Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Apoptotic protease-activating factor 1

Gene

Apaf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).By similarity

Miscellaneous

Physiological concentrations of calcium ions negatively affect the assembly of apoptosome by inhibiting nucleotide exchange in the monomeric form.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi154 – 161ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis
LigandATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptotic protease-activating factor 1
Short name:
APAF-1
Gene namesi
Name:Apaf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1306796 Apaf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi265R → S: No stimulation of CASP9 activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508451 – 1249Apoptotic protease-activating factor 1Add BLAST1249

Proteomic databases

EPDiO88879
MaxQBiO88879
PaxDbiO88879
PeptideAtlasiO88879
PRIDEiO88879

PTM databases

iPTMnetiO88879
PhosphoSitePlusiO88879

Expressioni

Tissue specificityi

Highly expressed in lung and spleen, weakly in brain and kidney and not detectable in liver.

Developmental stagei

High levels in embryonic brain and liver from E11.5 to E17.5 day.

Gene expression databases

BgeeiENSMUSG00000019979 Expressed in 266 organ(s), highest expression level in epithelium of small intestine
CleanExiMM_APAF1
ExpressionAtlasiO88879 baseline and differential
GenevisibleiO88879 MM

Interactioni

Subunit structurei

Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex (By similarity). Interacts with UACA. It may also interact with Bcl-XL. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain) (By similarity). Interacts with CIAO2A (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198139, 1 interactor
CORUMiO88879
IntActiO88879, 1 interactor
STRINGi10090.ENSMUSP00000020157

Structurei

Secondary structure

11249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO88879
SMRiO88879
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 90CARDPROSITE-ProRule annotationAdd BLAST90
Domaini106 – 415NB-ARCAdd BLAST310
Repeati613 – 652WD 1-1PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati655 – 694WD 1-2PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati697 – 738WD 1-3PROSITE-ProRule annotation1 PublicationAdd BLAST42
Repeati741 – 780WD 1-4PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati796 – 837WD 1-5PROSITE-ProRule annotation1 PublicationAdd BLAST42
Repeati838 – 877WD 1-6PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati880 – 910WD 1-7PROSITE-ProRule annotation1 PublicationAdd BLAST31
Repeati922 – 958WD 2-1PROSITE-ProRule annotation1 PublicationAdd BLAST37
Repeati959 – 998WD 2-2PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati1001 – 1040WD 2-3PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati1042 – 1080WD 2-4PROSITE-ProRule annotation1 PublicationAdd BLAST39
Repeati1083 – 1122WD 2-5PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati1125 – 1164WD 2-6PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati1176 – 1213WD 2-7PROSITE-ProRule annotation1 PublicationAdd BLAST38
Repeati1214 – 1249WD 2-8PROSITE-ProRule annotation1 PublicationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni910 – 921Interpropeller linkerAdd BLAST12

Domaini

The CARD domain mediates interaction with APIP.1 Publication
The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization.1 Publication

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4155 Eukaryota
KOG4658 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOVERGENiHBG018730
InParanoidiO88879
KOiK02084
OMAiSRIGHKK
OrthoDBiEOG091G036U
PhylomeDBiO88879
TreeFamiTF323866

