UniProtKB - O88833 (CP4AA_MOUSE)
Cytochrome P450 4A10
Cyp4a10
Functioni
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of long-chain fatty acids (By similarity).
Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver (PubMed:17112342) (By similarity).
In kidney, may play an important role in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (By similarity).
Also participates in the formation of anti-inflammatory hydroxyepoxyeicosatrienoic acids (HEETs) in kidney by converting 8,9-epoxyeicosatrienoic acid (EET) to 20,8,9-HEET, an activator of PPARA. Displays substantially lower fatty acid omega-1 hydroxylase activity (By similarity).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
By similarity1 PublicationCatalytic activityi
- an ω-methyl-long-chain fatty acid + O2 + reduced [NADPH—hemoprotein reductase] = an ω-hydroxy-long-chain fatty acid + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.80By similarityThis reaction proceeds in the forwardBy similarity direction.
- dodecanoate + O2 + reduced [NADPH—hemoprotein reductase] = 12-hydroxydodecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- dodecanoate + O2 + reduced [NADPH—hemoprotein reductase] = 11-hydroxydodecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- O2 + reduced [NADPH—hemoprotein reductase] + tetradecanoate = 14-hydroxytetradecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- hexadecanoate + O2 + reduced [NADPH—hemoprotein reductase] = 16-hydroxyhexadecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.80By similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoate + O2 + reduced [NADPH—hemoprotein reductase] = 18-hydroxy-(9Z)-octadecenoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.80By similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z,12Z)-octadecadienoate + O2 + reduced [NADPH—hemoprotein reductase] = 18-hydroxy-(9Z,12Z)-octadecadienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z,12Z)-octadecadienoate + O2 + reduced [NADPH—hemoprotein reductase] = 17-hydroxy-(9Z,12Z)-octadecadienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH—hemoprotein reductase] = 20-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- 8,9-epoxy-(5Z,11Z,14Z)-eicosatrienoate + O2 + reduced [NADPH—hemoprotein reductase] = 20-hydroxy-8,9-epoxy-(5Z,11Z,14Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Kineticsi
- KM=2 µM for dodecanoate1 Publication
- Vmax=55 nmol/min/nmol enzyme toward dodecanoate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 320 | Heme; covalent, via 1 linkBy similarity | 1 | |
Metal bindingi | 456 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- 16-hydroxypalmitate dehydrogenase activity Source: MGI
- alkane 1-monooxygenase activity Source: MGI
- arachidonic acid epoxygenase activity Source: MGI
- arachidonic acid monooxygenase activity Source: MGI
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- laurate hydroxylase activity Source: MGI
- leukotriene-B4 20-monooxygenase activity Source: MGI
- long-chain fatty acid omega-hydroxylase activity Source: MGI
- monooxygenase activity Source: UniProtKB
GO - Biological processi
- arachidonic acid metabolic process Source: MGI
- fatty acid metabolic process Source: UniProtKB
- icosanoid biosynthetic process Source: MGI
- kidney development Source: GO_Central
- lauric acid metabolic process Source: MGI
- linoleic acid metabolic process Source: MGI
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
Reactomei | R-MMU-211935, Fatty acids R-MMU-211958, Miscellaneous substrates R-MMU-211979, Eicosanoids R-MMU-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) |
Chemistry databases
SwissLipidsi | SLP:000000399 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 4A10Alternative name(s): CYPIVA10 Cytochrome P450-LA-omega 1 Cytochrome P452 Lauric acid omega-hydroxylase Long-chain fatty acid omega-monooxygenase (EC:1.14.14.80By similarity) |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88611, Cyp4a10 |
VEuPathDBi | HostDB:ENSMUSG00000066072 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
- Microsome membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular space Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 15 – 35 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 121 – 141 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051815 | 1 – 509 | Cytochrome P450 4A10Add BLAST | 509 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 439 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | O88833 |
MaxQBi | O88833 |
PaxDbi | O88833 |
PRIDEi | O88833 |
ProteomicsDBi | 284152 |
PTM databases
