UniProtKB - O88947 (FA10_MOUSE)
Protein
Coagulation factor X
Gene
F10
Organism
Mus musculus (Mouse)
Status
Functioni
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Catalytic activityi
- Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin. EC:3.4.21.6
Activity regulationi
Inhibited by SERPINA5.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 273 | Charge relay systemBy similarity | 1 | |
Active sitei | 319 | Charge relay systemBy similarity | 1 | |
Active sitei | 416 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipid binding Source: MGI
- serine-type endopeptidase activity Source: MGI
GO - Biological processi
- blood coagulation Source: MGI
- positive regulation of protein kinase B signaling Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Blood coagulation, Hemostasis |
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-MMU-140834, Extrinsic Pathway of Fibrin Clot Formation R-MMU-140837, Intrinsic Pathway of Fibrin Clot Formation R-MMU-140875, Common Pathway of Fibrin Clot Formation R-MMU-159740, Gamma-carboxylation of protein precursors R-MMU-159763, Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus R-MMU-159782, Removal of aminoterminal propeptides from gamma-carboxylated proteins |
Protein family/group databases
MEROPSi | S01.216 |
Names & Taxonomyi
Protein namesi | Recommended name: Coagulation factor X (EC:3.4.21.6)Alternative name(s): Stuart factor Cleaved into the following 3 chains: |
Gene namesi | Name:F10 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103107, F10 |
Subcellular locationi
Extracellular region or secreted
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
PropeptideiPRO_0000027792 | 21 – 40 | By similarityAdd BLAST | 20 | |
ChainiPRO_0000027793 | 41 – 481 | Coagulation factor XAdd BLAST | 441 | |
ChainiPRO_0000027794 | 41 – 180 | Factor X light chainBy similarityAdd BLAST | 140 | |
ChainiPRO_0000027795 | 184 – 481 | Factor X heavy chainBy similarityAdd BLAST | 298 | |
PropeptideiPRO_0000027796 | 184 – 231 | Activation peptideBy similarityAdd BLAST | 48 | |
ChainiPRO_0000027797 | 232 – 481 | Activated factor Xa heavy chainBy similarityAdd BLAST | 250 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 46 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 47 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 54 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 56 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Disulfide bondi | 57 ↔ 62 | By similarity | ||
Modified residuei | 59 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 60 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 65 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 66 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 69 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 72 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 75 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 79 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Disulfide bondi | 90 ↔ 101 | By similarity | ||
Disulfide bondi | 95 ↔ 110 | By similarity | ||
Modified residuei | 103 | (3R)-3-hydroxyaspartateBy similarity | 1 | |
Disulfide bondi | 112 ↔ 121 | By similarity | ||
Disulfide bondi | 129 ↔ 140 | By similarity | ||
Disulfide bondi | 136 ↔ 149 | By similarity | ||
Disulfide bondi | 151 ↔ 164 | By similarity | ||
Disulfide bondi | 172 ↔ 339 | Interchain (between light and heavy chains)PROSITE-ProRule annotation | ||
Glycosylationi | 187 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 218 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 238 ↔ 243 | By similarity | ||
Disulfide bondi | 258 ↔ 274 | By similarity | ||
Disulfide bondi | 387 ↔ 401 | By similarity | ||
Disulfide bondi | 412 ↔ 440 | By similarity |
Post-translational modificationi
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.By similarity
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).By similarity
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, Glycoprotein, Hydroxylation, ZymogenProteomic databases
CPTACi | non-CPTAC-3413 |
jPOSTi | O88947 |
PaxDbi | O88947 |
PeptideAtlasi | O88947 |
PRIDEi | O88947 |
ProteomicsDBi | 275832 |
PTM databases
GlyGeni | O88947, 2 sites |
iPTMneti | O88947 |
PhosphoSitePlusi | O88947 |
Expressioni
Tissue specificityi
Plasma; synthesized in the liver.
Gene expression databases
Bgeei | ENSMUSG00000031444, Expressed in liver and 115 other tissues |
ExpressionAtlasi | O88947, baseline and differential |
Genevisiblei | O88947, MM |
Interactioni
Subunit structurei
The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds.
