK AGTQIENIEE DFRNGLKLML LLEVISGERL PRPDKGKMRF
110 120 130 140 150
HKIANVNKAL DFIASKGVKL VSIGAEEIVD GNLKMTLGMI WTIILRFAIQ
160 170 180 190 200
DISVEETSAK EGLLLWCQRK TAPYRNVNVQ NFHTSWKDGL ALCALIHRHR
210 220 230 240 250
PDLIDYAKLR KDDPIGNLNT AFEVAEKYLD IPKMLDAEDI VNTPKPDEKA
260 270 280 290 300
IMTYVSCFYH AFAGAEQAET AANRICKVLA VNQENEKLME EYEKLASELL
310 320 330 340 350
EWIRRTVPWL ENRVGEPSMS AMQRKLEDFR DYRRLHKPPR VQEKCQLEIN
360 370 380 390 400
FNTLQTKLRL SHRPAFMPSE GKLVSDIANA WRGLEQVEKG YEDWLLSEIR
410 420 430 440 450
RLQRLQHLAE KFQQKASLHE AWTRGKEEML NQHDYESASL QEVRALLRRH
460 470 480 490 500
EAFESDLAAH QDRVEHIAAL AQELNELDYH EAASVNSRCQ AICDQWDNLG
510 520 530 540 550
TLTQKRRDAL ERMEKLLETI DQLQLEFARR AAPFNNWLDG AIEDLQDVWL
560 570 580 590 600
VHSVEETQSL LTAHEQFKAT LPEADRERGA ILGIQGEIQK ICQTYGLRPK
610 620 630 640 650
SGNPYITLSS QDINNKWDTV RKLVPSRDQT LQEELARQQV NERLRRQFAA
660 670 680 690 700
QANAIGPWIQ GKVEEVGRLA AGLAGSLEEQ MAGLRQQEQN IINYKSNIDR
710 720 730 740 750
LEGDHQLLQE SLVFDNKHTV YSMEHIRVGW EQLLTSIART INEVENQVLT
760 770 780 790 800
RDAKGLSQEQ LNEFRASFNH FDRKRNGMME PDDFRACLIS MGYDLGEVEF
810 820 830 840 850
ARIMTMVDPN AAGVVTFQAF IDFMTRETAE TDTAEQVVAS FKILAGDKNY
860 870 880 890 900
ITPEELRREL PAEQAEYCIR RMAPYKGSGA PSGALDYVAF SSALYGESDL
Length:900
Mass (Da):103,043
Last modified:November 1, 1998 - v1
Checksum:i7F795002CB2B7F8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093775 mRNA Translation: AAC62512.1
BC111890 mRNA Translation: AAI11891.1
CCDSiCCDS29441.1
RefSeqiNP_038484.1, NM_013456.2
UniGeneiMm.5316

Genome annotation databases

EnsembliENSMUST00000006626; ENSMUSP00000006626; ENSMUSG00000006457
GeneIDi11474
KEGGimmu:11474
UCSCiuc008gbf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093775 mRNA Translation: AAC62512.1
BC111890 mRNA Translation: AAI11891.1
CCDSiCCDS29441.1
RefSeqiNP_038484.1, NM_013456.2
UniGeneiMm.5316

3D structure databases

ProteinModelPortaliO88990
SMRiO88990
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197951, 3 interactors
IntActiO88990, 6 interactors
MINTiO88990
STRINGi10090.ENSMUSP00000006626

PTM databases

iPTMnetiO88990
PhosphoSitePlusiO88990

Proteomic databases

EPDiO88990
MaxQBiO88990
PaxDbiO88990
PeptideAtlasiO88990
PRIDEiO88990

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006626; ENSMUSP00000006626; ENSMUSG00000006457
GeneIDi11474
KEGGimmu:11474
UCSCiuc008gbf.2 mouse

Organism-specific databases

CTDi89
MGIiMGI:99678 Actn3

Phylogenomic databases

eggNOGiKOG0035 Eukaryota
COG5069 LUCA
GeneTreeiENSGT00760000118813
HOGENOMiHOG000263418
HOVERGENiHBG050453
InParanoidiO88990
KOiK21073
OMAiQTMRAMQ
OrthoDBiEOG091G020R
PhylomeDBiO88990
TreeFamiTF352676

Enzyme and pathway databases

ReactomeiR-MMU-390522 Striated Muscle Contraction

Miscellaneous databases

ChiTaRSiActn3 mouse
PROiPR:O88990
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006457 Expressed in 157 organ(s), highest expression level in vastus lateralis
CleanExiMM_ACTN3
GenevisibleiO88990 MM

Family and domain databases

CDDicd00014 CH, 2 hits
cd00051 EFh, 1 hit
Gene3Di1.10.418.10, 2 hits
InterProiView protein in InterPro
IPR001589 Actinin_actin-bd_CS
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR011992 EF-hand-dom_pair
IPR014837 EF-hand_Ca_insen
IPR002048 EF_hand_dom
IPR018159 Spectrin/alpha-actinin
IPR002017 Spectrin_repeat
PfamiView protein in Pfam
PF00307 CH, 2 hits
PF08726 EFhand_Ca_insen, 1 hit
PF00435 Spectrin, 4 hits
SMARTiView protein in SMART
SM00033 CH, 2 hits
SM00054 EFh, 2 hits
SM00150 SPEC, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS00019 ACTININ_1, 1 hit
PS00020 ACTININ_2, 1 hit
PS50021 CH, 2 hits
PS50222 EF_HAND_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiACTN3_MOUSE
AccessioniPrimary (citable) accession number: O88990
Secondary accession number(s): Q14DS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: September 12, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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