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Protein

Malate dehydrogenase, cytoplasmic

Gene

Mdh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei105NADBy similarity1
Binding sitei112NADBy similarity1
Binding sitei131SubstrateBy similarity1
Binding sitei162SubstrateBy similarity1
Active sitei187Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 17NADBy similarity7
Nucleotide bindingi129 – 131NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

ReactomeiR-RNO-70263 Gluconeogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:Mdh1
Synonyms:Mdh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi3072 Mdh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002267372 – 334Malate dehydrogenase, cytoplasmicAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei110N6-succinyllysineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Modified residuei121N6-acetyllysineBy similarity1
Modified residuei214N6-succinyllysineBy similarity1
Modified residuei217PhosphoserineCombined sources1
Modified residuei230Omega-N-methylarginineBy similarity1
Modified residuei241PhosphoserineCombined sources1
Modified residuei298N6-acetyllysine; alternateBy similarity1
Modified residuei298N6-succinyllysine; alternateBy similarity1
Modified residuei309PhosphoserineBy similarity1
Modified residuei318N6-succinyllysineBy similarity1
Modified residuei332PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.By similarity
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88989
PRIDEiO88989

2D gel databases

World-2DPAGEi0004:O88989

PTM databases

iPTMnetiO88989
PhosphoSitePlusiO88989
SwissPalmiO88989

Expressioni

Gene expression databases

BgeeiENSRNOG00000008103 Expressed in 10 organ(s), highest expression level in heart
GenevisibleiO88989 RN

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi246700, 4 interactors
CORUMiO88989
IntActiO88989, 2 interactors
STRINGi10116.ENSRNOP00000011429

Structurei

3D structure databases

ProteinModelPortaliO88989
SMRiO88989
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiKOG1496 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00530000063410
HOGENOMiHOG000220953
HOVERGENiHBG006340
InParanoidiO88989
KOiK00025
OMAiDPMTAFK
OrthoDBiEOG091G0BLA
PhylomeDBiO88989
TreeFamiTF105826

Family and domain databases

CDDicd01336 MDH_cytoplasmic_cytosolic, 1 hit
Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01517 Malate_dehydrog_2, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR011274 Malate_DH_NAD-dep_euk
IPR010945 Malate_DH_type2
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23382 PTHR23382, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01759 MalateDH-SF1, 1 hit
TIGR01758 MDH_euk_cyt, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88989-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL
60 70 80 90 100
DGVLMELQDC ALPLLQDVIA TDKEEVAFKD LDVAVLVGSM PRREGMERKD
110 120 130 140 150
LLKANVKIFK SQGAALEKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE
160 170 180 190 200
NFSCLTRLDH NRAKSQIALK LGVTADDVKN VIIWGNHSST QYPDVNHAKV
210 220 230 240 250
KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI
260 270 280 290 300
SDHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV
310 320 330
EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA
Length:334
Mass (Da):36,483
Last modified:January 23, 2007 - v3
Checksum:i8F6778722A607B3C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti251S → A in AAC26799 (Ref. 4) Curated1
Sequence conflicti276N → D in AAH59124 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093773 mRNA Translation: AAC64180.1
BC059124 mRNA Translation: AAH59124.1
AF075574 mRNA Translation: AAC26799.1
RefSeqiNP_150238.1, NM_033235.2
UniGeneiRn.13492

Genome annotation databases

EnsembliENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103
GeneIDi24551
KEGGirno:24551
UCSCiRGD:3072 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093773 mRNA Translation: AAC64180.1
BC059124 mRNA Translation: AAH59124.1
AF075574 mRNA Translation: AAC26799.1
RefSeqiNP_150238.1, NM_033235.2
UniGeneiRn.13492

3D structure databases

ProteinModelPortaliO88989
SMRiO88989
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246700, 4 interactors
CORUMiO88989
IntActiO88989, 2 interactors
STRINGi10116.ENSRNOP00000011429

PTM databases

iPTMnetiO88989
PhosphoSitePlusiO88989
SwissPalmiO88989

2D gel databases

World-2DPAGEi0004:O88989

Proteomic databases

PaxDbiO88989
PRIDEiO88989

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103
GeneIDi24551
KEGGirno:24551
UCSCiRGD:3072 rat

Organism-specific databases

CTDi4190
RGDi3072 Mdh1

Phylogenomic databases

eggNOGiKOG1496 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00530000063410
HOGENOMiHOG000220953
HOVERGENiHBG006340
InParanoidiO88989
KOiK00025
OMAiDPMTAFK
OrthoDBiEOG091G0BLA
PhylomeDBiO88989
TreeFamiTF105826

Enzyme and pathway databases

ReactomeiR-RNO-70263 Gluconeogenesis

Miscellaneous databases

PROiPR:O88989

Gene expression databases

BgeeiENSRNOG00000008103 Expressed in 10 organ(s), highest expression level in heart
GenevisibleiO88989 RN

Family and domain databases

CDDicd01336 MDH_cytoplasmic_cytosolic, 1 hit
Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01517 Malate_dehydrog_2, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR011274 Malate_DH_NAD-dep_euk
IPR010945 Malate_DH_type2
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23382 PTHR23382, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01759 MalateDH-SF1, 1 hit
TIGR01758 MDH_euk_cyt, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMDHC_RAT
AccessioniPrimary (citable) accession number: O88989
Secondary accession number(s): O88585, Q6PCV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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