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Protein

Serotonin N-acetyltransferase

Gene

Aanat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.By similarity

Miscellaneous

Pineal melatonin synthesis is severely compromised in most inbred strains of mice. In C57BL/6, a polymorphism activates a cryptic splice site causing the production of an alternative form containing a premature stop codon. The predicted resulting protein would lack the putative catalytic and acetyl-CoA binding domains and therefore would be inactive.

Catalytic activityi

Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine.By similarity

Pathwayi: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetylserotonin O-methyltransferase (Asmt), Serotonin N-acetyltransferase (Aanat)
  2. no protein annotated in this organism
This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei118Important for the catalytic mechanism; involved in substrate deprotonationBy similarity1
Sitei120Important for the catalytic mechanism; involved in substrate deprotonationBy similarity1
Binding sitei122Substrate; via amide nitrogenBy similarity1
Binding sitei157Substrate; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processBiological rhythms, Melatonin biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-209931 Serotonin and melatonin biosynthesis
UniPathwayi
UPA00837;UER00815

Names & Taxonomyi

Protein namesi
Recommended name:
Serotonin N-acetyltransferase (EC:2.3.1.87By similarity)
Short name:
Serotonin acetylase
Alternative name(s):
Aralkylamine N-acetyltransferase
Short name:
AA-NAT
Gene namesi
Name:Aanat
Synonyms:Snat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1328365 Aanat

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745831 – 205Serotonin N-acetyltransferaseAdd BLAST205

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29Phosphothreonine; by PKABy similarity1
Modified residuei203PhosphoserineBy similarity1

Post-translational modificationi

cAMP-dependent phosphorylation on both N-terminal Thr-29 and C-terminal Ser-203 regulates AANAT activity by promoting interaction with 14-3-3 proteins.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88816
PRIDEiO88816

PTM databases

iPTMnetiO88816
PhosphoSitePlusiO88816

Expressioni

Tissue specificityi

Highly expressed in pineal gland at night. Expression in the retina has not been confirmed. Extrapineal expression could be strain-specific.1 Publication

Inductioni

Exhibits night/day variations with a drastically increased expression at night in the pineal gland.3 Publications

Gene expression databases

BgeeiENSMUSG00000020804 Expressed in 48 organ(s), highest expression level in cardiac ventricle
CleanExiMM_AANAT
ExpressionAtlasiO88816 baseline and differential
GenevisibleiO88816 MM

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with several 14-3-3 proteins, including YWHAB, YWHAE, YWHAG and YWHAZ, preferentially when phosphorylated at Thr-29 (By similarity). Phosphorylation on Ser-203 also allows binding to YWHAZ, but with lower affinity (By similarity). The interaction with YWHAZ considerably increases affinity for arylalkylamines and acetyl-CoA and protects the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000122895

Structurei

3D structure databases

ProteinModelPortaliO88816
SMRiO88816
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 194N-acetyltransferasePROSITE-ProRule annotationAdd BLAST162

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 124Acetyl-CoA bindingBy similarity3
Regioni130 – 135Acetyl-CoA bindingBy similarity6
Regioni166 – 168Acetyl-CoA bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4144 Eukaryota
COG0454 LUCA
GeneTreeiENSGT00390000015579
HOVERGENiHBG016332
InParanoidiO88816
KOiK00669
OMAiSILMWRY
OrthoDBiEOG091G0KJP
PhylomeDBiO88816
TreeFamiTF331622

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O88816-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLNINSLKPE ALHLPLGTSE FLGCQRRHTL PASEFRCLTP EDATSAFEIE
60 70 80 90 100
REAFISVSGT CPLYLDEIRH FLTLCPELSL GWFEEGCLVA FIIGSLWDKE
110 120 130 140 150
RLTQESLTLH RPGGRTAHLH VLAVHRTFRQ QGKGSVLLWR YLHHLGSQPA
160 170 180 190 200
VRRAVLMCED ALVPFYEKFG FQAVGPCAIT VGSLTFTELQ CSLRCHAFLR

RNSGC
Length:205
Mass (Da):23,069
Last modified:November 1, 1998 - v1
Checksum:iD2ECD070998CB643
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9QUP1Q9QUP1_MOUSE
Arylalkylamine N-acetyltransferase
Aanat
105Annotation score:
F2Z3V1F2Z3V1_MOUSE
Serotonin N-acetyltransferase
Aanat
59Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013358 mRNA Translation: BAA31526.1
AF004108 mRNA Translation: AAD09408.1
U83462 Genomic DNA Translation: AAD08637.1
CCDSiCCDS25671.1
RefSeqiNP_033721.1, NM_009591.3
UniGeneiMm.418559
Mm.42233

Genome annotation databases

EnsembliENSMUST00000153476; ENSMUSP00000122895; ENSMUSG00000020804
GeneIDi11298
KEGGimmu:11298
UCSCiuc007mlo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013358 mRNA Translation: BAA31526.1
AF004108 mRNA Translation: AAD09408.1
U83462 Genomic DNA Translation: AAD08637.1
CCDSiCCDS25671.1
RefSeqiNP_033721.1, NM_009591.3
UniGeneiMm.418559
Mm.42233

3D structure databases

ProteinModelPortaliO88816
SMRiO88816
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000122895

PTM databases

iPTMnetiO88816
PhosphoSitePlusiO88816

Proteomic databases

PaxDbiO88816
PRIDEiO88816

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000153476; ENSMUSP00000122895; ENSMUSG00000020804
GeneIDi11298
KEGGimmu:11298
UCSCiuc007mlo.2 mouse

Organism-specific databases

CTDi15
MGIiMGI:1328365 Aanat

Phylogenomic databases

eggNOGiKOG4144 Eukaryota
COG0454 LUCA
GeneTreeiENSGT00390000015579
HOVERGENiHBG016332
InParanoidiO88816
KOiK00669
OMAiSILMWRY
OrthoDBiEOG091G0KJP
PhylomeDBiO88816
TreeFamiTF331622

Enzyme and pathway databases

UniPathwayi
UPA00837;UER00815

ReactomeiR-MMU-209931 Serotonin and melatonin biosynthesis

Miscellaneous databases

PROiPR:O88816
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020804 Expressed in 48 organ(s), highest expression level in cardiac ventricle
CleanExiMM_AANAT
ExpressionAtlasiO88816 baseline and differential
GenevisibleiO88816 MM

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSNAT_MOUSE
AccessioniPrimary (citable) accession number: O88816
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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