UniProtKB - O88939 (ZBT7A_MOUSE)
Zinc finger and BTB domain-containing protein 7A
Zbtb7a
Functioni
Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation (PubMed:15337766, PubMed:15662416, PubMed:17495164, PubMed:26816381, PubMed:29813070).
Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions (PubMed:15337766, PubMed:15662416, PubMed:26816381, PubMed:29813070).
Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms (By similarity).
That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300 (By similarity).
Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors (PubMed:29813070).
Also directly interacts with transcription factors like SP1 to prevent their binding to DNA (By similarity).
Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes (By similarity).
Thereby, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (By similarity).
Involved in the switch between fetal and adult globin expression during erythroid cells maturation (PubMed:26816381).
Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription (PubMed:26816381).
Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene (PubMed:15662416).
Efficiently abrogates E2F1-dependent CDKN2A transactivation (PubMed:15662416).
Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation (PubMed:15337766).
Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis (By similarity).
Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation (By similarity).
Plays a key role in the differentiation of lymphoid progenitors into B and T lineages (PubMed:17495164).
Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes (PubMed:17495164).
Also regulates osteoclast differentiation (By similarity).
May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ (PubMed:26446488).
Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair (PubMed:26446488).
May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1 (By similarity).
By similarity6 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 376 – 398 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 404 – 426 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 432 – 454 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 460 – 484 | C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription factor binding Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- histone acetyltransferase binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
- SMAD binding Source: UniProtKB
- transcription corepressor binding Source: UniProtKB
GO - Biological processi
- B cell differentiation Source: UniProtKB
- cartilage development Source: UniProtKB
- chromatin organization Source: UniProtKB
- chromatin remodeling Source: UniProtKB
- double-strand break repair via classical nonhomologous end joining Source: UniProtKB
- erythrocyte maturation Source: UniProtKB
- fat cell differentiation Source: UniProtKB
- negative regulation of androgen receptor signaling pathway Source: UniProtKB
- negative regulation of Notch signaling pathway Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- negative regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- protein localization to nucleus Source: MGI
- regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
- regulation of apoptotic process Source: UniProtKB
- regulation of DNA-binding transcription factor activity Source: UniProtKB
- regulation of glycolytic process Source: UniProtKB
- regulation of osteoclast differentiation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription regulatory region DNA binding Source: UniProtKB
- transcription, DNA-templated Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, DNA damage, DNA repair, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger and BTB domain-containing protein 7ACuratedAlternative name(s): Leukemia/lymphoma-related factor1 Publication POZ and Krueppel erythroid myeloid ontogenic factor1 Publication Short name: POK erythroid myeloid ontogenic factor1 Publication Short name: Pokemon1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1335091, Zbtb7a |
VEuPathDBi | HostDB:ENSMUSG00000035011 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Note: Recruited to double-strand break sites of damaged DNA.1 Publication
Nucleus
- DNA-dependent protein kinase complex Source: Ensembl
- nucleus Source: UniProtKB
- NuRD complex Source: Ensembl
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047716 | 1 – 569 | Zinc finger and BTB domain-containing protein 7AAdd BLAST | 569 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 331 | PhosphoserineCombined sources | 1 | |
Modified residuei | 335 | PhosphoserineBy similarity | 1 | |
Cross-linki | 430 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 527 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 537 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O88939 |
jPOSTi | O88939 |
MaxQBi | O88939 |
PaxDbi | O88939 |
PeptideAtlasi | O88939 |
PRIDEi | O88939 |
ProteomicsDBi | 302106 [O88939-1] 302107 [O88939-2] |
PTM databases
iPTMneti | O88939 |
PhosphoSitePlusi | O88939 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000035011, Expressed in adult mammalian kidney and 300 other tissues |
Genevisiblei | O88939, MM |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with BCL6 (PubMed:9927193).
Interacts with RELA; involved in the control by RELA of the accessibility of target gene promoters (PubMed:29813070).
Interacts with AR (via NR LBD domain); the interaction is direct and androgen-dependent (By similarity).
Interacts with NCOR1 (By similarity).
Interacts with NCOR2 (By similarity).
Interacts with SMAD4; the interaction is direct and stimulated by TGFB1 (By similarity).
