UniProtKB - O88935 (SYN1_MOUSE)
Protein
Synapsin-1
Gene
Syn1
Organism
Mus musculus (Mouse)
Status
Functioni
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- ATP binding Source: InterPro
- calcium-dependent protein binding Source: MGI
- identical protein binding Source: MGI
- protein kinase binding Source: ParkinsonsUK-UCL
GO - Biological processi
- neuron development Source: Ensembl
- neurotransmitter secretion Source: MGI
- regulation of short-term neuronal synaptic plasticity Source: ParkinsonsUK-UCL
- regulation of synaptic vesicle cycle Source: SynGO
- synapse organization Source: MGI
- synaptic vesicle clustering Source: SynGO
- synaptic vesicle cycle Source: SynGO
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
Reactomei | R-MMU-181429, Serotonin Neurotransmitter Release Cycle R-MMU-212676, Dopamine Neurotransmitter Release Cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Synapsin-1Alternative name(s): Synapsin I |
Gene namesi | Name:Syn1 Synonyms:Syn-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98460, Syn1 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 1 Publication
Other locations
- synapse 1 Publication
Cytoskeleton
- cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Nucleus
- synaptonemal complex Source: MGI
Other locations
- anchored component of synaptic vesicle membrane Source: MGI
- axon Source: MGI
- dendrite Source: MGI
- extrinsic component of synaptic vesicle membrane Source: MGI
- intracellular organelle Source: MGI
- myelin sheath Source: UniProtKB
- postsynaptic density Source: MGI
- presynapse Source: MGI
- presynaptic active zone Source: MGI
- Schaffer collateral - CA1 synapse Source: SynGO
- synapse Source: MGI
- synaptic vesicle Source: MGI
- synaptic vesicle membrane Source: MGI
Keywords - Cellular componenti
Cell junction, Golgi apparatus, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000183019 | 1 – 706 | Synapsin-1Add BLAST | 706 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphoserineCombined sources | 1 | |
Modified residuei | 39 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 55 | O-linked (GlcNAc) serineBy similarity | 1 | |
Glycosylationi | 56 | O-linked (GlcNAc) threonineBy similarity | 1 | |
Modified residuei | 62 | PhosphoserineBy similarity | 1 | |
Modified residuei | 67 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 87 | O-linked (GlcNAc) threonine1 Publication | 1 | |
Glycosylationi | 96 | O-linked (GlcNAc) serineBy similarity | 1 | |
Glycosylationi | 103 | O-linked (GlcNAc) serineBy similarity | 1 | |
Glycosylationi | 261 | O-linked (GlcNAc) serineBy similarity | 1 | |
Modified residuei | 312 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 427 | PhosphoserineCombined sources | 1 | |
Modified residuei | 430 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 432 | Phosphoserine; alternateCombined sources | 1 | |
Glycosylationi | 432 | O-linked (GlcNAc) serine; alternateBy similarity | 1 | |
Modified residuei | 434 | PhosphoserineCombined sources | 1 | |
Modified residuei | 437 | PhosphoserineCombined sources | 1 | |
Modified residuei | 438 | PhosphoserineCombined sources | 1 | |
Modified residuei | 476 | Omega-N-methylarginineCombined sources | 1 | |
Glycosylationi | 518 | O-linked (GlcNAc) serineBy similarity | 1 | |
Glycosylationi | 526 | O-linked (GlcNAc) threonine1 Publication | 1 | |
Modified residuei | 534 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 547 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 551 | Phosphoserine; by PDPK1By similarity | 1 | |
Modified residuei | 553 | PhosphoserineBy similarity | 1 | |
Modified residuei | 556 | Omega-N-methylarginineCombined sources | 1 | |
Glycosylationi | 564 | O-linked (GlcNAc) threonineBy similarity | 1 | |
Modified residuei | 568 | Phosphoserine; by CaMK2By similarity | 1 | |
Glycosylationi | 578 | O-linked (GlcNAc) serineBy similarity | 1 | |
Modified residuei | 605 | Phosphoserine; by CaMK2By similarity | 1 | |
Modified residuei | 622 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 664 | PhosphoserineCombined sources | 1 | |
Modified residuei | 666 | PhosphoserineCombined sources | 1 | |
Modified residuei | 680 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 684 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal (By similarity).By similarity
Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.By similarity
Keywords - PTMi
Glycoprotein, Methylation, PhosphoproteinProteomic databases
PaxDbi | O88935 |
PeptideAtlasi | O88935 |
PRIDEi | O88935 |
PTM databases
GlyGeni | O88935, 11 sites |
iPTMneti | O88935 |
PhosphoSitePlusi | O88935 |
SwissPalmi | O88935 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000037217, Expressed in hypothalamus and 147 other tissues |
Genevisiblei | O88935, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts with CAPON.
