UniProtKB - O88898 (P63_MOUSE)
Tumor protein 63
Tp63
Functioni
Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Activates transcription of the p21 promoter (By similarity).
Activates RIPK4 transcription. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.
By similarity5 PublicationsCofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 244 | ZincBy similarity | 1 | |
Metal bindingi | 247 | ZincBy similarity | 1 | |
Metal bindingi | 308 | ZincBy similarity | 1 | |
Metal bindingi | 312 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 170 – 362 | By similarityAdd BLAST | 193 |
GO - Molecular functioni
- chromatin binding Source: MGI
- damaged DNA binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- double-stranded DNA binding Source: MGI
- identical protein binding Source: IntAct
- MDM2/MDM4 family protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- p53 binding Source: MGI
- protein domain specific binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- WW domain binding Source: MGI
GO - Biological processi
- anatomical structure formation involved in morphogenesis Source: MGI
- animal organ morphogenesis Source: MGI
- apoptotic process Source: MGI
- cell aging Source: MGI
- cell differentiation Source: MGI
- cell population proliferation Source: MGI
- cellular response to DNA damage stimulus Source: MGI
- chromatin remodeling Source: MGI
- cloacal septation Source: MGI
- cranial skeletal system development Source: MGI
- ectoderm and mesoderm interaction Source: MGI
- embryonic forelimb morphogenesis Source: MGI
- embryonic hindlimb morphogenesis Source: MGI
- embryonic limb morphogenesis Source: MGI
- epidermal cell differentiation Source: MGI
- epidermal cell division Source: MGI
- epidermis development Source: MGI
- epithelial cell development Source: MGI
- epithelial cell differentiation Source: MGI
- establishment of planar polarity Source: MGI
- establishment of skin barrier Source: UniProtKB
- female genitalia morphogenesis Source: MGI
- hair follicle development Source: MGI
- hair follicle morphogenesis Source: MGI
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
- keratinocyte differentiation Source: MGI
- morphogenesis of a polarized epithelium Source: MGI
- multicellular organism aging Source: MGI
- negative regulation of cellular senescence Source: MGI
- negative regulation of intracellular estrogen receptor signaling pathway Source: CAFA
- negative regulation of keratinocyte differentiation Source: MGI
- negative regulation of mesoderm development Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- neuron apoptotic process Source: MGI
- Notch signaling pathway Source: UniProtKB-KW
- odontogenesis of dentin-containing tooth Source: MGI
- pattern specification process Source: MGI
- polarized epithelial cell differentiation Source: MGI
- positive regulation of apoptotic signaling pathway Source: MGI
- positive regulation of cell cycle G1/S phase transition Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of fibroblast apoptotic process Source: MGI
- positive regulation of keratinocyte proliferation Source: MGI
- positive regulation of Notch signaling pathway Source: MGI
- positive regulation of osteoblast differentiation Source: MGI
- positive regulation of somatic stem cell population maintenance Source: CAFA
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- post-anal tail morphogenesis Source: MGI
- prostate gland development Source: MGI
- prostatic bud formation Source: MGI
- protein tetramerization Source: InterPro
- proximal/distal pattern formation Source: MGI
- regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- regulation of epidermal cell division Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- skeletal system development Source: MGI
- skin epidermis development Source: MGI
- skin morphogenesis Source: MGI
- spermatogenesis Source: Ensembl
- squamous basal epithelial stem cell differentiation involved in prostate gland acinus development Source: MGI
- sympathetic nervous system development Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Apoptosis, Notch signaling pathway, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-6804759, Regulation of TP53 Activity through Association with Co-factors |
Names & Taxonomyi
Protein namesi | Recommended name: Tumor protein 63Short name: p63 Alternative name(s): Transformation-related protein 63 Short name: TP63 Tumor protein p73-like Short name: p73L |
Gene namesi | Name:Tp63 Synonyms:P63, P73l, Tp73l, Trp63 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1330810, Trp63 |
VEuPathDBi | HostDB:ENSMUSG00000022510 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Endoplasmic reticulum
- rough endoplasmic reticulum Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
- cytoplasm Source: MGI
- dendrite Source: MGI
- neuron projection Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 543 | Y → F: Abolishes interaction with WWP1. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185730 | 1 – 680 | Tumor protein 63Add BLAST | 680 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 676 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | O88898 |
PRIDEi | O88898 |
PTM databases
iPTMneti | O88898 |
PhosphoSitePlusi | O88898 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000022510, Expressed in hair follicle and 214 other tissues |
ExpressionAtlasi | O88898, baseline and differential |
Genevisiblei | O88898, MM |
Interactioni
Subunit structurei
Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity.