Forms a heterodimer with SERPINA5 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 199566, 10 interactors |
IntActi | O88947, 2 interactors |
STRINGi | 10090.ENSMUSP00000068389 |
Miscellaneous databases
RNActi | O88947, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 85 | GlaPROSITE-ProRule annotationAdd BLAST | 45 | |
Domaini | 86 – 122 | EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 125 – 165 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 41 | |
Domaini | 232 – 464 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 233 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QS4N, Eukaryota |
GeneTreei | ENSGT00940000157694 |
HOGENOMi | CLU_006842_19_5_1 |
InParanoidi | O88947 |
TreeFami | TF327329 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 4.10.740.10, 1 hit |
InterProi | View protein in InterPro IPR017857, Coagulation_fac-like_Gla_dom IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR035972, GLA-like_dom_SF IPR000294, GLA_domain IPR012224, Pept_S1A_FX IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00594, Gla, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001143, Factor_X, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN PR00001, GLABLOOD |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 1 hit SM00069, GLA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57630, SSF57630, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 2 hits PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00011, GLA_1, 1 hit PS50998, GLA_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
O88947-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSPVQLSLL CVVLASLLLP GKGVFINRER ANNVLARTRR ANSFFEEFKK
60 70 80 90 100
GNLERECMEE ICSYEEVREI FEDDEKTKEY WTKYKDGDQC ESSPCQNQGA
110 120 130 140 150
CRDGIGGYTC TCSEGFEGKN CELFVRKLCR LDNGDCDQFC REEQNSVVCS
160 170 180 190 200
CASGYFLGND GKSCISTAPF PCGKITTGRR KRSVALNTSD SELDLEDALL
210 220 230 240 250
DEDFLSPTEN PIELLNLNET QPERSSDDLV RIVGGRECKD GECPWQALLI
260 270 280 290 300
NEDNEGFCGG TILNEFYILT AAHCLHQARR FKVRVGDRNT EKEEGNEMVH
310 320 330 340 350
EVDVVIKHNK FQRDTYDYDI AVLRLKTPIT FRMNVAPACL PQKDWAESTL
360 370 380 390 400
MTQKTGIVSG FGRTHEKGRQ SNILKMLEVP YVDRNTCKLS TSFSITQNMF
410 420 430 440 450
CAGYEAKLED ACQGDSGGPH VTRFKNTYYV TGIVSWGEGC ARKGKYGIYT
460 470 480
KVTTFLKWID RSMKARVGPT AETPRTAGPP N
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3U3V1 | Q3U3V1_MOUSE | Coagulation factor X | F10 | 493 | Annotation score: | ||
D3Z215 | D3Z215_MOUSE | Coagulation factor X | F10 | 321 | Annotation score: | ||
D3Z521 | D3Z521_MOUSE | Coagulation factor X | F10 | 319 | Annotation score: | ||
D3Z7R3 | D3Z7R3_MOUSE | Coagulation factor X | F10 | 119 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 250 | I → V in AAH03877 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 294 | E → D in CAA10933 (PubMed:9783672).Curated | 1 | |
Sequence conflicti | 298 | M → L in CAA10933 (PubMed:9783672).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF087644 mRNA Translation: AAC36345.1 AJ222677 mRNA Translation: CAA10933.1 AF211347 Genomic DNA Translation: AAF22980.1 BC003877 mRNA Translation: AAH03877.1 |
CCDSi | CCDS40226.1 |
RefSeqi | NP_031998.3, NM_007972.4 |
Genome annotation databases
Ensembli | ENSMUST00000063820; ENSMUSP00000068389; ENSMUSG00000031444 |
GeneIDi | 14058 |
KEGGi | mmu:14058 |
UCSCi | uc009kws.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF087644 mRNA Translation: AAC36345.1 AJ222677 mRNA Translation: CAA10933.1 AF211347 Genomic DNA Translation: AAF22980.1 BC003877 mRNA Translation: AAH03877.1 |
CCDSi | CCDS40226.1 |
RefSeqi | NP_031998.3, NM_007972.4 |
3D structure databases
SMRi | O88947 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199566, 10 interactors |
IntActi | O88947, 2 interactors |
STRINGi | 10090.ENSMUSP00000068389 |
Protein family/group databases
MEROPSi | S01.216 |
PTM databases
GlyGeni | O88947, 2 sites |
iPTMneti | O88947 |
PhosphoSitePlusi | O88947 |
Proteomic databases
CPTACi | non-CPTAC-3413 |
jPOSTi | O88947 |
PaxDbi | O88947 |
PeptideAtlasi | O88947 |
PRIDEi | O88947 |
ProteomicsDBi | 275832 |
Protocols and materials databases
Antibodypediai | 11687, 677 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000063820; ENSMUSP00000068389; ENSMUSG00000031444 |
GeneIDi | 14058 |
KEGGi | mmu:14058 |
UCSCi | uc009kws.2, mouse |
Organism-specific databases
CTDi | 2159 |
MGIi | MGI:103107, F10 |
Phylogenomic databases
eggNOGi | ENOG502QS4N, Eukaryota |
GeneTreei | ENSGT00940000157694 |
HOGENOMi | CLU_006842_19_5_1 |
InParanoidi | O88947 |
TreeFami | TF327329 |
Enzyme and pathway databases
Reactomei | R-MMU-140834, Extrinsic Pathway of Fibrin Clot Formation R-MMU-140837, Intrinsic Pathway of Fibrin Clot Formation R-MMU-140875, Common Pathway of Fibrin Clot Formation R-MMU-159740, Gamma-carboxylation of protein precursors R-MMU-159763, Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus R-MMU-159782, Removal of aminoterminal propeptides from gamma-carboxylated proteins |
Miscellaneous databases
BioGRID-ORCSi | 14058, 2 hits in 52 CRISPR screens |
ChiTaRSi | F10, mouse |
PROi | PR:O88947 |
RNActi | O88947, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031444, Expressed in liver and 115 other tissues |
ExpressionAtlasi | O88947, baseline and differential |
Genevisiblei | O88947, MM |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 4.10.740.10, 1 hit |
InterProi | View protein in InterPro IPR017857, Coagulation_fac-like_Gla_dom IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR035972, GLA-like_dom_SF IPR000294, GLA_domain IPR012224, Pept_S1A_FX IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00594, Gla, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001143, Factor_X, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN PR00001, GLABLOOD |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 1 hit SM00069, GLA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57630, SSF57630, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 2 hits PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00011, GLA_1, 1 hit PS50998, GLA_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FA10_MOUSE | |
Accessioni | O88947Primary (citable) accession number: O88947 Secondary accession number(s): O54740, Q99L32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | November 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families