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Protein

Tumor protein 63

Gene

Tp63

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Activates transcription of the p21 promoter (By similarity). Activates RIPK4 transcription. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.By similarity5 Publications

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi244ZincBy similarity1
Metal bindingi247ZincBy similarity1
Metal bindingi308ZincBy similarity1
Metal bindingi312ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi170 – 362By similarityAdd BLAST193

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processApoptosis, Notch signaling pathway, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6804759 Regulation of TP53 Activity through Association with Co-factors

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor protein 63
Short name:
p63
Alternative name(s):
Transformation-related protein 63
Short name:
TP63
Tumor protein p73-like
Short name:
p73L
Gene namesi
Name:Tp63
Synonyms:P63, P73l, Tp73l, Trp63
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1330810 Trp63

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi543Y → F: Abolishes interaction with WWP1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857301 – 680Tumor protein 63Add BLAST680

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki676Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

May be sumoylated.By similarity
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO88898
PRIDEiO88898

PTM databases

iPTMnetiO88898
PhosphoSitePlusiO88898

Expressioni

Tissue specificityi

Widely expressed, notably in thymus, prostate, placenta and skeletal muscle, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast and prostate express high levels of DeltaN-type isoforms.1 Publication

Developmental stagei

TA*-type isoforms are expressed from E7.5, prior to the onset of epithelial stratification, while DeltaN-type isoforms are expressed from E9.5.1 Publication

Inductioni

Induced by DNA damaging agents.1 Publication

Gene expression databases

BgeeiENSMUSG00000022510 Expressed in 187 organ(s), highest expression level in hair follicle
CleanExiMM_TRP63
ExpressionAtlasiO88898 baseline and differential
GenevisibleiO88898 MM

Interactioni

Subunit structurei

Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Interacts with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Interacts with PDS5A. Interacts (via activation domain) with NOC2L (By similarity). Interacts with WWP1.By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204325, 3 interactors
ELMiO88898
IntActiO88898, 7 interactors
MINTiO88898
STRINGi10090.ENSMUSP00000110965

Structurei

Secondary structure

1680
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO88898
SMRiO88898
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini541 – 607SAMAdd BLAST67

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 107Transcription activationBy similarityAdd BLAST107
Regioni352 – 388Interaction with HIPK2By similarityAdd BLAST37
Regioni394 – 443OligomerizationBy similarityAdd BLAST50
Regioni610 – 680Transactivation inhibitionBy similarityAdd BLAST71

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi437 – 444Poly-Gln8

Domaini

The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.By similarity

Sequence similaritiesi

Belongs to the p53 family.Curated

Phylogenomic databases

eggNOGiENOG410IGE4 Eukaryota
ENOG410XV9W LUCA
GeneTreeiENSGT00390000015092
HOVERGENiHBG005201
InParanoidiO88898
KOiK10149
OMAiSKMNSMN
OrthoDBiEOG091G0XY5
PhylomeDBiO88898
TreeFamiTF106101

Family and domain databases

CDDicd08367 P53, 1 hit
cd09572 SAM_tumor-p63, 1 hit
Gene3Di2.60.40.720, 1 hit
4.10.170.10, 1 hit
InterProiView protein in InterPro
IPR008967 p53-like_TF_DNA-bd
IPR012346 p53/RUNT-type_TF_DNA-bd_sf
IPR011615 p53_DNA-bd
IPR036674 p53_tetramer_sf
IPR010991 p53_tetrameristn
IPR002117 p53_tumour_suppressor
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR032645 Tp63
IPR037611 Tumor-p63_SAM
PANTHERiPTHR11447 PTHR11447, 1 hit
PTHR11447:SF8 PTHR11447:SF8, 1 hit
PfamiView protein in Pfam
PF00870 P53, 1 hit
PF07710 P53_tetramer, 1 hit
PF07647 SAM_2, 1 hit
PRINTSiPR00386 P53SUPPRESSR
SMARTiView protein in SMART
SM00454 SAM, 1 hit
SUPFAMiSSF47719 SSF47719, 1 hit
SSF47769 SSF47769, 1 hit
SSF49417 SSF49417, 1 hit
PROSITEiView protein in PROSITE
PS00348 P53, 1 hit

