Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial

Gene

Gcat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate.

Cofactori

Pathwayi: L-threonine degradation via oxydo-reductase pathway

This protein is involved in step 2 of the subpathway that synthesizes glycine from L-threonine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-threonine 3-dehydrogenase, mitochondrial (Tdh)
  2. 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (Gcat)
This subpathway is part of the pathway L-threonine degradation via oxydo-reductase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-threonine, the pathway L-threonine degradation via oxydo-reductase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei156SubstrateBy similarity1
Binding sitei203Pyridoxal phosphateBy similarity1
Binding sitei386SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayi
UPA00046;UER00506

Names & Taxonomyi

Protein namesi
Recommended name:
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (EC:2.3.1.29)
Short name:
AKB ligase
Alternative name(s):
Aminoacetone synthase
Glycine acetyltransferase
Gene namesi
Name:Gcat
Synonyms:Kbl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1349389 Gcat

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 18MitochondrionSequence analysisAdd BLAST18
ChainiPRO_000000124719 – 4162-amino-3-ketobutyrate coenzyme A ligase, mitochondrialAdd BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-acetyllysine; alternateCombined sources1
Modified residuei42N6-succinyllysine; alternateCombined sources1
Modified residuei184N6-acetyllysine; alternateCombined sources1
Modified residuei184N6-succinyllysine; alternateCombined sources1
Modified residuei262N6-(pyridoxal phosphate)lysineCurated1
Modified residuei323N6-succinyllysineCombined sources1
Modified residuei365N6-succinyllysineCombined sources1
Modified residuei380N6-acetyllysine; alternateCombined sources1
Modified residuei380N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO88986
MaxQBiO88986
PaxDbiO88986
PeptideAtlasiO88986
PRIDEiO88986

2D gel databases

REPRODUCTION-2DPAGEiO88986
SWISS-2DPAGEiO88986

PTM databases

iPTMnetiO88986
PhosphoSitePlusiO88986
SwissPalmiO88986

Expressioni

Developmental stagei

Present in the placenta, brain and liver during embryonic development (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000006378 Expressed in 282 organ(s), highest expression level in pancreas
GenevisibleiO88986 MM

Interactioni

Protein-protein interaction databases

BioGridi205059, 1 interactor
STRINGi10090.ENSMUSP00000006544

Structurei

3D structure databases

ProteinModelPortaliO88986
SMRiO88986
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 132Pyridoxal phosphate bindingBy similarity2
Regioni259 – 262Pyridoxal phosphate bindingBy similarity4
Regioni292 – 293Pyridoxal phosphate binding; shared with dimeric partnerBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1359 Eukaryota
COG0156 LUCA
GeneTreeiENSGT00530000063111
HOGENOMiHOG000221022
HOVERGENiHBG105208
InParanoidiO88986
KOiK00639
OMAiMDTHGFG
OrthoDBiEOG091G041D
PhylomeDBiO88986
TreeFamiTF105923

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_00985 2am3keto_CoA_ligase, 1 hit
InterProiView protein in InterPro
IPR011282 2am3keto_CoA_ligase
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR13693:SF60 PTHR13693:SF60, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01822 2am3keto_CoA, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O88986-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWASFMWHGA LSPGRRAHSA LAQLRCILDS ELEGIRGAGT WKSERVITSR
60 70 80 90 100
QGPSIRVDGI SGGILNFCAN NYLGLSSHPA VIQAGLQTLE EFGAGLSSTR
110 120 130 140 150
FICGTQSIHK NLEAKIAHFH QREDAILYPS CFDANAGLFE ALLTPEDAVL
160 170 180 190 200
SDELNHASII DGIRLCKAHK YRYRHLDMAD LEAKLKEAQK HRLRLVATDG
210 220 230 240 250
AFSMDGDIAP LQDICRLAAQ YGALVFVDEC HATGFLGPTG RGTDELLGVM
260 270 280 290 300
DQVTIINSTL GKALGGASGG YTTGPEPLVS LLRQRSRPYL FSNSLPPAVV
310 320 330 340 350
GCASKALDLL MESNAIIQSM AAKTRRFRSK MEAAGFTVSG ADHPICPVML
360 370 380 390 400
GDARLSSQMA DDMLKKGIFV IGFSYPVVPK GKARIRVQIS AVHSEEDIDR
410
CVEAFVEVGR LHGALP
Length:416
Mass (Da):44,931
Last modified:January 1, 1999 - v2
Checksum:i01EA71B3846BEBBF
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PWY6E9PWY6_MOUSE
2-amino-3-ketobutyrate coenzyme A l...
Gcat
382Annotation score:
A0A2R8VHE4A0A2R8VHE4_MOUSE
2-amino-3-ketobutyrate coenzyme A l...
Gcat
227Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093403 mRNA Translation: AAC99774.1
AK087433 mRNA Translation: BAC39872.1
BC024107 mRNA Translation: AAH24107.1
CCDSiCCDS27630.1
RefSeqiNP_038875.3, NM_013847.4
UniGeneiMm.237085

Genome annotation databases

EnsembliENSMUST00000006544; ENSMUSP00000006544; ENSMUSG00000006378
GeneIDi26912
KEGGimmu:26912
UCSCiuc007wse.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093403 mRNA Translation: AAC99774.1
AK087433 mRNA Translation: BAC39872.1
BC024107 mRNA Translation: AAH24107.1
CCDSiCCDS27630.1
RefSeqiNP_038875.3, NM_013847.4
UniGeneiMm.237085

3D structure databases

ProteinModelPortaliO88986
SMRiO88986
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205059, 1 interactor
STRINGi10090.ENSMUSP00000006544

PTM databases

iPTMnetiO88986
PhosphoSitePlusiO88986
SwissPalmiO88986

2D gel databases

REPRODUCTION-2DPAGEiO88986
SWISS-2DPAGEiO88986

Proteomic databases

EPDiO88986
MaxQBiO88986
PaxDbiO88986
PeptideAtlasiO88986
PRIDEiO88986

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006544; ENSMUSP00000006544; ENSMUSG00000006378
GeneIDi26912
KEGGimmu:26912
UCSCiuc007wse.2 mouse

Organism-specific databases

CTDi23464
MGIiMGI:1349389 Gcat

Phylogenomic databases

eggNOGiKOG1359 Eukaryota
COG0156 LUCA
GeneTreeiENSGT00530000063111
HOGENOMiHOG000221022
HOVERGENiHBG105208
InParanoidiO88986
KOiK00639
OMAiMDTHGFG
OrthoDBiEOG091G041D
PhylomeDBiO88986
TreeFamiTF105923

Enzyme and pathway databases

UniPathwayi
UPA00046;UER00506

Miscellaneous databases

ChiTaRSiGcat mouse
PROiPR:O88986
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006378 Expressed in 282 organ(s), highest expression level in pancreas
GenevisibleiO88986 MM

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
HAMAPiMF_00985 2am3keto_CoA_ligase, 1 hit
InterProiView protein in InterPro
IPR011282 2am3keto_CoA_ligase
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR13693:SF60 PTHR13693:SF60, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01822 2am3keto_CoA, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKBL_MOUSE
AccessioniPrimary (citable) accession number: O88986
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1999
Last modified: November 7, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again