Interacts with HIPK2.
Interacts with SSRP1, leading to stimulate coactivator activity.
Interacts with PDS5A.
Interacts (via activation domain) with NOC2L (By similarity).
Interacts with WWP1.
By similarity1 PublicationBinary interactionsi
O88898
With | #Exp. | IntAct |
---|---|---|
Cables1 [Q9ESJ1] | 2 | EBI-2338025,EBI-604411 |
itself | 2 | EBI-2338025,EBI-2338025 |
TP53 [P04637] from Homo sapiens. | 2 | EBI-2338025,EBI-366083 |
Tp63 - isoform 1 [O88898-1]
With | #Exp. | IntAct |
---|---|---|
itself | 19 | EBI-3863990,EBI-3863990 |
Tp63 - isoform 2 [O88898-2]
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-2338228,EBI-2338228 |
KAT2B [Q92831] from Homo sapiens. | 3 | EBI-2338228,EBI-477430 |
Tp63 - isoform 5 [O88898-5]
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-2338240,EBI-2338240 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- MDM2/MDM4 family protein binding Source: MGI
- p53 binding Source: MGI
- protein domain specific binding Source: MGI
- WW domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204325, 8 interactors |
ELMi | O88898 |
IntActi | O88898, 7 interactors |
MINTi | O88898 |
STRINGi | 10090.ENSMUSP00000110965 |
Miscellaneous databases
RNActi | O88898, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O88898 |
BMRBi | O88898 |
SMRi | O88898 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 541 – 607 | SAMAdd BLAST | 67 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 107 | Transcription activationBy similarityAdd BLAST | 107 | |
Regioni | 123 – 171 | DisorderedSequence analysisAdd BLAST | 49 | |
Regioni | 351 – 393 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 352 – 388 | Interaction with HIPK2By similarityAdd BLAST | 37 | |
Regioni | 394 – 443 | OligomerizationBy similarityAdd BLAST | 50 | |
Regioni | 436 – 472 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 610 – 680 | Transactivation inhibitionBy similarityAdd BLAST | 71 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 123 – 166 | Polar residuesSequence analysisAdd BLAST | 44 | |
Compositional biasi | 364 – 389 | Polar residuesSequence analysisAdd BLAST | 26 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QQ48, Eukaryota |
GeneTreei | ENSGT00950000183153 |
HOGENOMi | CLU_019621_1_0_1 |
InParanoidi | O88898 |
OMAi | IRMQDSE |
PhylomeDBi | O88898 |
TreeFami | TF106101 |
Family and domain databases
CDDi | cd08367, P53, 1 hit cd09572, SAM_tumor-p63, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 2.60.40.720, 1 hit 4.10.170.10, 1 hit |
InterProi | View protein in InterPro IPR008967, p53-like_TF_DNA-bd IPR012346, p53/RUNT-type_TF_DNA-bd_sf IPR011615, p53_DNA-bd IPR036674, p53_tetramer_sf IPR010991, p53_tetrameristn IPR002117, p53_tumour_suppressor IPR001660, SAM IPR013761, SAM/pointed_sf IPR032645, Tp63 IPR037611, Tumor-p63_SAM |
PANTHERi | PTHR11447, PTHR11447, 1 hit PTHR11447:SF8, PTHR11447:SF8, 1 hit |
Pfami | View protein in Pfam PF00870, P53, 1 hit PF07710, P53_tetramer, 1 hit PF07647, SAM_2, 1 hit |
PRINTSi | PR00386, P53SUPPRESSR |
SMARTi | View protein in SMART SM00454, SAM, 1 hit |