Sequences (6+)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O88898-1) [UniParc]FASTAAdd to basket
Also known as: TA*-alpha, TA*p63alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNFETSRCAT LQYCPDPYIQ RFIETPAHFS WKESYYRSAM SQSTQTSEFL
60 70 80 90 100
SPEVFQHIWD FLEQPICSVQ PIELNFVDEP SENGATNKIE ISMDCIRMQD
110 120 130 140 150
SDLSDPMWPQ YTNLGLLNSM DQQIQNGSSS TSPYNTDHAQ NSVTAPSPYA
160 170 180 190 200
QPSSTFDALS PSPAIPSNTD YPGPHSFDVS FQQSSTAKSA TWTYSTELKK
210 220 230 240 250
LYCQIAKTCP IQIKVMTPPP QGAVIRAMPV YKKAEHVTEV VKRCPNHELS
260 270 280 290 300
REFNEGQIAP PSHLIRVEGN SHAQYVEDPI TGRQSVLVPY EPPQVGTEFT
310 320 330 340 350
TVLYNFMCNS SCVGGMNRRP ILIIVTLETR DGQVLGRRCF EARICACPGR
360 370 380 390 400
DRKADEDSIR KQQVSDSAKN GDGTKRPFRQ NTHGIQMTSI KKRRSPDDEL
410 420 430 440 450
LYLPVRGRET YEMLLKIKES LELMQYLPQH TIETYRQQQQ QQHQHLLQKQ
460 470 480 490 500
TSMQSQSSYG NSSPPLNKMN SMNKLPSVSQ LINPQQRNAL TPTTMPEGMG
510 520 530 540 550
ANIPMMGTHM PMAGDMNGLS PTQALPPPLS MPSTSHCTPP PPYPTDCSIV
560 570 580 590 600
SFLARLGCSS CLDYFTTQGL TTIYQIEHYS MDDLASLKIP EQFRHAIWKG
610 620 630 640 650
ILDHRQLHDF SSPPHLLRTP SGASTVSVGS SETRGERVID AVRFTLRQTI
660 670 680
SFPPRDEWND FNFDMDSRRN KQQRIKEEGE
Note: Produced by alternative promoter usage.
Length:680
Mass (Da):76,789
Last modified:November 1, 1998 - v1
Checksum:i8DFF0284F247C68A
GO
Isoform 2 (identifier: O88898-2) [UniParc]FASTAAdd to basket
Also known as: DeltaN-alpha

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: MNFETSRCAT...QDSDLSDPMW → MLYLENNAQTQFSE

Note: Produced by alternative promoter usage.
Show »
Length:586
Mass (Da):65,789
Checksum:i622E24085B8BDCB7
GO
Isoform 3 (identifier: O88898-3) [UniParc]FASTAAdd to basket
Also known as: TA*-beta, TA*p63beta

The sequence of this isoform differs from the canonical sequence as follows:
     551-680: SFLARLGCSS...KQQRIKEEGE → RIWQV

Note: Produced by alternative splicing of isoform 1.
Show »
Length:555
Mass (Da):62,455
Checksum:i059E034046EB8987
GO
Isoform 4 (identifier: O88898-4) [UniParc]FASTAAdd to basket
Also known as: DeltaN-beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: MNFETSRCAT...QDSDLSDPMW → MLYLENNAQTQFSE
     551-680: SFLARLGCSS...KQQRIKEEGE → RIWQV

Note: Produced by alternative splicing of isoform 2.
Show »
Length:461
Mass (Da):51,455
Checksum:i7613296F2F85DBC8
GO
Isoform 5 (identifier: O88898-5) [UniParc]FASTAAdd to basket
Also known as: TA*-gamma, TA*p63gamma

The sequence of this isoform differs from the canonical sequence as follows:
     373-377: GTKRP → A
     450-680: QTSMQSQSSY...KQQRIKEEGE → HLLSACFRNE...SNPPNHSVYP

Note: Produced by alternative splicing of isoform 1.
Show »
Length:483
Mass (Da):54,970
Checksum:iA90ED0C110C50EAD
GO
Isoform 6 (identifier: O88898-6) [UniParc]FASTAAdd to basket
Also known as: DeltaN-gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: MNFETSRCAT...QDSDLSDPMW → MLYLENNAQTQFSE
     373-377: GTKRP → A
     450-680: QTSMQSQSSY...KQQRIKEEGE → HLLSACFRNE...SNPPNHSVYP

