Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SPRY domain-containing SOCS box protein 2

Gene

Spsb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
SPRY domain-containing SOCS box protein 2
Short name:
SSB-2
Alternative name(s):
Gene-rich cluster protein C9
Gene namesi
Name:Spsb2
Synonyms:Grcc9, Ssb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1315199 Spsb2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi120Y → A: Loss of interaction with PARW. No effect on interaction with MET. 2 Publications1
Mutagenesisi123 – 125LLG → AAA: Loss of interaction with MET and PARW. 2 Publications3
Mutagenesisi126 – 128SNS → AAA: Loss of interaction with PAWR. 1 Publication3
Mutagenesisi141 – 143YHQ → AAA: Strongly reduced interaction with MET and PARW. 1 Publication3
Mutagenesisi151 – 152QY → AA: Loss of interaction with MET and PARW. 1 Publication2
Mutagenesisi188 – 189GP → AA: Strongly reduced interaction with MET. Loss of interaction with PARW. 1 Publication2
Mutagenesisi198T → A: No loss of interaction with PAWR. 1 Publication1
Mutagenesisi206V → A: Loss of interaction with PARW. No effect on interaction with MET. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL3325309

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002384751 – 264SPRY domain-containing SOCS box protein 2Add BLAST264

Proteomic databases

MaxQBiO88838
PaxDbiO88838
PRIDEiO88838

PTM databases

PhosphoSitePlusiO88838

Expressioni

Gene expression databases

BgeeiENSMUSG00000038451 Expressed in 188 organ(s), highest expression level in submandibular gland
ExpressionAtlasiO88838 baseline and differential
GenevisibleiO88838 MM

Interactioni

Subunit structurei

Component of the probable ECS(SPSB2) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB2 (By similarity). Interacts with CUL5, RNF7, ELOB and ELOC (By similarity). Interacts with MET (PubMed:16369487). Interacts (via B30.2/SPRY domain) with PAWR; this interaction occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:20561531).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PAWRQ96IZ06EBI-8820410,EBI-595869From Homo sapiens.

Protein-protein interaction databases

BioGridi200055, 1 interactor
DIPiDIP-29001N
IntActiO88838, 2 interactors
STRINGi10090.ENSMUSP00000041585

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO88838
SMRiO88838
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88838

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 221B30.2/SPRYPROSITE-ProRule annotationAdd BLAST196
Domaini222 – 264SOCS boxPROSITE-ProRule annotationAdd BLAST43

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.By similarity

Sequence similaritiesi

Belongs to the SPSB family.Curated

Phylogenomic databases

eggNOGiKOG3953 Eukaryota
ENOG410XQC1 LUCA
GeneTreeiENSGT00390000009402
HOGENOMiHOG000230524
HOVERGENiHBG055793
InParanoidiO88838
KOiK10344
OMAiCQVRISY
OrthoDBiEOG091G0DF0
PhylomeDBiO88838
TreeFamiTF312822

Family and domain databases

CDDicd03719 SOCS_SSB2, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR001496 SOCS_box
IPR036036 SOCS_box-like_dom_sf
IPR003877 SPRY_dom
IPR037340 SSB2_SOCS
PfamiView protein in Pfam
PF07525 SOCS_box, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00253 SOCS, 1 hit
SM00969 SOCS_box, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF158235 SSF158235, 1 hit
SSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50225 SOCS, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O88838-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGQTALARGS SSTPTSQALY SDFSPPEGLE ELLSAPPPDL VAQRHHGWNP
60 70 80 90 100
KDCSENIDVK EGGLCFERRP VAQSTDGVRG KRGYSRGLHA WEISWPLEQR
110 120 130 140 150
GTHAVVGVAT ALAPLQADHY AALLGSNSES WGWDIGRGKL YHQSKGLEAP
160 170 180 190 200
QYPAGPQGEQ LVVPERLLVV LDMEEGTLGY SIGGTYLGPA FRGLKGRTLY
210 220 230 240 250
PSVSAVWGQC QVRIRYMGER RVEEPQSLLH LSRLCVRHAL GDTRLGQIST
260
LPLPPAMKRY LLYK
Length:264
Mass (Da):28,938
Last modified:November 1, 1998 - v1
Checksum:iE71985597D9B38CB
GO
Isoform 2 (identifier: O88838-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-264: VEEPQSLLHL...PAMKRYLLYK → GEIRTRCGEI...VGFLSLWPVT

