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Protein

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

Gene

Cyp8b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.By similarity

Catalytic activityi

7-alpha-hydroxycholest-4-en-3-one + 2 ferrocytochrome b5 + 2 H+ + O2 = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + 2 ferricytochrome b5 + + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi439Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (EC:1.14.18.8By similarity)
Alternative name(s):
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
CYPVIIIB1
Cytochrome P450 8B1
Sterol 12-alpha-hydroxylase
Gene namesi
Name:Cyp8b1
Synonyms:Cyp12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1338044 Cyp8b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519141 – 5007-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylaseAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei325PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO88962
PaxDbiO88962
PeptideAtlasiO88962
PRIDEiO88962

PTM databases

iPTMnetiO88962
PhosphoSitePlusiO88962
SwissPalmiO88962

Expressioni

Tissue specificityi

Expressed in liver.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052989

Structurei

3D structure databases

ProteinModelPortaliO88962
SMRiO88962
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
HOGENOMiHOG000231026
HOVERGENiHBG051100
InParanoidiO88962
KOiK07431
PhylomeDBiO88962
TreeFamiTF105090

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR024204 Cyt_P450_CYP7A1-type
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
IPR030686 Cytochrome_CYP8B1
PANTHERiPTHR24306:SF0 PTHR24306:SF0, 1 hit
PfamiView protein in Pfam
PF00067 p450, 1 hit
PIRSFiPIRSF500627 Cytochrome_CYP8B1, 1 hit
PIRSF000047 Cytochrome_CYPVIIA1, 1 hit
PRINTSiPR00465 EP450IV
SUPFAMiSSF48264 SSF48264, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O88962-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLWCTVLGA LLTVVGCLCL SLLLRHRRPW EPPLDKGFVP WLGHSMAFRK
60 70 80 90 100
NMFEFLKGMR AKHGDVFTVQ LGGQYFTFVM DPLSFGPIIK NTEKALDFQS
110 120 130 140 150
YAKELVLKVF GYQSVDGDHR MIHLASTKHL MGQGLEELNQ AMLDSLSLVM
160 170 180 190 200
LGPKGSSLGA SSWCEDGLFH FCYRILFKAG FLSLFGYTKD KQQDLDEADE
210 220 230 240 250
LFRKFRRFDF LFPRFVYSLL GPREWVEVSQ LQRLFHQRLS VEQNLEKDGI
260 270 280 290 300
SCWLGYMLQF LREQGIASSM QDKFNFMMLW ASQGNTGPTC FWVLLFLLKH
310 320 330 340 350
QDAMKAVREE ATRVMGKARL EAKKSFTFTP SALKHTPVLD SVMEESLRLC
360 370 380 390 400
ATPTLLRVVQ EDYVLKMASG QEYQIRRGDK VALFPYLSVH MDPDIHPEPT
410 420 430 440 450
AFKYDRFLNP DGTRKVDFYK SGKKIHHYSM PWGSGVSKCP GRFFALSEMK
460 470 480 490 500
TFVLLMIMYF DFKLVDPDIP VPPIDPRRWG FGTSQPSHEV RFLYRLKPVQ
Length:500
Mass (Da):57,706
Last modified:November 1, 1998 - v1
Checksum:iD6902755B4AD141A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J0N7A0A0R4J0N7_MOUSE
7-alpha-hydroxycholest-4-en-3-one 1...
Cyp8b1
500Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti242E → K in strain: 129/SvJ. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090317 mRNA Translation: AAC63036.1
AF090319 Genomic DNA Translation: AAD19876.1
BC010973 mRNA Translation: AAH10973.1
BC010974 mRNA Translation: AAH10974.1
BC049969 mRNA Translation: AAH49969.1
CCDSiCCDS23641.1
RefSeqiNP_034142.3, NM_010012.3
UniGeneiMm.20889

Genome annotation databases

GeneIDi13124
KEGGimmu:13124
UCSCiuc009sef.2 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090317 mRNA Translation: AAC63036.1
AF090319 Genomic DNA Translation: AAD19876.1
BC010973 mRNA Translation: AAH10973.1
BC010974 mRNA Translation: AAH10974.1
BC049969 mRNA Translation: AAH49969.1
CCDSiCCDS23641.1
RefSeqiNP_034142.3, NM_010012.3
UniGeneiMm.20889

3D structure databases

ProteinModelPortaliO88962
SMRiO88962
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052989

PTM databases

iPTMnetiO88962
PhosphoSitePlusiO88962
SwissPalmiO88962

Proteomic databases

MaxQBiO88962
PaxDbiO88962
PeptideAtlasiO88962
PRIDEiO88962

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13124
KEGGimmu:13124
UCSCiuc009sef.2 mouse

Organism-specific databases

CTDi1582
MGIiMGI:1338044 Cyp8b1

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
HOGENOMiHOG000231026
HOVERGENiHBG051100
InParanoidiO88962
KOiK07431
PhylomeDBiO88962
TreeFamiTF105090

Miscellaneous databases

PROiPR:O88962
SOURCEiSearch...

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR024204 Cyt_P450_CYP7A1-type
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
IPR030686 Cytochrome_CYP8B1
PANTHERiPTHR24306:SF0 PTHR24306:SF0, 1 hit
PfamiView protein in Pfam
PF00067 p450, 1 hit
PIRSFiPIRSF500627 Cytochrome_CYP8B1, 1 hit
PIRSF000047 Cytochrome_CYPVIIA1, 1 hit
PRINTSiPR00465 EP450IV
SUPFAMiSSF48264 SSF48264, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCP8B1_MOUSE
AccessioniPrimary (citable) accession number: O88962
Secondary accession number(s): Q9R217
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: June 20, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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