Forms a ternary complex with NOS1 (By similarity). Isoform Ib interacts with PRNP.
By similarity1 PublicationGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- calcium-dependent protein binding Source: MGI
- identical protein binding Source: MGI
- protein kinase binding Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 203600, 33 interactors |
CORUMi | O88935 |
IntActi | O88935, 15 interactors |
MINTi | O88935 |
STRINGi | 10090.ENSMUSP00000111002 |
Miscellaneous databases
RNActi | O88935, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 28 | AAdd BLAST | 28 | |
Regioni | 29 – 112 | B; linkerAdd BLAST | 84 | |
Regioni | 113 – 420 | C; actin-binding and synaptic-vesicle bindingAdd BLAST | 308 | |
Regioni | 421 – 657 | D; Pro-rich linkerAdd BLAST | 237 | |
Regioni | 658 – 706 | EAdd BLAST | 49 |
Domaini
The A region binds phospholipids with a preference for negatively charged species.By similarity
Sequence similaritiesi
Belongs to the synapsin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3895, Eukaryota |
GeneTreei | ENSGT00940000161978 |
HOGENOMi | CLU_010582_3_0_1 |
InParanoidi | O88935 |
OMAi | APMTQGQ |
OrthoDBi | 799740at2759 |
PhylomeDBi | O88935 |
TreeFami | TF319919 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR028713, SYN1 IPR001359, Synapsin IPR020898, Synapsin_ATP-bd_dom IPR019735, Synapsin_CS IPR019736, Synapsin_P_site IPR020897, Synapsin_pre-ATP-grasp_dom |
PANTHERi | PTHR10841:SF24, PTHR10841:SF24, 1 hit |
Pfami | View protein in Pfam PF02078, Synapsin, 1 hit PF02750, Synapsin_C, 1 hit PF10581, Synapsin_N, 1 hit |
PRINTSi | PR01368, SYNAPSIN |
SUPFAMi | SSF52440, SSF52440, 1 hit |
PROSITEi | View protein in PROSITE PS00415, SYNAPSIN_1, 1 hit PS00416, SYNAPSIN_2, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform Ia (identifier: O88935-2) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPSAASP GATPGSATAS
60 70 80 90 100
AERASTAAPV ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG
110 120 130 140 150
GGSGGAGRGG AAARVLLVID EPHTDWAKYF KGKKIHGEID IKVEQAEFSD
160 170 180 190 200
LNLVAHANGG FSVDMEVLRN GVKVVRSLKP DFVLIRQHAF SMARNGDYRS
210 220 230 240 250
LVIGLQYAGI PSVNSLHSVY NFCDKPWVFA QMVRLHKKLG TEEFPLIDQT
260 270 280 290 300
FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT
310 320 330 340 350
YATAEPFIDA KYDVRVQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS
360 370 380 390 400
DRYKLWVDTC SEIFGGLDIC AVEALHGKDG RDHIIEVVGS SMPLIGDHQD
410 420 430 440 450
EDKQLIVELV VNKMTQALPR QPQRDASPGR GSHSQSSSPG ALTLGRQTSQ
460 470 480 490 500
QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP LQQRPPPQGQ QHLSGLGPPA
510 520 530 540 550
GSPLPQRLPS PTAAPQQSAS QATPVTQGQG RQSRPVAGGP GAPPAARPPA
560 570 580 590 600
SPSPQRQAGA PQATRQASIS GPAPTKASGA PPGGQQRQGP PQKPPGPAGP
610 620 630 640 650
TRQASQAGPG PRTGPPTTQQ PRPSGPGPAG RPAKPQLAQK PSQDVPPPIT
660 670 680 690 700
AAAGGPPHPQ LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF
ASLFSD
Isoform Ib (identifier: O88935-1) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
662-670: NKSQSLTNA → KASPSQAQP
671-706: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 44 | P → L in AAA79963 (PubMed:8034599).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015205 | 573 – 600 | Missing in isoform 3. 1 PublicationAdd BLAST | 28 | |
Alternative sequenceiVSP_015206 | 662 – 670 | NKSQSLTNA → KASPSQAQP in isoform Ib. 1 Publication | 9 | |
Alternative sequenceiVSP_015207 | 671 – 706 | Missing in isoform Ib. 1 PublicationAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF085809 mRNA Translation: AAD09833.1 BC022954 mRNA Translation: AAH22954.1 L32025 Genomic DNA Translation: AAA79963.1 |
CCDSi | CCDS53017.1 [O88935-2] CCDS53018.1 [O88935-1] |
PIRi | A53692 |
RefSeqi | NP_001104250.1, NM_001110780.1 [O88935-1] NP_038708.3, NM_013680.4 [O88935-2] |
Genome annotation databases
Ensembli | ENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217 [O88935-2] ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217 [O88935-1] |
GeneIDi | 20964 |
KEGGi | mmu:20964 |
UCSCi | uc009stw.2, mouse [O88935-2] uc009stx.2, mouse [O88935-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF085809 mRNA Translation: AAD09833.1 BC022954 mRNA Translation: AAH22954.1 L32025 Genomic DNA Translation: AAA79963.1 |
CCDSi | CCDS53017.1 [O88935-2] CCDS53018.1 [O88935-1] |
PIRi | A53692 |
RefSeqi | NP_001104250.1, NM_001110780.1 [O88935-1] NP_038708.3, NM_013680.4 [O88935-2] |
3D structure databases
SMRi | O88935 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203600, 33 interactors |
CORUMi | O88935 |
IntActi | O88935, 15 interactors |
MINTi | O88935 |
STRINGi | 10090.ENSMUSP00000111002 |
PTM databases
GlyGeni | O88935, 11 sites |
iPTMneti | O88935 |
PhosphoSitePlusi | O88935 |
SwissPalmi | O88935 |
Proteomic databases
PaxDbi | O88935 |
PeptideAtlasi | O88935 |
PRIDEi | O88935 |
Protocols and materials databases
ABCDi | O88935, 1 sequenced antibody |
Antibodypediai | 403, 1044 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000081893; ENSMUSP00000080568; ENSMUSG00000037217 [O88935-2] ENSMUST00000115345; ENSMUSP00000111002; ENSMUSG00000037217 [O88935-1] |
GeneIDi | 20964 |
KEGGi | mmu:20964 |
UCSCi | uc009stw.2, mouse [O88935-2] uc009stx.2, mouse [O88935-1] |
Organism-specific databases
CTDi | 6853 |
MGIi | MGI:98460, Syn1 |
Phylogenomic databases
eggNOGi | KOG3895, Eukaryota |
GeneTreei | ENSGT00940000161978 |
HOGENOMi | CLU_010582_3_0_1 |
InParanoidi | O88935 |
OMAi | APMTQGQ |
OrthoDBi | 799740at2759 |
PhylomeDBi | O88935 |
TreeFami | TF319919 |
Enzyme and pathway databases
Reactomei | R-MMU-181429, Serotonin Neurotransmitter Release Cycle R-MMU-212676, Dopamine Neurotransmitter Release Cycle |
Miscellaneous databases
BioGRID-ORCSi | 20964, 0 hits in 17 CRISPR screens |
ChiTaRSi | Syn1, mouse |
PROi | PR:O88935 |
RNActi | O88935, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037217, Expressed in hypothalamus and 147 other tissues |
Genevisiblei | O88935, MM |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR028713, SYN1 IPR001359, Synapsin IPR020898, Synapsin_ATP-bd_dom IPR019735, Synapsin_CS IPR019736, Synapsin_P_site IPR020897, Synapsin_pre-ATP-grasp_dom |
PANTHERi | PTHR10841:SF24, PTHR10841:SF24, 1 hit |
Pfami | View protein in Pfam PF02078, Synapsin, 1 hit PF02750, Synapsin_C, 1 hit PF10581, Synapsin_N, 1 hit |
PRINTSi | PR01368, SYNAPSIN |
SUPFAMi | SSF52440, SSF52440, 1 hit |
PROSITEi | View protein in PROSITE PS00415, SYNAPSIN_1, 1 hit PS00416, SYNAPSIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYN1_MOUSE | |
Accessioni | O88935Primary (citable) accession number: O88935 Secondary accession number(s): Q62279, Q8QZT8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | August 30, 2005 | |
Last modified: | December 2, 2020 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families