Interacts with HDAC1 (By similarity).
Interacts with SP1; ZBTB7A prevents the binding to GC-rich motifs in promoters and represses the transcriptional activity of SP1 (By similarity).
Interacts with the DNA-dependent protein kinase complex/DNA-PKc (PubMed:26446488).
Interacts with KHDRBS1; negatively regulates KHDRBS1 splicing activity (By similarity).
By similarity3 PublicationsBinary interactionsi
O88939
With | #Exp. | IntAct |
---|---|---|
Hdac1 [O09106] | 3 | EBI-595063,EBI-301912 |
GO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- DNA-binding transcription factor binding Source: MGI
- histone acetyltransferase binding Source: UniProtKB
- SMAD binding Source: UniProtKB
- transcription corepressor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 201199, 10 interactors |
DIPi | DIP-33318N |
IntActi | O88939, 9 interactors |
STRINGi | 10090.ENSMUSP00000047333 |
Miscellaneous databases
RNActi | O88939, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 101 | BTBPROSITE-ProRule annotationAdd BLAST | 68 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 214 – 304 | DisorderedSequence analysisAdd BLAST | 91 | |
Regioni | 275 – 569 | Mediates interaction with KHDRBS1By similarityAdd BLAST | 295 | |
Regioni | 343 – 569 | Mediates interaction with RELA1 PublicationAdd BLAST | 227 | |
Regioni | 371 – 569 | Mediates interaction with SMAD4By similarityAdd BLAST | 199 | |
Regioni | 480 – 569 | DisorderedSequence analysisAdd BLAST | 90 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 376 – 398 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 404 – 426 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 432 – 454 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 460 – 484 | C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162053 |
HOGENOMi | CLU_025627_1_0_1 |
InParanoidi | O88939 |
OMAi | CKVRFTQ |
OrthoDBi | 1318335at2759 |
PhylomeDBi | O88939 |
TreeFami | TF331824 |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGGVDGPIG IPFPDHSSDI LSGLNEQRTQ GLLCDVVILV EGREFPTHRS
60 70 80 90 100
VLAACSQYFK KLFTSGAVVD QQNVYEIDFV SAEALTALMD FAYTATLTVS
110 120 130 140 150
TANVGDILSA ARLLEIPAVS HVCADLLERQ ILAADDVGDA SQPDGAGPTD
160 170 180 190 200
QRNLLRAKEY LEFFRSNPMN SLPPTAFPWS GFGAPDDDLD ATKEAVAAAV
210 220 230 240 250
AAVAAGDCNG LDFYGPGPPA DRPPAGDGDE GDSTPGLWPE RDEDAPPGGL
260 270 280 290 300
FPPPTAPPAT TQNGHYGRAG AGTGEEEAAA LSEAAPEPGD SPGFLSGAAE
310 320 330 340 350
GEDGDAADVD GLAASTLLQQ MMSSVGRAGD SDEESRTDDK GVMDYYLKYF
360 370 380 390 400
SGAHEGDVYP AWSQKGEKKI RAKAFQKCPI CEKVIQGAGK LPRHIRTHTG
410 420 430 440 450
EKPYECNICK VRFTRQDKLK VHMRKHTGEK PYLCQQCGAA FAHNYDLKNH
460 470 480 490 500
MRVHTGLRPY QCDSCCKTFV RSDHLHRHLK KDGCNGVPSR RGRKPRVRGV
510 520 530 540 550
PPDVPAGAGA PPGLPDAPRN GQEKHFKDEE EDEEEASPDG SGRLNVAGSG
560
GDDGAGGPAV ATAEGNFAT
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z4C3 | D3Z4C3_MOUSE | Zinc finger and BTB domain-containi... | Zbtb7a | 415 | Annotation score: | ||
D3YVF8 | D3YVF8_MOUSE | Zinc finger and BTB domain-containi... | Zbtb7a | 174 | Annotation score: | ||