iPTMneti | O88833 |
PhosphoSitePlusi | O88833 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000066072, Expressed in cortex of kidney and 71 other tissues |
ExpressionAtlasi | O88833, baseline and differential |
Genevisiblei | O88833, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000061126 |
Miscellaneous databases
RNActi | O88833, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000163570 |
HOGENOMi | CLU_001570_5_1_1 |
InParanoidi | O88833 |
OMAi | YGVQVCM |
OrthoDBi | 1247045at2759 |
PhylomeDBi | O88833 |
TreeFami | TF105088 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSVSALSPTR FADSLSGFLQ VASVLGLLLL LVKAVQFYLH RQWLLKAFQQ
60 70 80 90 100
FPSPPFHWFF GHEKFKGDQE LQEIVSCIEN FPSAFPRWFW GSKAYLTVYD
110 120 130 140 150
PDYMKVILGR SDPKANGAYR LLAPWIGYGL LLLNGQPWFQ HRRMLTPAFH
160 170 180 190 200
YDILKPYVKN MADSIRLMLD KWERLADQDS SIEIFQHISL MTLDTVMKCA
210 220 230 240 250
FSHKGSVQVD GNYRTYLQAI GDLNNLFHSR VRNIFHQNDT IYKLSSNGRL
260 270 280 290 300
AKQACQLAHD HTDGVIKLRK DQLQDEGELE KIKKKRRLDF LDILLFARME
310 320 330 340 350
NGDSMSDKDL RAEVDTFMFE GHDTTASGVS WIFYALATHP DHQQRCREEV
360 370 380 390 400
QSLLGDGSSI TWDHLDQIPY TTMCIKEALR LYPPVPGIVR ELSTSVTFPD
410 420 430 440 450
GRSLPKGVQV TLSIYGLHHN PKVWPNPEVF DPSRFAPDSP RHSHSFLPFS
460 470 480 490 500
GGARNCIGKQ FAMSELKVIV ALTLLRFELL PDPTRVPMPL ARLVLKSKNG
IYLHLKKLH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2A977 | A2A977_MOUSE | Cytochrome P450 4A10 | Cyp4a10 | 499 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | H → L in X69296 (PubMed:8031839).Curated | 1 | |
Sequence conflicti | 64 | K → Q in BAA33804 (Ref. 2) Curated | 1 | |
Sequence conflicti | 64 | K → Q in AAH10747 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 64 | K → Q in AAH51049 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 69 | Q → H in BAA33804 (Ref. 2) Curated | 1 | |
Sequence conflicti | 69 | Q → H in AAH10747 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 69 | Q → H in AAH51049 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 234 | I → N in X69296 (PubMed:8031839).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69296 mRNA No translation available. AB018421 mRNA Translation: BAA33804.1 AK002528 mRNA Translation: BAB22165.1 AL627182 Genomic DNA No translation available. BC010747 mRNA Translation: AAH10747.1 BC051049 mRNA Translation: AAH51049.1 |
CCDSi | CCDS18492.1 |
PIRi | S47553 |
RefSeqi | NP_034141.3, NM_010011.3 |
Genome annotation databases
Ensembli | ENSMUST00000058785; ENSMUSP00000061126; ENSMUSG00000066072 |
GeneIDi | 13117 |
KEGGi | mmu:13117 |
UCSCi | uc008uex.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69296 mRNA No translation available. AB018421 mRNA Translation: BAA33804.1 AK002528 mRNA Translation: BAB22165.1 AL627182 Genomic DNA No translation available. BC010747 mRNA Translation: AAH10747.1 BC051049 mRNA Translation: AAH51049.1 |
CCDSi | CCDS18492.1 |
PIRi | S47553 |
RefSeqi | NP_034141.3, NM_010011.3 |
3D structure databases
AlphaFoldDBi | O88833 |
SMRi | O88833 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000061126 |
Chemistry databases
SwissLipidsi | SLP:000000399 |
PTM databases
iPTMneti | O88833 |
PhosphoSitePlusi | O88833 |
Proteomic databases
jPOSTi | O88833 |
MaxQBi | O88833 |
PaxDbi | O88833 |
PRIDEi | O88833 |
ProteomicsDBi | 284152 |
Protocols and materials databases
DNASUi | 13117 |
Genome annotation databases
Ensembli | ENSMUST00000058785; ENSMUSP00000061126; ENSMUSG00000066072 |
GeneIDi | 13117 |
KEGGi | mmu:13117 |
UCSCi | uc008uex.2, mouse |
Organism-specific databases
CTDi | 13117 |
MGIi | MGI:88611, Cyp4a10 |
VEuPathDBi | HostDB:ENSMUSG00000066072 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000163570 |
HOGENOMi | CLU_001570_5_1_1 |
InParanoidi | O88833 |
OMAi | YGVQVCM |
OrthoDBi | 1247045at2759 |
PhylomeDBi | O88833 |
TreeFami | TF105088 |
Enzyme and pathway databases
Reactomei | R-MMU-211935, Fatty acids R-MMU-211958, Miscellaneous substrates R-MMU-211979, Eicosanoids R-MMU-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) |
Miscellaneous databases
BioGRID-ORCSi | 13117, 5 hits in 43 CRISPR screens |
PROi | PR:O88833 |
RNActi | O88833, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000066072, Expressed in cortex of kidney and 71 other tissues |
ExpressionAtlasi | O88833, baseline and differential |
Genevisiblei | O88833, MM |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP4AA_MOUSE | |
Accessioni | O88833Primary (citable) accession number: O88833 Secondary accession number(s): A2A976, Q9DCS5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families