UniProtKB - O88788 (AG10B_RAT)
Protein
Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
Gene
Alg10b
Organism
Rattus norvegicus (Rat)
Status
Functioni
Putative alpha-1,2-glucosyltransferase, which adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc2-PP-Dol (By similarity). When coupled to KCNH2 may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by mediating glycosylation of KCNH2 (PubMed:9722534, PubMed:14525949). Has a role in maintenance of cochlear outer hair cell function (By similarity).By similarity2 Publications
Catalytic activityi
- α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl β-D-glucosyl phosphate = α-D-Glc-(1→2)-α-D-Glc-(1→3)-α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H+By similarityEC:2.4.1.256By similarity
GO - Molecular functioni
GO - Biological processi
- auditory receptor cell development Source: RGD
- dolichol-linked oligosaccharide biosynthetic process Source: InterPro
- potassium ion transmembrane transport Source: RGD
- protein N-linked glycosylation Source: GO_Central
- sensory perception of sound Source: RGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Protein family/group databases
CAZyi | GT59, Glycosyltransferase Family 59 |
Names & Taxonomyi
Protein namesi | Recommended name: Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseBy similarity (EC:2.4.1.256By similarity)Alternative name(s): Alpha-1,2-glucosyltransferase ALG10-A Alpha-2-glucosyltransferase ALG10-B Asparagine-linked glycosylation protein 10 homolog B Potassium channel regulator 1 |
Gene namesi | Name:Alg10b Synonyms:Kcr1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708500, Alg10b |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 27 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 28 – 64 | ExtracellularSequence analysisAdd BLAST | 37 | |
Transmembranei | 65 – 85 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 86 – 97 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 98 – 118 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 119 – 126 | ExtracellularSequence analysis | 8 | |
Transmembranei | 127 – 147 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 148 – 150 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 151 – 171 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 172 – 175 | ExtracellularSequence analysis | 4 | |
Transmembranei | 176 – 196 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 197 – 256 | CytoplasmicSequence analysisAdd BLAST | 60 | |
Transmembranei | 257 – 277 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 278 – 283 | ExtracellularSequence analysis | 6 | |
Transmembranei | 284 – 304 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 305 – 317 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 318 – 338 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 339 – 365 | ExtracellularSequence analysisAdd BLAST | 27 | |
Transmembranei | 366 – 386 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 387 – 392 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 393 – 413 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 414 – 436 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 437 – 457 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 458 – 473 | CytoplasmicSequence analysisAdd BLAST | 16 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215449 | 1 – 474 | Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseAdd BLAST | 474 |
Proteomic databases
PaxDbi | O88788 |
PRIDEi | O88788 |
PTM databases
PhosphoSitePlusi | O88788 |
Expressioni
Tissue specificityi
Highly expressed in brain, skeletal muscle, uterus, small intestine and liver. Moderately expressed in lung and kidney. Weakly expressed in heart and stomach.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000014511, Expressed in duodenum and 20 other tissues |
Interactioni
Subunit structurei
Interacts with KCNH1 and KCNH2.
2 PublicationsProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000019672 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ALG10 glucosyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2642, Eukaryota |
GeneTreei | ENSGT00390000012906 |
HOGENOMi | CLU_017053_1_0_1 |
InParanoidi | O88788 |
OMAi | CSTAMLR |
OrthoDBi | 476469at2759 |
PhylomeDBi | O88788 |
TreeFami | TF300150 |
Family and domain databases
InterProi | View protein in InterPro IPR016900, Alg10 |
PANTHERi | PTHR12989, PTHR12989, 1 hit |
Pfami | View protein in Pfam PF04922, DIE2_ALG10, 1 hit |
PIRSFi | PIRSF028810, Alpha1_2_glucosyltferase_Alg10, 1 hit |
i Sequence
Sequence statusi: Complete.
O88788-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE
60 70 80 90 100
GRFSLSQWDP MITTLPGLYL VSVGVVKPAS WILGWSEHVV CSIGMLRFVN
110 120 130 140 150
LLFSVGNFYL LYLLFRKIQP RNKASSSIQR ILSTLTLAVF PTLYFFNFLY
160 170 180 190 200
YTEAGSVFFT LFAYLMCLYG NHRTSALLGF CGFMFRQTNI IWAAFCAGHI
210 220 230 240 250
IAQKCSEAWK TELQKKKEER LPPAKGPLSE LRRVLQFLLM YSMSLKNLSM
260 270 280 290 300
LFLLTWPYML LLLAFFVFVV VNGGIVVGDR SSHEACLHFP QLFYFFSFTA
310 320 330 340 350
FFSFPHLLSP TKVKTFLSLV WKRRVQFSVI TLVSVFLVWK FTYVHKYLLA
360 370 380 390 400
DNRHYTFYVW KRVFQRHEIV KYLLVPAYMF AGWAVADSLK SKSIFWNLMF
410 420 430 440 450
FVCLVASTVP QKLLEFRYFI LPYIIYRLNM PLPPISRLVC ELGCYAVVNF
460 470
LTFYIFLNKT FQWSDSHDIQ RFMW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78090 mRNA Translation: AAC34249.1 |
RefSeqi | NP_620801.1, NM_139101.2 |
Genome annotation databases
Ensembli | ENSRNOT00000019672; ENSRNOP00000019672; ENSRNOG00000014511 |
GeneIDi | 245960 |
KEGGi | rno:245960 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78090 mRNA Translation: AAC34249.1 |
RefSeqi | NP_620801.1, NM_139101.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000019672 |
Protein family/group databases
CAZyi | GT59, Glycosyltransferase Family 59 |
PTM databases
PhosphoSitePlusi | O88788 |
Proteomic databases
PaxDbi | O88788 |
PRIDEi | O88788 |
Genome annotation databases
Ensembli | ENSRNOT00000019672; ENSRNOP00000019672; ENSRNOG00000014511 |
GeneIDi | 245960 |
KEGGi | rno:245960 |
Organism-specific databases
CTDi | 84920 |
RGDi | 708500, Alg10b |
Phylogenomic databases
eggNOGi | KOG2642, Eukaryota |
GeneTreei | ENSGT00390000012906 |
HOGENOMi | CLU_017053_1_0_1 |
InParanoidi | O88788 |
OMAi | CSTAMLR |
OrthoDBi | 476469at2759 |
PhylomeDBi | O88788 |
TreeFami | TF300150 |
Miscellaneous databases
PROi | PR:O88788 |
Gene expression databases
Bgeei | ENSRNOG00000014511, Expressed in duodenum and 20 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR016900, Alg10 |
PANTHERi | PTHR12989, PTHR12989, 1 hit |
Pfami | View protein in Pfam PF04922, DIE2_ALG10, 1 hit |
PIRSFi | PIRSF028810, Alpha1_2_glucosyltferase_Alg10, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AG10B_RAT | |
Accessioni | O88788Primary (citable) accession number: O88788 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families