Note: Produced by alternative splicing of isoform 2.
Show »
Length:389
Mass (Da):43,970
Checksum:iFE6B4A859C5F00BB
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q569E5Q569E5_MOUSE
Cellular tumor antigen p53
Trp63
393Annotation score:
A0A0R4J1K8A0A0R4J1K8_MOUSE
Cellular tumor antigen p53
Trp63
284Annotation score:
Q3UVI3Q3UVI3_MOUSE
Transformation-related protein 63
Trp63
582Annotation score:
D3Z7K2D3Z7K2_MOUSE
Transformation-related protein 63
Trp63
231Annotation score:

Sequence cautioni

The sequence BAC33397 differs from that shown. Intron retention.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0124711 – 108MNFET…SDPMW → MLYLENNAQTQFSE in isoform 2, isoform 4 and isoform 6. 3 PublicationsAdd BLAST108
Alternative sequenceiVSP_012472373 – 377GTKRP → A in isoform 5 and isoform 6. 1 Publication5
Alternative sequenceiVSP_012473450 – 680QTSMQ…KEEGE → HLLSACFRNELVEPRGEAPT QSDVFFRHSNPPNHSVYP in isoform 5 and isoform 6. 1 PublicationAdd BLAST231
Alternative sequenceiVSP_012474551 – 680SFLAR…KEEGE → RIWQV in isoform 3 and isoform 4. 2 PublicationsAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075434 mRNA Translation: AAC62639.1
AF075435 mRNA Translation: AAC62640.1
AF075436 mRNA Translation: AAC62641.1
AF075437 mRNA Translation: AAC62642.1
AF075438 mRNA Translation: AAC62643.1
AF075439 mRNA Translation: AAC62644.1
AB010152 mRNA Translation: BAA32432.1
AF533892 Genomic DNA Translation: AAP87982.1
AF533892 Genomic DNA Translation: AAP87985.1
AF533892 Genomic DNA Translation: AAP87983.1
AF533892 Genomic DNA Translation: AAP87984.1
AF533892 Genomic DNA Translation: AAP87986.1
AF533892 Genomic DNA Translation: AAP87987.1
AK048623 mRNA Translation: BAC33397.1 Sequence problems.
CCDSiCCDS28085.1 [O88898-2]
CCDS49808.1 [O88898-5]
CCDS49809.1 [O88898-1]
CCDS49810.1 [O88898-3]
RefSeqiNP_001120731.1, NM_001127259.1 [O88898-1]
NP_001120732.1, NM_001127260.1 [O88898-3]
NP_001120733.1, NM_001127261.1 [O88898-5]
NP_001120734.1, NM_001127262.1 [O88898-4]
NP_001120736.1, NM_001127264.1
NP_001120737.1, NM_001127265.1 [O88898-6]
NP_035771.1, NM_011641.2 [O88898-2]
UniGeneiMm.20894

Genome annotation databases

EnsembliENSMUST00000040231; ENSMUSP00000038117; ENSMUSG00000022510 [O88898-2]
ENSMUST00000065523; ENSMUSP00000067005; ENSMUSG00000022510 [O88898-3]
ENSMUST00000115308; ENSMUSP00000110963; ENSMUSG00000022510 [O88898-5]
ENSMUST00000115310; ENSMUSP00000110965; ENSMUSG00000022510 [O88898-1]
GeneIDi22061
KEGGimmu:22061
UCSCiuc007yuo.2 mouse [O88898-5]
uc007yup.2 mouse [O88898-1]
uc007yuq.2 mouse [O88898-3]
uc007yut.2 mouse [O88898-6]
uc007yuu.2 mouse [O88898-2]
uc007yuv.2 mouse [O88898-4]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075434 mRNA Translation: AAC62639.1
AF075435 mRNA Translation: AAC62640.1
AF075436 mRNA Translation: AAC62641.1
AF075437 mRNA Translation: AAC62642.1
AF075438 mRNA Translation: AAC62643.1
AF075439 mRNA Translation: AAC62644.1
AB010152 mRNA Translation: BAA32432.1
AF533892 Genomic DNA Translation: AAP87982.1
AF533892 Genomic DNA Translation: AAP87985.1
AF533892 Genomic DNA Translation: AAP87983.1
AF533892 Genomic DNA Translation: AAP87984.1
AF533892 Genomic DNA Translation: AAP87986.1
AF533892 Genomic DNA Translation: AAP87987.1
AK048623 mRNA Translation: BAC33397.1 Sequence problems.
CCDSiCCDS28085.1 [O88898-2]
CCDS49808.1 [O88898-5]
CCDS49809.1 [O88898-1]
CCDS49810.1 [O88898-3]
RefSeqiNP_001120731.1, NM_001127259.1 [O88898-1]
NP_001120732.1, NM_001127260.1 [O88898-3]
NP_001120733.1, NM_001127261.1 [O88898-5]
NP_001120734.1, NM_001127262.1 [O88898-4]
NP_001120736.1, NM_001127264.1
NP_001120737.1, NM_001127265.1 [O88898-6]
NP_035771.1, NM_011641.2 [O88898-2]
UniGeneiMm.20894