D3YZI8 | D3YZI8_MOUSE | Zinc finger and BTB domain-containi... | Zbtb7a | 44 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 433 | L → M in BAB26897 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 548 | G → D in BAE32917 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_035026 | 532 – 535 | Missing in isoform 2. 1 Publication | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF086830 mRNA Translation: AAC35367.1 AK010379 mRNA Translation: BAB26897.1 AK154907 mRNA Translation: BAE32917.1 CH466553 Genomic DNA Translation: EDL31455.1 BC057204 mRNA Translation: AAH57204.1 BC138524 mRNA Translation: AAI38525.1 BC145311 mRNA Translation: AAI45312.1 |
CCDSi | CCDS35991.1 [O88939-1] |
RefSeqi | NP_034861.3, NM_010731.3 [O88939-1] XP_006513342.2, XM_006513279.2 [O88939-1] XP_006513343.2, XM_006513280.3 [O88939-1] XP_006513345.1, XM_006513282.2 [O88939-1] |
Genome annotation databases
Ensembli | ENSMUST00000048128; ENSMUSP00000047333; ENSMUSG00000035011 [O88939-1] ENSMUST00000117956; ENSMUSP00000113428; ENSMUSG00000035011 [O88939-1] ENSMUST00000119606; ENSMUSP00000113612; ENSMUSG00000035011 [O88939-1] |
GeneIDi | 16969 |
KEGGi | mmu:16969 |
UCSCi | uc007gfz.1, mouse [O88939-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF086830 mRNA Translation: AAC35367.1 AK010379 mRNA Translation: BAB26897.1 AK154907 mRNA Translation: BAE32917.1 CH466553 Genomic DNA Translation: EDL31455.1 BC057204 mRNA Translation: AAH57204.1 BC138524 mRNA Translation: AAI38525.1 BC145311 mRNA Translation: AAI45312.1 |
CCDSi | CCDS35991.1 [O88939-1] |
RefSeqi | NP_034861.3, NM_010731.3 [O88939-1] XP_006513342.2, XM_006513279.2 [O88939-1] XP_006513343.2, XM_006513280.3 [O88939-1] XP_006513345.1, XM_006513282.2 [O88939-1] |
3D structure databases
AlphaFoldDBi | O88939 |
SMRi | O88939 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201199, 10 interactors |
DIPi | DIP-33318N |
IntActi | O88939, 9 interactors |
STRINGi | 10090.ENSMUSP00000047333 |
PTM databases
iPTMneti | O88939 |
PhosphoSitePlusi | O88939 |
Proteomic databases
EPDi | O88939 |
jPOSTi | O88939 |
MaxQBi | O88939 |
PaxDbi | O88939 |
PeptideAtlasi | O88939 |
PRIDEi | O88939 |
ProteomicsDBi | 302106 [O88939-1] 302107 [O88939-2] |
Protocols and materials databases
Antibodypediai | 11338, 348 antibodies from 38 providers |
DNASUi | 16969 |
Genome annotation databases
Ensembli | ENSMUST00000048128; ENSMUSP00000047333; ENSMUSG00000035011 [O88939-1] ENSMUST00000117956; ENSMUSP00000113428; ENSMUSG00000035011 [O88939-1] ENSMUST00000119606; ENSMUSP00000113612; ENSMUSG00000035011 [O88939-1] |
GeneIDi | 16969 |
KEGGi | mmu:16969 |
UCSCi | uc007gfz.1, mouse [O88939-1] |
Organism-specific databases
CTDi | 51341 |
MGIi | MGI:1335091, Zbtb7a |
VEuPathDBi | HostDB:ENSMUSG00000035011 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000162053 |
HOGENOMi | CLU_025627_1_0_1 |
InParanoidi | O88939 |
OMAi | CKVRFTQ |
OrthoDBi | 1318335at2759 |
PhylomeDBi | O88939 |
TreeFami | TF331824 |
Miscellaneous databases
BioGRID-ORCSi | 16969, 18 hits in 74 CRISPR screens |
ChiTaRSi | Zbtb7a, mouse |
PROi | PR:O88939 |
RNActi | O88939, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000035011, Expressed in adult mammalian kidney and 300 other tissues |
Genevisiblei | O88939, MM |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZBT7A_MOUSE | |
Accessioni | O88939Primary (citable) accession number: O88939 Secondary accession number(s): B2RRP7 Q9CRJ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | September 2, 2008 | |
Last modified: | May 25, 2022 | |
This is version 188 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot