UniProtKB - O88908 (SOAT2_MOUSE)
Protein
Sterol O-acyltransferase 2
Gene
Soat2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA) as substrates. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.By similarity
Catalytic activityi
- EC:2.3.1.26By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z,17Z-eicosapentaenoate) + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z,12Z,15Z)-octadecatrienoyl-CoA + cholesterol = cholesteryl (9Z,12Z,15Z)-octadecatrienoate + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 118 | CholesterolBy similarity | 1 | |
Binding sitei | 392 | Acyl-CoABy similarity | 1 | |
Binding sitei | 395 | Acyl-CoABy similarity | 1 | |
Binding sitei | 398 | Acyl-CoABy similarity | 1 | |
Binding sitei | 402 | Acyl-CoABy similarity | 1 | |
Binding sitei | 410 | Acyl-CoABy similarity | 1 | |
Binding sitei | 433 | Acyl-CoABy similarity | 1 | |
Active sitei | 437 | By similarity | 1 |
GO - Molecular functioni
- cholesterol binding Source: MGI
- cholesterol O-acyltransferase activity Source: MGI
- fatty-acyl-CoA binding Source: MGI
- O-acyltransferase activity Source: GO_Central
- sterol O-acyltransferase activity Source: MGI
GO - Biological processi
- cholesterol efflux Source: MGI
- cholesterol esterification Source: UniProtKB
- cholesterol homeostasis Source: InterPro
- cholesterol metabolic process Source: MGI
- very-low-density lipoprotein particle assembly Source: MGI
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.26, 3474 |
Reactomei | R-MMU-8964038, LDL clearance |
Names & Taxonomyi
Protein namesi | Recommended name: Sterol O-acyltransferase 2Curated (EC:2.3.1.26By similarity)Alternative name(s): Acyl-coenzyme A:cholesterol acyltransferase 2 Short name: ACAT-2 Cholesterol acyltransferase 2 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1332226, Soat2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: MGI
Other locations
- brush border Source: MGI
- integral component of membrane Source: UniProtKB-KW
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 119 | CytoplasmicCuratedAdd BLAST | 119 | |
Transmembranei | 120 – 141 | Helical; Name=1By similarityAdd BLAST | 22 | |
Topological domaini | 142 – 161 | LumenalCuratedAdd BLAST | 20 | |
Transmembranei | 162 – 187 | Helical; Name=2By similarityAdd BLAST | 26 | |
Topological domaini | 188 – 199 | CytoplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 200 – 223 | Helical; Name=3By similarityAdd BLAST | 24 | |
Topological domaini | 224 – 231 | LumenalCurated | 8 | |
Transmembranei | 232 – 255 | Helical; Name=4By similarityAdd BLAST | 24 | |
Topological domaini | 256 – 296 | CytoplasmicCuratedAdd BLAST | 41 | |
Transmembranei | 297 – 329 | Helical; Name=5By similarityAdd BLAST | 33 | |
Topological domaini | 330 – 346 | LumenalCuratedAdd BLAST | 17 | |
Transmembranei | 347 – 372 | Helical; Name=6By similarityAdd BLAST | 26 | |
Topological domaini | 373 – 420 | CytoplasmicCuratedAdd BLAST | 48 | |
Transmembranei | 421 – 445 | Helical; Name=7By similarityAdd BLAST | 25 | |
Topological domaini | 446 – 451 | LumenalCurated | 6 | |
Transmembranei | 452 – 467 | Helical; Name=8By similarityAdd BLAST | 16 | |
Topological domaini | 468 – 473 | CytoplasmicCurated | 6 | |
Transmembranei | 474 – 505 | Helical; Name=9By similarityAdd BLAST | 32 | |
Topological domaini | 506 – 525 | LumenalCuratedAdd BLAST | 20 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Mice are more susceptible to high-fat diet-induced insulin resistance.1 Publication
Chemistry databases
ChEMBLi | CHEMBL2653 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207646 | 1 – 525 | Sterol O-acyltransferase 2Add BLAST | 525 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 280 | Cysteine sulfenic acid (-SOH); alternateBy similarity | 1 | |
Cross-linki | 280 | Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity |
Post-translational modificationi
Polyubiquitinated by AMFR/gp78 at Cys-280, leading to its degradation when the lipid levels are low. Association with AMFR/gp78 is mediated via interaction with INSIG1. High concentration of cholesterol and fatty acid results in Cys-280 oxidation, preventing ubiquitination at the same site, resulting in protein stabilization.By similarity
Oxidized at Cys-280: high concentration of cholesterol and fatty acid induce reactive oxygen species, which oxidizes Cys-280, preventing ubiquitination at the same site, and resulting in protein stabilization.By similarity
Keywords - PTMi
Oxidation, Thioester bond, Ubl conjugationProteomic databases
EPDi | O88908 |
jPOSTi | O88908 |
MaxQBi | O88908 |
PaxDbi | O88908 |
PRIDEi | O88908 |
PTM databases
iPTMneti | O88908 |
PhosphoSitePlusi | O88908 |
SwissPalmi | O88908 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000023045, Expressed in jejunum and 80 other tissues |
ExpressionAtlasi | O88908, baseline and differential |
Genevisiblei | O88908, MM |
Interactioni
Subunit structurei
May form homo- or heterodimers (By similarity).
Interacts with INSIG1; the interaction is direct and promotes association with AMFR/gp78 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000023806 |
Miscellaneous databases
RNActi | O88908, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 380 – 386 | FYXDWWN motifBy similarity | 7 |
Domaini
Each protomer consists of 9 transmembrane segments, which enclose a cytosolic tunnel and a transmembrane tunnel that converge at the predicted catalytic site: acyl-CoA enters the active site through the cytosolic tunnel, whereas cholesterol enters from the side through the transmembrane tunnel.By similarity
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0380, Eukaryota |
GeneTreei | ENSGT00950000183081 |
HOGENOMi | CLU_031845_1_0_1 |
InParanoidi | O88908 |
OMAi | TMNDRHT |
OrthoDBi | 1275897at2759 |
TreeFami | TF315226 |
Family and domain databases
InterProi | View protein in InterPro IPR004299, MBOAT_fam IPR014371, Oat_ACAT_DAG_ARE IPR030687, Sterol_acyltranf_meta |
PANTHERi | PTHR10408, PTHR10408, 1 hit |
Pfami | View protein in Pfam PF03062, MBOAT, 1 hit |
PIRSFi | PIRSF000439, Oat_ACAT_DAG_ARE, 1 hit PIRSF500230, Sterol_acyltranf_ACAT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O88908-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQPKVPQLRR REGLGEEQEK GARGGEGNAR THGTPDLVQW TRHMEAVKTQ
60 70 80 90 100
FLEQAQRELA ELLDRALWEA MQAYPKQDRP LPSAAPDSTS KTQELHPGKR
110 120 130 140 150
KVFITRKSLI DELMEVQHFR TIYHMFIAGL CVLIISTLAI DFIDEGRLML
160 170 180 190 200
EFDLLLFSFG QLPLALMTWV PMFLSTLLVP YQTLWLWARP RAGGAWMLGA
210 220 230 240 250
SLGCVLLAAH AVVLCVLPVH VSVRHELPPA SRCVLVFEQV RLLMKSYSFL
260 270 280 290 300
RETVPGIFCV RGGKGISPPS FSSYLYFLFC PTLIYRETYP RTPSIRWNYV
310 320 330 340 350
AKNFAQVLGC LLYACFILGR LCVPVFANMS REPFSTRALL LSILHATGPG
360 370 380 390 400
