Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate dehydrogenase [NADP] cytoplasmic

Gene

Idh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.1 Publication

Activity regulationi

Irreversibly inhibited by Cd2+ concentrations above 50 µM.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateCombined sources1 Publication1
Binding sitei82NADPCombined sources1 Publication1
Binding sitei109SubstrateCombined sources1 Publication1
Binding sitei132SubstrateCombined sources1 Publication1
Sitei139Critical for catalysisBy similarity1
Binding sitei212Substrate; shared with neighboring subunitCombined sources1 Publication1
Sitei212Critical for catalysisBy similarity1
Metal bindingi252Magnesium or manganese; shared with neighboring subunit1 Publication1
Binding sitei260NADPCombined sources1 Publication1
Metal bindingi275Magnesium or manganese1 Publication1
Metal bindingi279Magnesium or manganese1 Publication1
Binding sitei328NADP; via amide nitrogen and carbonyl oxygenCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPCombined sources1 Publication3
Nucleotide bindingi310 – 315NADPCombined sources1 Publication6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.1.1.42 3474
ReactomeiR-MMU-389542 NADPH regeneration
R-MMU-6798695 Neutrophil degranulation
R-MMU-9033241 Peroxisomal protein import

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.421 Publication)
Short name:
IDH
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:Idh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:96413 Idh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi245C → S: No effect on inhibition by cadmium ions. 1 Publication1
Mutagenesisi379C → S: Decreased inhibition by cadmium ions. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000835782 – 414Isocitrate dehydrogenase [NADP] cytoplasmicAdd BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei42PhosphotyrosineBy similarity1
Modified residuei81N6-acetyllysineCombined sources1
Modified residuei126N6-succinyllysineCombined sources1
Modified residuei224N6-acetyllysineCombined sources1
Modified residuei233N6-acetyllysineCombined sources1
Modified residuei243N6-acetyllysineCombined sources1
Modified residuei321N6-acetyllysineBy similarity1
Modified residuei389PhosphoserineCombined sources1
Modified residuei400N6-succinyllysineCombined sources1

Post-translational modificationi

Acetylation at Lys-374 dramatically reduces catalytic activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO88844
MaxQBiO88844
PaxDbiO88844
PeptideAtlasiO88844
PRIDEiO88844

2D gel databases

COMPLUYEAST-2DPAGEiO88844
REPRODUCTION-2DPAGEiO88844
SWISS-2DPAGEiO88844

PTM databases

CarbonylDBiO88844
iPTMnetiO88844
PhosphoSitePlusiO88844
SwissPalmiO88844

Expressioni

Gene expression databases

BgeeiENSMUSG00000025950 Expressed in 344 organ(s), highest expression level in cumulus cell
CleanExiMM_IDH1
ExpressionAtlasiO88844 baseline and differential
GenevisibleiO88844 MM

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200510, 2 interactors
IntActiO88844, 6 interactors
MINTiO88844
STRINGi10090.ENSMUSP00000095316

Structurei

Secondary structure

1414
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO88844
SMRiO88844
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88844

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingCombined sources1 Publication7

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1526 Eukaryota
COG0538 LUCA
GeneTreeiENSGT00390000012547
HOGENOMiHOG000019858
HOVERGENiHBG006119
InParanoidiO88844
KOiK00031
OMAiHGTVQRH
OrthoDBiEOG091G06IY
TreeFamiTF300428

Family and domain databases

InterProiView protein in InterPro
IPR019818 IsoCit/isopropylmalate_DH_CS
IPR004790 Isocitrate_DH_NADP
IPR024084 IsoPropMal-DH-like_dom
PANTHERiPTHR11822 PTHR11822, 1 hit
PfamiView protein in Pfam
PF00180 Iso_dh, 1 hit
PIRSFiPIRSF000108 IDH_NADP, 1 hit
SMARTiView protein in SMART
SM01329 Iso_dh, 1 hit
TIGRFAMsiTIGR00127 nadp_idh_euk, 1 hit
PROSITEiView protein in PROSITE
PS00470 IDH_IMDH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

