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Protein

ADP-ribosylation factor-like protein 6

Gene

Arl6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in membrane protein trafficking at the base of the ciliary organelle (By similarity). Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia (By similarity). Together with BBS1, is necessary for correct trafficking of PKD1 to primary cilia (PubMed:24939912). Together with the BBSome complex and LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation (By similarity). May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade (By similarity). Isoform 2 may be required for proper retinal function and organization (PubMed:20333246).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50MagnesiumBy similarity1
Binding sitei50GTPBy similarity1
Binding sitei72GTP; via amide nitrogenBy similarity1
Binding sitei164GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi69 – 73GTPBy similarity5
Nucleotide bindingi130 – 133GTPBy similarity4

GO - Molecular functioni

  • GTP binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • phospholipid binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processCilium biogenesis/degradation, Protein transport, Transport
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5620922 BBSome-mediated cargo-targeting to cilium

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 6
Gene namesi
Name:Arl6
Synonyms:Bbs3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1927136 Arl6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Isoform 2 deficient mice present a disruption of the normal photoreceptor architecture.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074732 – 186ADP-ribosylation factor-like protein 6Add BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

EPDiO88848
PaxDbiO88848
PeptideAtlasiO88848
PRIDEiO88848

PTM databases

PhosphoSitePlusiO88848

Expressioni

Tissue specificityi

Most abundant in brain and kidney. Expressed in heart and eye. Isoform 2 is expressed only in the retina.1 Publication

Gene expression databases

BgeeiENSMUSG00000022722 Expressed in 281 organ(s), highest expression level in camera-type eye
CleanExiMM_ARL6
ExpressionAtlasiO88848 baseline and differential
GenevisibleiO88848 MM

Interactioni

Subunit structurei

Interacts with SEC61B, ARL6IP1, ARL6IP2, ARL6IP3, ARL6IP4 ARL6IP5 and ARL6IP6. Interacts (GTP-bound form) with the BBSome a complex that contains BBS1, BBS2, BBS4, BBS5, BBS7, BBS8/TTC8, BBS9 and BBIP10. Interacts (GTP-free form) with IFT27.By similarity

Protein-protein interaction databases

CORUMiO88848
IntActiO88848, 10 interactors
STRINGi10090.ENSMUSP00000023405

Structurei

3D structure databases

ProteinModelPortaliO88848
SMRiO88848
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00920000149001
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiO88848
KOiK07951
OMAiIMVPTVG
PhylomeDBiO88848
TreeFamiTF105466

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O88848-1) [UniParc]FASTAAdd to basket
Also known as: Bbs3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGLLDRLSGL LGLKKKEVHV LCLGLDNSGK TTIINKLKPS NAQSQDIVPT
60 70 80 90 100
IGFSIEKFKS SSLSFTVFDM SGQGRYRNLW EHYYKDGQAI IFVIDSSDKL
110 120 130 140 150
RMVVAKEELD TLLNHPDIKH RRIPILFFAN KMDLRDSVTS VKVSQLLCLE
160 170 180
SIKDKPWHIC ASDAIKGEGL QEGVDWLQDQ IQAVKT
Length:186
Mass (Da):20,959
Last modified:November 1, 1998 - v1
Checksum:i4F26947318977386
GO
Isoform 2 (identifier: O88848-2) [UniParc]FASTAAdd to basket
Also known as: Bbs3l

The sequence of this isoform differs from the canonical sequence as follows:
     179-186: DQIQAVKT → EKTVQSDPSCEDVKR

Show »
Length:193
Mass (Da):21,778
Checksum:iFF91E45A2D785B7C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z067D3Z067_MOUSE
ADP-ribosylation factor-like protei...
Arl6
194Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040512179 – 186DQIQAVKT → EKTVQSDPSCEDVKR in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031903 mRNA Translation: AAC62194.1
AK158832 mRNA Translation: BAE34686.1
AK003778 mRNA Translation: BAB22990.1
CCDSiCCDS28259.1 [O88848-1]
CCDS84248.1 [O88848-2]
RefSeqiNP_001334173.1, NM_001347244.1 [O88848-2]
NP_062639.3, NM_019665.3 [O88848-1]
UniGeneiMm.392505

Genome annotation databases

EnsembliENSMUST00000023405; ENSMUSP00000023405; ENSMUSG00000022722 [O88848-1]
ENSMUST00000099646; ENSMUSP00000097238; ENSMUSG00000022722 [O88848-2]
ENSMUST00000118438; ENSMUSP00000113127; ENSMUSG00000022722 [O88848-2]
GeneIDi56297
KEGGimmu:56297
UCSCiuc007zpl.2 mouse [O88848-1]
uc007zpm.2 mouse [O88848-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031903 mRNA Translation: AAC62194.1
AK158832 mRNA Translation: BAE34686.1
AK003778 mRNA Translation: BAB22990.1
CCDSiCCDS28259.1 [O88848-1]
CCDS84248.1 [O88848-2]
RefSeqiNP_001334173.1, NM_001347244.1 [O88848-2]
NP_062639.3, NM_019665.3 [O88848-1]
UniGeneiMm.392505

3D structure databases

ProteinModelPortaliO88848
SMRiO88848
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiO88848
IntActiO88848, 10 interactors
STRINGi10090.ENSMUSP00000023405

PTM databases

PhosphoSitePlusiO88848

Proteomic databases

EPDiO88848
PaxDbiO88848
PeptideAtlasiO88848
PRIDEiO88848

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023405; ENSMUSP00000023405; ENSMUSG00000022722 [O88848-1]
ENSMUST00000099646; ENSMUSP00000097238; ENSMUSG00000022722 [O88848-2]
ENSMUST00000118438; ENSMUSP00000113127; ENSMUSG00000022722 [O88848-2]
GeneIDi56297
KEGGimmu:56297
UCSCiuc007zpl.2 mouse [O88848-1]
uc007zpm.2 mouse [O88848-2]

Organism-specific databases

CTDi84100
MGIiMGI:1927136 Arl6

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00920000149001
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiO88848
KOiK07951
OMAiIMVPTVG
PhylomeDBiO88848
TreeFamiTF105466

Enzyme and pathway databases

ReactomeiR-MMU-5620922 BBSome-mediated cargo-targeting to cilium

Miscellaneous databases

PROiPR:O88848
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022722 Expressed in 281 organ(s), highest expression level in camera-type eye
CleanExiMM_ARL6
ExpressionAtlasiO88848 baseline and differential
GenevisibleiO88848 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARL6_MOUSE
AccessioniPrimary (citable) accession number: O88848
Secondary accession number(s): Q3TY77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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