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Protein

Lathosterol oxidase

Gene

Sc5d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes a dehydrogenation to introduce C5-6 double bond into lathosterol.

Catalytic activityi

A Delta7-sterol + 2 ferrocytochrome b5 + O2 + 2 H+ = a Delta(5,7)-sterol + 2 ferricytochrome b5 + 2 H2O.By similarity

Cofactori

Fe cationBy similarity

GO - Molecular functioni

GO - Biological processi

  • cholesterol biosynthetic process via lathosterol Source: MGI
  • sterol biosynthetic process Source: GO_Central

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandIron

Enzyme and pathway databases

BRENDAi1.14.21.6 3474
ReactomeiR-MMU-6807047 Cholesterol biosynthesis via desmosterol
R-MMU-6807062 Cholesterol biosynthesis via lathosterol

Names & Taxonomyi

Protein namesi
Recommended name:
Lathosterol oxidase (EC:1.14.19.20)
Alternative name(s):
C-5 sterol desaturase
Delta(7)-sterol 5-desaturase
Delta(7)-sterol C5(6)-desaturase
Lathosterol 5-desaturase
Sterol-C5-desaturase
Gene namesi
Name:Sc5d
Synonyms:Sc5dl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1353611 Sc5d

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170291 – 299Lathosterol oxidaseAdd BLAST299

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO88822
PaxDbiO88822
PeptideAtlasiO88822
PRIDEiO88822

PTM databases

iPTMnetiO88822
PhosphoSitePlusiO88822

Expressioni

Gene expression databases

BgeeiENSMUSG00000032018 Expressed in 283 organ(s), highest expression level in liver
ExpressionAtlasiO88822 baseline and differential
GenevisibleiO88822 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057354

Structurei

3D structure databases

ProteinModelPortaliO88822
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi138 – 143Histidine box-16
Motifi151 – 155Histidine box-25
Motifi228 – 233Histidine box-36

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0872 Eukaryota
COG3000 LUCA
GeneTreeiENSGT00550000075101
HOGENOMiHOG000200579
HOVERGENiHBG012628
InParanoidiO88822
KOiK00227
OMAiHHTDHHM
OrthoDBiEOG091G0C85
TreeFamiTF300797

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O88822-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDLVLSAADY YFFTPYVYPA TWPEDNIIRQ TISLLIVTNL GAYILYFFCA
60 70 80 90 100
TLSYYFVYDH SLMKHPQFLK NQVSREIVFT VKSLPWISIP TVSLFLLELR
110 120 130 140 150
GYSKLYDDIG DFPNGWIHLM VSVVSFLFFT DMLIYWIHRG LHHRLVYKRI
160 170 180 190 200
HKPHHIWKIP TPFASHAFHP VDGFLQSLPY HIYPFVFPLH KVVYLGLYVL
210 220 230 240 250
VNVWTISIHD GDFRVPQILR PFINGSAHHT DHHMFFDYNY GQYFTLWDRI
260 270 280 290
GGSFKHPSSF EGKGPHSYVK NMTEKESNSF AENGCKGKKV GNGEFTKNK
Length:299
Mass (Da):35,062
Last modified:September 18, 2013 - v2
Checksum:i96F3AFF61F7ED707
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1L1SSH0A0A1L1SSH0_MOUSE
Lathosterol oxidase
Sc5d
215Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136W → R in BAA33730 (PubMed:10786622).Curated1
Sequence conflicti291G → S in BAA33730 (PubMed:10786622).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016248 mRNA Translation: BAA33730.1
AK043825 mRNA Translation: BAC31666.1
AK077670 mRNA Translation: BAC36944.1
AC160051 Genomic DNA No translation available.
CCDSiCCDS23086.1
RefSeqiNP_766357.1, NM_172769.2
XP_006510316.1, XM_006510253.2
UniGeneiMm.32700
Mm.392107
Mm.470142

Genome annotation databases

EnsembliENSMUST00000052725; ENSMUSP00000057354; ENSMUSG00000032018
ENSMUST00000169609; ENSMUSP00000130438; ENSMUSG00000032018
GeneIDi235293
KEGGimmu:235293
UCSCiuc009par.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016248 mRNA Translation: BAA33730.1
AK043825 mRNA Translation: BAC31666.1
AK077670 mRNA Translation: BAC36944.1
AC160051 Genomic DNA No translation available.
CCDSiCCDS23086.1
RefSeqiNP_766357.1, NM_172769.2
XP_006510316.1, XM_006510253.2
UniGeneiMm.32700
Mm.392107
Mm.470142

3D structure databases

ProteinModelPortaliO88822
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057354

PTM databases

iPTMnetiO88822
PhosphoSitePlusiO88822

Proteomic databases

EPDiO88822
PaxDbiO88822
PeptideAtlasiO88822
PRIDEiO88822

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052725; ENSMUSP00000057354; ENSMUSG00000032018
ENSMUST00000169609; ENSMUSP00000130438; ENSMUSG00000032018
GeneIDi235293
KEGGimmu:235293
UCSCiuc009par.1 mouse

Organism-specific databases

CTDi6309
MGIiMGI:1353611 Sc5d

Phylogenomic databases

eggNOGiKOG0872 Eukaryota
COG3000 LUCA
GeneTreeiENSGT00550000075101
HOGENOMiHOG000200579
HOVERGENiHBG012628
InParanoidiO88822
KOiK00227
OMAiHHTDHHM
OrthoDBiEOG091G0C85
TreeFamiTF300797

Enzyme and pathway databases

BRENDAi1.14.21.6 3474
ReactomeiR-MMU-6807047 Cholesterol biosynthesis via desmosterol
R-MMU-6807062 Cholesterol biosynthesis via lathosterol

Miscellaneous databases

PROiPR:O88822
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032018 Expressed in 283 organ(s), highest expression level in liver
ExpressionAtlasiO88822 baseline and differential
GenevisibleiO88822 MM

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSC5D_MOUSE
AccessioniPrimary (citable) accession number: O88822
Secondary accession number(s): Q8BGI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 18, 2013
Last modified: November 7, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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