O88844-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRKIQGGSV VEMQGDEMTR IIWELIKEKL ILPYVELDLH SYDLGIENRD
60 70 80 90 100
ATNDQVTKDA AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNIPRL VTGWVKPIII GRHAYGDQYR ATDFVVPGPG
160 170 180 190 200
KVEITYTPKD GTQKVTYMVH DFEEGGGVAM GMYNQDKSIE DFAHSSFQMA
210 220 230 240 250
LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKKYKSQFE AQKICYEHRL
260 270 280 290 300
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG
310 320 330 340 350
KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWSRGL AHRAKLDNNT
360 370 380 390 400
ELSFFAKALE DVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
410
LGENLKAKLA QAKL
Length:414
Mass (Da):46,674
Last modified:July 27, 2011 - v2
Checksum:iC7FEF315D042C44C
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YVY3D3YVY3_MOUSE
Isocitrate dehydrogenase [NADP] cyt...
Idh1
143Annotation score:
A0A087WRS9A0A087WRS9_MOUSE
Isocitrate dehydrogenase [NADP] cyt...
Idh1
202Annotation score:
A0A087WPT4A0A087WPT4_MOUSE
Isocitrate dehydrogenase [NADP] cyt...
Idh1
187Annotation score:
A0A087WRM4A0A087WRM4_MOUSE
Isocitrate dehydrogenase [NADP] cyt...
Idh1
84Annotation score:
A0A087WNN7A0A087WNN7_MOUSE
Isocitrate dehydrogenase [NADP] cyt...
Idh1
19Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243K → N in AAD02919 (PubMed:9866202).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020039 mRNA Translation: AAD02919.1
AK149019 mRNA Translation: BAE28720.1
AK151212 mRNA Translation: BAE30207.1
AK159173 mRNA Translation: BAE34873.1
AK160896 mRNA Translation: BAE36075.1
AK167158 mRNA Translation: BAE39299.1
CH466548 Genomic DNA Translation: EDL00223.1
CH466548 Genomic DNA Translation: EDL00225.1
CCDSiCCDS15016.1
RefSeqiNP_001104790.1, NM_001111320.1
NP_034627.3, NM_010497.3
UniGeneiMm.9925

Genome annotation databases

EnsembliENSMUST00000097709; ENSMUSP00000095316; ENSMUSG00000025950
ENSMUST00000169032; ENSMUSP00000127307; ENSMUSG00000025950
GeneIDi15926
KEGGimmu:15926
UCSCiuc007bhn.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020039 mRNA Translation: AAD02919.1
AK149019 mRNA Translation: BAE28720.1
AK151212 mRNA Translation: BAE30207.1
AK159173 mRNA Translation: BAE34873.1
AK160896 mRNA Translation: BAE36075.1
AK167158 mRNA Translation: BAE39299.1
CH466548 Genomic DNA Translation: EDL00223.1
CH466548 Genomic DNA Translation: EDL00225.1
CCDSiCCDS15016.1
RefSeqiNP_001104790.1, NM_001111320.1
NP_034627.3, NM_010497.3
UniGeneiMm.9925

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5YZHX-ray1.99A/B1-414[»]
5YZIX-ray2.52A/B1-414[»]
ProteinModelPortaliO88844
SMRiO88844
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200510, 2 interactors
IntActiO88844, 6 interactors
MINTiO88844
STRINGi10090.ENSMUSP00000095316

PTM databases

CarbonylDBiO88844
iPTMnetiO88844
PhosphoSitePlusiO88844
SwissPalmiO88844

2D gel databases

COMPLUYEAST-2DPAGEiO88844
REPRODUCTION-2DPAGEiO88844
SWISS-2DPAGEiO88844

Proteomic databases

EPDiO88844
MaxQBiO88844
PaxDbiO88844
PeptideAtlasiO88844
PRIDEiO88844

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097709; ENSMUSP00000095316; ENSMUSG00000025950
ENSMUST00000169032; ENSMUSP00000127307; ENSMUSG00000025950
GeneIDi15926
KEGGimmu:15926
UCSCiuc007bhn.2 mouse

Organism-specific databases

CTDi3417
MGIiMGI:96413 Idh1

Phylogenomic databases

eggNOGiKOG1526 Eukaryota
COG0538 LUCA
GeneTreeiENSGT00390000012547
HOGENOMiHOG000019858
HOVERGENiHBG006119
InParanoidiO88844
KOiK00031
OMAiHGTVQRH
OrthoDBiEOG091G06IY
TreeFamiTF300428

Enzyme and pathway databases

BRENDAi1.1.1.42 3474
ReactomeiR-MMU-389542 NADPH regeneration
R-MMU-6798695 Neutrophil degranulation
R-MMU-9033241 Peroxisomal protein import

Miscellaneous databases

EvolutionaryTraceiO88844
PROiPR:O88844
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025950 Expressed in 344 organ(s), highest expression level in cumulus cell
CleanExiMM_IDH1
ExpressionAtlasiO88844 baseline and differential
GenevisibleiO88844 MM

Family and domain databases

InterProiView protein in InterPro
IPR019818 IsoCit/isopropylmalate_DH_CS
IPR004790 Isocitrate_DH_NADP
IPR024084 IsoPropMal-DH-like_dom
PANTHERiPTHR11822 PTHR11822, 1 hit
PfamiView protein in Pfam
PF00180 Iso_dh, 1 hit
PIRSFiPIRSF000108 IDH_NADP, 1 hit
SMARTiView protein in SMART
SM01329 Iso_dh, 1 hit
TIGRFAMsiTIGR00127 nadp_idh_euk, 1 hit
PROSITEiView protein in PROSITE
PS00470 IDH_IMDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIDHC_MOUSE
AccessioniPrimary (citable) accession number: O88844
Secondary accession number(s): Q3UAV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again