SUPFAMi | SSF47719, SSF47719, 1 hit SSF47769, SSF47769, 1 hit SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS00348, P53, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative promoter usage and alternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNFETSRCAT LQYCPDPYIQ RFIETPAHFS WKESYYRSAM SQSTQTSEFL
60 70 80 90 100
SPEVFQHIWD FLEQPICSVQ PIELNFVDEP SENGATNKIE ISMDCIRMQD
110 120 130 140 150
SDLSDPMWPQ YTNLGLLNSM DQQIQNGSSS TSPYNTDHAQ NSVTAPSPYA
160 170 180 190 200
QPSSTFDALS PSPAIPSNTD YPGPHSFDVS FQQSSTAKSA TWTYSTELKK
210 220 230 240 250
LYCQIAKTCP IQIKVMTPPP QGAVIRAMPV YKKAEHVTEV VKRCPNHELS
260 270 280 290 300
REFNEGQIAP PSHLIRVEGN SHAQYVEDPI TGRQSVLVPY EPPQVGTEFT
310 320 330 340 350
TVLYNFMCNS SCVGGMNRRP ILIIVTLETR DGQVLGRRCF EARICACPGR
360 370 380 390 400
DRKADEDSIR KQQVSDSAKN GDGTKRPFRQ NTHGIQMTSI KKRRSPDDEL
410 420 430 440 450
LYLPVRGRET YEMLLKIKES LELMQYLPQH TIETYRQQQQ QQHQHLLQKQ
460 470 480 490 500
TSMQSQSSYG NSSPPLNKMN SMNKLPSVSQ LINPQQRNAL TPTTMPEGMG
510 520 530 540 550
ANIPMMGTHM PMAGDMNGLS PTQALPPPLS MPSTSHCTPP PPYPTDCSIV
560 570 580 590 600
SFLARLGCSS CLDYFTTQGL TTIYQIEHYS MDDLASLKIP EQFRHAIWKG
610 620 630 640 650
ILDHRQLHDF SSPPHLLRTP SGASTVSVGS SETRGERVID AVRFTLRQTI
660 670 680
SFPPRDEWND FNFDMDSRRN KQQRIKEEGE
The sequence of this isoform differs from the canonical sequence as follows:
1-108: MNFETSRCAT...QDSDLSDPMW → MLYLENNAQTQFSE
551-680: SFLARLGCSS...KQQRIKEEGE → RIWQV
The sequence of this isoform differs from the canonical sequence as follows:
373-377: GTKRP → A
450-680: QTSMQSQSSY...KQQRIKEEGE → HLLSACFRNE...SNPPNHSVYP
The sequence of this isoform differs from the canonical sequence as follows:
1-108: MNFETSRCAT...QDSDLSDPMW → MLYLENNAQTQFSE
373-377: GTKRP → A
450-680: QTSMQSQSSY...KQQRIKEEGE → HLLSACFRNE...SNPPNHSVYP
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ569E5 | Q569E5_MOUSE | Cellular tumor antigen p53 | Trp63 | 393 | Annotation score: | ||
Q3UVI3 | Q3UVI3_MOUSE | Tumor protein 63 (p63) | Trp63 | 582 | Annotation score: | ||
A0A0R4J1K8 | A0A0R4J1K8_MOUSE | Cellular tumor antigen p53 | Trp63 | 284 | Annotation score: | ||
D3Z7K2 | D3Z7K2_MOUSE | Transformation-related protein 63 | Trp63 | 231 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012471 | 1 – 108 | MNFET…SDPMW → MLYLENNAQTQFSE in isoform 2, isoform 4 and isoform 6. 3 PublicationsAdd BLAST | 108 | |
Alternative sequenceiVSP_012472 | 373 – 377 | GTKRP → A in isoform 5 and isoform 6. 1 Publication | 5 | |
Alternative sequenceiVSP_012473 | 450 – 680 | QTSMQ…KEEGE → HLLSACFRNELVEPRGEAPT QSDVFFRHSNPPNHSVYP in isoform 5 and isoform 6. 1 PublicationAdd BLAST | 231 | |
Alternative sequenceiVSP_012474 | 551 – 680 | SFLAR…KEEGE → RIWQV in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 130 |
Sequence databases
Genome annotation databases
Ensembli | ENSMUST00000040231; ENSMUSP00000038117; ENSMUSG00000022510 [O88898-2] ENSMUST00000065523; ENSMUSP00000067005; ENSMUSG00000022510 [O88898-3] ENSMUST00000115308; ENSMUSP00000110963; ENSMUSG00000022510 [O88898-5] ENSMUST00000115310; ENSMUSP00000110965; ENSMUSG00000022510 [O88898-1] |
GeneIDi | 22061 |
KEGGi | mmu:22061 |
UCSCi | uc007yuo.2, mouse [O88898-5] uc007yup.2, mouse [O88898-1] uc007yuq.2, mouse [O88898-3] uc007yut.2, mouse [O88898-6] uc007yuu.2, mouse [O88898-2] uc007yuv.2, mouse [O88898-4] |