Note: No experimental confirmation available.
Show »
Length:258
Mass (Da):28,014
Checksum:iE23A2DDAFDFB6773
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YX10D3YX10_MOUSE
SPRY domain-containing SOCS box pro...
Spsb2
244Annotation score:
D3Z7N7D3Z7N7_MOUSE
SPRY domain-containing SOCS box pro...
Spsb2
211Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018613222 – 264VEEPQ…YLLYK → GEIRTRCGEITTLGNGLGWK LMVGAQEVGFLSLWPVT in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA Translation: AAC36017.1
AF403037 mRNA Translation: AAL57356.1
AK036451 mRNA Translation: BAC29435.1
AK148177 mRNA Translation: BAE28397.1
BC002005 mRNA Translation: AAH02005.1
BC010305 mRNA Translation: AAH10305.1
CCDSiCCDS20529.1 [O88838-1]
RefSeqiNP_001292979.1, NM_001306050.1 [O88838-1]
NP_001292980.1, NM_001306051.1 [O88838-1]
NP_001292981.1, NM_001306052.1 [O88838-1]
NP_001292982.1, NM_001306053.1
NP_038567.1, NM_013539.2 [O88838-1]
XP_006505630.1, XM_006505567.3 [O88838-1]
UniGeneiMm.261906

Genome annotation databases

EnsembliENSMUST00000047760; ENSMUSP00000041585; ENSMUSG00000038451 [O88838-1]
ENSMUST00000052727; ENSMUSP00000060124; ENSMUSG00000038451 [O88838-1]
ENSMUST00000112473; ENSMUSP00000108092; ENSMUSG00000038451 [O88838-2]
GeneIDi14794
KEGGimmu:14794
UCSCiuc009drw.1 mouse [O88838-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA Translation: AAC36017.1
AF403037 mRNA Translation: AAL57356.1
AK036451 mRNA Translation: BAC29435.1
AK148177 mRNA Translation: BAE28397.1
BC002005 mRNA Translation: AAH02005.1
BC010305 mRNA Translation: AAH10305.1
CCDSiCCDS20529.1 [O88838-1]
RefSeqiNP_001292979.1, NM_001306050.1 [O88838-1]
NP_001292980.1, NM_001306051.1 [O88838-1]
NP_001292981.1, NM_001306052.1 [O88838-1]
NP_001292982.1, NM_001306053.1
NP_038567.1, NM_013539.2 [O88838-1]
XP_006505630.1, XM_006505567.3 [O88838-1]
UniGeneiMm.261906

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AFJNMR-A12-224[»]
3EK9X-ray2.60A12-224[»]
ProteinModelPortaliO88838
SMRiO88838
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200055, 1 interactor
DIPiDIP-29001N
IntActiO88838, 2 interactors
STRINGi10090.ENSMUSP00000041585

Chemistry databases

ChEMBLiCHEMBL3325309

PTM databases

PhosphoSitePlusiO88838

Proteomic databases

MaxQBiO88838
PaxDbiO88838
PRIDEiO88838

Protocols and materials databases

DNASUi14794
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047760; ENSMUSP00000041585; ENSMUSG00000038451 [O88838-1]
ENSMUST00000052727; ENSMUSP00000060124; ENSMUSG00000038451 [O88838-1]
ENSMUST00000112473; ENSMUSP00000108092; ENSMUSG00000038451 [O88838-2]
GeneIDi14794
KEGGimmu:14794
UCSCiuc009drw.1 mouse [O88838-1]

Organism-specific databases

CTDi84727
MGIiMGI:1315199 Spsb2

Phylogenomic databases

eggNOGiKOG3953 Eukaryota
ENOG410XQC1 LUCA
GeneTreeiENSGT00390000009402
HOGENOMiHOG000230524
HOVERGENiHBG055793
InParanoidiO88838
KOiK10344
OMAiCQVRISY
OrthoDBiEOG091G0DF0
PhylomeDBiO88838
TreeFamiTF312822

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

EvolutionaryTraceiO88838
PROiPR:O88838
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038451 Expressed in 188 organ(s), highest expression level in submandibular gland
ExpressionAtlasiO88838 baseline and differential
GenevisibleiO88838 MM

Family and domain databases

CDDicd03719 SOCS_SSB2, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR001496 SOCS_box
IPR036036 SOCS_box-like_dom_sf
IPR003877 SPRY_dom
IPR037340 SSB2_SOCS
PfamiView protein in Pfam
PF07525 SOCS_box, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00253 SOCS, 1 hit
SM00969 SOCS_box, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF158235 SSF158235, 1 hit
SSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50225 SOCS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPSB2_MOUSE
AccessioniPrimary (citable) accession number: O88838
Secondary accession number(s): Q8CBA5, Q91Z15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again