Family and domain databases

CDDicd08323 CARD_APAF1, 1 hit
Gene3Di1.10.10.10, 1 hit
2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR017251 Apaf-1
IPR037963 APAF1_CARD_dom
IPR024977 Apc4_WD40_dom
IPR001315 CARD
IPR011029 DEATH-like_dom_sf
IPR020472 G-protein_beta_WD-40_rep
IPR002182 NB-ARC
IPR027417 P-loop_NTPase
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 1 hit
PF00619 CARD, 1 hit
PF00931 NB-ARC, 1 hit
PF00400 WD40, 8 hits
PIRSFiPIRSF037646 Apop_pept_activating-1, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 13 hits
SUPFAMiSSF47986 SSF47986, 1 hit
SSF50978 SSF50978, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50209 CARD, 1 hit
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 9 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O88879-1) [UniParc]FASTAAdd to basket
Also known as: Apaf-1L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDAKARNCLL QHREALEKDI KTSYIMDHMI SNGVLSVIEE EKVKSQATQY
60 70 80 90 100
QRAAALIKMI LNKDNCAYIS FYNALLHEGY KDLAALLQSG LPLVSSSSGK
110 120 130 140 150
DTDGGITSFV RTVLCEGGVP QRPVIFVTRK KLVHAIQQKL WKLNGEPGWV
160 170 180 190 200
TIYGMAGCGK SVLAAEAVRD HSLLEGCFSG GVHWVSIGKQ DKSGLLMKLQ
210 220 230 240 250
NLCMRLDQEE SFSQRLPLNI EEAKDRLRVL MLRKHPRSLL ILDDVWDPWV
260 270 280 290 300
LKAFDNQCQI LLTTRDKSVT DSVMGPKHVV PVESGLGREK GLEILSLFVN
310 320 330 340 350
MKKEDLPAEA HSIIKECKGS PLVVSLIGAL LRDFPNRWAY YLRQLQNKQF
360 370 380 390 400
KRIRKSSSYD YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL
410 420 430 440 450
CVLWDLETEE VEDILQEFVN KSLLFCNRNG KSFCYYLHDL QVDFLTEKNR
460 470 480 490 500
SQLQDLHRKM VTQFQRYYQP HTLSPDQEDC MYWYNFLAYH MASANMHKEL
510 520 530 540 550
CALMFSLDWI KAKTELVGPA HLIHEFVAYR HILDEKDCAV CENFQEFLSL
560 570 580 590 600
NGHLLGRQPF PNIVQLGLCE PETSEVYRQA KLQAKQEGDT GRLYLEWINK
610 620 630 640 650
KTIKNLSRLV VRPHTDAVYH ACFSQDGQRI ASCGADKTLQ VFKAETGEKL
660 670 680 690 700
LDIKAHEDEV LCCAFSSDDS YIATCSADKK VKIWDSATGK LVHTYDEHSE
710 720 730 740 750
QVNCCHFTNK SNHLLLATGS NDFFLKLWDL NQKECRNTMF GHTNSVNHCR
760 770 780 790 800
FSPDDELLAS CSADGTLRLW DVRSANERKS INVKRFFLSS EDPPEDVEVI
810 820 830 840 850
VKCCSWSADG DKIIVAAKNK VLLFDIHTSG LLAEIHTGHH STIQYCDFSP
860 870 880 890 900
YDHLAVIALS QYCVELWNID SRLKVADCRG HLSWVHGVMF SPDGSSFLTA
910 920 930 940 950
SDDQTIRVWE TKKVCKNSAI VLKQEIDVVF QENETMVLAV DNIRGLQLIA
960 970 980 990 1000
GKTGQIDYLP EAQVSCCCLS PHLEYVAFGD EDGAIKIIEL PNNRVFSSGV
1010 1020 1030 1040 1050
GHKKAVRHIQ FTADGKTLIS SSEDSVIQVW NWQTGDYVFL QAHQETVKDF
1060 1070 1080 1090 1100
RLLQDSRLLS WSFDGTVKVW NVITGRIERD FTCHQGTVLS CAISSDATKF
1110 1120 1130 1140 1150
SSTSADKTAK IWSFDLLSPL HELKGHNGCV RCSAFSLDGI LLATGDDNGE
1160 1170 1180 1190 1200
IRIWNVSDGQ LLHSCAPISV EEGTATHGGW VTDVCFSPDS KTLVSAGGYL
1210 1220 1230 1240
KWWNVATGDS SQTFYTNGTN LKKIHVSPDF RTYVTVDNLG ILYILQVLE
Note: Major isoform.
Length:1,249
Mass (Da):141,003
Last modified:July 27, 2011 - v3
Checksum:iAA639E407F9ABC24
GO
Isoform 2 (identifier: O88879-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     99-110: GKDTDGGITSFV → A