Keywords - Coding sequence diversityi
Alternative promoter usage, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5N2O | NMR | - | A | 545-609 | [»] | |
AlphaFoldDBi | O88898 | |||||
BMRBi | O88898 | |||||
SMRi | O88898 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204325, 8 interactors |
ELMi | O88898 |
IntActi | O88898, 7 interactors |
MINTi | O88898 |
STRINGi | 10090.ENSMUSP00000110965 |
PTM databases
iPTMneti | O88898 |
PhosphoSitePlusi | O88898 |
Proteomic databases
PaxDbi | O88898 |
PRIDEi | O88898 |
Protocols and materials databases
Antibodypediai | 1750, 1033 antibodies from 50 providers |
DNASUi | 22061 |
Genome annotation databases
Ensembli | ENSMUST00000040231; ENSMUSP00000038117; ENSMUSG00000022510 [O88898-2] ENSMUST00000065523; ENSMUSP00000067005; ENSMUSG00000022510 [O88898-3] ENSMUST00000115308; ENSMUSP00000110963; ENSMUSG00000022510 [O88898-5] ENSMUST00000115310; ENSMUSP00000110965; ENSMUSG00000022510 [O88898-1] |
GeneIDi | 22061 |
KEGGi | mmu:22061 |
UCSCi | uc007yuo.2, mouse [O88898-5] uc007yup.2, mouse [O88898-1] uc007yuq.2, mouse [O88898-3] uc007yut.2, mouse [O88898-6] uc007yuu.2, mouse [O88898-2] uc007yuv.2, mouse [O88898-4] |
Organism-specific databases
CTDi | 22061 |
MGIi | MGI:1330810, Trp63 |
VEuPathDBi | HostDB:ENSMUSG00000022510 |
Phylogenomic databases
eggNOGi | ENOG502QQ48, Eukaryota |
GeneTreei | ENSGT00950000183153 |
HOGENOMi | CLU_019621_1_0_1 |
InParanoidi | O88898 |
OMAi | IRMQDSE |
PhylomeDBi | O88898 |
TreeFami | TF106101 |
Enzyme and pathway databases
Reactomei | R-MMU-6804759, Regulation of TP53 Activity through Association with Co-factors |
Miscellaneous databases
BioGRID-ORCSi | 22061, 1 hit in 73 CRISPR screens |
ChiTaRSi | Trp63, mouse |
PROi | PR:O88898 |
RNActi | O88898, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022510, Expressed in hair follicle and 214 other tissues |
ExpressionAtlasi | O88898, baseline and differential |
Genevisiblei | O88898, MM |
Family and domain databases
CDDi | cd08367, P53, 1 hit cd09572, SAM_tumor-p63, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 2.60.40.720, 1 hit 4.10.170.10, 1 hit |
InterProi | View protein in InterPro IPR008967, p53-like_TF_DNA-bd IPR012346, p53/RUNT-type_TF_DNA-bd_sf IPR011615, p53_DNA-bd IPR036674, p53_tetramer_sf IPR010991, p53_tetrameristn IPR002117, p53_tumour_suppressor IPR001660, SAM IPR013761, SAM/pointed_sf IPR032645, Tp63 IPR037611, Tumor-p63_SAM |
PANTHERi | PTHR11447, PTHR11447, 1 hit PTHR11447:SF8, PTHR11447:SF8, 1 hit |
Pfami | View protein in Pfam PF00870, P53, 1 hit PF07710, P53_tetramer, 1 hit PF07647, SAM_2, 1 hit |
PRINTSi | PR00386, P53SUPPRESSR |
SMARTi | View protein in SMART SM00454, SAM, 1 hit |
SUPFAMi | SSF47719, SSF47719, 1 hit SSF47769, SSF47769, 1 hit SSF49417, SSF49417, 1 hit |
PROSITEi | View protein in PROSITE PS00348, P53, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | P63_MOUSE | |
Accessioni | O88898Primary (citable) accession number: O88898 Secondary accession number(s): O88897 Q9QWZ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families