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5N2ONMR-A545-609[»]
ProteinModelPortaliO88898
SMRiO88898
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204325, 3 interactors
ELMiO88898
IntActiO88898, 7 interactors
MINTiO88898
STRINGi10090.ENSMUSP00000110965

PTM databases

iPTMnetiO88898
PhosphoSitePlusiO88898

Proteomic databases

PaxDbiO88898
PRIDEiO88898

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040231; ENSMUSP00000038117; ENSMUSG00000022510 [O88898-2]
ENSMUST00000065523; ENSMUSP00000067005; ENSMUSG00000022510 [O88898-3]
ENSMUST00000115308; ENSMUSP00000110963; ENSMUSG00000022510 [O88898-5]
ENSMUST00000115310; ENSMUSP00000110965; ENSMUSG00000022510 [O88898-1]
GeneIDi22061
KEGGimmu:22061
UCSCiuc007yuo.2 mouse [O88898-5]
uc007yup.2 mouse [O88898-1]
uc007yuq.2 mouse [O88898-3]
uc007yut.2 mouse [O88898-6]
uc007yuu.2 mouse [O88898-2]
uc007yuv.2 mouse [O88898-4]

Organism-specific databases

CTDi22061
MGIiMGI:1330810 Trp63

Phylogenomic databases

eggNOGiENOG410IGE4 Eukaryota
ENOG410XV9W LUCA
GeneTreeiENSGT00390000015092
HOVERGENiHBG005201
InParanoidiO88898
KOiK10149
OMAiSKMNSMN
OrthoDBiEOG091G0XY5
PhylomeDBiO88898
TreeFamiTF106101

Enzyme and pathway databases

ReactomeiR-MMU-6804759 Regulation of TP53 Activity through Association with Co-factors

Miscellaneous databases

ChiTaRSiTcp1 mouse
PROiPR:O88898
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022510 Expressed in 187 organ(s), highest expression level in hair follicle
CleanExiMM_TRP63
ExpressionAtlasiO88898 baseline and differential
GenevisibleiO88898 MM

Family and domain databases

CDDicd08367 P53, 1 hit
cd09572 SAM_tumor-p63, 1 hit
Gene3Di2.60.40.720, 1 hit
4.10.170.10, 1 hit
InterProiView protein in InterPro
IPR008967 p53-like_TF_DNA-bd
IPR012346 p53/RUNT-type_TF_DNA-bd_sf
IPR011615 p53_DNA-bd
IPR036674 p53_tetramer_sf
IPR010991 p53_tetrameristn
IPR002117 p53_tumour_suppressor
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR032645 Tp63
IPR037611 Tumor-p63_SAM
PANTHERiPTHR11447 PTHR11447, 1 hit
PTHR11447:SF8 PTHR11447:SF8, 1 hit
PfamiView protein in Pfam
PF00870 P53, 1 hit
PF07710 P53_tetramer, 1 hit
PF07647 SAM_2, 1 hit
PRINTSiPR00386 P53SUPPRESSR
SMARTiView protein in SMART
SM00454 SAM, 1 hit
SUPFAMiSSF47719 SSF47719, 1 hit
SSF47769 SSF47769, 1 hit
SSF49417 SSF49417, 1 hit
PROSITEiView protein in PROSITE
PS00348 P53, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiP63_MOUSE
AccessioniPrimary (citable) accession number: O88898
Secondary accession number(s): O88897
, O88899, O89097, Q8C826, Q9QWY9, Q9QWZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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