IFMLLLIFFA FLHCWLNAFA EMLRFGDRMF YRDWWNSTSF SNYYRTWNVV
410 420 430 440 450
VHDWLYSYVY QDGLWLLGRR ARGVAMLGVF LVSAVVHEYI FCFVLGFFYP
460 470 480 490 500
VMLMLFLVFG GLLNFTMNDR HTGPAWNILM WTFLFMGQGI QVSLYCQEWY
510 520
ARRHCPLPQT TFWGMVTPRS WSCHP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF6SMH4 | F6SMH4_MOUSE | Sterol O-acyltransferase 2 | Soat2 | 284 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 93 | Q → P in AAC64057 (PubMed:9756919).Curated | 1 | |
Sequence conflicti | 96 | H → R in AAC64057 (PubMed:9756919).Curated | 1 | |
Sequence conflicti | 104 – 105 | IT → VA in AAC64057 (PubMed:9756919).Curated | 2 | |
Sequence conflicti | 131 – 132 | CV → WF in AAC64057 (PubMed:9756919).Curated | 2 | |
Sequence conflicti | 175 | S → Y in AAC64057 (PubMed:9756919).Curated | 1 | |
Sequence conflicti | 262 | G → R in AAC64057 (PubMed:9756919).Curated | 1 | |
Sequence conflicti | 519 | R → Q in AAC64057 (PubMed:9756919).Curated | 1 | |
Sequence conflicti | 525 | P → T in AAC64057 (PubMed:9756919).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF078751 mRNA Translation: AAC64057.1 CH466550 Genomic DNA Translation: EDL04004.1 BC025931 mRNA Translation: AAH25931.1 |
CCDSi | CCDS27872.1 |
RefSeqi | NP_666176.1, NM_146064.1 |
Genome annotation databases
Ensembli | ENSMUST00000023806; ENSMUSP00000023806; ENSMUSG00000023045 |
GeneIDi | 223920 |
KEGGi | mmu:223920 |
UCSCi | uc007xut.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF078751 mRNA Translation: AAC64057.1 CH466550 Genomic DNA Translation: EDL04004.1 BC025931 mRNA Translation: AAH25931.1 |
CCDSi | CCDS27872.1 |
RefSeqi | NP_666176.1, NM_146064.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000023806 |
Chemistry databases
ChEMBLi | CHEMBL2653 |
PTM databases
iPTMneti | O88908 |
PhosphoSitePlusi | O88908 |
SwissPalmi | O88908 |
Proteomic databases
EPDi | O88908 |
jPOSTi | O88908 |
MaxQBi | O88908 |
PaxDbi | O88908 |
PRIDEi | O88908 |
Protocols and materials databases
Antibodypediai | 43294, 137 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000023806; ENSMUSP00000023806; ENSMUSG00000023045 |
GeneIDi | 223920 |
KEGGi | mmu:223920 |
UCSCi | uc007xut.1, mouse |
Organism-specific databases
CTDi | 8435 |
MGIi | MGI:1332226, Soat2 |
Phylogenomic databases
eggNOGi | KOG0380, Eukaryota |
GeneTreei | ENSGT00950000183081 |
HOGENOMi | CLU_031845_1_0_1 |
InParanoidi | O88908 |
OMAi | TMNDRHT |
OrthoDBi | 1275897at2759 |
TreeFami | TF315226 |
Enzyme and pathway databases
BRENDAi | 2.3.1.26, 3474 |
Reactomei | R-MMU-8964038, LDL clearance |
Miscellaneous databases
BioGRID-ORCSi | 223920, 4 hits in 17 CRISPR screens |
ChiTaRSi | Soat2, mouse |
PROi | PR:O88908 |
RNActi | O88908, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023045, Expressed in jejunum and 80 other tissues |
ExpressionAtlasi | O88908, baseline and differential |
Genevisiblei | O88908, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004299, MBOAT_fam IPR014371, Oat_ACAT_DAG_ARE IPR030687, Sterol_acyltranf_meta |
PANTHERi | PTHR10408, PTHR10408, 1 hit |
Pfami | View protein in Pfam PF03062, MBOAT, 1 hit |
PIRSFi | PIRSF000439, Oat_ACAT_DAG_ARE, 1 hit PIRSF500230, Sterol_acyltranf_ACAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SOAT2_MOUSE | |
Accessioni | O88908Primary (citable) accession number: O88908 Secondary accession number(s): Q8R0Y9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families