Show »
Length:1,238
Mass (Da):139,896
Checksum:iCC9BE41A95B20ADF
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3XA09G3XA09_MOUSE
Apoptotic protease-activating facto...
Apaf1 mCG_10338
1,238Annotation score:
Q80VR5Q80VR5_MOUSE
Apaf1 protein
Apaf1
258Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209E → D in AAC62458 (PubMed:9753320).Curated1
Sequence conflicti592R → P in AAC62458 (PubMed:9753320).Curated1
Sequence conflicti710K → S in AAC62458 (PubMed:9753320).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00676399 – 110GKDTD…ITSFV → A in isoform 2. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064071 mRNA Translation: AAC62458.1
BC131683 mRNA Translation: AAI31684.1
BC131684 mRNA Translation: AAI31685.1
CCDSiCCDS36030.1 [O88879-1]
CCDS70095.1 [O88879-2]
RefSeqiNP_001036023.1, NM_001042558.1 [O88879-1]
NP_033814.2, NM_009684.2 [O88879-1]
UniGeneiMm.220289

Genome annotation databases

EnsembliENSMUST00000020157; ENSMUSP00000020157; ENSMUSG00000019979 [O88879-1]
ENSMUST00000159110; ENSMUSP00000125291; ENSMUSG00000019979 [O88879-1]
GeneIDi11783
KEGGimmu:11783
UCSCiuc007gtg.1 mouse [O88879-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064071 mRNA Translation: AAC62458.1
BC131683 mRNA Translation: AAI31684.1
BC131684 mRNA Translation: AAI31685.1
CCDSiCCDS36030.1 [O88879-1]
CCDS70095.1 [O88879-2]
RefSeqiNP_001036023.1, NM_001042558.1 [O88879-1]
NP_033814.2, NM_009684.2 [O88879-1]
UniGeneiMm.220289

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SFZX-ray3.00A1-1249[»]
3SHFX-ray3.55A1-1249[»]
ProteinModelPortaliO88879
SMRiO88879
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198139, 1 interactor
CORUMiO88879
IntActiO88879, 1 interactor
STRINGi10090.ENSMUSP00000020157

PTM databases

iPTMnetiO88879
PhosphoSitePlusiO88879

Proteomic databases

EPDiO88879
MaxQBiO88879
PaxDbiO88879
PeptideAtlasiO88879
PRIDEiO88879

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020157; ENSMUSP00000020157; ENSMUSG00000019979 [O88879-1]
ENSMUST00000159110; ENSMUSP00000125291; ENSMUSG00000019979 [O88879-1]
GeneIDi11783
KEGGimmu:11783
UCSCiuc007gtg.1 mouse [O88879-1]

Organism-specific databases

CTDi317
MGIiMGI:1306796 Apaf1

Phylogenomic databases

eggNOGiKOG4155 Eukaryota
KOG4658 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOVERGENiHBG018730
InParanoidiO88879
KOiK02084
OMAiSRIGHKK
OrthoDBiEOG091G036U
PhylomeDBiO88879
TreeFamiTF323866

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiApaf1 mouse
PROiPR:O88879
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019979 Expressed in 266 organ(s), highest expression level in epithelium of small intestine
CleanExiMM_APAF1
ExpressionAtlasiO88879 baseline and differential
GenevisibleiO88879 MM

Family and domain databases

CDDicd08323 CARD_APAF1, 1 hit
Gene3Di1.10.10.10, 1 hit
2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR017251 Apaf-1
IPR037963 APAF1_CARD_dom
IPR024977 Apc4_WD40_dom
IPR001315 CARD
IPR011029 DEATH-like_dom_sf
IPR020472 G-protein_beta_WD-40_rep
IPR002182 NB-ARC
IPR027417 P-loop_NTPase
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 1 hit
PF00619 CARD, 1 hit
PF00931 NB-ARC, 1 hit
PF00400 WD40, 8 hits
PIRSFiPIRSF037646 Apop_pept_activating-1, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 13 hits
SUPFAMiSSF47986 SSF47986, 1 hit
SSF50978 SSF50978, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50209 CARD, 1 hit
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 9 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAPAF_MOUSE
AccessioniPrimary (citable) accession number: O88879
Secondary accession number(s): A2RRK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again